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Citations for Mirus Bio :
451 - 500 of 784 citations for RPA kit reagent since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2021Quote: ... vCas9-4T1 cells were transfected with crRNA and tracrRNA according the manufactures protocol using TransIT-X2 transfecting reagent (Mirus) in 12 well plates ...
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bioRxiv - Microbiology 2021Quote: A549 ZAP−/−/− and DF-1 cells were cultured in 10 cm dish and transfected using LT1 reagent (Mirus lab) with 5 μg of huZAP-GFP DNA for 24 h ...
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bioRxiv - Microbiology 2021Quote: ... The pTM plasmids were transfected into H7-T7-IZ cells using TransIT®-LT1 Transfection Reagent (Mirus Bio LLC) following the manufacturer’s recommendations with a ratio ADN to transfection reagent of 1 to 3.
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bioRxiv - Microbiology 2021Quote: ... Wild-type/mutant MeV-M bearing a FLAG-tag and NiV-M bearing an HA-tag were cloned into pCMV and pcDNA3.1 respectively and transfected into cells using TrasnIT-LT1 transfection reagent (Mirus). VLPs were harvested 24 hours post-transfection ...
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bioRxiv - Microbiology 2021Quote: ... monolayers of BHK-T7 cells (4 × 105) cultured in 12-well plates were co-transfected using 2.5 μL of TransIT-LT1 transfection reagent (Mirus) per microgram of DNA plasmid ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... 50 ng of pcDNA3-PLPro-flipGFP-T2A-mCherry plasmid and 50 ng of protease expression plasmid pcDNA3.1-PLpro were added to each well in the presence of transfection reagent TransIT-293 (Mirus) according to manufacturer’s protocol ...
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bioRxiv - Biophysics 2021Quote: ... monolayers of BHK-T7 cells (4 × 105) cultured in 12-well plates were co-transfected using 2.5 μL of TransIT-LT1 transfection reagent (Mirus) per microgram of DNA plasmid ...
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bioRxiv - Cell Biology 2020Quote: ... 150 μL pre-warmed OPTI-MEM was placed in 1.5 mL tubes with 6 μL TransIT-LT1 transfection reagent (MIR 2300, Mirus), vortexed briefly ...
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bioRxiv - Molecular Biology 2022Quote: ... samples were transfected with 5 μg of the indicated FLAG-tagged TTP wild-type or mutant expression plasmids using TransIT 293 reagent according to the manufacturer’s protocol (Mirus). 48 hours after transfection ...
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bioRxiv - Microbiology 2022Quote: ... monolayers of BHK-T7 cells (4×105) cultured in 12-well plates were co-transfected using 2.5 μl of TransIT-LT1 transfection reagent (Mirus) per microgram of DNA plasmid ...
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bioRxiv - Cancer Biology 2020Quote: Lentivirus was generated by transfecting HEK39T cells with standard packaging vectors using TransIT®-LT1 Transfection Reagent (Mirus Bio). Viral supernatant was harvested 2-3 days after transfection and filtered through 0.44 µm PVDF filters and/or frozen prior to transduction.
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bioRxiv - Microbiology 2022Quote: ... purified replicon RNA was transfected into HeLa cells grown on 96 well plates using TransIT mRNA transfection reagent (Mirus), and the cells were incubated in the growth medium supplemented with 5µM of cell-permeable Renilla luciferase substrate EnduRen (Promega ...
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bioRxiv - Cell Biology 2022Quote: ... Ten 10cm plates were seeded with COS-7 cells and each plate was co-transfected 24 hours later with 4.08 μg MKLP1(1-711)-3xFLAG-Avi and 4.08 μg HA-BirA plasmids using TransIT-LT1 transfection reagent (Mirus), and Opti-MEM Reduced Serum Medium (Gibco) ...
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bioRxiv - Microbiology 2019Quote: ... monolayers of BHK-T7 cells (4 × 105) cultured in 12-well plates were co-transfected using 2.5 μL of TransIT-LT1 transfection reagent (Mirus) per microgram of DNA plasmid ...
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bioRxiv - Molecular Biology 2019Quote: ... cultured in Schneider’s Drosophila Medium containing 10% heat-inactivated FBS and 1X penicillin-streptomycin with the TransIT-LT 1 reagent (Mirus). Flag-CG30403 only (no bait ...
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bioRxiv - Microbiology 2019Quote: ... HEK 293 cells were transfected with YF-NS1-GFP using TransIT®-LT1 transfection reagent (Mirus Bio LLC, Belgium), according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2020Quote: ... or with 1μg of plasmid expressing TTFI-GFP under the full CMV promoter using Transit-X2 transfection reagent (Mirus) in Opti-MEM GlutaMAX reduced-serum medium (Life Technologies) ...
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bioRxiv - Cell Biology 2020Quote: ... adherent Sf21 cells were transfected with plasmids expressing ARIF-1 using TransIT-Insect transfection reagent (Mirus Bio, Madison, WI). At 3 d post-transfection ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... 50 ng pcDNA3-flipGFP-T2A-mCherry plasmid and 50 ng protease expression plasmid pcDNA3.1 were used each well in the presence of transfection reagent TransIT-293 (Mirus). 3 hrs after transfection ...
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bioRxiv - Microbiology 2019Quote: ... and 310 ng of pGL4.23-CMV/GP-A82V [15] using 6.25 µL of TransIT-LT1 Transfection Reagent (Mirus Bio). We changed media the next morning ...
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bioRxiv - Microbiology 2020Quote: ... monolayers of BHK-T7 cells (4 × 105) cultured in 12-well plates were co-transfected using 2.5 μL of TransIT-LT1 transfection reagent (Mirus) per microgram of DNA plasmid ...
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bioRxiv - Biochemistry 2021Quote: ... monolayers of BHK-T7 cells (4 × 105) cultured in 12-well plates were co-transfected using 2.5 μL of TransIT-LT1 transfection reagent (Mirus) per microgram of DNA plasmid ...
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bioRxiv - Cancer Biology 2019Quote: ... Lentivirus expressing RIPK3 shRNA was generated by transfecting HEK-293T cells in 6 well plate with a 1: 0.1: 1 ratio of pMDG2: pVSVG: pLKO.1 with TransIT-LT1 transfection reagent (Mirus). After filtering through 0.45 μm of cellulose acetate membrane (VWR ...
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bioRxiv - Molecular Biology 2019Quote: ... plus pMK1221-control or −GR shRNA (2.88 ug shCtrl or shGR, respectively) using TransIT-293 Transfection Reagent (Mirus Bio) as instructed by the manufacturer ...
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bioRxiv - Cancer Biology 2021Quote: ... pCMV-deltaR8.91 and pMD2.G-VSV-G into HEK293T cells using the TransIT-LT1 Transfection Reagent (Mirus Bio LLC). Virus-containing supernatants were collected 48 hr after transfection ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... 50 ng of pcDNA3-flipGFP-PLpro-T2A-mCherry plasmid and 50 ng of pcDNA3.1-SARS2-PLpro were used each well in the presence of transfection reagent TransIT-293 (Mirus) per manufacturer protocol ...
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bioRxiv - Immunology 2021Quote: ... Retroviral particles were produced by transfection of Platinum E (PLAT-E) cells with the TransIT-LT1 transfection reagent (Mirus) in Opti-MEM I reduced serum medium ...
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bioRxiv - Cell Biology 2022Quote: ... and 1.2 μg cytomegalovirus 8.91 vector were prepared for transfection using TransIT-293 Transfection Reagent (Mirus Bio; Madison, WI). Three days post transduction virus-containing supernatants were harvested and concentrated approximately 40-fold using Lenti-X Concentrator (Clontech ...
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bioRxiv - Microbiology 2022Quote: ... with pRL-EMCV-FL reporter plasmid and the indicated pCAGGS-based nsp1 expression plasmids using TransIT-LT1 reagent (Mirus). At 24 hours post-transfection ...
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bioRxiv - Zoology 2022Quote: ... Four µg of either plasmid were transfected into 6 × 105 C6/36 cells using TransIT-2020 transfection reagent (Mirus) for 4 h ...
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bioRxiv - Cell Biology 2023Quote: ... HeLa cells were arrested in S-phase with double-thymidine block and transfected in 24 well with 0,25 μg of vector 24 h with the TransIT-LT1 Transfection Reagent (Mirus), according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2023Quote: ... 0.5 μg of total plasmid DNA and 1.5 μL of TransIT ®-2020 Transfection Reagent (Mirus Bio, WI, USA) were mixed by gentle pipetting ...
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bioRxiv - Cell Biology 2023Quote: ... and 1.2 µg cytomegalovirus 8.91 vector were mixed and prepared for transfection using TransIT-293 transfection reagent (Mirus Bio) per the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... HEK cells were transfected with TCR expression vector and lentiviral transfer plasmids using TransIT-VirusGen Transfection Reagent (Mirus #MIR6700) following the manufacturer’s recommendation ...
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bioRxiv - Biophysics 2023Quote: ... STARD11-GFP WT or mutant plasmids were transfected using the TransIT-X2® Transfection Reagent (Mirus Bio, MIR 6000). Cells were fixed in 3% paraformaldehyde for 20 min before staining with antibodies diluted in a PBS solution of 1% BSA and 0.05% saponin ...
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bioRxiv - Biochemistry 2023Quote: ... plasmids were transfected in duplicate into HEK293T cells in a 48-well plate using LT-1 transfection reagent (Mirus) with the indicated plasmids ...
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bioRxiv - Microbiology 2023Quote: ... followed by transfection 6 hours post seeding using different EBOV plasmids and TransIT-LT1 transfection reagent (Mirus Bio LLC) according to manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2023Quote: ... were done by mixing 2.5 µg of plasmid DNA with 7.5 µl of TransIT-X2 reagent (Mirus, Madison, WI). The mixture was added to 105 CAF or PDAC cells cultured in serum-free and antibiotic-free media overnight.
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bioRxiv - Cell Biology 2024Quote: ... the packaging plasmids psPAX2 (UK1701) and pMD2.G (UK1700) at 10:7.5:5 ratio using TransIT-293 Transfection Reagent (MIR2704, Mirus) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2024Quote: Transfection of Capsaspora cells and stable cell line generation was done using the TransIT-X2 transfection reagent (Mirus Bio) as previously described (Phillips et al. ...
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bioRxiv - Genetics 2021Quote: ... Transfection of 6xMyc-tagged RBPJ constructs (pCS2-RBPJ-6xMyc, WT and AOS variants) was carried out with TransIT-293 transfection reagent (Mirus) following the manufacture’s instruction ...
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bioRxiv - Neuroscience 2021Quote: ... Cells were transfected at ∼60% confluency with 2.5 µg of the appropriate DNA construct using the TransIT-LT1 Transfection Reagent (Mirus; MIR2300) and cultured for an additional 2 days ...
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bioRxiv - Molecular Biology 2020Quote: BHK-21 cells were transfected in 25 cm2 flasks with 8 µg per flask of infectious clone-derived RNA using TransIT transfection reagent (Mirus) as described previously (32) ...
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bioRxiv - Microbiology 2019Quote: ... in 24-well plates were transfected with 0.5 µg of plasmid DNA per well using TransIT-LT1 transfection reagent (Mirus Bio) in OptiMEM (Gibco) ...
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bioRxiv - Genomics 2021Quote: ... and pMD2.G at a ratio of 9:8:1 by mass using TransIT-LT1 transfection reagent (Mirus MIR 2306) at a ratio of 3 μL transfection reagent per 1 μg plasmid ...
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bioRxiv - Microbiology 2021Quote: ... a 10x mix was prepared in OptiMEM containing the appropriate siRNA(s) and TransIT-X2 transfection reagent (MIR 600x, Mirus) in a 1:2 stoichiometry ...
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bioRxiv - Biophysics 2021Quote: ... δ and ε subunits in the ratio 2:1:1:1 (TransIT® 293 transfection reagent; Mirus Bio, Madison, WI). Electrophysiological experiments started ~48 hours post-transfection ...
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Potassium channel-driven bioelectric signaling regulates metastasis in triple-negative breast cancerbioRxiv - Cancer Biology 2021Quote: ... Constructs were mixed with 0.5μg helper (encoding gag/pol) and 0.1μg encoding coat protein (pMD2) along with 6μL TransIT-Lenti transfection reagent (MIR6603; Mirus Bio; Madison, WI) in 200uL Opti-MEM (31985062 ...
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bioRxiv - Cell Biology 2021Quote: ... Blasticidin (catalog no. 203350) was bought from EMD Chemicals/Millipore. TransIT-LT1 transfect reagent (catalog no. MIR2300) was obtained from Mirus Bio LLC ...
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bioRxiv - Cell Biology 2021Quote: Lentivirus were generated by transfecting HEK293T cells with standard packaging vectors using TransIT-LT1 Transfection Reagent (Mirus Bio, Madison, WI) or Lipofectamine 2000 (Invitrogen ...