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Citations for Mirus Bio :
551 - 600 of 784 citations for RPA kit reagent since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... cells were plated in 6-well dishes and transfected with mEGFP-tagged DNAJC17 or deletion mutants using TransIt-LT1 reagent at a 3:1 ratio (Mirus). Cells were then trypsinized and replated on 4-well chambered coverglass (LabTek ...
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bioRxiv - Cell Biology 2024Quote: ... 1mM NEAA and penicillin–streptomycin) were transfected with 500μl Opti-MEM containing 30 μl TransITβ-LT1 Transfection Reagent (Mirus, MIR2305), 10μg pLV-tetO-Tfap2c ...
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bioRxiv - Molecular Biology 2024Quote: ... constructs with inserted genes were co-transfected with vectors expressing lentiviral gag/pol (psPAX2) and vsvg (pMD2.G) genes in HEK293T cells to produce lentiviruses by TransIT-LT1 transfection reagent (Mirus). Supernatants containing virus particles were collected at 48 hours and 72 hours following transfection ...
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bioRxiv - Cell Biology 2024Quote: ... Generation of lentivirus was performed by transfecting HEK293T cells with standard delta VPR and VSVG packaging vectors paired with TransIT-LTI Transfection Reagent (MIR2305, Mirus). Lentivirus was harvested 72 hrs following transfection and frozen at -80°C.
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bioRxiv - Molecular Biology 2024Quote: BHK-21 cells were transfected in 25 cm2 flasks with 8 µg per flask of infectious clone-derived RNA using TransIT transfection reagent (Mirus) as described previously (35) ...
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bioRxiv - Microbiology 2021Quote: ... the pCMV(CAT)T7-SB100 plasmid (encoding transposase required to generate stable transgene-expressing cell lines) was co-transfected with pSBbi-BH hACE2 using TransIT-LT1 Transfection Reagent (Mirus Bio). pCMV(CAT)T7-SB100 was a kind gift from Zsuzsanna Izsvak (Addgene #34879) ...
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bioRxiv - Developmental Biology 2020Quote: ... The HEK 293 cells were cultured at 37°C in DMEM media until they 80% confluency and transfected using TransIT®-LT1 Transfection Reagent (Mirus) at 37°C for 24h ...
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bioRxiv - Cell Biology 2022Quote: Viral particles were produced by transfection of 293FT cells in 6-well plates with 3 μg of total DNA and 8 mL TransIT-293 Transfection Reagent (Mirus Bio) per well ...
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Prom1 and Notch regulate ciliary length and dynamics in multiciliated cells of the airway epitheliumbioRxiv - Cell Biology 2022Quote: ... The VSV-G pseudotyped lentivirus was packaged by 5 plasmid co-transfection of HEK293T cells using TansIT Transfection Reagent (Mirus Bio) in 15 cm culture dishes (The protocol describing production of lentiviral particles can be found at http://www.www.bu.edu/dbin/stemcells/protocols) ...
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bioRxiv - Molecular Biology 2021Quote: ... HEK293T-GFP cells were transiently transfected with wild-type and mutant spike plasmids using TransIT-X2 transfection reagent (Mirus #MIR 6000) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2019Quote: Lentivirus was produced by transfecting HEK293T with standard packaging vectors using the TransIT-LT1 Transfection Reagent (MIR 2306; Mirus Bio LLC). Viral supernatant was collected at 48h and 72 h after transfection ...
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bioRxiv - Microbiology 2021Quote: ... px459 CRISPR/Cas9 plasmids with or without gRNA sequence were transfected into HEK293T using TransIT-LT1 transfection reagent (Mirus, MIR 2306). Puromycin (1 μg/mL ...
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bioRxiv - Bioengineering 2021Quote: ... 1×105 MC38 cells were transfected with gRNA-ligated eSpCas9 plasmids for 48 hours using TransIT-LT1 transfection reagent (Mirus Bio) in Opti-MEM (Thermo Fisher ...
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bioRxiv - Microbiology 2021Quote: ... TK-Renilla luciferase reporter plasmid (as an internal control) and the desired expression vectors or empty vector (EV) using TransIT-LT1 transfection reagent (Mirus Bio), according to the manufacturer’s instruction ...
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bioRxiv - Cancer Biology 2020Quote: ... were transfected at a 4:3:1 ratio using TransIT293 reagent into T225 flasks as indicated by the manufacturer (Mirus Bio). For individual sgRNA preparations ...
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bioRxiv - Biochemistry 2021Quote: ... and 7 μl of VSV-G pseudovirus in 500 μl of 1X opti-MEM reduced serum free media using TransIT-Pro® as a transfection reagent (Mirus). After 4 hr of transfection ...
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bioRxiv - Molecular Biology 2020Quote: ... to be integrated into the genomic FRT site was cloned into the pcDNA5/FRT/TO vector backbone and transfected together with pOG44 Flp-In recombinase in a 9:1 ratio using either Trans-IT 293 transfection reagent (Mirus, USA) or Lipofectamine 3000 (Thermo Fisher Scientific ...
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bioRxiv - Biophysics 2020Quote: 1μg of recombinant Plexin-B1 FL WT pCDNA 3.1 plasmid or Plexin-B1 FL mutants pCDNA 3.1 (as described above) was transfected to 80% confluent COS 7 cells on coverslips using 3 μL Trans IT 2020 transfection reagent (Mirus Bio) for 48 hours at 37 °C ...
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bioRxiv - Biophysics 2022Quote: CHO-GFP-CCR5 cell lines were generated by transfecting CHO-K1 with a pCDNA3.1 Hygro-GFP-CCR5 construct using the TransIT-X2 transfection reagent from Mirus (MIR 6003). CCR5 expression was maintained by culturing cells in the presence of 400 µg/ml hygromycin and GFP-CCR5 expressing cells were isolated for single-cell cloning through the process of serial dilution cloning ...
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bioRxiv - Cancer Biology 2022Quote: ... Iso-2 and Cdk1 and its mutant plasmids were transfected into the cells using TransIT-X2 transfection reagent (Mirus, cat# MIR6000). siRNA oligonucleotides specific for Rad 51 were purchased from Sigma (ESIRNA HUMAN RAD51 EHU045521) ...
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bioRxiv - Microbiology 2020Quote: ... cells were transfected overnight with either a control plasmid (PLX) or TMPRSS2 (PLX-TMPRSS2) via TransIT LT-1 transfection reagent (Mirus Bio) in 100 µL of OptiMEM per well ...
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bioRxiv - Immunology 2020Quote: ... The cells were transfected with the sgRNA transfer plasmids (MSCV-U6-sgRNA-IRES-Thy1.1) using TransIT-293 transfection reagent per the manufacturer’s protocol (Mirus, Cat# MIR 2700). The following morning ...
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bioRxiv - Microbiology 2021Quote: ... was diluted to 10 μg/ml and incubated for 60 minutes with a MIRUS Label-IT rhodamine TM reagent (Mirus Bio) according to the manufacturer’s recommendations ...
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bioRxiv - Immunology 2021Quote: Mt1-/-Mt2-/- BMDMϕ were transfected with pCMV6-Ac-GFP control vector or pCMV6-Ac-MT3-GFP vector using the LT1 transfection reagent (Mirus Bio) in RPMI media containing 10% serum without antibiotics as per the manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... Mϕ were transfected with the transfection complex (50 μl) of siRNA and TransIT-TKO® (0.5 %) transfection reagent (Mirus Bio™) in 500 μl of complete RPMI 1640 medium without antibiotics as per the manufacturer’s instructions ...
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bioRxiv - Genomics 2022Quote: ... in HK-2 human proximal tubule cells at approximately 70% confluency in 96-well plates by using TransIT-2020 Reagent (Mirus, #5404), following the manufacturer’s protocol ...
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bioRxiv - Immunology 2022Quote: ... and NP proteins (23) were transfected into 50% confluent HEK293T cells in 6-well plates with TransIT-LT1 transfection reagent (Mirus Bio) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2019Quote: ... ZIKV was rescued by transfection of the infectious clone in semi-confluent HEK-293T cells using TransIT-LT1 transfection reagent (Mirus Bio). For both viruses ...
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bioRxiv - Microbiology 2019Quote: ... and varying input (50 ng for 5-site screen and 15 ng for 4-site screen) of MxA plasmids were co-transfected into HEK 293T cells using the reagent TransIT-LT1 (Mirus Bio) in a 96-well plate format ...
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bioRxiv - Molecular Biology 2019Quote: ... a 10 cm dish of cells was transfected with 9 µg of Flp-Recombinase (plasmid pOG44) and 1 µg of a specific pcDNA5/FRT plasmid using TransIT-293 transfection reagent (Mirus, MIR2705). 48 hours after transfection ...
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bioRxiv - Biochemistry 2019Quote: ... The plasmid was co-transfected into S2 insect cells with 10:1 molar excess of pCoBlast using TransIT-Insect transfection reagent (Mirus Bio). Stable cell lines were selected with 100 µg/mL blasticidin (Cambridge Bioscience) ...
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bioRxiv - Molecular Biology 2021Quote: psiCHECK2-Eef2TOP-RLCP-HCVIRES-FL and psiCHECK2-Eef2TOPm-RLCP-HCVIRES-FL plasmids were transfected into cells with TransIT-293 Reagent (Mirus, MIR2704). After 24 h of incubation ...
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bioRxiv - Cell Biology 2020Quote: ... a plasmid containing a cAMP response element binding site driving firefly luciferase was transfected with a control plasmid containing a CMV promotor driving renilla luciferase expression using the TransiT-X2 transfection reagent (Mirus Bio) 48 hours after the siRNA transfection ...
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bioRxiv - Cell Biology 2021Quote: ... and 1.2 μg cytomegalovirus 8.91 vector were mixed and prepared for transfection using TransIT-293 Transfection Reagent (Mirus Bio, MIR 2705). Following transfection ...
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bioRxiv - Cancer Biology 2021Quote: ... we co-transfected the cells with 50 ng of pMIR-REPORT constructs containing the 3’UTR sequences together with 50 ng of a Renilla housekeeping control plasmid and 100 nM of miR-424 or miR-503 mimics or a miRNA negative control (Dharmacon #C-300717-05, #C-300841-05, or #CN-001000-01-05, respectively) using the TransIT-X2 reagent (Mirus 6003). 24 hours after transfection ...
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bioRxiv - Cell Biology 2020Quote: ... GFP-tagged E-cadherin or with constructs encoding E-cadherin and TNFα RUSH system using TransIT-LT1 Reagent (Mirus, MIR 2305) according to the manufacturer’s instructions.
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bioRxiv - Microbiology 2021Quote: ... Maxiprep purified plasmids were transfected into HEK293FT cells using PEI or into ACH2 / U1 leukocytes via TransIT-Jurkat reagent (Mirus #2120). Supernatants were collected 72 hours after media replacement (PEI ...
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bioRxiv - Microbiology 2020Quote: Viral particles were produced by transfection of 293FT cells in 6-well plates with 3 μg DNA and 8 μl TransIT-293 Transfection Reagent (Mirus Bio) per well ...
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bioRxiv - Immunology 2021Quote: ... a gag-pol packaging construct (0.65 μg/well) and the relevant vector construct (1 μg/well) were transfected using the TransIT®-LT1 Transfection Reagent (MIR 2306, Mirus). Two days after transfection the soups containing the viruses were collected and filtered.
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bioRxiv - Cell Biology 2021Quote: ... C-terminal GFP-2x Strep-tagged full length and truncation versions of CEP90 were cloned into pLVX-EF1α-IRES-Puro backbone and transfected into 293T cells using TransIT-293 transfection reagent (Mirus Bio) according to manufacturer guidelines.
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bioRxiv - Genomics 2021Quote: ... and packaging plasmids for expression of HIV-1 gag/pol and rev (+/-tat) and VSV-G envelope protein using either TransIT®-LT1 Transfection Reagent (Mirus) or Polyethylenimine (Polysciences ...
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bioRxiv - Microbiology 2021Quote: ... Cells were transfected with 250 ng of a pCDNA3.1(+) vector encoding for the SARS-CoV-2 spike protein using the TransIT®-LT1 Transfection Reagent (Mirus Bio). Six hours post transfection (p.t.) ...
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bioRxiv - Microbiology 2021Quote: ... with Benin 97/1 (Figure 1A) and then transfection with pΔEP402R-VP72GUS using the TransIT-LT1 transfection reagent (Mirus Bio, USA). In the presence of X-Gluc ...
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bioRxiv - Microbiology 2020Quote: ... Confluent monolayers of BHK-T7 cells in 12-well plates were co-transfected using TransIT-LT1 transfection reagent (Mirus Bio LLC) with 0.27 μg of each of eleven plasmid constructs representing the SA11 rotavirus genome and of each of two plasmids encoding vaccinia virus mRNA capping enzymes ...
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bioRxiv - Microbiology 2022Quote: ... (1.2 μg of each) and C3P3-G1 (0.8 μg) were mixed with Trans-IT LT1 transfection Reagent (12.8 μl) (Mirus Bio, cat. #MIR2305) in OPTI-MEM I Reduced Serum Medium (150 μl ...
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bioRxiv - Molecular Biology 2022Quote: ... 0.2 μg pVSVG per 1 reaction in a 6-well plate well) into HEK 293T cells with TransIT-LT1 transfection reagent (Mirus #2304) following manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2022Quote: ... and 1.5 μg of lentiviral plasmid were added into a microcentrifuge tube with 7.5 μL of Mirus TransIT-Lenti transfection reagent (Mirus Bio, 6604). Once all plasmids were pooled together with the transfection reagent ...
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bioRxiv - Developmental Biology 2022Quote: ... Lentiviral vector was produced by transforming the produced LARRY plasmid into HEK293T cells using the Trans-IT 293 transfection reagent (Mirus #MIR2700). For each of eighteen 10 cm dishes of HEK293T cells ...
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bioRxiv - Genomics 2022Quote: ... Donor plasmids containing genes of interests were co-transfected with φC31-bacteriophage derived integrase in H11 V6.5 mESCs using TransIT-X2 Transfection Reagent (Mirus Bio, MIR6003) according to the provider’s instructions ...
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bioRxiv - Synthetic Biology 2022Quote: ... and transfected with a total of 2.5 μg of SSG-encoding DNA/well using TransIT-2020 Transfection Reagent (Mirus Bio, #MIR5400) following the manufacturer’s recommendations ...