Labshake search
Citations for Mirus Bio :
151 - 200 of 242 citations for 7 Chloro 1 3 dihydro 5 phenyl 2H 1 4 benzodiazepin 2 thione since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Molecular Biology 2020Quote: ... to be integrated into the genomic FRT site was cloned into the pcDNA5/FRT/TO vector backbone and transfected together with pOG44 Flp-In recombinase in a 9:1 ratio using either Trans-IT 293 transfection reagent (Mirus, USA) or Lipofectamine 3000 (Thermo Fisher Scientific ...
-
bioRxiv - Microbiology 2020Quote: ... cells were transfected overnight with either a control plasmid (PLX) or TMPRSS2 (PLX-TMPRSS2) via TransIT LT-1 transfection reagent (Mirus Bio) in 100 µL of OptiMEM per well ...
-
bioRxiv - Immunology 2022Quote: Monolayers of BSC-40 cells were infected with parental IHD-J at an MOI of 0.01 PFU/cell and transfected 1 hour later with 10 μg of DNA of plasmid pCyA-ISG15GG (pCyA was kindly provided by Mariano Esteban) using LT-1 (Mirus Bio) according to manufacturer’s recommendations ...
-
bioRxiv - Molecular Biology 2019Quote: ... a 10 cm dish of cells was transfected with 9 µg of Flp-Recombinase (plasmid pOG44) and 1 µg of a specific pcDNA5/FRT plasmid using TransIT-293 transfection reagent (Mirus, MIR2705). 48 hours after transfection ...
-
bioRxiv - Biochemistry 2019Quote: ... The plasmid was co-transfected into S2 insect cells with 10:1 molar excess of pCoBlast using TransIT-Insect transfection reagent (Mirus Bio). Stable cell lines were selected with 100 µg/mL blasticidin (Cambridge Bioscience) ...
-
bioRxiv - Immunology 2021Quote: ... a gag-pol packaging construct (0.65 μg/well) and the relevant vector construct (1 μg/well) were transfected using the TransIT®-LT1 Transfection Reagent (MIR 2306, Mirus). Two days after transfection the soups containing the viruses were collected and filtered.
-
bioRxiv - Genomics 2021Quote: ... and packaging plasmids for expression of HIV-1 gag/pol and rev (+/-tat) and VSV-G envelope protein using either TransIT®-LT1 Transfection Reagent (Mirus) or Polyethylenimine (Polysciences ...
-
bioRxiv - Microbiology 2021Quote: ... with Benin 97/1 (Figure 1A) and then transfection with pΔEP402R-VP72GUS using the TransIT-LT1 transfection reagent (Mirus Bio, USA). In the presence of X-Gluc ...
-
bioRxiv - Molecular Biology 2022Quote: ... 0.2 μg pVSVG per 1 reaction in a 6-well plate well) into HEK 293T cells with TransIT-LT1 transfection reagent (Mirus #2304) following manufacturer’s protocol ...
-
bioRxiv - Microbiology 2023Quote: ... MRC-5 cell monolayers were mock-transfected or transfected with sHCoV genomic RNA (1 μg/ml) using TransIT-mRNA Transfection Kit (Mirus Bio) at 33°C ...
-
bioRxiv - Microbiology 2023Quote: RBP-overexpressing Huh7 cells were generated by transfection of RBP expression plasmids using TransIT LT-1 transfection reagent (Mirus, WI, USA). Briefly ...
-
bioRxiv - Microbiology 2019Quote: ... 100ng of RIG-I agonist derived from the 3’-UTR of hepatitis C virus (55) was transfected per 1e6 cells using TransIT-mRNA transfection kit (Mirus). For stimulation of MDA5 signaling ...
-
bioRxiv - Biochemistry 2020Quote: ... The following day they were transfected with 6 μg Gag plasmid + 6 μg vector plasmid + 3 μg pCMV-Rev (to support nuclear export of vector RNA) using Transit-293 (Mirus) following the manufacturer’s protocol ...
-
bioRxiv - Microbiology 2019Quote: ... 100ng of RIG-I agonist derived from the 3’-UTR of hepatitis C virus (19) was transfected per 1e6 cells using TransIT-mRNA transfection kit (Mirus). For stimulation of MDA5 signaling ...
-
bioRxiv - Cell Biology 2020Quote: ... cells were transiently transfected with 3 µg pE-hABCA3-eGFP plasmid (hABCA3-eGFP expression controlled by CMV promoter) in Trans-IT LT1 transfection reagent (Mirus). 48 h post-transfection the medium was removed ...
-
bioRxiv - Molecular Biology 2023Quote: ... reporter plasmid delivery (3 µg/plate for cFSHr and 0.3 µg/plate for cLHr) was carried out with TransIT-X2® System (Mirus). The cells were serum-starved for 16 h ...
-
bioRxiv - Immunology 2021Quote: ... and replication-competent HIV-1 were produced by transfection into 293T cells with the TransIT-Lenti transfection reagent (Mirus Bio, Madison, WI) at a ratio of 1:3 (DNA ...
-
bioRxiv - Microbiology 2021Quote: ... and a full-length plasmid containing the chimeric LLOV genome (1 μg) using TransIT®-LT1 per manufacturer’s recommendations (Mirus Bio LLC). Media was changed approximately 18 hours post-transfection and cells were monitored for cytopathic effect (CPE ...
-
bioRxiv - Molecular Biology 2021Quote: ... or control siRNAs (siGENOME Non-Targeting siRNA Pool #1, D-001206-13-20) using the TransIT-X2 Dynamic Delivery System (Mirus Bio, MIR6003) for 72 h.
-
bioRxiv - Microbiology 2022Quote: ... cells were transfected with 1 µg of DDX60 wt or mutant constructs using TransIT®-LT1 Transfection Reagent (Mirus Bio MIR 2300) at a 1:3 (µg to µl ...
-
bioRxiv - Cell Biology 2019Quote: ... 250 ng lentiviral vector was co-transfected with a mix of third-generation lentivirus packaging vectors (250 ng total) using Mirus LT-1 transfection reagent (Mirus Bio LLC). Three days post-transfection ...
-
bioRxiv - Cancer Biology 2020Quote: ... Lentiviral vectors were incubated with 3rd generation viral packaging vectors (pCMV-VSV-G, pMDLg/pRRE, pRSV-Rev) and LT-1 (Mirus, MIR-2305) in OPTI-MEM media for 30 minutes as described by the provider’s instructions ...
-
bioRxiv - Microbiology 2022Quote: Recombinant HIV-1 variants were prepared by transfection of the pNL4.3 mutants into HEK293T cells using TransIT-293 (Mirus, Houston, TX, USA) and Opti-MEM I medium (Thermo Fisher ...
-
bioRxiv - Neuroscience 2022Quote: ... and eIF2αS/S or eIF2αA/A MEF cells were transiently transduced with TauRD(P301S)-YFP plasmid using TransIT LT-1 Transfection reagent (#MIR2020, Mirus Bio LLC) and virally transduced by lab-prepared lentiviral infection following manufacturer’s instruction and incubated for 2 days ...
-
bioRxiv - Cell Biology 2022Quote: Viral particles were produced by transfection of 293FT cells in 6-well plates with 3 μg of total DNA and 8 mL TransIT-293 Transfection Reagent (Mirus Bio) per well ...
-
bioRxiv - Systems Biology 2021Quote: ... torque teno virus hypothetical protein or human respirovirus 3 D protein were transfected into the cells using TransIT-LT1 (Mirus Bio) according to manufacturer’s protocol ...
-
bioRxiv - Microbiology 2020Quote: Viral particles were produced by transfection of 293FT cells in 6-well plates with 3 μg DNA and 8 μl TransIT-293 Transfection Reagent (Mirus Bio) per well ...
-
bioRxiv - Microbiology 2022Quote: ... were seeded into 6-well plates and transfected the next day with 200 ng pcDNA4/TO-RNR-3×FLAG using TransIT-LT1 (Mirus #2304). A3B-EGFP or A3A-EGFP expression was induced 6 hours after transfection with 50 ng/mL doxycycline ...
-
bioRxiv - Microbiology 2022Quote: Approximately 250,000 293T cells were seeded in 6-well plates and transfected the next day with 100 ng pcDNA5/TO-A3-EGFP and 200 ng pcDNA4/TO-RNR-3×FLAG using TransIT-LT1 (Mirus #2304). Cells were harvested ~24 hours after transfection and resuspended in 500 µl of lysis buffer (50 mM Tris-HCl pH 7.4 ...
-
bioRxiv - Biochemistry 2022Quote: ... in 12-well plates and were transfected with 3 μg capped in vitro transcribed DENV replicon RNA using the TransIT-mRNA Transfection Kit (Mirus Bio) according to the manufacturer’s instructions ...
-
bioRxiv - Biochemistry 2022Quote: ... BHK-21 cells cultured in 12-well plates were transfected with 3 μg capped in vitro transcribed DENV2 RNAs using the TransIT-mRNA transfection kit (Mirus Bio) according to the manufacturer’s instructions ...
-
bioRxiv - Microbiology 2023Quote: ... Huh7 cells in 6- well plates were transfected with 3 μg of each in vitro transcribed RNA using the TransIT mRNA transfection kit (Mirus Bio) as previously described (33) ...
-
bioRxiv - Microbiology 2021Quote: ... Virus inoculum was removed after 1 h and cells were transfected with a mixture of plasmids using TransIT-LT1 (Mirus Bio # MIR 2300), including pVSV-eGFP-deltaG_SARS-CoV-2 Spike (5 µg) ...
-
bioRxiv - Immunology 2019Quote: 100 ug of pCMV6 plasmid was labeled with 55 ul of Cy5 Label-IT kit for 1 hour at 37°C (Mirus Bio, Madison, WI). The labeled plasmid was purified by ethanol precipitation ...
-
bioRxiv - Systems Biology 2020Quote: ... followed by incubation with 10fold diluted Label IT Amine Modifying Reagent in 1× Labeling Buffer A from Label IT Nucleic Acid Modifying Reagent (Mirus Bio MIR 3900) at room temperature for 45 minutes ...
-
bioRxiv - Systems Biology 2021Quote: ... followed by incubation with tenfold diluted Label IT amine modifying reagent in 1× labelling buffer A from Label IT nucleic acid modifying reagent (Mirus Bio MIR 3900) at room temperature for 45 minutes ...
-
bioRxiv - Genomics 2021Quote: ... and the appropriate pLKO-derived vector (at ratios of 8 µg, 1 µg, and 8 µg, respectively, per 15 cm dish) with Trans-LT1 (Mirus Bio, Madison WI), according to the manufacturer’s instructions ...
-
bioRxiv - Immunology 2024Quote: ... 106 293T cells in a 2-mL culture volume were seeded overnight in a 35-mm dish before transfection using the TransIT LT-1 transfection reagent (Mirus Bio, Madison, WI). For each dish ...
-
bioRxiv - Microbiology 2024Quote: ... 106 293T cells in a 2-mL culture volume were seeded overnight in a 35-mm dish before transfection using the TransIT LT-1 transfection reagent (Mirus Bio, Madison, WI). For each dish ...
-
bioRxiv - Cell Biology 2020Quote: ... Cells were transfected with 5 μg of pACT-GFP-actin using TransIT-Insect transfection reagent (Mirus Bio) and incubated for 2 d at 28°C in Grace’s media with 10% FBS and antibiotics (100 μg/ml penicillin/streptomycin and 0.25 μg/ml Amphotericin B) ...
-
bioRxiv - Microbiology 2021Quote: ... monolayers of BHK-T7 cells (4 × 105) cultured in 12-well plates were co-transfected using 2.5 μL of TransIT-LT1 transfection reagent (Mirus) per microgram of DNA plasmid ...
-
bioRxiv - Biophysics 2021Quote: ... monolayers of BHK-T7 cells (4 × 105) cultured in 12-well plates were co-transfected using 2.5 μL of TransIT-LT1 transfection reagent (Mirus) per microgram of DNA plasmid ...
-
bioRxiv - Microbiology 2022Quote: ... monolayers of BHK-T7 cells (4×105) cultured in 12-well plates were co-transfected using 2.5 μl of TransIT-LT1 transfection reagent (Mirus) per microgram of DNA plasmid ...
-
bioRxiv - Microbiology 2019Quote: ... monolayers of BHK-T7 cells (4 × 105) cultured in 12-well plates were co-transfected using 2.5 μL of TransIT-LT1 transfection reagent (Mirus) per microgram of DNA plasmid ...
-
bioRxiv - Microbiology 2020Quote: ... monolayers of BHK-T7 cells (4 × 105) cultured in 12-well plates were co-transfected using 2.5 μL of TransIT-LT1 transfection reagent (Mirus) per microgram of DNA plasmid ...
-
bioRxiv - Biochemistry 2021Quote: ... monolayers of BHK-T7 cells (4 × 105) cultured in 12-well plates were co-transfected using 2.5 μL of TransIT-LT1 transfection reagent (Mirus) per microgram of DNA plasmid ...
-
bioRxiv - Biophysics 2022Quote: ... with 600 – 1000 ng DNA and 2 μL TransIT-LT1 transfection reagents (Mirus Bio Corporation) following the manufacturer’s protocol ...
-
bioRxiv - Microbiology 2022Quote: 3×105cells were seeded in 6-well plates and transfected with 3CLpro plasmids via TransIT-PRO (Mirus Bio LLC, Madison, WI, USA) and incubated overnight ...
-
bioRxiv - Cancer Biology 2019Quote: ... DNA from the Flag-tagged ATOX-1 plasmid (provided by Prof. T. Fukai) was reverse transfected with Opti-MEM and TransIT®-LT1 Transfection Reagent (Mirus Bio LLC, US) according to manufacturer’s instructions ...
-
bioRxiv - Cell Biology 2023Quote: ... was then transfected into SH-SY5Y cells (50-60% confluent) in a 6-well plate using TransIT LT-1 reagent (Mirus Bio, Cat# MIR-2300). After 48 hr ...