Labshake search
Citations for Mirus Bio :
151 - 200 of 206 citations for Sulfur Free Cobalt Metallo Organic Standard Co @ 5000 µg g in Hydrocarbon Oil since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cell Biology 2020Quote: ... cells were transiently transfected with 3 µg pE-hABCA3-eGFP plasmid (hABCA3-eGFP expression controlled by CMV promoter) in Trans-IT LT1 transfection reagent (Mirus). 48 h post-transfection the medium was removed ...
-
bioRxiv - Molecular Biology 2022Quote: ... and 1.5 µg of a pLKO.1-Hygro or lentiCas9-Blast plasmid using the TransIT-LT1 Transfection Reagent (Mirus Bio) as per the manufacturer’s protocol ...
-
bioRxiv - Molecular Biology 2023Quote: ... plus 10 µg of the vector of interest using Mirus TransIT-LT1 transfection reagent (Mirus Bio LLC, cat # MIR 2305). Medium was refreshed 16 h post-transfection ...
-
bioRxiv - Molecular Biology 2024Quote: BHK-21 cells were transfected in 25 cm2 flasks with 8 µg per flask of infectious clone-derived RNA using TransIT transfection reagent (Mirus) as described previously (35) ...
-
bioRxiv - Immunology 2024Quote: ... viral RNA isolated from infected BHK cells was transfected into cells at a concentration of 500 µg/ml using TransIT-mRNA transfection kit (Mirus). RABVLe RNA (2 pmol/ml ...
-
bioRxiv - Microbiology 2023Quote: ... Medium was changed 24 h later to 10 ml of 5 % FBS/DMEM and changed again 48 h later to 10 ml of 5 % FBS/DMEM containing 1 µg/ml of puromycin (Mirus). Cells were incubated in selection medium for 72 h ...
-
Nucleoprotein reassortment enhanced transmissibility of H3 1990.4.a clade influenza A virus in swinebioRxiv - Microbiology 2023Quote: ... 1 µg of each plasmid was mixed with 18 µl of TransIT-LT1 transfection reagent (Mirus Bio LLC, Madison, WI). The mixture was incubated for 45 min and then used to overlay the 293T/MDCK cells overnight ...
-
bioRxiv - Microbiology 2023Quote: ... in 24-well plates were transfected with 0.5 µg of plasmid DNA per well using TransIT-LT1 transfection reagent (Mirus Bio) in OptiMEM (Gibco) ...
-
bioRxiv - Molecular Biology 2024Quote: ... 70% confluent) were transfected with 10 µg pMT-FLAG-Ago1 plasmid using 20 µl TransIT-Insect Transfection Reagent (Mirus Bio) according to manufacturer’s instructions ...
-
bioRxiv - Genomics 2024Quote: ... mRNA transfections were performed using 0.5 µg of mRNA in 6-well plates using TransIT-mRNA Transfection Kit (Mirus Bio) according to the manufacturer’s instructions ...
-
bioRxiv - Cell Biology 2024Quote: ... HEK293T cells grown in four 10cm dishes were transfected with 6 µg CAT-tail mimetic DNA using TransIT293 (Mirus Bio). Cells were expanded for 72 h and then harvested in ice cold PBS ...
-
bioRxiv - Cell Biology 2024Quote: ... HEK293T cells in 6-well plates were transiently transfected with 1 µg of PSMB-3xFLAG constructs using TransIT-293 transfection reagent (Mirus). To analyze interactions between KCMF1-3xFLAG mutants and endogenous proteins ...
-
bioRxiv - Cell Biology 2020Quote: ... together with the packaging plasmids psPAX2 (UK1701) and pMD2.G (UK1700) at a 10:7.5:5 ratio using TransIT-293 reagent (Mirus). The supernatant containing the viral particles was collected 48 hrs after transfection ...
-
bioRxiv - Cell Biology 2022Quote: ... and 1 μg PMD2.G packaging plasmids using 25 μl TransIT®-LT1 Transfection Reagent (Cat# MIR 2306, Mirus). After 72 h of transfection ...
-
bioRxiv - Evolutionary Biology 2024Quote: ... and 333 ng of pMD2.G per well) using 3 μL of Trans-IT 293T transfection reagent (Mirus Bio) per μg of DNA ...
-
bioRxiv - Genomics 2024Quote: ... and after 24 hours transfected with 0.5 µ g variant-encoding plasmid using TransIT transfection reagent (Mirus Bio, #MIR6606). 24 hours after transfection ...
-
bioRxiv - Cell Biology 2024Quote: ... Lentiviral particles were generated by co-transfection of HEK 293T packaging cells with a lentiviral expression construct containing dCas9-KRAB and packaging constructs pMD VSV-G and pCMV Δ8.91 at a ratio of 1:0.7:0.3 using TransIT-293 transfection reagent (Mirus Bio, 2704). Media containing the lentivirus were collected 48 h post-transfection ...
-
bioRxiv - Biochemistry 2024Quote: ... Two samples each were treated with Opti-MEM with 2 µg of transfection DNA (either pJG01, or negative control without DNA) along with TransIT-X2 transfection reagent (Mirus Bio) premixed and allowed to incubate 5 minutes at room temperature ...
-
bioRxiv - Immunology 2023Quote: ... were seeded in 15-cm2 dishes at a density of 10 × 106 cells per dish and the following day were transfected with 25 µg of spike expression plasmid with TransIT-Lenti (Mirus, 6600) according to the manufacturer’s instructions ...
-
bioRxiv - Molecular Biology 2024Quote: ... S2 cells were transfected with 10 µg of plasmid DNA using TransIT-Insect Transfection Reagent (MIR 6100: Mirus Bio, WI, USA). Following 3.5 d of incubation at 25°C ...
-
bioRxiv - Microbiology 2024Quote: ... The transfection mix was prepared by mixing 2 ml Opti-MEM medium with 2.5 µg of each of the 8 RG plasmids and 60 µl TransIT® -LT1 transfection reagent (Mirus Bio) and incubation at room temperature (RT ...
-
bioRxiv - Biophysics 2024Quote: ... Cells were grown to 60-80% confluency in 10 cm dishes and transfected using 7 µg plasmid DNA and 7 µL TransIT-Pro reagent (Mirus Bio). The next day ...
-
bioRxiv - Genomics 2021Quote: ... and pMD2.G at a ratio of 9:8:1 by mass using TransIT-LT1 transfection reagent (Mirus MIR 2306) at a ratio of 3 μL transfection reagent per 1 μg plasmid ...
-
bioRxiv - Synthetic Biology 2021Quote: ... with a pHR’SIN:CSW transgene expression vector and the viral packaging plasmids pCMVdR8.91 and pMD2.G using Mirus Trans-IT Lenti (Mirus #MIR6606). Primary T cells were thawed the same day ...
-
Antigen-dependent inducible T cell reporter system for PET imaging of breast cancer and glioblastomabioRxiv - Synthetic Biology 2022Quote: ... with a pHR’SIN:CSW transgene expression vector and the viral packaging plasmids pCMVdR8.91 and pMD2.G using Mirus Trans-IT Lenti (Mirus #MIR6606). Primary T cells were thawed the same day ...
-
bioRxiv - Immunology 2022Quote: ... were transfected with pHR SIN including cloned transgene and packaging vectors pMD2.G and pCMVdR8.91 using TransIT-Lenti Transfection Reagent (Mirus #MIR6603). On the day of transfection ...
-
bioRxiv - Cancer Biology 2020Quote: ... and pMD2.G (VSVG envelope vector) vectors were transfected into HEK293T cells in 94 mm2 dish using TransIT-LT1 Transfection Reagent (Mirus). The supernatants containing virus particles was collected at 72 hours after transfection ...
-
bioRxiv - Genomics 2023Quote: ... 4ug of packing plasmids psPAX.2 and 2ug of the envelope vector pMD2.G diluted in OptiMEM medium and Trans-Ltl transfection reagent (Mirus). For the generation of gRNA lentivirus ...
-
bioRxiv - Cell Biology 2023Quote: HEK293T cells were transfected with retroviral packaging plasmids (pVSV-G, pBS-CMV-gagpol) and pMRX-IP-HaloTag7-LC3 using TransIT-LT1 (Mirus). The viral supernatants were collected 72 hr after transfection followed by virus concentration using Lenti-X concentrator (TaKaRa) ...
-
bioRxiv - Microbiology 2024Quote: ... HIV-1 ΔENV pseudotyped with VSV-G were generated by transient transfection of HEK 293T cells using TransIT-LT1 (Mirus). For HIV-1 ΔENV ΔVpr with Vpr WT packaged in trans (HIV-1ΔVpr +Vpr WT) ...
-
bioRxiv - Molecular Biology 2024Quote: ... constructs with inserted genes were co-transfected with vectors expressing lentiviral gag/pol (psPAX2) and vsvg (pMD2.G) genes in HEK293T cells to produce lentiviruses by TransIT-LT1 transfection reagent (Mirus). Supernatants containing virus particles were collected at 48 hours and 72 hours following transfection ...
-
bioRxiv - Developmental Biology 2023Quote: ... The dCas9-KRAB-mCherry plasmid was mixed with the pCMV_ΔR8.91 and pMD2.G packaging vectors at a ratio of 9:8:1 with TransIT®-Lenti Transfection Reagent (Mirus) in optiMEM (Gibco ...
-
bioRxiv - Biophysics 2024Quote: ... or 0.4 μg dRfxCas13d-EGFP plus 0.6 μg XIST-targeting RfxCas13d crRNA plasmid using 5 μl TransIT-293 transfection reagent (Mirus Bio). After transfection ...
-
bioRxiv - Cancer Biology 2024Quote: ... HEK293T cells were transfected with the packaging vectors pCMV-dR8.91 and pMD2-G and the sgRNA library or dCas9-BFP-KRAB transfer vector using LT1 transfection reagent (Mirus MIR2300). Lentivirus-containing media was harvested after 48-72 hrs and filtered before use in downstream applications.
-
bioRxiv - Microbiology 2022Quote: ... cells were transfected with 1 µg of DDX60 wt or mutant constructs using TransIT®-LT1 Transfection Reagent (Mirus Bio MIR 2300) at a 1:3 (µg to µl ...
-
bioRxiv - Cancer Biology 2024Quote: ... Cells were transfected 24 hours after plating with 1.0 µg D2HGlo constructs and 3.0 µL Trans-IT®-LT1 transfection reagent (Mirus Bio, Madison, WI). The cells were allowed to express for 48 hours before fluorescence imaging.
-
bioRxiv - Biochemistry 2021Quote: ... and 7 μl of VSV-G pseudovirus in 500 μl of 1X opti-MEM reduced serum free media using TransIT-Pro® as a transfection reagent (Mirus). After 4 hr of transfection ...
-
bioRxiv - Genomics 2021Quote: ... and packaging plasmids for expression of HIV-1 gag/pol and rev (+/-tat) and VSV-G envelope protein using either TransIT®-LT1 Transfection Reagent (Mirus) or Polyethylenimine (Polysciences ...
-
bioRxiv - Microbiology 2023Quote: ... were cotransfected with 1 μg psPAX2-IN/HiBiT,23 1 μg pWPI-Luc2,23 and 500 ng plasmids expressing parental S or its derivatives using TransIT-293 transfection reagent (Mirus, Cat# MIR2704) according to the manufacturer’s protocol ...
-
bioRxiv - Molecular Biology 2023Quote: ... pCW57.1-HLTF vectors were packaged into lentivirus particles using the 2nd generation lentiviral packaging system (pMD2.G & psPAX2) in HEK293T cells using TransIT®-LT1 Transfection Reagent (Mirus). Virus-containing media was harvested 24 & 48h post-transfection and filtered through 0.45 μm PES membrane syringe filter to eliminate packaging cells ...
-
bioRxiv - Genomics 2022Quote: Lentivirus was generated by transfecting HEK239T cells with the transfer plasmid and four packaging plasmids (for expression of VSV-G, Gag/Pol, Rev, and Tat) using TransIT-LT1 Transfection Reagent (Mirus Bio). Viral supernatant was harvested two days after transfection and filtered through 0.44 µm PES filters and/or frozen at –80 °C prior to transduction.
-
bioRxiv - Cell Biology 2023Quote: ... Lentiviral vector was co-transfected with pSPAX2 and pMD2.G plasmids (gifts from Dr. Julius lab) to HEK293T by TransIT-Lenti transfection reagent (Mirus Bio). The lentivirus was produced in DMEM containing 10% FBS and 1% BSA and then concentrated by the Lenti-X concentrator (Takara Bio).
-
bioRxiv - Genetics 2024Quote: ... were co-transfected with the pCMV-dR8.91 packaging and pMD2.G envelope plasmids into the packaging HEK293T cells (AVAM761) using the TransIT-293 Transfection Reagent (Mirus Bio). Following daily media changes (DMEM ...
-
bioRxiv - Microbiology 2021Quote: ... cells were transfected with 3 µg of either each GPC expression plasmid or pCAGGS empty vector using TransIT LT-1 reagent (Mirus, Madison, WI, USA), according to the manufacturer’s instructions ...
-
bioRxiv - Microbiology 2021Quote: ... Cells were then transiently transfected 1 µg pTA-Luc-NL4-3 and different amounts of pEGFP-SF2 using TransIT®-LT1 transfection reagent (Mirus Bio LLC) according to the manufacturer’s instructions ...
-
bioRxiv - Cell Biology 2020Quote: ... 1.5 µg plasmid DNA was diluted in 500 µl serum-free RPMI 1640 and supplemented with 12 µl TransIT-LT1 Transfection Reagent (731-0027, Mirus Bio LLC). After 15 min incubation at room temperature ...
-
bioRxiv - Cancer Biology 2020Quote: ... Lentiviral vectors were incubated with 3rd generation viral packaging vectors (pCMV-VSV-G, pMDLg/pRRE, pRSV-Rev) and LT-1 (Mirus, MIR-2305) in OPTI-MEM media for 30 minutes as described by the provider’s instructions ...
-
bioRxiv - Cell Biology 2021Quote: Lentivirus is produced in HEK293t cells by transfection of lentiviral plasmid and packaging plasmids pMD2.G and psPAX2 using TransIT-VirusGEN® Transfection Reagent (Mirus, MIR6705). After two days of transfection ...
-
bioRxiv - Microbiology 2024Quote: ... pVSV-G and either pLRGatewayIRESeFYP-Vpr/Vpx or pLRGatewayIRESmApple-Vpr/Vpx at a ratio 1:1:1 with TransIT-lenti (Mirus, MIR 6603). To generate HIV vectors to make stable cell lines ...
-
bioRxiv - Cell Biology 2023Quote: ... the packaging cell line HEK293-FT was transfected with pTCP-MPsfGFP together with pSAX2 (12260 Adgene) and pCMV-VSV-G (8454 Adgene) plasmids using the kit TransIT®-293 Transfection Reagent (Mirus Bio) and following manufacturer’s instructions ...