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Citations for Mirus Bio :
101 - 150 of 206 citations for Sulfur Free Cobalt Metallo Organic Standard Co @ 5000 µg g in Hydrocarbon Oil since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2020Quote: Lentivirus was generated by transfecting HEK39T cells with standard 4th generation packaging vectors using TransIT-LT1 Transfection Reagent (Mirus Bio). Media was changed 10 hours post-transfection ...
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bioRxiv - Cancer Biology 2023Quote: ... Lentiviral particles were produced by transfecting HEK293T cells with standard packaging vectors using the TransIT-Lenti Trasfection Reagent (6605, Mirus). CH157-MN cells were stably transduced with lentiviral particles to generate CH-157MN NOTCH3ICD or empty pLVX vector (EV ...
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bioRxiv - Cell Biology 2024Quote: ... Generation of lentivirus was performed by transfecting HEK293T cells with standard delta VPR and VSVG packaging vectors paired with TransIT-LTI Transfection Reagent (MIR2305, Mirus). Lentivirus was harvested 72 hrs following transfection and frozen at -80°C.
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bioRxiv - Cancer Biology 2021Quote: ... d8.9 packaging vector and VSV-G envelope vector mixed with TransIT-293 (Mirus Bio). Virus containing supernatant was collected at 36 ...
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bioRxiv - Immunology 2022Quote: ... 0.4µg pCMV-VSV-G and 1.6µg of viral genomic DNA with TransIT-293 (Mirus) and left O/N ...
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bioRxiv - Cancer Biology 2024Quote: ... d8.9 packaging vector and VSV-G envelope vector mixed with TransIT-293 (Mirus Bio). Virus-containing supernatant was collected 48 and 72 hours after transfection ...
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bioRxiv - Immunology 2021Quote: ... the RP172 plasmids containing the CHST genes were co-transfected with RP18 and RP19 helper plasmids into HEK293T cells after incubating with TransIT®-LT1 transfection reagent (Mirus Bio). After 3 days ...
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bioRxiv - Microbiology 2020Quote: ... Monolayers of BHK-T7 cells at ~50% confluency in 6-well plates were co-transfected using TransIT-LT1 transfection reagent (Mirus Bio LLC) with 0.8 μg of each of ten plasmid constructs representing the T1L or T3DI reovirus genome ...
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bioRxiv - Neuroscience 2023Quote: ... 3 µg of each sgRNA plasmid and 3 µg of dCas9-KRAB-MeCP2 plasmid were co-nucleofected into 1 × 106 mHypoA cells resuspended in a 1:1 mixture of Ingenio Electroporation reagent (Mirus Bio 50111) and OptiMEM (Gibco 31985062) ...
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bioRxiv - Cell Biology 2023Quote: ... HeLa cells were transfected with 25 nM scramble or PLIN 5 siRNA (Dharmacon, Lafayette, CO, USA) using TransIT-TKO (Mirus Bio LLC) according to manufacturer’s instructions.
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bioRxiv - Microbiology 2020Quote: Infectious vaccine viruses were generated from plasmid constructs by transfection into BHK-21J cells using standard protocols (TransIT-LT1, Mirus Bio). The supernatant was harvested four days post-transfection when most of the cells showed signs of CPE ...
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bioRxiv - Biophysics 2024Quote: ... Lentiviral particles containing each plasmid were produced by transfecting standard packaging vectors along with the plasmids into HEK293T cells using TransIT-LT1 Transfection Reagent (Mirus, MIR2306). Media was changed at 24 hours post-transfection ...
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bioRxiv - Cell Biology 2021Quote: ... 5 µg pre-miR-181a-1 were labeled with cy3 using Nucleic Acid Labeling Kit (Mirus) following the manufacturer’s instructions.
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bioRxiv - Cell Biology 2021Quote: ... We generated lentivirus of the resulting constructs by co-transfecting them with 3rd generation lentiviral packaging plasmids with TransIT-Lenti Transfection Reagent (Mirus cat. no. MIR6606) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2020Quote: ... HEK293T cells were transfected with sgRNA library lentivirus vectors and standard packaging vectors (VSVG and psPAX2) using TransIT-LTI transfection reagent (Mirus, Cat# 2306) following previously published protocols.17 Supernatant was harvested 72 h after transfection and filtered through a 0.45 μm SFCA filter ...
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bioRxiv - Molecular Biology 2022Quote: ... Lentivirus was produced by transfecting HEK293T cells with standard packaging vectors using the TransIT-LT1 Transfection Reagent (MIR 2306; Mirus Bio LLC). Viral supernatant was collected 48–72 h after transfection ...
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bioRxiv - Cell Biology 2024Quote: Lentivirus was produced by transfecting HEK293T with pLVX lentiviral plasmid containing RUSH constructs and standard packaging vectors using TransIT-LTI Transfection Reagent (Mirus, MIR 2306). Viral particles in the supernatant was harvested at 2 days after transfection and stored frozen at -80°C until used for infection.
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bioRxiv - Cell Biology 2021Quote: ... Each dish was transfected using the TransIT-LT1 Transfection reagent (Mirus, MIR 2305, 3µL/µg of DNA) with 4.8μg psPAX2 ...
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bioRxiv - Molecular Biology 2023Quote: ... Strains containing selectable markers were cultured on medium with 50 µg/mL hygromycin (Hygromycin B Solution, Mirus) or 25 µg/mL kanamycin (GoldBio ...
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bioRxiv - Microbiology 2023Quote: ... All transfections used 2.49 µg plasmid DNA with 6.25 µL TransIT LT1 transfection reagent (Mirus, Madison, WI) in 250 µL Opti-MEM (Gibco) ...
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bioRxiv - Cell Biology 2024Quote: ... and 1.5 µg of a pLJM1 or pLIX403 plasmid using the TransIT-LT1 Transfection Reagent (Mirus Bio) as per the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2020Quote: ... pCMVΔR8.91 and pMD2VSV-G envelope plasmids into HEK293T cells using TransIT-LTI transfection reagent (Mirus Bio). Virus particles collected at 48 hours post-transfection were passed through a 0.45 μm filter and added to SVOG3e cells at a M.O.I ...
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bioRxiv - Molecular Biology 2024Quote: ... 3.75 μg of psPAX2 and 1.25 μg pMD2.G vectors using the TransIT-VirusGen (Mirus Bio) transfection reagent diluted in Opti-MEM medium (Gibco) ...
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bioRxiv - Evolutionary Biology 2020Quote: ... Cell-free viruses were produced by transfection of HEK293 cells with pNL4-3 using TransIT-LT1 (Mirus) or Fugene HD (Roche ...
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bioRxiv - Biophysics 2024Quote: ... Transfection mixtures were prepared in serum-free DMEM containing 30 µL of TransIT-LT1 reagent (Mirus Bio). Media containing transfection mixture was replaced after six hours ...
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bioRxiv - Genomics 2021Quote: ... cells were transfected with 750 ng plasmid and selected for with 250 µg/mL hygromycin B (Mirus Bio) for 14 days ...
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bioRxiv - Microbiology 2023Quote: ... 1 µg of PCR product was fluorescently labeled using the Label IT-Cy5 Nucleic Acid Labeling Kit (Mirus) following the manufacturer’s instructions ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Cells were transfected with 7.5 µg (total amount) of indicated plasmid(s) using TransIT-2020 transfection reagent (per the manufacturer’s recommendation, Mirus). Following 24–36 h incubation period ...
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bioRxiv - Microbiology 2022Quote: ... Cells were transfected with 7.7 µg of a plasmid expressing HA-tagged M polyprotein using TransIT-LT1 (Mirus) and after 6 h the cells were treated with either DMSO or 1 µM ponasterone A (Pon A) ...
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bioRxiv - Cell Biology 2023Quote: ... cells were transfected with 10 µg of DNA at 70-80% cell density using TransIT-LT1 (Mirus, 2300) for 293T cells and at 50-60% cell density with Lipofectamine 3000 (ThermoFisher Scientific ...
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bioRxiv - Molecular Biology 2023Quote: ... A transfection mix was prepared by adding 2.5 µg plasmid and 7.5 µl transfection reagent TransIT-LT1 (Mirus) in 250 µl OptiMEM medium ...
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bioRxiv - Cell Biology 2024Quote: ... 0.5 µg plasmid containing the appropriate transgene were used for transfection using TransIT-293 transfection reagent (Mirus Bio) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2024Quote: ... and 1.5 µg of the sgRNA plasmid were cotransfected into the cell line using TransIT transfection reagent (Mirus) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2022Quote: ... HEK293T cells were co-transfected with a lentiviral sgRNA expression plasmid and the packaging vectors pCMVΔ8.91 and pMD VSV-G (1:0.7:0.3 mix) using TransIT-293 (Mirus Bio). HEK293T media was collected 48 h post-transfection ...
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bioRxiv - Cell Biology 2024Quote: ... HEK- 293T cells were co-transfected with a lentiviral expression construct and the packaging vectors pCMVΔ8.91 and pMD VSV-G at a ratio of 1:0.7:0.3 using TransIT-293 (Mirus Bio) as per the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2021Quote: ... or 0.25 µg KDELR-GFP and 0.5 µg pcDNA3.1 (− ligand) diluted in 100µl Optimem and 3 µl Mirus LT1 (Mirus Bio LLC). After a further 18 h ...
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bioRxiv - Microbiology 2022Quote: ... Cells were transfected with 7.7 µg of a plasmid (split evenly by mass between the plasmids indicated) using TransIT-LT1 (Mirus) and after 18 h the cells were mock-treated (refreshed media ...
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bioRxiv - Biophysics 2023Quote: ... the cells were plated in a 12.5 cm2 dish and were transfected at 60% confluency with 0.8 µg DNA using TransIT-293 (Mirus, MIR2700). We used a GFP-tagged and C-terminally truncated human HCN1 construct which was cloned into a pEG plasmid as previously reported by Lou & MacKinnon (2017) ...
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bioRxiv - Cancer Biology 2023Quote: ... were done by mixing 2.5 µg of plasmid DNA with 7.5 µl of TransIT-X2 reagent (Mirus, Madison, WI). The mixture was added to 105 CAF or PDAC cells cultured in serum-free and antibiotic-free media overnight.
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bioRxiv - Zoology 2022Quote: ... Four µg of either plasmid were transfected into 6 × 105 C6/36 cells using TransIT-2020 transfection reagent (Mirus) for 4 h ...
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bioRxiv - Cell Biology 2023Quote: ... and 1.2 µg cytomegalovirus 8.91 vector were mixed and prepared for transfection using TransIT-293 transfection reagent (Mirus Bio) per the manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: LMH cells were seeded into 6-well plates at a density of 8.0×10⁵ and transfected with 2.5 µg of GFP-tagged cIL-4 or GFP-tagged vIL-4 using TransIT (Mirus) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2024Quote: ... and 1.2 µg cytomegalovirus 8.91 vector were mixed and prepared for transfection using TransIT-293 transfection reagent (Mirus Bio) per the manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: ... 2.5 µg of IpaH effector encoding plasmids were used to transfect HEK293T using the TransIT 293 transfection reagent (Mirus). 24 hours after transfection ...
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bioRxiv - Cancer Biology 2020Quote: ... 7.5ug PsPax2 and 2.5ug VSV-G using TransIT-LT1 transfection reagent via manufacturer instructions (Mirus; Cat#: MIR 2305). FUGW (Addgene ...
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bioRxiv - Synthetic Biology 2024Quote: ... 165 ng of packaging vector pMD2-G were combined into 7.5uL of TransIT-LT1 (Mirus Bio, MIR 2300) in 300uL Opti-MEM (Gibco) ...
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bioRxiv - Neuroscience 2021Quote: ... Cells were transfected at ∼60% confluency with 2.5 µg of the appropriate DNA construct using the TransIT-LT1 Transfection Reagent (Mirus; MIR2300) and cultured for an additional 2 days ...
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bioRxiv - Molecular Biology 2020Quote: ... 0.02 µg of each mRNA reporter per well was transfected the next day using the TransIT-mRNA Transfection Kit (Mirus) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2020Quote: BHK-21 cells were transfected in 25 cm2 flasks with 8 µg per flask of infectious clone-derived RNA using TransIT transfection reagent (Mirus) as described previously (32) ...
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bioRxiv - Microbiology 2020Quote: ... were transfected with 2·5 µg serine protease expression plasmid or empty vector using TransIT-X2 Dynamic Delivery System (Mirus). One day later ...