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Citations for Anaspec :
401 - 450 of 558 citations since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: ... KVPRNQDWL (AnaSpec, CliniSciences) at 100ng/mL and IL-2 (100IU/mL ...
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bioRxiv - Biophysics 2021Quote: ... Fluorescent labeling of GRNs was performed using HiLyte™ Fluor 405 succinimidyl ester (Anaspec) or HiLyte™ Fluor 647 succinimidyl ester for FRAP studies on GRNs ...
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bioRxiv - Biophysics 2021Quote: ... Fluorescent labeling of PrLD was performed using HiLyte™ Fluor 647 succinimidyl ester (Anaspec) using a similar protocol as described above for GRNs.
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bioRxiv - Pathology 2021Quote: ... The membrane potential of HEK293 cells was examined by loading the cells for 15 min with one of the two voltage sensitive dyes DiBAC4(3) (Bis-(1,3-Dibutylbarbituric Acid)Trimethine Oxonol) (AnaSpec, Fremont, CA, USA) or FluoVolt™ (ThermoFisher Scientific ...
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bioRxiv - Molecular Biology 2021Quote: ... qPCR was performed using Sybr Green master mix (Takyon, AnaSpec Inc.) using the primers listed in Table S6 on a C1000 Touch Thermocycler (BioRad) ...
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bioRxiv - Neuroscience 2021Quote: ... and rabbit anti-P2Y12 (AnaSpec, AS-55043A, 1:1000) were used for immunostaining ...
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bioRxiv - Biophysics 2021Quote: ... The voltage-sensing dye DiSC3 (Anaspec, Fremont CA) was added (1 μM final concentration from a 1 mM stock in DMSO ...
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bioRxiv - Cell Biology 2021Quote: ... human complement 5a receptor 1 (C5aR1) with C5aR-agonist (AnaSpec AS65121, in measuring buffer), human muscarinic 1,2,3,4 ...
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bioRxiv - Microbiology 2021Quote: ... Both antibiotics were used as positive controls as they were commonly used for treating BK.1,4,62 The full-length peptides were commercially produced by Anaspec (Cambridge, UK; for LL-37) and PeproTech (London ...
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bioRxiv - Microbiology 2021Quote: ... followed by the addition of 25 μL of assay buffer containing substrate (FAM-SAVLQ/SG-QXL®520, AnaSpec, Fremont, CA). The substrate was derived from the cleavage sites on the viral polyproteins of SARS-CoV ...
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bioRxiv - Neuroscience 2021Quote: ... or FAM-Aβ1-42 (Anaspec) was dissolved in hexafluoro-2-propanol (HFIP ...
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bioRxiv - Neuroscience 2021Quote: ... and P2RY12 (1:300, AnaSpec # AS-55043A). Sections were then washed thoroughly to remove excess antibodies and treated with fluorescently tagged secondary antibodies ...
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bioRxiv - Neuroscience 2021Quote: ... small peptide biomarker which is a substrate for MMP2/9 (A580, MMP Substrate 1, AnaSpec).
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bioRxiv - Neuroscience 2021Quote: ... the sections were incubated for 30 minutes in ACSF with A580 MMP Substrate 1 (1 μg/ml, Anaspec) at RT ...
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bioRxiv - Cell Biology 2021Quote: ... HiLyte 488 (AnaSpec; Fremont CA) or Biotin-LC-NHS (Thermo Fisher Scientific) ...
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bioRxiv - Neuroscience 2021Quote: ... Lyophilized Hilyte488-tagged amyloid-β42 (Aβ42-Hilyte488, Anaspec) was diluted to 100μM and sonicated for 10min ...
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bioRxiv - Microbiology 2021Quote: ... followed by the addition of Hoechst 33258 (AnaSpec) at a final concentration of 0.09 mM diluted in water (15 min at 37°C) ...
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bioRxiv - Neuroscience 2021Quote: ... followed by incubation overnight at 4°C with rat anti-Mer (1:1000, eBioscience, cat# 14-5751-82) and rabbit anti-P2RY12 (1:2000, AnaSpec, cat# AS-55043A) in 2% NGS and 2% NDS in PBS-T ...
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bioRxiv - Microbiology 2021Quote: ... and exposed for 3h to 500nM or 5μM biotinylated CGRP (AnaSpec) in 100μl RPMI medium added to the apical side ...
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bioRxiv - Neuroscience 2021Quote: ... One milligram of Aβ (Anaspec) was solubilized at 1 mg/mL in 1 mL of 50 mM sodium tetraborate ...
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bioRxiv - Cancer Biology 2021Quote: ... post-transduction approximately 2×105 A2058 cells in 100µl warm PBS were transferred into tube and stained with 10 µg/ml of JC-1 dye (AnaSpec). An additional 100µl from control cells were treated with 200 µM FCCP ...
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bioRxiv - Cell Biology 2021Quote: ... splenocytes were isolated and stimulated with 10nM OVA257-264 peptide (AnaSpec, Fremont, CA, USA) in 10% RPMI (RPMI 1640 plus 10% fetal bovine serum (Fisher) ...
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bioRxiv - Bioengineering 2021Quote: ... flg22 (AnaSpec AS-62633), purified native PcGCE ...
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bioRxiv - Biophysics 2021Quote: The commercially available amyloid Beta 40 (Aβ 40) was purchased from AnaSpec, inc ...
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bioRxiv - Developmental Biology 2021Quote: ... the GLP-1 receptor agonist exendin-4 (10 nM, Anaspec (Fremont CA)) or the KATP-channel opener diazoxide (200 μM ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... UIVT3 (HiLyte Fluor488™-ESATLQSGLRKAK-QXL520™-NH2) (Anaspec) by the enzymes was monitored over time ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: The ThT fluorescence assay was performed using the SensoLyte Thioflavin T β-Amyloid Aggregation Kit (ANASPEC, Fremont, CA, USA). Aβ and ThT solutions at final concentrations of 50 μM and 200 μM ...
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bioRxiv - Neuroscience 2021Quote: ... HiLyte™ Fluor 488-labeled amyloid β peptide 25-35 (Anaspec, AS-633308) was prepared according to the manufacturer’s protocol ...
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bioRxiv - Biophysics 2021Quote: ... actin was labeled at Cys374 with erythrosin-5’-iodoacetamide (ErIA; AnaSpec, Fremont, CA)(11,14) ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: Substrate was prepared by reconstituting MAPL-DNP (Anaspec) in DMSO to make 1 mM stock with gentle mixing by inversion to ensure dissolution ...
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bioRxiv - Cancer Biology 2021Quote: ... αSMA 1:100 (AS-29553, Anaspec), CD31 1:100 (MEC13.1 ...
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bioRxiv - Immunology 2021Quote: ... Cathepsin L (CtsL) activity was measured using the SensoLyte ® Rh110 Cathepsin L Assay Kit (AS-72217, Anaspec) per the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2021Quote: ... against P2Y12-receptor was from AnaSpec (Cat. No. AS-55043A), against Iba1 was from Synaptic System (Cat ...
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bioRxiv - Neuroscience 2021Quote: ... they were incubated with rabbit anti-P2Y12R (1:500, #55043AS AnaSpec) alone or mixed with mouse anti-GFAP (1:1000 ...
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bioRxiv - Neuroscience 2021Quote: ... The following primary antibodies were used: rabbit anti-P2Y12R (1:500, #55043AS AnaSpec), chicken anti-GFP-tag (1:500 ...
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bioRxiv - Neuroscience 2021Quote: ... Sections were then incubated in different mixtures of primary antibodies: rabbit anti-P2Y12R (1:500, #55043AS AnaSpec), chicken anti-GFAP (1:500 ...
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bioRxiv - Microbiology 2021Quote: Antimicrobial peptide susceptibility Human neutrophil defensin-1 (hNP-1) (AnaSpec Incorporated, California, USA) and LL-37 (Sigma ...
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bioRxiv - Biochemistry 2021Quote: ... 0.2 μM NRMT1 or 0.2 μM NRMT1+NRMT2 was incubated in wells on a 96-well plate with 40 μM of synthetic peptide substrate corresponding to the first 15 N-terminal amino acids of ZHX2 (minus the initiating methionine) (AnaSpec, Fremont, CA) in the presence of 40 μM S-adenosyl methionine (SAM) ...
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bioRxiv - Neuroscience 2021Quote: IDE activity was measure in cortical homogenates posttreatment for1 hour using the SensoLyte IDE Activity Assay Kit (AnaSpec, Inc: Cat. # AS-72231) according to manufacturer’s instructions ...
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bioRxiv - Biochemistry 2021Quote: ... a 20 μM concentration of substrate [Ac-DE-Dap(QXL520)-EE-Abu-γ-[COO]AS-C(5-FAMsp)-NH2] (AnaSpec) was serially diluted into assay buffer [50 mM Tris ...
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bioRxiv - Biochemistry 2021Quote: ... The proteolytic reaction was initiated by the injection of 5 μL of HCV NS3/4A protease substrate (AnaSpec), to a final concentration of 200 nM and kinetically monitored using a Perkin Elmer EnVision plate reader (excitation at 485 nm ...
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bioRxiv - Biochemistry 2021Quote: ... and 1 mM 5-FAM-Lysine (Anaspec), prepared from a 20 mM stock in DMSO ...
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bioRxiv - Microbiology 2021Quote: ... HOBt was purchased from AnaSpec (Fremont, CA). Fmoc-amino acids were purchased from Matrix Innovation (Quebec ...
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bioRxiv - Microbiology 2021Quote: Antimicrobial peptide susceptibility Human neutrophil defensin-1 (hNP-1) (AnaSpec Incorporated, California, USA) and LL-37 (Sigma ...
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bioRxiv - Biochemistry 2021Quote: ... the substrate FAM-QRVRRAVGIDK-TAMRA (AnaSpec # 808143 ...
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bioRxiv - Biochemistry 2021Quote: Lyophilized Aβ powder was purchased from Anaspec. The samples were disentangled via ammonium hydroxide treatment.29 100 µg aliquots of each peptide were dissolved in 50 µL of 1% ammonium hydroxide solution (w/v ...
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bioRxiv - Biophysics 2021Quote: The preparation of α-Synuclein aggregates is followed from our previous work from Zhou and Kurouski.15 α-Syn (AnaSpec, CA, USA) was dissolved to the final concentration of 50 μM ...
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bioRxiv - Cancer Biology 2021Quote: ... and then eluted by 50 μM 3X-HA peptide (AnaSpec). Each eluate was measured by intact mass LC-MS to determine the percentage of complex with compound adduct.
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bioRxiv - Neuroscience 2021Quote: ... or the Y1 selective agonist [Leu31,Pro34]-NPY (Anaspec, USA) by pressure ejection with a pipette positioned close (∼50 µm ...
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bioRxiv - Neuroscience 2021Quote: ... anti-P2RY12 (1:1000; AnaSpec, Fremont, CA), anti-GFAP (1:500 ...