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Citations for Anaspec :
151 - 200 of 558 citations since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2023Quote: ... followed by HRP-conjugated secondary antibody (Anaspec, 1:2000). Antibodies were dissolved in 1X TBS with 0.05% Tween-20 ...
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bioRxiv - Cancer Biology 2023Quote: ... The kinase reaction was initiated by the addition of ATP (10 µM final) and fluorescein labeled Scrtide peptide (AnaSpec, Fremont, CA)(1 µM final) ...
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Antigen Geometry Tunes Mast Cell Signaling Through Distinct FcεRI Aggregation and Structural ChangesbioRxiv - Biophysics 2023Quote: ... custom DF3 peptide synthesized by and purchased from Anaspec (Fremont, CA) and anti-DNP-IgE was affinity-purified from ascites (Covance ...
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bioRxiv - Biophysics 2023Quote: ... Residual native CaM was stripped from SR by incubation with 300 nM of the M13 skeletal muscle myosin light-chain kinase CaM-binding peptide (Anaspec, Fremont, CA, USA), as described previously [35] ...
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bioRxiv - Neuroscience 2023Quote: ... Synthetic human Aβ1-42 peptides (Anaspec) were dissolved in hydroxyfluroisopropanol (HFIP ...
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bioRxiv - Neuroscience 2023Quote: ... anti-P2ry12 (AS-55043A, AnaSpec, 1:1000), anti-Tmem119 (ab209064 ...
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TNFR1/p38αMAPK signaling in Nex+ supraspinal neurons regulates sex-specific chronic neuropathic painbioRxiv - Neuroscience 2023Quote: ... nuclei were stained with Hoechst 33258 (1:10000, Anaspec AS-83219), and then slides were cover-slipped (Fluoro-Gel ...
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bioRxiv - Neuroscience 2023Quote: Commercial Aβ 1-42 monomer (Anaspec, AS-20276) was prepared at 2 μM in saline sodium phosphate EDTA (SSPE ...
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bioRxiv - Neuroscience 2023Quote: ... 40µM PKC substrate peptide (amino acid sequence ERMRPRKRQGSVRRRV; AnaSpec, Fremont, CA), 50µM ATP ...
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bioRxiv - Neuroscience 2023Quote: ... The ψεRACK peptide (HDAPIGYD) was obtained from AnaSpec Inc ...
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bioRxiv - Microbiology 2023Quote: ... 1 μM Jagged 1 (Anaspec), 10 nM Y-27632 (Cayman Chemical Company) ...
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bioRxiv - Neuroscience 2023Quote: ... Aβ3(pE)-42 (Anaspec, Cat.: #AS-29907), and FAM-Aβ3(pE)-42 (Eurogentec ...
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bioRxiv - Neuroscience 2023Quote: ADAM10 activity was measured via fluorometric FRET assay following manufacturer’s instructions (AS-72226, Sensolyte 520, Anaspec) and as previously described41 ...
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bioRxiv - Immunology 2023Quote: ... 4μM of OVA (OVA 257-264, Anaspec Inc., Cat#AS-60193-5) or gp33 (gp33-41 ...
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bioRxiv - Microbiology 2023Quote: Antimicrobial peptide susceptibility Human neutrophil defensin-1 (hNP-1) (AnaSpec Incorporated, California, USA) and LL-37 (Sigma ...
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bioRxiv - Neuroscience 2023Quote: ... for 30 min and incubated with blocking solution (5% normal bovine serum in PBST) for 1 h followed by rabbit anti-P2Y12 antibody (AS-55043A, AnaSpec) together with goat anti-Iba1 antibody (ab5076 ...
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bioRxiv - Bioengineering 2023Quote: The (KKEEE)3K targeting peptide was synthesized using standard Fmoc-mediated solid phase peptide synthesis methods on rink Amide resin (Anaspec, Fremont, CA, USA) using an automated benchtop peptide synthesizer (PS3 ...
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bioRxiv - Immunology 2023Quote: ... 1 µL of HiLyte™ Fluor 488-labeled Amyloid-beta (1-42) (Anaspec) prepared by reconstituting 0.1mg in 50 µL of 1% NH4OH ...
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bioRxiv - Immunology 2023Quote: ... Hoechst 33258 (AnaSpec Inc; Freemont, CA) for all the sets and incubate at 37°C for 1 hour [25] ...
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bioRxiv - Physiology 2023Quote: 0.1 mg of lyophilized HiLyte Fluor 488-labeled amyloid-β1-42 (Anaspec AS-60479-01) was dissolved in 50 µL of 1% NH4OH and diluted to 0.5 mg/mL with 1xPBS before aliquoting for storage at −20°C until further use ...
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bioRxiv - Biophysics 2023Quote: ... To label mSA with a thiol-reactive dabcyl quencher (dabcyl-maleimide, AnaSpec), Tyr83 near the biotin-binding pocket in the active subunit was mutated to cysteine ...
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bioRxiv - Developmental Biology 2023Quote: ... rabbit anti-Shha (AnaSpec,, AS-55574, 1:20), rabbit anti-human PROX1 (ReliaTech ...
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bioRxiv - Microbiology 2023Quote: ... pestis KIM6+ was examined using membrane potential sensitive fluorophore 3,3-dipropylthiadicarbocyanine iodide [DiSC3(5)] (Anaspec Inc, USA) (33) ...
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bioRxiv - Neuroscience 2023Quote: MHC II Ea chain (Ea) (52–68) peptide (Anaspec) was reconstituted in sterile distilled H 2O to a final concentrate of 1mG/mL ...
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bioRxiv - Microbiology 2023Quote: ... 0.5 µg LL-37 ml-1 (Anaspec), or both.
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bioRxiv - Immunology 2023Quote: ... Cells were washed and stimulated in the presence of OVA257-264 (1 µg/ml) (Anaspec), recombinant murine IL-2 200 U/ml (Peprotech ...
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bioRxiv - Biochemistry 2023Quote: ... Acrylodan (Anaspec) for peptide substrate labeling was solubilized in acetonitrile with the stock concentration determined by absorbance at 393 nm in methanol (ε393 = 18,483 M−1 cm−1 ...
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bioRxiv - Neuroscience 2023Quote: ... prepared from patient donors) or 200μg recombinant human myelin oligodendrocyte glycoprotein (hMOG 1–125, AS-55158-1000, AnaSpec) emulsified in complete (CFA ...
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bioRxiv - Immunology 2023Quote: ... or LCMV276-286 peptides (Anaspec) in RPMI + 10% FBS ...
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bioRxiv - Bioengineering 2023Quote: ... was determined via analysis of spent culture medium (n = 5/group/time) using a commercially available FRET-based generic MMP activity kit (SensoLyte 520 Generic MMP Activity Kit Fluorimetric; Anaspec). MMP activity is represented as the concentration of MMP cleaved product (5-FAM-Pro-Leu-OH) ...
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bioRxiv - Neuroscience 2023Quote: ... AngIIfluo (at a concentration of 3µmol/L, obtained from Anaspec, CA) was infused at a rate of 2.86µl/g/rat and allowed to circulate for a duration of 30 minutes 68 ...
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bioRxiv - Neuroscience 2023Quote: ... β-Amyloid (Anaspec-AS-60479).
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bioRxiv - Neuroscience 2023Quote: The drugs used in this study included the following: human ACTH 1-24 (AnaSpec, USA), 150μg per animal dissolved in 0.9% saline ...
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bioRxiv - Cell Biology 2023Quote: ... washed again 3X with PBS/1% NDS and lastly with PBS containing Hoechst33342 (AnaSpec, AS-83218) at a 1:1000 dilution ...
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bioRxiv - Cell Biology 2023Quote: ... MOG35-55 4mg/mL in PBS (Anaspec, Fremont, CA), and M ...
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bioRxiv - Cell Biology 2023Quote: Analyses of MMP-2 activity were performed by a fluorescence assay kit (SensoLyte® 520 MMP-2 kit, AnaSpec, San Jose, USA). The fluorescence of the substrate cleavage product was determined at excitation/emission = 490 nm/520 nm with a Perkin-Elmer spectrofluorometer [21,22].
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bioRxiv - Immunology 2023Quote: ... The bone marrow-derived dendritic cells were pulsed with 10µM OTII-peptide (OVA 329-337 peptide; Anaspec # AS-64777) for 2 hours at 37°C ...
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bioRxiv - Immunology 2023Quote: ... pulsed with 10µM GP100 peptide (25-33; EGSRNQDWL; Anaspec AS-64752) for 1h ...
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bioRxiv - Cell Biology 2023Quote: ... the sections were reacted with polyclonal anti-GFP antibody (AnaSpec, Fremont, CA, US) diluted 1:500 in 3% (w/v ...
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bioRxiv - Neuroscience 2023Quote: ... rabbit anti-MMP2 (AS-55111, 1:250; AnaSpec, Fremont, CA, USA), mouse anti-TH (1:1000 ...
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bioRxiv - Neuroscience 2023Quote: ... + 1xPBS + .03% Triton + primary antibodies (chicken anti-Iba1: 1:1000, Synaptic Systems 234 009; rabbit anti-P2RY12: 1:500, Anaspec AS-55043A). After primary antibody incubation ...
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bioRxiv - Cell Biology 2023Quote: ... and a third wash using Hoechst 33342 (AnaSpec, AS-83218) in PBS for 5 minutes ...
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bioRxiv - Immunology 2023Quote: ... primed with 200 μg GP61-80 peptide (AnaSpec). The next day ...
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bioRxiv - Immunology 2023Quote: ... Total anti-MOG IgG was quantified by using SensoLyte Anti-Mouse MOG(1–125) IgG Quantitative ELISA Kit (Anaspec).
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bioRxiv - Neuroscience 2023Quote: ... The SensoLyte Red Glucocerebrosidase (GBA) Activity Assay Kit (AnaSpec, AS-72259) was used to determine enzyme activity in serum and whole tissue lysate samples ...
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bioRxiv - Immunology 2023Quote: ... of the original Wuhan strain of SARS-CoV-2 spike protein were designed from a full-length RBD cDNA sequence (accession # NC_045512.2) and conjugated with keyhole limpet hemocyanin (KLH) (Anaspec, Richmond CA) for immunizations and an unconjugated peptide for screening assays ...
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bioRxiv - Microbiology 2023Quote: ... 0.09 mM Hoechst 33258 (AnaSpec, catalog# AS-83219) stain was added to stain the nucleus for 10 minutes ...
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bioRxiv - Microbiology 2023Quote: ... followed by addition of Hoechst 33258 (AnaSpec) at a final concentration of 90 μM in water (15 min ...
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bioRxiv - Microbiology 2023Quote: ... control mice were inoculated with either PBS or the parental peptide LL-37 (Anaspec).
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bioRxiv - Molecular Biology 2023Quote: ... Hoechst 33342 (AnaSpec, AS-83210) (1:3000) ...