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Citations for Anaspec :
351 - 400 of 558 citations since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2022Quote: ... 2×106 splenocytes were plated in 96-well plates (Fisher, #130188) in 200 μL T-cell media and stimulated with the indicated peptide (SIINFEKL [Anaspec, #AS-60193-1] ...
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bioRxiv - Immunology 2022Quote: BMDMs generated above were pulsed with GP100 (1µg/ml) or LCMV (1µg/ml, negative control) peptides (AnaSpec). Additionally ...
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bioRxiv - Biochemistry 2022Quote: ... 50 nM HAT module was incubated in buffer along with 0-700 mM H3 peptide (amino acids 1-21) (AnaSpec) at 30°C in 384-well plates (Greiner Bio-One ...
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bioRxiv - Cancer Biology 2022Quote: ... Dithiothreitol (DTT) was purchased by AnaSpec. Reagents and solvents were used without further purification.
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bioRxiv - Biophysics 2021Quote: ... the peptides were labeled at their N-terminus with an amine-reactive environmentally sensitive fluorescent reporter, Succinimidyl 6-(N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)amino)hexanoate (NBD-X, SE) (AnaSpec, Fremont, CA) [37] ...
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bioRxiv - Biophysics 2021Quote: ... and magainin-2 were purchased from Anaspec (Fremont, CA). All peptides were 95% or greater purity ...
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bioRxiv - Cancer Biology 2021Quote: ... splenocytes were isolated and stimulated with 10 nM OVA257-264 peptide (AnaSpec, CA, USA) in complete media containing Gibco RPMI 1640 (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2021Quote: ... 1x Pen/Strep and 1x L-Glut) supplemented with LCMV GP33-41 peptide (0.5μg/mL, AnaSpec, cat. AS-61296) or left unstimulated ...
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bioRxiv - Immunology 2021Quote: ... Ex vivo stimulation of T cells was performed with 100ng/ml PMA and 1ug/ml ionomycin or 200mg/ml gp33 peptide (Anaspec) for 4-5 hours in the presence of brefeldin A ...
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bioRxiv - Immunology 2021Quote: ... slides were incubated in blocking at RT for 1 h before overnight incubation at 4°C with the primary rabbit anti-P2Y12 receptor antibody (1:200, AnaSpec #AS-55043A) and labelling for 1 hour with the secondary antibody AF488 goat anti-rabbit ...
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bioRxiv - Microbiology 2021Quote: ... histone H3K9me3 (Anaspec) were used for the experiment ...
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bioRxiv - Molecular Biology 2021Quote: Peptide-pull down was performed with synthetic peptide corresponding to H2A N-terminal (aa 1-23) obtained from AnaSpec. 1μg of peptide was incubated with 1μg of GST-tagged protein overnight on wheel at 4°C in binding buffer (50 mM Tris–HCl pH 7.5 ...
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bioRxiv - Developmental Biology 2021Quote: ... rabbit anti-BMP2b-Zebrafish (1:50, Anaspec, AS-55708), mouse anti-NeuroD1 (1:500 ...
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bioRxiv - Immunology 2021Quote: ... and 10 μM LCMV gp33-41 peptide (AnaSpec #AS-61296). CD8 T cells from spleens and lymph nodes of either P14 or FSF-Tim-3/E8i-Cre/P14 mice were purified by magnetic separation using a naïve CD8+ T cell isolation kit (Miltenyi Biotec #130-104-075 ...
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bioRxiv - Neuroscience 2021Quote: ... 0.05% Brij-35) and 1 µM of 520 MMP FRET substrate III (Cat. No. 60570-01; AnaSpec, San Jose, CA) was added ...
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bioRxiv - Neuroscience 2021Quote: Amyloid-beta (Aβ1-42) (AnaSpec; Fremont, CA) was prepared using two different methods as indicated in the figure legends (Methods A or B) ...
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bioRxiv - Neuroscience 2021Quote: ... Amyloid-beta (Aβ1-42) peptide (AnaSpec; Fremont, CA) was dissolved in Hexafluoro-2-propanol (HFIP ...
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bioRxiv - Neuroscience 2021Quote: ... with a total 200μg MOG35-55 (AnaSpec, AS-60130-1) per mouse was injected subcutaneously into all 4 flanks ...
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bioRxiv - Molecular Biology 2021Quote: ... After the addition of 15 µl of 5× click reaction buffer (7.5 mM sodium ascorbate [Nacalai Tesque], 0.5 mM TBTA [AnaSpec], and 5 mM CuSO4) and 1.5 µl of 10 mM Azide-PEG4-Biotin (Click Chemistry Tools) ...
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bioRxiv - Molecular Biology 2021Quote: ... or dabcyl C2 maleimide (AnaSpec) via a maleimide-sulfhydryl reaction overnight at 4 °C and then removed free dye by gel filtration chromatography ...
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bioRxiv - Molecular Biology 2021Quote: ... KAAR - K(Ac) – SAPATGG were from AnaSpec, Inc.
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bioRxiv - Immunology 2021Quote: ... together with antigenic peptides (for Pmel-1, gp10025-33; for OT-1, OVA257-264) at 10μg/mouse (peptides were purchased from Anaspec). The control (CON ...
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bioRxiv - Molecular Biology 2021Quote: ... synthetic Aβ1-42 (Anaspec AS-60479-01) was prepared by resuspending the lyophilized protein in hexafluoroisopropanol (HFIP MPBio #151245) ...
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bioRxiv - Molecular Biology 2021Quote: ... Phagocytosis assays were done by incubating BV2 cells with 100 µM HiLyte™ Fluor 488-labeled human Aβ1-42 (Anaspec #AS-60479-01) for three hours in the presence or absence of the hybrid protein at 37°C for Aβ uptake and at 4°C for surface binding assay following Prasad and Rao (2018).
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bioRxiv - Molecular Biology 2021Quote: ... the cells were treated with 1 µm non-fluorescent Aβ1-42 (Anaspec #AS-20276) in the presence or absence of the hybrid protein in phenol red-free and serum-free DMEM ...
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bioRxiv - Biochemistry 2021Quote: Human ACE2 activity was evaluated using SensoLyte® 390 ACE2 Activity Assay Kit (ANASPEC; cat# 72086) according to manufacturer’s protocol ...
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bioRxiv - Immunology 2021Quote: Cathepsin D (CtsD) activity was measured using the SensoLyte ® 520 Cathepsin D Assay Kit (AS-72170, Anaspec) per the manufacturer’s protocol ...
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bioRxiv - Immunology 2021Quote: ... KVPRNQDWL (AnaSpec, CliniSciences) at 1000 ng/mL.
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bioRxiv - Immunology 2021Quote: ... a splenocyte suspension from one OT-I Rag2-/- spleen was stimulated with 10nM Ova257-264 peptide (SIINFEKL, AnaSpec, Cat no. 60193-5-ANA) for 48h and cultured for up to 10 days ...
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bioRxiv - Cell Biology 2021Quote: ... and Aβ-TMR (Aβ1–42-5-tetramethyl rhodamin) suspended in 1,1,1,3,3,3-hexafluoro-2-propanol (AnaSpec). Lyophilized Aβ and Aβ-TMR were resuspended at a concentration of 5 mM in Me2SO and then diluted to a concentration of 100 μM in HEPES buffer ...
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bioRxiv - Microbiology 2021Quote: ... Mca-APK-Dnp (AnaSpec) 45 ...
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bioRxiv - Microbiology 2021Quote: ... FRET peptides were obtained from Anaspec and a peptide sequences are listed in Supplementary Fig S1F ...
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bioRxiv - Cancer Biology 2021Quote: ... Matrix solution was mixed 12:1 (v/v) with an internal calibrant mix containing 0.08 µg/ml Angiotensin I (Anaspec, Seraing, Belgium), 0.04 µg/ml Substance P (Anaspec ...
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bioRxiv - Cancer Biology 2021Quote: ... 0.04 µg/ml Substance P (Anaspec, Seraing, Belgium), 0.15 µg/µl [Glu]-Fibrinopeptide B (Sigma-Aldrich ...
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bioRxiv - Bioengineering 2021Quote: ... 1uM calcein AM (AnaSpec), and 7.5uM propidium iodide (Sigma Aldrich ...
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bioRxiv - Biochemistry 2021Quote: ... and [5F]MP were synthesized by Anaspec Inc ...
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bioRxiv - Biophysics 2021Quote: ... a series of 250 μL injections were used to clean the pepsin column: (1) Fos-choline-12 (Anaspec) in 0.1% TFA ...
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Long-Range Electrostatic Interactions Significantly Modulate the Affinity of Dynein for MicrotubulesbioRxiv - Biophysics 2021Quote: ... We incubated the washed beads with 0.25 mg/mL mouse monoclonal biotinylated anti-His Tag monoclonal antibody (AS-61250-BIOT, Anaspec Inc., Fremont, CA) for 2 hours at 22°C ...
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bioRxiv - Neuroscience 2021Quote: ... rabbit anti-P2Y12 (1:400, Anaspec, AS-55043A), rabbit anti-Iba1 (1:500 ...
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bioRxiv - Neuroscience 2021Quote: ... synthetic Aβ1–42 (AnaSpec) was dissolved in 1,1,1,3,3,3-hexafluoro-2-propanol (Sigma-Aldrich ...
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bioRxiv - Neuroscience 2021Quote: We added 1 mg Aβ peptide (1-42) (Anaspec, CA, USA) into a microtube containing 270 μL of 1,1,1,3,3,3-Hexafluoro-2-propanol (HFIP ...
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bioRxiv - Neuroscience 2021Quote: ... beta-Amyloid (1-42) (Anaspec, AS-60883), Thioflavin S (Sigma T1892) ...
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bioRxiv - Neuroscience 2021Quote: ... The synthetic human Aβ1-42 or Aβ40 peptide (AnaSpec) was used for standard curves for each assay ...
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bioRxiv - Neuroscience 2021Quote: ... rabbit anti-P2Y12R (1:500; #55043AS, AnaSpec). After the incubation with the primaries ...
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bioRxiv - Physiology 2021Quote: ... Di-4-ANEPPS (AnaSpec, Catalog# AS-84723, 1 µM in KH buffer), was perfused to image excitation of the heart ...
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bioRxiv - Physiology 2021Quote: Fluorescently labeled peptides were synthesized by Anaspec. The fluorescence polarization was performed as described previously by us9 ...
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bioRxiv - Biochemistry 2021Quote: ... using the fluorogenic peptide substrates suc-LLVY-amc (AnaSpec, AS-63892), boc-LRR-amc (AdipoGen ...
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bioRxiv - Biochemistry 2021Quote: Synthetic Aβ(1-40) was purchased from AnaSpec Inc ...
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bioRxiv - Cell Biology 2021Quote: ... and 1mM Jagged-1 (AnaSpec AS-61298). Organoids were maintained at 37°C ...
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bioRxiv - Microbiology 2021Quote: ... pneumoniae cells were induced to competence by the addition of synthetic competence stimulatory peptide 1 (CSP-1; Anaspec, Inc.). Markerless deletions and replacements of target genes were constructed using the kanR-rpsL+ (Janus cassette ...