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Citations for abcam :
4851 - 4900 of 5060 citations for Recombinant Mouse Gfra1 His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... 1:300 dilution of mouse anti-PfDHODH #3 (a kind gift from Margeret Phillips, University of Texas Southwestern) or 1/5000 mouse anti-PfAldolase-HRP (Abcam, Cat #ab38905). The next day ...
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bioRxiv - Microbiology 2021Quote: ... horseradish peroxidase (HRP)-conjugated goat anti-rabbit IgG and HRP-conjugated goat anti-mouse IgG were purchased from Abcam (Cambridge, MA, USA). Purified ZIKV-E protein from Escherichia coli was purchased from MyBioSource (San Diego ...
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bioRxiv - Microbiology 2021Quote: ... and then stained for 2 till 16 hours with rabbit anti-IFITM3 polyclonal antibody (1:200 dilution from proteintech catalogue number 11714-1-AP) and mouse anti-LAMP1 monoclonal antibody (1:100 dilution from Abcam [H4A3] (ab25630)) ...
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bioRxiv - Microbiology 2020Quote: ... the membrane was washed three times with Tris-buffered saline containing 0.01% Tween 20 and incubated for one hour with secondary mouse HRP-conjugated antibody (Abcam Inc., Cambridge, MA). All incubations and wash steps were performed at room temperature unless otherwise stated ...
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bioRxiv - Neuroscience 2021Quote: ... diluted in PBS plus 2% NBCS and 0.3% Triton X-100: mouse anti-Glutamate decarboxylase (65 kD isoform, GAD65; 1:1500; ab26113; Abcam, Cambridge, United Kingdom), rabbit anti-Calbindin (1:3000 ...
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bioRxiv - Bioengineering 2021Quote: ... The sections were incubated at room temperature for 1 hour with primary antibodies: rabbit monoclonal anti-mouse CD4 antibody (1:1000 dilution, ab183685, Abcam, Cambridge, MA), and rabbit polyclonal anti-mouse CD8 antibody (1:500 dilution ...
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bioRxiv - Neuroscience 2022Quote: ... for 1 hr before incubation with primary antibodies (mouse anti-tyrosine hydroxylase 1:1000, AB152; rabbit anti-mCherry 1:1000, ab167453; chicken anti-GFP 1:1000, ab13970; Abcam, Cambridge, UK) in blocking solution at room temperature on a shaker for 18 hr ...
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bioRxiv - Physiology 2022Quote: ... 4 µm serial sections of paraffin-embedded slides were incubated with rat anti-mouse CD34 (MEC 14.7, monoclonal, Abcam, Cambridge, United Kingdom) at a 1:200 dilution overnight at 4° ...
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bioRxiv - Cell Biology 2022Quote: ... Coverslips were blocked with PBS containing 0.1% saponin and 1% BSA for 30 min and incubated with primary mouse anti-LAMP1 antibody (Abcam, ab25630, 1:3000) or rabbit anti-CTSD antibody (Abcam ...
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bioRxiv - Genomics 2022Quote: ... Cells were collected and incubated with antibodies against Anti-Mouse Integrin alpha 6 antibody (GoH3) conjugated to PE (1:600) (Abcam, Cat#: ab95703), a 1:200 CD104 antibody conjugated to FITC (Biolegend ...
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bioRxiv - Cell Biology 2019Quote: ... rabbit anti-SP-D (1:1000 - Chemicon, rabitt anti TNFα (1:50 – Hycult) or mouse anti-TLR4 (1:300 Abcam, Maryland, USA). Blots were incubated with a peroxidase-conjugated (HRP ...
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bioRxiv - Plant Biology 2020Quote: ... Membranes were washed 3 times for 15 mins in 1X Tris-buffered saline (TBS) with 0.1% Tween20 (TBST) and incubated with HRP-conjugated goat anti-mouse antibody (abcam, catalog number ab6789). All antibodies were used at a concentration on 1:5000 ...
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bioRxiv - Cancer Biology 2019Quote: ... Whole pancreas protein extracts were isolated and incubated with mouse inflammation/cytokine antibody arrays (40 targets, Abcam, ab133999; 97 targets, Abcam ab169820), according to manufacturer’s instructions ...
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bioRxiv - Animal Behavior and Cognition 2020Quote: ... The rehydrated slides were blocked with 2% horse-serum followed by incubation with primary antibodies (Mouse anti-S-glycoprotein antibody, 1:200 dilution, Millipore, Cat # MAB5676; Rabbit anti-ACE2 antibody, 1:200 dilution, Abcam, Cat # ab272690) for 2 hrs ...
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bioRxiv - Microbiology 2020Quote: ... for 2 h at room temperature and a secondary antibody (1:500; Donkey anti-mouse IgG H&L, Alexa Fluor® 568, Abcam #ab175472) for 1 h at room temperature in the dark ...
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bioRxiv - Bioengineering 2021Quote: ... the channels were incubated at 4 °C for 16 h with the following primary antibodies: anti-mouse laminin MEC5 (rat IgG; Catalog number ab2466; 1:50; Abcam, Cambridge, UK), anti-mouse collagen IV (rabbit IgG ...
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bioRxiv - Cell Biology 2019Quote: Cells were fixed 48 h post transfection with 4% paraformaldehyde (PFA) and co-stained with rabbit anti-HA (1:500) and Mouse anti-CD63 (1:200; Abcam, Cambridge, MA) primary antibodies and with Alexa Fluor 488 anti-rabbit and Alexa Fluor 594 anti-mouse (1:500 ...
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bioRxiv - Cell Biology 2019Quote: ... the cells were stained using the EXPOSE Mouse and Rabbit Specific HRP/DAB Detection IHC kit (Abcam, United States; Cat. no. ab80436). The enzyme expression was visualized with the mouse monoclonal antibody anti-SOD2 (Santa Cruz ...
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bioRxiv - Neuroscience 2019Quote: ... dried skimmed milk in Tris-buffered saline containing 0.1% Tween-20 (TBS-T) and incubated overnight at 4°C with primary antibodies against NDUFS4 (Abcam, mouse, 1:500), NSE (Dako ...
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bioRxiv - Pathology 2020Quote: Formalin-fixed paraffin-embedded tissue sections were subjected to antigen retrieval with EDTA buffer in high pressure heated water bath and staining was performed using either mouse monoclonal ERRα (1:2500, Abcam, Cambridge, MA) or ERRγ (1:400 ...
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bioRxiv - Neuroscience 2021Quote: ... membranes were incubated for 1h with their respective secondary fluorescent antibodies: anti-mouse (1:2,500, IRDye 800, Abcam, Cat# ab216772, RRID: AB_2857338) and anti-rabbit (1:2,500 ...
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bioRxiv - Pathology 2021Quote: ... The RBD-specific IgA titer of BAL samples was next evaluated by ELISA as described above with Goat anti-mouse IgA alpha chain-HRP (Abcam, 1:3000).
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bioRxiv - Genetics 2021Quote: ... embryos were washed 3 times 10 minutes in 1x PBS with 0.1% Triton-X100 and 0.1% BSA and incubated overnight at 4°C with the secondary antibody goat-anti-mouse A568 1:500 (Abcam, antibody ID# ab175473). On the 4th day ...
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bioRxiv - Microbiology 2020Quote: ... 293T cells were fixed and processed for immunostaining against dsRNA (Scicons, 10010500) and visualized with goat anti-mouse IgG H&L (Cy5 ®) (Abcam, ab6563). Images were taken using Nikon Eclipse Ti-E Spinning Disk under 20X and 60X ...
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bioRxiv - Microbiology 2021Quote: ... antibody solutions were diluted in PBS with 0.1% saponin at a 1:10 dilution for mouse α-LAMP-1 (ab25630, Abcam, Cambridge, UK), 1:50 for rabbit α-EEA1-Alexa Fluor 647 (ab196186 ...
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bioRxiv - Immunology 2021Quote: ... Concentration of circulating LPS-binding protein (LBP) was determined in plasma samples (diluted 1:5) using Mouse LBP ELISA Kit (Abcam, Cambridge, MA) according to manufacturer’s instructions ...
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bioRxiv - Cell Biology 2021Quote: ... The pellet was re-suspended with 0.9% of sodium chloride solution and reacted with a rabbit anti-mouse HMGB1 primary antibody for 3 hrs at room temperature (1:350, abcam, Cat. #ab18256), followed by a goat anti-rabbit secondary antibody conjugated with Cy3 for 1hr at room temperature (1:500 ...
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bioRxiv - Microbiology 2021Quote: ... was used for indirect immunofluorescence microscopy at a dilution of 1:300 and mouse monoclonal antibody against nuclear pore complex proteins (Abcam, Cambridge, MA) was used for indirect immunofluorescence microscopy at a dilution of 1:500 ...
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bioRxiv - Cell Biology 2021Quote: ... lungs were incubated with conjugated fluorescent secondary Alexa Fluor 555 donkey anti-mouse IgG (H+L) (Abcam; Material No. ab150106; 1:250) for two days at 4 °C.
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bioRxiv - Neuroscience 2021Quote: ... were subjected to western blotting using the indicated primary (mouse anti-GABAA receptor alpha 1 #ab3299, Abcam, 1:200; rabbit antibeta actin #ab8227, Abcam, 1:1000) and secondary antibodies ...
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bioRxiv - Microbiology 2022Quote: ... Secondary antibodies used for western blotting included HRP-conjugated goat anti-Rabbit and sheep anti-Mouse antibodies (Abcam, ab205718 and ab6808, respectively).
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bioRxiv - Developmental Biology 2022Quote: ... for 1 h at RT and directly labelled overnight at 4°C with primary antibodies (1:400 mouse mAB for sarcomeric alpha-actinin [CloneEA-53, Sigma-Aldrich, A7811] and 1:200 rabbit pAB for cardiac Troponin T [Abcam, ab45932]). After washing cells once with PBS ...
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bioRxiv - Immunology 2022Quote: ... Slides containing 4 sections per slide and 8 sections per sample were incubated with primary antibodies at 4°C overnight and then washed three times with PBS before incubating them with goat anti-mouse IgG (H&L) secondary antibody (Alexa Fluor® 488 in 1% BSA PBST, 1:300; Abcam #ab150113) for 1 h at room temperature in the dark ...
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bioRxiv - Cell Biology 2022Quote: ... The membrane was washed in TBST and incubated for 45 min in TBST containing 4% dried milk and HRP mouse anti-beta Actin AC-15 (1:25000, abcam Ref. ab49900). The membrane was washed in TBST and incubated 1 min in ECL Western Blotting Substrate (Thermo Fisher Scientific Ref ...
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bioRxiv - Genetics 2022Quote: ... The membrane was blocked with 5% milk in TBST (B1009, Applygen, Beijing, China) and incubated with a primary mouse monoclonal 1D4(1:1000 dilution, ab5417, Abcam, Cambridge, MA)) ...
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bioRxiv - Neuroscience 2022Quote: ... Cells were incubated overnight at 4 °C with primary antibodies — targeting α-syn (Mouse anti α-syn, AA 124-134, ab1903, Abcam; Mouse anti α-syn 4B12 ...
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bioRxiv - Physiology 2023Quote: ... and the anti-alpha 1 Na+/K+-ATPase mouse monoclonal antibody (Na+/K+-ATPase α1, #ab7671, Abcam, Cambridge, United Kingdom, 1:1000) were used to verify equal protein loading ...
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bioRxiv - Physiology 2022Quote: ... Total protein levels were measured by BCA assay (Pierce) and a 1:00 dilution was used for quantifying α-synuclein using the mouse α-synuclein ELISA kit (Abcam) following the manufacturer’s instructions.
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bioRxiv - Genetics 2022Quote: ... 1:1000; Anti-GAPDH mouse, Abcam ab8245, 1:10,000; Anti-nitrotyrosine, Merck 05-233, 1:100; Anti-GAPDH rabbit, Abcam ab9485, 1:1000), 3x 5-min washes with PBS-T ...
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bioRxiv - Developmental Biology 2022Quote: ... 1:250 mouse anti-beta tubulin (E7; Developmental Studies Hybridoma Bank, Iowa City, IA) 1:250 rabbit anti-histone H3 (ab1791; Abcam, Cambridge, MA), 1:1000 Anti-phospho-Histone-H3(ser10 ...
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bioRxiv - Cancer Biology 2024Quote: ... Samples were then incubated overnight with the following primary antibodies diluted in PBS with 0.1% Tween and 1% BSA: rabbit anti-mouse RPA2 (Abcam; ab76420; 1:50), rat anti-mouse RPA2 (CST ...
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bioRxiv - Biochemistry 2024Quote: ... The cell lysates were boiled in SDS-PAGE loading buffer for 10 min and then subjected to SDS-PAGE and probed with the following specific antibodies: mouse anti-TDP-43 monoclonal antibody (Abcam, ab57105,1:1000), rabbit anti-TDP-43 polyclonal antibody (Proteintech ...
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bioRxiv - Cell Biology 2024Quote: ... for 30 min at room temperature and incubated with the primary antibody against mouse UCP1 (ab234430, Abcam, UK; diluted at 1:500) at 4°C for 12 h ...
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bioRxiv - Neuroscience 2024Quote: ... sections were incubated overnight at 4 ℃ in an antibody diluent (1.1% Triton-X and 2% normal goat serum in PBS) containing primary antibodies anti-TUBB3 (mouse, 1:1000, Sigma-Aldrich, T8660; rabbit, 1:300, Abcam, ab18207), anti-SOX2 (rabbit ...
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bioRxiv - Neuroscience 2024Quote: ... Triton X-100 in PBS and incubated with donkey-anti-mouse IgG (H+L) Alexa Fluor 488 nm (1:500, Abcam, UK, ab150109), donkey-anti-rabbit IgG (H+L ...
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bioRxiv - Immunology 2024Quote: ... samples were treated with unconjugated affinity purified F(ab) fragment IgG anti-mouse (H+L) for 1 hr at RT (0.1 mg/ml, Abcam, cat no. ab6668). Mouse epithelium was stained with Alexa 633-conjugated phalloidin (Invitrogen ...
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bioRxiv - Immunology 2023Quote: ... The plates were washed three times with PBST and then incubated with anti-mouse IgG or IgA secondary antibody conjugated to horseradish peroxidase (HRP) (Abcam, Cambridge, UK) in PBST for 1 h at room temperature ...
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bioRxiv - Cell Biology 2023Quote: ... Immuno-staining was conducted using mouse anti-β-tubulin 1E1-E8-H4 antibody (dilution 1:300, cat. nr. ab131205, Abcam, Cambridge, UK), or goat anti-collagen Iα1 antibody (dilution 1:20 ...
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bioRxiv - Developmental Biology 2023Quote: ... The following primary antibodies were used: anti-SOX2 diluted 1:500 (R&D AB2018, mouse) and anti-NR2F1 diluted 1:1000 (Abcam Ab181137, rabbit). Sections were then washed 2X for 10 minutes in PBS1X and incubated for 1h at room temperature with blocking solution containing the following fluorescent secondary antibodies ...
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bioRxiv - Molecular Biology 2022Quote: ... Ectopic huLiver tissue was processed and stained using the methods described above with polyclonal rabbit anti-MSP1 (1:100) and monoclonal mouse anti-EBA-175 (1:100) followed with goat anti-rabbit IgG (1:100) (Abcam, Cambridge, UK), and goat α-mouse IgG1 (1:100 ...