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Citations for abcam :
4801 - 4850 of 5060 citations for Recombinant Mouse Gfra1 His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2024Quote: ... secretory IFN-β in supernatant and 2’3’-cGAMP in primary cells (neuron, astrocytes, and microglia) lysate after Aβ or gAβ treatment were quantified by mouse IFN-β (abcam) or mouse 2’3’-cGAMP ELISA kit (Cayman Chemical ...
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bioRxiv - Neuroscience 2024Quote: ... Secondary antibodies used were goat anti-mouse IgG conjugated to Alexa Fluor 488/568 (1:1000; ab150113/ab175473, Abcam, Cambridge, UK) and goat anti-rabbit IgG conjugated to AlexaFluor 488/568 (1:1000 ...
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bioRxiv - Neuroscience 2024Quote: ... Primary antibodies were against GluN2A subunit (rabbit anti-GluN2A 1:200, Alomone AGC-002) combined to mouse anti-TH (1:1000, Immunostar 22941, Abcam, UK), or GluN2B subunit (rabbit anti-GluN2B 1:200 ...
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bioRxiv - Bioengineering 2024Quote: ... sections were washed three times with PBS and incubated with the secondary antibody (goat anti-mouse Alexa Fluor 488, 1:400, Abcam 150113) for 2 hr at room temperature ...
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bioRxiv - Biochemistry 2024Quote: ... the wells were washed 6 times with wash buffer and then incubated with 100 µL per well of mouse biotinylated anti-DDDDK (Abcam ab173832) at 1:3000 in dilution buffer overnight at 4°C ...
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bioRxiv - Bioengineering 2024Quote: ... the samples were incubated with the goat polyclonal secondary antibody to Mouse IgG - Alexa Fluor® 555 (Abcam, ab150114, 1/500) in 1 % BSA solution for 2 hours at room temperature in the dark ...
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bioRxiv - Neuroscience 2024Quote: ... Incubation with the primary antibodies (anti-aSyn Syn-1 mouse, BD Transduction Laboratories 1:1000; anti-pS129-α-syn Rabbit Abcam Ab51253 ...
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bioRxiv - Neuroscience 2024Quote: ... followed by incubation with appropriate secondary antibodies (anti-rabbit AlexaFluor-488, Invitrogen #A11008, anti-mouse AlexaFluor-568, Invitrogen #A11004, and anti-chicken AlexaFluor-647, Abcam #ab150175) for 1 hour at RT ...
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bioRxiv - Microbiology 2024Quote: ... cells were washed 3X with 200 µL cold PBST before resuspension in 100 µL cold PBST containing a 1:200 dilution of goat α-mouse antibodies conjugated to AlexaFluor555 (Abcam). Cells were incubated for 1 h at 4 °C with end-over-end rotation before washing 3X with 200 µL cold PBST ...
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bioRxiv - Microbiology 2024Quote: ... the cell lysate was split into equal volumes and combined with 2 µg mouse mAb anti-V5 (SV5-PK1) (Abcam, ab27671), mouse mAb anti-HA (clone HA-7)(Merck ...
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bioRxiv - Cell Biology 2020Quote: ... Tissue sections were double labelled overnight at 4°C with antibodies against anillin (mouse or rabbit) and either CD34 (1:250, Abcam, Cambridge, UK), CD56 (1:200 ...
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bioRxiv - Cell Biology 2020Quote: Antibodies used in the study were: (WB: western blot, IF: immunofluorescence, FC: Flow cytometry): Mouse monoclonal antibodies raised against SNX27 antibody (clone 1C6, Abcam, Ab77799, WB), GFP (clones 7.1/13.1 ...
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bioRxiv - Cell Biology 2020Quote: ... Abberior® STAR orange 561 goat anti-mouse (Abberior GmbH) (1:500) and Alexa Fluor® 488 conjugated goat anti-guinea pig (Abcam, ab150185). The Abberior ® secondary antibodies were kindly provided by Abberior instruments.
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bioRxiv - Microbiology 2021Quote: ... FliC and SigA proteins on the gel were detected with anti-FliC and anti-SigA primary antibody (1:1000) and horseradish peroxidase-conjugated secondary antibody goat anti-mouse (Cat: ab97023, IgG, 1:3000, Abcam, Cambridge, MA) by Western blot ...
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bioRxiv - Immunology 2021Quote: ... which recognizes mouse fibrinogen was purchased from Thermo Fisher Scientific (Waltham, MA) and the rabbit polyclonal antibody directed against the mouse fibrinopeptide A (catalog number ab103648) was from Abcam (Cambridge, MA). The mouse anti-collagen 1α (catalog number sc-293182 ...
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bioRxiv - Developmental Biology 2020Quote: ... brains were incubated with conjugated fluorescent secondary Alexa Fluor 555 donkey anti-mouse IgG (H+L) (Abcam; Material No. ab150106; 1:250) for 2 days at 4 °C.
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Systemic impact of the expression of the mitochondrial alternative oxidase on Drosophila developmentbioRxiv - Biochemistry 2022Quote: ... and incubated with a 1% nonfat milk/PBST solution containing the primary antibodies anti-AOX (rabbit polyclonal, 1:20,000) (10) and anti-ATP5A (mouse monoclonal, Abcam, UK, 1:10,000) for 3 h at room temperature under constant shaking ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were then incubated for 60 min at room temperature with primary antibody dilutions prepared in 3 % BSA in PBS as follows: mouse anti-Bub1 (ab54893, Abcam, 1:500); mouse anti-Mad2 (sc-65492 ...
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bioRxiv - Neuroscience 2021Quote: ... membrane was probed with the following primary antibodies for overnight at 4°C: Rabbit anti-mouse LINE-1Orf1p (Abcam, ab216324, 1:1000), Mouse anti-GAPDH antibody (HRP conjugated ...
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bioRxiv - Neuroscience 2021Quote: ... Abcam; mouse anti-CD68, 1:400, Cell Signaling Technology; rabbit anti-Arg-1, 1:400, Abcam; rabbit anti-iNOS, 1:50, Abcam; goat anti-DCX ...
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bioRxiv - Neuroscience 2021Quote: ... Then the cells were blocked in 10% donkey serum for 2 h and incubated with primary antibodies (goat anti-Iba1, 1:400, Abcam; mouse anti-CD68, 1:400, Cell Signaling Technology; rabbit anti-Arg-1, 1:400, Abcam; rabbit anti-iNOS ...
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bioRxiv - Molecular Biology 2020Quote: ... Unbound antibody was removed by five 5-min washes and the membrane was incubated with alkaline phosphatase-conjugated goat anti-mouse secondary antibody or goat anti-human IgG secondary antibody (Abcam, Cambridge, UK). Membranes were washed again and then developed using SuperSignal ELISA Pico Chemiluminescent Substrate Kit (Thermo Fisher Scientific).
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bioRxiv - Cell Biology 2019Quote: ... or rabbit anti-TgTOM40 (1:2000, (van Dooren et al., 2016)) antibodies coupled to secondary horseradish peroxidase (HRP)(Promega for mouse and rabbit, Abcam for rat) conjugated antibodies (1:10,000 ...
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bioRxiv - Genomics 2020Quote: ... Blocking with 1% BSA/PBS for 1h was followed by incubation with the following primary antibodies for 1-2 h at the indicated dilution: mouse monoclonal anti-HMGB1 (1:1000; Abcam ab190377-1F3); rabbit polyclonal anti-HMGB2 (1:1000 ...
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bioRxiv - Immunology 2019Quote: ... The various antibodies used for Western blot were from Biotechne (anti-mouse IL-1β, AF401, and secondary HAF-109) or from Abcam (anti-tubulin) and Sigma-Aldrich (anti-vinculin) ...
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bioRxiv - Cell Biology 2019Quote: ... membranes were incubated over night at 4°C with mouse anti-phosho-PP1 antibody (Wu et al., 2009) (pospho-T320 Abcam, 1:1000) After washing ...
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bioRxiv - Cell Biology 2019Quote: ... 1:400, NCL-Lam-A/C, Novocastra, Leica Biosystems, Wetzlar, Germany or mouse monoclonal IgG1, 1:400, clone JOL2, AbCam, Cambridge, UK) to detect human nuclei ...
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bioRxiv - Molecular Biology 2021Quote: ... an anti-GFP monoclonal antibody (mFX75 Wako; 1:2,000 dilution) and an alkaline phosphatase-conjugated anti-mouse IgG (ab97020 Abcam; 1:20,000 dilution) were used as primary and secondary antibodies ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were then incubated with appropriate primary antibodies prepared in blocking buffer against glucagon (mouse antiglucagon antibody, Cat # ab10988, Abcam; 1:1000 or rabbit anti-glucagon antibody, Cat# ab92517, Abcam; 1:25), Stathmin-2 (goat anti-SCG10 antibody ...
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bioRxiv - Cell Biology 2021Quote: ... goat anti-mouse IgG Alexa Fluor® 488 (ab150117) and Goat ant-rabbit Alexa Fluor® 647 conjugated (ab150091) were from Abcam, UK ...
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bioRxiv - Neuroscience 2021Quote: ... and then incubated in secondary antibodies (donkey antichicken Alexa 488, Jackson ImmunoResearch 703-545-155; donkey anti-mouse Dylight 550, Abcam ab96876, donkey anti-rabbit Dylight 650, Abcam ab96894) diluted in a blocking solution for one hour at RT ...
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bioRxiv - Neuroscience 2021Quote: ... Horseradish peroxidase-labeled conformation-sensitive secondary anti-mouse IgG for IP or anti-rabbit VeriBlot for IP Detection antibody (both at 1:20000; Abcam, Cambridge, MA) was detected by SuperSignal West Femto Maximum Sensitivity substrate (Thermo Fisher Scientific).
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BRD2 inhibition blocks SARS-CoV-2 infection by reducing transcription of the host cell receptor ACE2bioRxiv - Cell Biology 2021Quote: ... Antibodies in fresh 5% NFDM were added (Mouse monoclonal GAPDH 1:10,000; Goat polyclonal ACE2 [R&D Tech AF933] 1:200; Rabbit monoclonal BRD2 [abcam 197865] 1:5,000) and incubated at 4 °C for at least 16 hours ...
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bioRxiv - Neuroscience 2020Quote: ... for 1 hr at room temperature and then incubated with the following primary antibodies (diluted in 5% non-fat milk in 0.1% PBS-Tween 20) for 18 hrs at 4°C: Mouse monoclonal Total OXPHOS rodent WB cocktail (Abcam ab110413, 1:1,000); mouse monoclonal Total OXPHOS blue-native WB cocktail (Abcam ab110412 ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were washed and incubated under darkness at RT with a goat anti-mouse secondary antibody (1:500, Alexa fluor 488, Abcam, Cambridge, UK) in 0.1% Tx-100 ...
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bioRxiv - Immunology 2020Quote: ... The ELISA plates were washed 4 times by Blocking Buffer and ALP-conjugated Goat Anti-Mouse IgG Fc (Abcam, ab98710, 1:1000) in 50 μL Blocking Buffer was added ...
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bioRxiv - Neuroscience 2020Quote: ... and incubated overnight at 4° C with the primary monoclonal mouse antibody MOAB-2 against beta amyloid (1:500; Abcam Cat# ab126649) (Youmans et al. ...
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bioRxiv - Neuroscience 2020Quote: ... every fourth section throughout the hippocampus/dentate gyrus was collected and stained with panel 3 = anti-mouse BrdU (ab8152, Abcam, 1:500), anti-mouse doublecortin (DCX ...
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Targeted attenuation of elevated histone marks at SNCA alleviates α-synuclein in Parkinson’s diseasebioRxiv - Neuroscience 2020Quote: ... the following primary antibodies were used: (Normal mouse IgG, Millipore, 12-371; H3K4me3, Abcam, ab8580; H3K27me3, Active motif, 39155; H3K27ac, Abcam, ab4729). Anti-Cas9 antibody (Takara ...
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bioRxiv - Pathology 2021Quote: ... KIM-1 was also measured in urine as a marker for PT injury using a mouse ELISA kit (Abcam, Cambridge, MA; ab119596). As a marker of distal nephron injury ...
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Role of autophagy in sepsis-induced skeletal muscle dysfunction, whole-body metabolism, and survivalbioRxiv - Cell Biology 2021Quote: ... Membranes were blocked in PBS + 1% Tween® 20 + 5% BSA for 1 h at room temperature then incubated overnight with specific primary antibodies at 4°C.Membranes were washed in PBST (3×5 min) then incubated with HRP-conjugated goat anti-rabbit (ab6721) or rabbit anti-mouse (ab6728) secondary antibodies (Abcam, Cambridge, MA) for 1 h at room temperature ...
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bioRxiv - Cell Biology 2020Quote: ... These were then washed three times with PBS and labelled with antibodies directed against RPE cell markers including mouse monoclonal anti-RPE65 (Abcam, clone: 401.8B11.3D9) and anti-bestrophin-1 (Novus Biologicals ...
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bioRxiv - Cell Biology 2020Quote: ... and incubated in suitable 10 nm (anti-mouse) or 20 nm (anti-rabbit) gold particle-conjugated secondary antibodies (Abcam ab39619 and ab27237) for 60 min (diluted 1:10 with 0.1% BSA in PBS1X) ...
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bioRxiv - Biophysics 2020Quote: ... Secondary antibodies were applied for 1 hour (goat anti-mouse Alexa Fluor 488 pre-adsorbed abcam, 1:750 dilution and goat anti-rabbit Alex Fluor 594 pre-adsorbed abcam, 1:750). Nuclei were stained with DAPI (Roche)
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bioRxiv - Cell Biology 2021Quote: ... CST), anti-Lamin A/C mouse monoclonal antibody (Abcam, Cat # ab8984, Anti-non-muscle Myosin IIA rabbit polyclonal antibody (Abcam, Cat #ab75590), Anti-non-muscle Myosin IIB mouse monoclonal antibody (Abcam ...
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bioRxiv - Neuroscience 2022Quote: ... Primary antibodies (Goat anti-GFP biotin conjugated, Vector Labs; sheep polyclonal anti-human-sema3f antibody, R&D systems; mouse anti-GADPH, Abcam) were diluted in the blocking buffer ...
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bioRxiv - Neuroscience 2022Quote: ... followed by an overnight incubation at 4°C with the primary antibodies rabbit anti-ATXN3 (anti-MJD 1:20000)42 or mouse anti-GAPDH (1:1000, ab9484 Abcam, Cambridge, UK), and subsequent incubation with the corresponding peroxidase-conjugated anti-rabbit or anti-mouse secondary antibodies (1:2000 ...
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bioRxiv - Immunology 2022Quote: ... The plates were washed three times with PBST and incubated with horseradish peroxidase-conjugated goat anti-mouse IgG (Abcam, ab6789, 1:10,000), goat anti-mouse IgG1 (Abcam ...
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bioRxiv - Biophysics 2022Quote: ... cells were fixed in ice-cold methanol at −20 before washing three times in PBS containing 10% horse serum (HS) for 5min each and being incubated with a mouse monoclonal primary antibody against α-tubulin (DMA1, 1:1000 dilution, Abcam, UK) for 1 hour at RT ...
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bioRxiv - Cell Biology 2022Quote: ... incubated with FITC-conjugated α-CD83 and α-CD86 abs as well as with a primary mouse α-RTN1A ab (mon162, abcam) ON at 4°C ...