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Citations for abcam :
4651 - 4700 of 5060 citations for Recombinant Mouse Gfra1 His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2020Quote: ... Commercially available polyclonal (pAb) or monoclonal (mAb) antibodies were used for beta Actin (HRP conjugated; Mouse mAb Abcam, [AC-15] ab49900), Tubulin (Mouse mAb ...
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bioRxiv - Cell Biology 2019Quote: ... was purchased from BD Biosciences. Mouse monoclonal anti-α-adaptin (Cat. Nr. ab2807) and rabbit monoclonal anti-EGFR (Cat. Nr. ab2430) were purchased from Abcam. Gefitinib (Cat ...
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bioRxiv - Cell Biology 2020Quote: ... the cells were incubated at 4°C with one of the following mouse monoclonal primary antibodies: anti-heavy chain cardiac myosin (Abcam, #ab50967), anti-α-actinin (Sigma-Aldrich ...
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bioRxiv - Immunology 2021Quote: ... Intracellular HO-1 staining for human and mouse was performed using the Invitrogen FIX & PERM Kit (GAS003) using HO-1 PE at 1:100 dilution (Abcam, ab83214) and PE IgG2b isotype (Abcam ...
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bioRxiv - Cell Biology 2021Quote: Primary antibodies were as follows: monoclonal mouse anti-AURKA (Clone 5C3; (Cremet et al., 2003) at 1:20 and anti-TOMM22 (Abcam, ab10436) at 1:5000 ...
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bioRxiv - Developmental Biology 2020Quote: ... either mouse anti Hira (LSBio LS-C137477) and rabbit anti Ubn1(LSBio LS-B11373) or mouse anti Hira and rabbit anti Cabin1 (Abcam ab3349). As a negative control the antibodies raised in rabbit were incubated with mouse anti hCG (Abcam ab9582 ...
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bioRxiv - Neuroscience 2021Quote: ... and incubated in secondary antibodies (1:500, Alexa 488 donkey anti-rabbit, Invitrogen, A21206, and 1:500, Alexa 647 donkey anti-mouse, Abcam, ab181292) at room temperature overnight ...
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Diversified expression of gamma-protocadherins regulates synaptic specificity in the mouse neocortexbioRxiv - Neuroscience 2021Quote: An ICR mouse at p11 were perfused with dissociation-artificial cerebrospinal fluid (aCSF) (choline-aCSF with 50 μM DL-APV (Abcam/ab120271), 10 μM DNQX (Sigma/D0540) ...
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bioRxiv - Bioengineering 2022Quote: ... A solution of 0.05% of Tween-20 was used to wash the samples and a solution of goat anti-Mouse secondary antibody IgG was added (1:1000, Abcam # ab6785) and incubated for 2 h at room temperature ...
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bioRxiv - Bioengineering 2019Quote: ... and processing the samples for immunocytochemistry using mouse monoclonal Tf antibody (Serotech 9100-1057) and rabbit polyclonal TfR antibody (Abcam ab84036) diluted 1:250 ...
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bioRxiv - Developmental Biology 2020Quote: ... alpha-tubulin and histone H3 were detected using monoclonal antibody (mouse anti-alpha-Tubulin, Sigma T9026 or rat anti-Tubulin, Abcam ab6160) and polyclonal antibody (rabbit anti-H3 ...
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bioRxiv - Cancer Biology 2019Quote: ... the first wash with wash buffer A after ON hybridization contained 1:800 diluted anti-mouse secondary antibody labeled with Alexa Fluor 488 (Abcam, ab150113).
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bioRxiv - Biochemistry 2020Quote: ... Membranes were treated with antibodies diluted 1:1000 in 0.5% BSA and washed 3 times for 5 min before applying secondary antibiotic (anti-mouse or anti-goat from Santa Cruz Biotechnology and Anti-Rabbit from Abcam at a concentration of 1:1000 ...
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bioRxiv - Molecular Biology 2019Quote: ... They were then incubated overnight at 4°C with mouse monoclonal antibody against tyrosine hydroxylase (TH) (1:750, Abcam, Cambridge, UK) or goat polyclonal primary antibody against cathepsin X (1:200 ...
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bioRxiv - Immunology 2021Quote: ... All steps with tissue sectioning and IHC are the same as with mouse tissue through blocking in 10% normal donkey serum (Abcam, ab7475) for 20 minutes at RT ...
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bioRxiv - Biochemistry 2021Quote: ... for 10 min and then incubated with the following secondary antibody for 2 hrs: goat anti-mouse IRDYe® 680 conjugated antibody (1:10,000; abcam; ab216776). The membrane was washed again with PBST three times for 10 min and imaged on the Odyssey Li-Cor.
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bioRxiv - Neuroscience 2020Quote: ... Primary antibodies were as follows: mCherry (used for mRuby, mCherry and TdTomato, made in mouse, monoclonal, ABCAM AB167477, concentration 1:1000), ChAT (choline acetyltransferase ...
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bioRxiv - Biochemistry 2020Quote: ... The membrane was immunoblotted with primary antibodies: mouse anti-strep (1:2500, Fisher NBP243719) and rabbit anti-thiophosphate ester (1:2000, Abcam ab133473), washed ...
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bioRxiv - Bioengineering 2020Quote: ... Sections were blocked with PBS-BSA 5% w/v for 30 min at RT and incubated with the primary antibodies (collagen type II, mouse monoclonal antibody, DSHB, II-II6B3, 1:100; collagen type I, rabbit monoclonal antibody, Abcam, ab138492) overnight at 4°C ...
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bioRxiv - Neuroscience 2021Quote: ... the membrane was washed in 1X tris buffered saline containing 0.1% Tween20 (TBS-T) and incubated over-night with mouse anti-V5 (1:250; Thermo, R960-25) or anti-Gapdh (1:3000; Abcam, ab8245) diluted in Start Block ...
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bioRxiv - Neuroscience 2020Quote: ... Tissue was then incubated for 2 hours at room temperature in a PBTGS secondary antibody solution including goat anti-mouse Alexa568 (1:200; Abcam [ab175473]), goat anti-rabbit Cy2 (1:200 ...
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bioRxiv - Microbiology 2021Quote: ... then incubated overnight at 4°C with the primary antibody mouse anti-SA Hla [8B7] -N-terminal (Abcam, Cambridge, United Kingdom) diluted 1:2500 in the blocking buffer ...
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bioRxiv - Immunology 2021Quote: ... staining with primary and secondary antibodies were realized according to the manufactures of the following IHC kits: Mouse and rabbit specific HRP/DAB IHC detection Kit micro-polymer (Abcam, Cat.ab236466) and ImmPRESS[R] Duet double staining kit anti-rabbit AP/anti-mouse HRP (Vector laboratories ...
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bioRxiv - Cancer Biology 2021Quote: ... a pre-diluted mouse monoclonal anti-p53 (Clone Bp53-11, Ventana Medical Systems, Inc.) and a rabbit polyclonal anti-CDC20 (Abcam #ab72202) at 1:300 were manually applied for 32 min at 37°C ...
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bioRxiv - Neuroscience 2020Quote: ... followed by a 2 hr incubation with a mixture of the secondary antibody (goat anti-mouse Alexa Fluor® 488–conjugated IgG, ab150113 Abcam) and Alexa Fluor 568 conjugated Phalloidin (A12380 ...
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bioRxiv - Bioengineering 2020Quote: ... Richard-Allan Scientific) and α-smooth muscle actin (α-SMA) (primary Rabbit anti-mouse alpha-SMA antibody; Cat. No. ab5694; Abcam; 1:50 dilution ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were washed and incubated in darkness with goat anti-mouse secondary antibodies (1:500, Alexa fluor 488, Abcam, Cambridge, UK) in 0.1% Tx-100 ...
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bioRxiv - Molecular Biology 2021Quote: ... cells were permeabilized using 0.1% Triton-X and incubated for 2 hours at room temperature with mouse monoclonal antibody against sarcomeric α-actinin (1:100; Abcam), followed by an incubation of 1 hour with the secondary antibody conjugated to Alexa Fluor-488 (Life Technologies) ...
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bioRxiv - Immunology 2021Quote: ... Fixed cells were blocked with 5% BSA in PBS for 1 hr at room temperature and incubated with rabbit anti-mouse Ki67 primary antibody (Abcam ab15580) at 1:200 dilution overnight at 4°C ...
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bioRxiv - Cell Biology 2021Quote: ... and incubated at RT for 2 h with mouse anti-CTLA-4 monoclonal antibody (diluted: 1:3 000; Abcam, Cambridge, UK) as the primary antibody ...
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bioRxiv - Biophysics 2020Quote: ... the samples were incubated with the appropriate secondary antibody (polyclonal goat anti-mouse IgG H&L, 1:1000, Abcam, Cambridge, UK) diluted in blocking solution for 45 min at room temperature ...
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bioRxiv - Cell Biology 2021Quote: ... cells were incubated 45 min at room temperature with the respective secondary antibodies: anti-mouse Alexa-Fluor® 594 (abcam, ab150108), anti-chicken Alexa-Fluor® 633 (Sigma ...
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bioRxiv - Cell Biology 2021Quote: ... slides were thoroughly washed using PBS 1X/Triton- X 0.1% and incubated overnight at 4°C with the respective antibodies anti-mouse Alexa-Fluor® 594 (abcam, ab150108), anti-sheep Alexa-Fluor® 488 (Jackson Immunoresearch ...
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bioRxiv - Cell Biology 2022Quote: ... The coverslips were incubated with the primary antibodies (rabbit anti-TPR, 1:100, NB100-2867; and mouse anti-NUPs, 1:100, Abcam 24609) in blocking solution overnight at 4°C ...
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bioRxiv - Biophysics 2022Quote: ... Membranes were washed 3 times for 10 minutes at room temperature with TBST and incubated with either anti-Mouse IgG-HRP (1:20 000, Abcam, ab6728) or anti-Rabbit IgG-HRP (1:20 000 ...
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bioRxiv - Bioengineering 2022Quote: ... samples were washed x3 times with PBS and incubated for 2 h at room temperature with corresponding secondary antibody (α-mouse. 488, 1:500, ab150077, abcam; α-rabbit ...
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bioRxiv - Bioengineering 2022Quote: ... samples were washed x3 times with PBS and incubated for 2 h at room temperature with corresponding secondary antibody (α-mouse. 488, 1:500, ab150077, abcam; α-rabbit. 488,1:500, ab150077, abcam; α-goat ...
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bioRxiv - Developmental Biology 2022Quote: ... and rabbit polyclonal anti-RAD51 (1:1000, (75)), anti-SYCP1 (1:2500, (76)) and mouse monoclonal anti-DMC1 (1:1000, Abcam, ab11054), anti-SYCP3(1:200 ...
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bioRxiv - Cell Biology 2022Quote: ... Primary antibodies were rabbit anti-SEPT7 (1:400, IBL 18991) and mouse anti-non-muscle Myosin IIA (1:200, abcam ab55456). Secondary antibodies were goat AlexaFluor488-conjugated anti-mouse IgGs (1:400 ...
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bioRxiv - Microbiology 2022Quote: ... Spain) binding to the 229E nucleocapsid protein and a goat anti-mouse secondary antibody conjugated with fluorescein isothiocyanate (Abcam, United Kingdom). TCID50 titers were calculated according to the method of Reed and Muench (44).
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bioRxiv - Neuroscience 2022Quote: ... and validated primary antibodies: mouse anti-CaVβ4 (Neuromab #75-054, 1:1000) and rabbit anti-β-tubulin (Abcam #ab6046, 1:600,000). The membrane was then incubated in LiCor Blocking Buffer diluted 1:1 with TBS (0.1% Tween 20 and 0.02% SDS ...
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bioRxiv - Microbiology 2022Quote: ... Slides were washed once again and HRP-conjugated secondary antibody was added at a 1:5000 concentration (Goat anti-mouse: ThermoFisher, Cat #A21426; Goat anti-rabbit: Abcam, Ab6721). Slides were washed twice prior to application of DAB developing reagent (Vector Laboratories ...
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bioRxiv - Bioengineering 2022Quote: ... ECM components were detected using mouse mAbs specific for collagen type I (0.5 μg/ml clone ab90395, Abcam®, Cambridge, UK), collagen type IV (2 μg /ml clone M0785 ...
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bioRxiv - Cell Biology 2022Quote: Epidermal punch biopsies (6 mm diameter) in triplicates were floated on medium containing either a mouse α-RTN1A ab (mon162, abcam) or the respective isotype control ab (IgG1 ...
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bioRxiv - Cancer Biology 2022Quote: ... Coverslips were then washed with 1xPBS 3x for 5 minutes before incubation in 50 µl spots of secondary antibodies (goat anti-mouse AF647: Abcam ab150115, goat anti-rabbit AF405: abcam 175652) diluted in incubation buffer for 1hr at RT ...
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bioRxiv - Microbiology 2021Quote: ... horseradish peroxidase (HRP)-conjugated goat anti-mouse antibodies against the light chain (Fab) and heavy chain (Fc) (Abcam,plc, Cambridge, MA), and HRP-conjugated goat anti-rabbit antibody (Abcam ...
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bioRxiv - Neuroscience 2021Quote: ... and then incubated in secondary antibodies (donkey antichicken Alexa 488, Jackson ImmunoResearch 703-545-155; donkey anti-mouse Dylight 550, Abcam ab96876 ...
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bioRxiv - Immunology 2020Quote: Transferrin and cytokines in the samples (cell supernatant or plasma) were measured using a mouse transferrin ELISA kit (ab157724, Abcam, USA), mouse TNF-α ELISA kit (DKW12-2720-096 ...
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bioRxiv - Neuroscience 2021Quote: ... 0.1% SDS) and incubated overnight at 4°C with 3 μL specific mouse monoclonal antibody recognizing Bcl11a (Abcam Cat# ab19487, RRID:AB_444947) or unspecific IgG1 antibody (Cell Signaling Technology Cat# 5415 ...
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bioRxiv - Microbiology 2021Quote: ... the samples were incubated with goat anti-mouse IgG Alexa Fluor 488 secondary antibody (1:1000 dilution; Abcam, Cambridge, MA, USA) for 1 h at 25℃ and the slides were mounted in antifade Mounting Medium with DAPI (Vectashield ...