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Citations for abcam :
4651 - 4700 of 4741 citations for PLGF Mouse HEK 293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2019Quote: ... For the localization of total fraction of RNAPII we used mouse 8WG16 antibody (anti-RNA polymerase II CTD repeat YSPTSPS, Abcam, diluted 1:200), according to protocols provided by Kołowerzo-Lubnau et al64 ...
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bioRxiv - Biochemistry 2020Quote: ... blots were incubated overnight at 4°C with a mouse antibody against the α subunit of Na+/K+-ATPase (Abcam-ab7671, 1:2,000) and with a mouse antibody against the His-tag (Tetra·His Antibody ...
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bioRxiv - Cell Biology 2020Quote: ... with fibronectin-depleted (depleted via column purification using gelatin sepharose) FBS at 10% on glass coverslips coated with 10ug/mL murine fibronectin (native mouse fibronectin, Abcam, Cambridge MA, USA) or laminin (laminin mouse protein ...
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bioRxiv - Cell Biology 2019Quote: ... Rabbit polyclonal HPA antibodies targeting the proteins of interest were dissolved to 2-4 μg/ml in blocking buffer (PBS + 4% FBS) containing 1 μg/ml mouse anti-tubulin (Abcam, ab7291, Cambridge, UK). After washing with PBS ...
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bioRxiv - Physiology 2019Quote: ... for 1 h at room temperature and next incubated with primary antibodies (p62, CoxIV: Cell Signaling,3E11, Tomm20:Abcam, UK, ab56783 (anti-mouse) or ab186734 (anti-rabbit) ...
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bioRxiv - Cell Biology 2021Quote: ... Membranes were then probed with Alexa Fluor anti-rabbit A680 secondary antibody and Alexa Fluor anti-mouse A790 secondary antibody (Abcam ab175773 and ab175783). Membranes were visualized using an Odyssey Infrared Imaging System (LI-COR) ...
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bioRxiv - Biochemistry 2021Quote: ... the chambered coverglass plates were rinsed three times with PBS and cells were labeled with fluorescently conjugated secondary antibody (anti-Rabbit Alexa Fluor 488, lnvitrogen #A-11008, 1:500; anti-Mouse Alexa Fluor 647, Abcam# ab150119, 1:500) in blocking buffer for one hour at room temperature ...
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bioRxiv - Biochemistry 2021Quote: ... then incubated in 5% milk in TBS-T containing 1:10000 horseradish peroxidase (HRP) conjugated goat anti-mouse secondary antibody (Abcam ab6789-1 MG) for 1 hour at room temperature ...
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bioRxiv - Biophysics 2021Quote: ... Western blots were imaged on an Odyssey imaging system (Licor) with anti-PAR primary and goat anti-mouse IR680 (Millipore Sigma AM80 and Abcam ab216776, respectively). A dT50 oligo was radioactively labeled with 32P by T4 PNK (NEB M0201) ...
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bioRxiv - Cancer Biology 2021Quote: ... DNA fibers were incubated with mouse-FITC-anti-bromodeoxyuridine (detects IdU, B44, Becton Dickinson, 1:50) and rat-anti-bromodeoxyuridine (detects CldU, abcam ab6326, 1:100), at 37°C for 1 hour and then with anti-mouse IgG AlexaFluor 488 (Invitrogen A11029 ...
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bioRxiv - Microbiology 2020Quote: ... the fixed cells were labeled with primary antibodies including goat anti-major outer-membrane protein (MOMP; Meridian, Memphis, TN) and mouse or rabbit anti-six histidine tag (Genscript, Nanjing, China and Abcam, Cambridge, UK, respectively). For experiments using CRISPR interference ...
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bioRxiv - Neuroscience 2020Quote: ... sections were incubated with matching secondary antibodies (anti-rabbit and anti-guinea pig were the same ones as previously used as well as an Alexa Fluor goat anti-mouse 555 [Abcam, product code: ab150114]) and a DAPI stain was added ...
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bioRxiv - Cell Biology 2022Quote: ... The fluorescent secondary antibodies (goat anti mouse IgM Alexa Fluor® 647, abcam, ab150123, or donkey anti rabbit IgG Alexa Fluor® 568, abcam, ab175470) and DAPI (SouthernBiotech ...
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bioRxiv - Plant Biology 2022Quote: ... Shanghai, CN), Goat Anti-Mouse IgG HRP (Abmart, Shanghai, CN) and Anti-Myc antibody (HRP Anti-Myc tag antibody, Abcam, Cambridge, MA, USA).
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bioRxiv - Physiology 2022Quote: All protein homogenates from human and mouse lungs or HPAECs were subjected to Western blot analysis as reported.23 Primary antibodies were purchased from Abcam (Waltham, MA, USA) and included USP11 anti-rabbit polyclonal antibody (1:250 dilution ...
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bioRxiv - Microbiology 2022Quote: ... LTA was detected with an anti-LTA primary antibody (mAb 55; HycultBiotech; 1:5000 dilution) and an HRP-goat anti-mouse IgG secondary antibody (Abcam; 1:10000 dilution). LTA was visualised using Amersham ECL Prime western blotting detection reagent (GE Healthcare ...
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bioRxiv - Molecular Biology 2022Quote: ... SUMO (two antibodies used; mouse anti-SUMO-1, clone 21C7, Zymed, #33-2400, 1:200; rabbit anti-SUMO-1, Abcam, ab32058, 1:200), rabbit anti-DAXX (polyclonal D7810 ...
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bioRxiv - Cancer Biology 2024Quote: Immunofluorescence (IF) was performed on whole FFPE 5-µm-thick tissue sections using anti-TP63 mouse monoclonal antibody (1:100; Abcam, catalog no. ab735), anti-CK8 rabbit polyclonal antibody (1:100 ...
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bioRxiv - Pathology 2024Quote: ... Primary antibodies and labels used for wholemount staining in humans included mouse monoclonal anti-CD31 (1:50) and rabbit polyclonal anti-osteopontin (SPP1, Abcam, ab8448, 1:100). Rabbit polyclonal anti-osteopontin SPP1 ...
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bioRxiv - Cell Biology 2024Quote: ... anti-Lgl (SCBT, rb 1:250), anti-aPKC (SCBT, rb 1:100), anti-Nrv (DSHB, mouse 1:100) and anti-GFP (AbCam, chicken, 1:1000). Midguts were washed 3Xs ...
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bioRxiv - Molecular Biology 2024Quote: ... Coverslips were incubated with the following primary and secondary antibodies in PBS/10 % FBS for 1 hour to overnight: mouse anti-MRE11 (abcam, #ab214, 1:500), rabbit anti-TFAM (antibodies-online ...
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bioRxiv - Molecular Biology 2024Quote: ... slides were incubated with primary antibody mix of mouse anti-BrdU which recognizes IdU (Becton Dickinson #347580, 1:250) and rat anti-BrdU which recognizes CldU (Abcam #6326, 1:250) diluted in blocking buffer for 2.5h at RT in a dark humid chamber ...
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bioRxiv - Microbiology 2023Quote: ... Midguts were washed five times via direct tube transfer in PBS+T before staining with the secondary antibody (Goat anti-mouse Alexa Fluor 594, Abcam, Cambridge, United Kingdom) at a 1:1000 dilution in blocking solution for 1 hour at room temperature ...
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bioRxiv - Microbiology 2024Quote: ... The equivalent of 0.3 OD units was analyzed by SDS-PAGE and western blot using the following antibodies and dilutions: mouse anti-AD (Abcam, cat nr ab135398) 1:200 in 3% BSA in PBS-0.5% Tween (PBS-T) ...
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bioRxiv - Genomics 2023Quote: ... cells were immunostained in blocking buffer containing mouse anti-beta III Tubulin (Tub) conjugated Alexa Fluor 488 antibody (Abcam ab195879, dilution 1:1000) and DAPI (Sigma D9542 ...
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bioRxiv - Pathology 2023Quote: ... Sections were incubated at room temperature for 1 hour with mouse monoclonal primary antibodies against rat substance P (1:300 dilution, ab14184, Abcam, Cambridge, MA, USA) or normal mouse serum (ab7486 ...
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bioRxiv - Molecular Biology 2023Quote: ... slides were incubated with primary antibody mix of mouse anti-BrdU which recognizes IdU (Becton Dickinson #347580, 1:250) and rat anti-BrdU which recognizes CldU (Abcam #6326, 1:250) diluted in blocking buffer for 2.5h at RT in a dark humid chamber ...
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bioRxiv - Cell Biology 2023Quote: ... or anti-Sc35 (MilliporeSigma, S4045, 1:1000) and incubated with the secondary antibodies using Alexa 488-labeled goat anti-mouse IgG (Abcam, Ab150113, 1:1000), Alexa 594-labeled goat anti-rabbit IgG (ThermoFisher ...
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bioRxiv - Immunology 2023Quote: ... in PBS-Tween (1:250, overnight, 4°C) and FITC anti-mouse alpha muscle actin (Abcam, ab8211-500, 1:100, overnight, 4°C). Tissues were washed with PBS-Tween before incubation with secondary antibody PE-Goat anti-Rabbit IgG (Abcam ...
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bioRxiv - Neuroscience 2023Quote: ... sections were mounted on slides and stained using a monoclonal mouse anti-muscarinic acetylcholine receptor M4/CHRM4 primary antibody (1:500, abcam, ab77956, RRID: AB_1566454), with blocking reagents and secondary anti-mouse biotinylated antibody from the M.O.M ...
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bioRxiv - Molecular Biology 2023Quote: ... Fixed cells were blocked in 1% BSA in PBS for 30 minutes at room temperature before overnight incubation at 4°C in primary antibodies: α-mouse CENP-A (1:1000 Abcam ab13939 (discontinued) or 1:1500 Enzo ADI-KAM-CC006-E ...
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bioRxiv - Bioengineering 2022Quote: ... Each well was blocked with 5% BSA-tween solution and incubated with a primary mouse anti-human α-SMA antibody (ab7817, Abcam, Cambridge, UK) solution at a 1:100 dilution overnight at 4°C ...
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bioRxiv - Cell Biology 2022Quote: ... samples were incubated with a secondary antibody diluted in 1%BSA PBS-0.05%Tween 20 (goat anti-mouse IgG H&L – Alexa fluor 488 preabsorbed, Abcam, cat# ab150081; 1:500) for 1 hour at room temperature ...
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bioRxiv - Cell Biology 2023Quote: ... After washing the membranes in TBST (3 × 5 min), they were probed (1.5 h, RT) with the HRP-conjugated secondary antibodies: goat anti-mouse IgG (Abcam Cat# ab205719, 1:10000) or goat anti-rabbit IgG (Abcam Cat# ab205718 ...
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bioRxiv - Genetics 2023Quote: ... The following primary antibodies were used at the indicated dilutions: mouse α-H3K4me3 (1:500, MAB Institute Inc Wako 305-348191) and rabbit α-H3K9ac (1:1000, Abcam ab10812). The secondary antibodies from Jackson ImmunoResearch Laboratories (West Grove ...
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bioRxiv - Biochemistry 2023Quote: ... The cell lysates were boiled in SDS-PAGE loading buffer for 10 min and then subjected to SDS-PAGE and probed with the following specific antibodies: mouse anti-PrP monoclonal antibody 8H4 (Abcam ab61409, 1:5,000), mouse anti-MyHC antibody MF-20 (Developmental Studies Hybridoma Bank MAB4470-SP ...
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bioRxiv - Systems Biology 2023Quote: ... the cells underwent an additional 30-minute blocking step using 1× blocking buffer and were then incubated for 1 hour with secondary antibodies – Goat Anti-Mouse IgG H&L (Alexa Fluor® 594, Abcam, Cambridge, UK), and Phalloidin-iFluor 488 Reagent (Abcam ...
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bioRxiv - Biochemistry 2023Quote: ... for 10 min and blocked in 0.5% BSA in 0.5% Tx-PBS for 1 h at room temperature before incubation with mouse monoclonal anti-ψ-tubulin antibody (Abcam, ab11316, 1:1000) in 0.5% BSA in 0.5% Tx-PBS overnight at 4 ºC ...
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bioRxiv - Cell Biology 2023Quote: ... Sections were washed with PBS and incubated in the blocking solution containing goat anti-mouse IgM Alexa Fluor 488 (Abcam, ab150121, 1:500) antibody and goat anti-mouse IgG H&L Alexa Fluor 594 (Abcam ...
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bioRxiv - Neuroscience 2023Quote: ... using V-PLEX Pro-inflammatory Panel 1 (Meso Scale Discovery, catalogue no. K15048D1) and mouse apolipoprotein E ELISA kit (abcam, catalogue no. ab215086) according to the manufacturers’ instructions ...
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bioRxiv - Neuroscience 2023Quote: ... DRG or spinal sections were incubated with secondary antibodies for 2 h: goat anti-mouse IgG H&L (Alexa Fluor® 488) (1:500, Abcam, Cat#: ab150113), goat anti-guinea pig IgG H&L (Alexa Fluor® 488 ...
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bioRxiv - Developmental Biology 2023Quote: ... was used as a positive control to confirm a successful experiment and a secondary antibody anti-Mouse IgG (Abcam, ab46540, 1:50 dilution) was used as negative control to remove background in the following sequencing analysis.
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Linear ubiquitination at damaged lysosomes induces local NF-κB activation and controls cell survivalbioRxiv - Cell Biology 2023Quote: ... Secondary antibody incubation was done for 1 h at RT with the following horseradish peroxidase (HRP)-coupled antibodies: HRP-conjugated goat anti-mouse IgG (Abcam Cat# ab6789, RRID:AB_955439), HRP-conjugated goat anti-rabbit IgG (Abcam Cat# ab6721 ...
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bioRxiv - Developmental Biology 2024Quote: Serum vitamin D (25(OH)D) levels of mouse serum samples were assessed by commercially available ELISA kits (Eagle Biosciences, Inc. Amherst NH, or Abcam, Cambridge, MA) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2024Quote: ... Whole cell lysates of SH-SY5Y cells (10 µg) were subjected to Western blotting using the following antibodies: mouse anti-SLP-2 antibody (Abcam ab89025, 1:1000), rabbit anti-HSP60 (Cell Signaling #4870 ...
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bioRxiv - Cell Biology 2024Quote: ... Goat anti-rabbit IgG (Alexa Fluor 488) antibody (ab150081) and goat anti-mouse IgG (Alexa Fluor 594) antibody (ab150120) were from Abcam (Cambridge, MA, USA). SAG (Smoothened Agonist ...
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bioRxiv - Developmental Biology 2021Quote: ... secondary antibodies conjugated with fluorescence dyes (1:5000; anti-mouse IRDye® 800CW and anti-rabbit IRDye® 680RD; Abcam, ab216774 and ab216779, respectively) were treated at room temperature for 3 h ...
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bioRxiv - Bioengineering 2019Quote: ... cells were resuspended at a concentration of 2×106 cells/ml in serum-free media containing 30 μg/ml of an IgG mouse isotype control antibody (abcam, Cambridge, MA, Catalog #ab91353) or a function blocking anti-β1 integrin antibody (abcam ...
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bioRxiv - Neuroscience 2021Quote: Fixed CNS tissue was cryosectioned (20 μm) and stained overnight with primary antibodies against: ERα (EMD Millipore, #C1355 polyclonal rabbit or Abcam, #93021 monoclonal mouse), phospho-Serine 244/247 RPS6 (Invitrogen ...
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bioRxiv - Neuroscience 2021Quote: ... Brain sections were incubated in primary antibody (mouse anti-TH antibody, 1:2000, cat#: MAB318, Sigma-Aldrich, St. Louis, MO, USA; or mouse anti-NET antibody, 1:1000, cat# 211463, Abcam, Cambridge, MA, USA) for 48 h at 4 °C or overnight at room temperature (RT) ...