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Citations for abcam :
4551 - 4600 of 4741 citations for PLGF Mouse HEK 293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Pathology 2020Quote: Formalin-fixed paraffin-embedded tissue sections were subjected to antigen retrieval with EDTA buffer in high pressure heated water bath and staining was performed using either mouse monoclonal ERRα (1:2500, Abcam, Cambridge, MA) or ERRγ (1:400 ...
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bioRxiv - Neuroscience 2021Quote: ... membranes were incubated for 1h with their respective secondary fluorescent antibodies: anti-mouse (1:2,500, IRDye 800, Abcam, Cat# ab216772, RRID: AB_2857338) and anti-rabbit (1:2,500 ...
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bioRxiv - Pathology 2021Quote: ... The RBD-specific IgA titer of BAL samples was next evaluated by ELISA as described above with Goat anti-mouse IgA alpha chain-HRP (Abcam, 1:3000).
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bioRxiv - Genetics 2021Quote: ... embryos were washed 3 times 10 minutes in 1x PBS with 0.1% Triton-X100 and 0.1% BSA and incubated overnight at 4°C with the secondary antibody goat-anti-mouse A568 1:500 (Abcam, antibody ID# ab175473). On the 4th day ...
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bioRxiv - Microbiology 2020Quote: ... 293T cells were fixed and processed for immunostaining against dsRNA (Scicons, 10010500) and visualized with goat anti-mouse IgG H&L (Cy5 ®) (Abcam, ab6563). Images were taken using Nikon Eclipse Ti-E Spinning Disk under 20X and 60X ...
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bioRxiv - Microbiology 2021Quote: ... antibody solutions were diluted in PBS with 0.1% saponin at a 1:10 dilution for mouse α-LAMP-1 (ab25630, Abcam, Cambridge, UK), 1:50 for rabbit α-EEA1-Alexa Fluor 647 (ab196186 ...
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bioRxiv - Immunology 2021Quote: ... Concentration of circulating LPS-binding protein (LBP) was determined in plasma samples (diluted 1:5) using Mouse LBP ELISA Kit (Abcam, Cambridge, MA) according to manufacturer’s instructions ...
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bioRxiv - Cell Biology 2021Quote: ... The pellet was re-suspended with 0.9% of sodium chloride solution and reacted with a rabbit anti-mouse HMGB1 primary antibody for 3 hrs at room temperature (1:350, abcam, Cat. #ab18256), followed by a goat anti-rabbit secondary antibody conjugated with Cy3 for 1hr at room temperature (1:500 ...
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bioRxiv - Microbiology 2021Quote: ... was used for indirect immunofluorescence microscopy at a dilution of 1:300 and mouse monoclonal antibody against nuclear pore complex proteins (Abcam, Cambridge, MA) was used for indirect immunofluorescence microscopy at a dilution of 1:500 ...
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bioRxiv - Cell Biology 2021Quote: ... lungs were incubated with conjugated fluorescent secondary Alexa Fluor 555 donkey anti-mouse IgG (H+L) (Abcam; Material No. ab150106; 1:250) for two days at 4 °C.
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bioRxiv - Neuroscience 2021Quote: ... were subjected to western blotting using the indicated primary (mouse anti-GABAA receptor alpha 1 #ab3299, Abcam, 1:200; rabbit antibeta actin #ab8227, Abcam, 1:1000) and secondary antibodies ...
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bioRxiv - Microbiology 2022Quote: ... Secondary antibodies used for western blotting included HRP-conjugated goat anti-Rabbit and sheep anti-Mouse antibodies (Abcam, ab205718 and ab6808, respectively).
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bioRxiv - Developmental Biology 2022Quote: ... for 1 h at RT and directly labelled overnight at 4°C with primary antibodies (1:400 mouse mAB for sarcomeric alpha-actinin [CloneEA-53, Sigma-Aldrich, A7811] and 1:200 rabbit pAB for cardiac Troponin T [Abcam, ab45932]). After washing cells once with PBS ...
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bioRxiv - Immunology 2022Quote: ... Slides containing 4 sections per slide and 8 sections per sample were incubated with primary antibodies at 4°C overnight and then washed three times with PBS before incubating them with goat anti-mouse IgG (H&L) secondary antibody (Alexa Fluor® 488 in 1% BSA PBST, 1:300; Abcam #ab150113) for 1 h at room temperature in the dark ...
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bioRxiv - Cell Biology 2022Quote: ... The membrane was washed in TBST and incubated for 45 min in TBST containing 4% dried milk and HRP mouse anti-beta Actin AC-15 (1:25000, abcam Ref. ab49900). The membrane was washed in TBST and incubated 1 min in ECL Western Blotting Substrate (Thermo Fisher Scientific Ref ...
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bioRxiv - Genetics 2022Quote: ... The membrane was blocked with 5% milk in TBST (B1009, Applygen, Beijing, China) and incubated with a primary mouse monoclonal 1D4(1:1000 dilution, ab5417, Abcam, Cambridge, MA)) ...
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bioRxiv - Neuroscience 2022Quote: ... Cells were incubated overnight at 4 °C with primary antibodies — targeting α-syn (Mouse anti α-syn, AA 124-134, ab1903, Abcam; Mouse anti α-syn 4B12 ...
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bioRxiv - Physiology 2023Quote: ... and the anti-alpha 1 Na+/K+-ATPase mouse monoclonal antibody (Na+/K+-ATPase α1, #ab7671, Abcam, Cambridge, United Kingdom, 1:1000) were used to verify equal protein loading ...
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bioRxiv - Physiology 2022Quote: ... Total protein levels were measured by BCA assay (Pierce) and a 1:00 dilution was used for quantifying α-synuclein using the mouse α-synuclein ELISA kit (Abcam) following the manufacturer’s instructions.
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bioRxiv - Genetics 2022Quote: ... 1:1000; Anti-GAPDH mouse, Abcam ab8245, 1:10,000; Anti-nitrotyrosine, Merck 05-233, 1:100; Anti-GAPDH rabbit, Abcam ab9485, 1:1000), 3x 5-min washes with PBS-T ...
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bioRxiv - Developmental Biology 2022Quote: ... 1:250 mouse anti-beta tubulin (E7; Developmental Studies Hybridoma Bank, Iowa City, IA) 1:250 rabbit anti-histone H3 (ab1791; Abcam, Cambridge, MA), 1:1000 Anti-phospho-Histone-H3(ser10 ...
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bioRxiv - Cancer Biology 2024Quote: ... Samples were then incubated overnight with the following primary antibodies diluted in PBS with 0.1% Tween and 1% BSA: rabbit anti-mouse RPA2 (Abcam; ab76420; 1:50), rat anti-mouse RPA2 (CST ...
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bioRxiv - Biochemistry 2024Quote: ... The cell lysates were boiled in SDS-PAGE loading buffer for 10 min and then subjected to SDS-PAGE and probed with the following specific antibodies: mouse anti-TDP-43 monoclonal antibody (Abcam, ab57105,1:1000), rabbit anti-TDP-43 polyclonal antibody (Proteintech ...
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bioRxiv - Cell Biology 2024Quote: ... for 30 min at room temperature and incubated with the primary antibody against mouse UCP1 (ab234430, Abcam, UK; diluted at 1:500) at 4°C for 12 h ...
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bioRxiv - Neuroscience 2024Quote: ... sections were incubated overnight at 4 ℃ in an antibody diluent (1.1% Triton-X and 2% normal goat serum in PBS) containing primary antibodies anti-TUBB3 (mouse, 1:1000, Sigma-Aldrich, T8660; rabbit, 1:300, Abcam, ab18207), anti-SOX2 (rabbit ...
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bioRxiv - Neuroscience 2024Quote: ... Triton X-100 in PBS and incubated with donkey-anti-mouse IgG (H+L) Alexa Fluor 488 nm (1:500, Abcam, UK, ab150109), donkey-anti-rabbit IgG (H+L ...
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bioRxiv - Immunology 2024Quote: ... samples were treated with unconjugated affinity purified F(ab) fragment IgG anti-mouse (H+L) for 1 hr at RT (0.1 mg/ml, Abcam, cat no. ab6668). Mouse epithelium was stained with Alexa 633-conjugated phalloidin (Invitrogen ...
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bioRxiv - Immunology 2023Quote: ... The plates were washed three times with PBST and then incubated with anti-mouse IgG or IgA secondary antibody conjugated to horseradish peroxidase (HRP) (Abcam, Cambridge, UK) in PBST for 1 h at room temperature ...
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bioRxiv - Cell Biology 2023Quote: ... Immuno-staining was conducted using mouse anti-β-tubulin 1E1-E8-H4 antibody (dilution 1:300, cat. nr. ab131205, Abcam, Cambridge, UK), or goat anti-collagen Iα1 antibody (dilution 1:20 ...
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bioRxiv - Developmental Biology 2023Quote: ... The following primary antibodies were used: anti-SOX2 diluted 1:500 (R&D AB2018, mouse) and anti-NR2F1 diluted 1:1000 (Abcam Ab181137, rabbit). Sections were then washed 2X for 10 minutes in PBS1X and incubated for 1h at room temperature with blocking solution containing the following fluorescent secondary antibodies ...
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bioRxiv - Molecular Biology 2022Quote: ... Ectopic huLiver tissue was processed and stained using the methods described above with polyclonal rabbit anti-MSP1 (1:100) and monoclonal mouse anti-EBA-175 (1:100) followed with goat anti-rabbit IgG (1:100) (Abcam, Cambridge, UK), and goat α-mouse IgG1 (1:100 ...
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bioRxiv - Molecular Biology 2022Quote: ... blood samples from each repopulated mouse were collected via the saphenous vein and analyzed using the Human Albumin ELISA Quantitation Set (Abcam, Cambridge, UK) according to the manufacture’s protocol ...
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bioRxiv - Microbiology 2022Quote: Immunohistochemistry was performed on formalin-fixed cryosections of mouse aortic roots using antibodies to identify macrophages (MOMA-2, 1:50; ab33451, Abcam, Cambridge, MA), followed by detection with biotinylated secondary antibodies (1:400 ...
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Mucociliary Clearance Augmenting Drugs Block SARS-Cov-2 Replication in Human Airway Epithelial CellsbioRxiv - Microbiology 2023Quote: ... sections corresponding to tubulin staining were incubated with a goat anti-mouse IgG secondary antibody conjugated with HRP (Abcam ab6789, 1:500), while sections of caspase and protein S staining were incubated with goat anti-rabbit IgG secondary antibody conjugated with HRP (Abcam ab6721 ...
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bioRxiv - Synthetic Biology 2023Quote: ... The membrane was washed with TBS-T and incubated for 1 hour at room temperature with a 1:10,000 dilution of HRP-conjugated goat anti-mouse secondary antibodies (Abcam ab6789-1 MG) in 5% milk-TBS-T ...
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bioRxiv - Cancer Biology 2023Quote: ... The membranes were washed with TBST and incubated with the HRP-conjugated secondary antibodies (Goat pAb to Rabbit and Mouse IgG, Abcam, ab205718, ab205719) for 1 h at rt ...
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bioRxiv - Neuroscience 2023Quote: ... cells were incubated with primary antibodies diluted in blocking buffer overnight at 4°C - Mouse Anti-Human TRA1-81 (1:75, Abcam, AB16289#20), Rabbit Anti-Human OCT4 (1:250 ...
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bioRxiv - Physiology 2023Quote: ... Adjacent sections were used for immunohistochemistry with anti-VDR (1:100, rat) and anti-AMH (1:50, mouse) monoclonal antibodies (Abcam, Cambridge, UK) as per manufacturers’ protocol with modifications.
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bioRxiv - Physiology 2023Quote: ... with the primary antibody goat anti-HIF-1α (1:100, SicGen, Cantanhede, Portugal) and mouse anti-HIF-2α (1:100, Abcam, Cambridge, UK). After washing the slices were incubated with secondary antibody donkey anti-goat Alexa Fluor 546 (1:5000 ...
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bioRxiv - Physiology 2023Quote: ... Solution was decanted and slices washed in TBS-T before being incubated with specific secondary antibody in a dilution of 1:2000 for 1h at 24°C in the dark (anti-rabbit Alexa Fluor 550 or anti-mouse Alexa Fluor 488; from Abcam, Cambridge, UK). Slices were washed in TBS in the dark and mounted with a coverslip and a drop of mounting medium Fluoroshield™ - Sigma-Aldrich ...
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bioRxiv - Genomics 2023Quote: ... cells were blocked for 1 h in 1×PBS + 2% w/v BSA and then incubated with mouse anti-E-cadherin (1:1000) (Abcam, cat#231303) and chicken anti-vimentin (1:200 ...
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bioRxiv - Biochemistry 2023Quote: The following secondary antibodies have been used for Western blotting: Goat Anti-Mouse IgG H&L (HRP) preadsorbed (Abcam, #ab7068; 1:10000), Recombinant Protein G (HRP ...
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bioRxiv - Plant Biology 2023Quote: ... and incubated for 2 h with 700 µL of a 1/250 dilution of anti-mouse IgG Alexa FluorTM 555 secondary antibody (ab150106; Abcam, Cambridge, UK) in MTSB + 5% (v/v ...
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bioRxiv - Neuroscience 2023Quote: ... The macrophages in the atherosclerosis in the thoracoabdominal aorta of the rabbits were revealed using mouse anti-rabbit CD68 antibody (Abcam, Cambridge, UK) as the primary antibody and goat anti-mouse IgG antibody (horseradish peroxidase (HRP ...
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bioRxiv - Cell Biology 2022Quote: ... Rabbit monoclonal IgG antibodies against MMP-9 and Cx43 and CD31 and a mouse IgG antibody against Rac-1 were purchased from Abcam (Cambridge, UK). Rabbit polyclonal IgG antibodies against Na+/K+-ATPase and CD68 were purchased from Arigo (Hsinchu ...
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bioRxiv - Cell Biology 2023Quote: ... cells were stained for 30 min with polyclonal fluorescein-isothiocyanate (FITC)-conjugated goat anti-mouse IgG H&L specific antibody (Abcam, United Kingdom) diluted 1:50 in RS-BSA 0.1% ...
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bioRxiv - Genetics 2023Quote: ... The chromatin was purified through centrifugation and immunoprecipitated using Dynabeads M-280 Sheep anti-mouse IgG (Veritas Life Sciences, PA, USA) conjugated with anti-H3K36me2 antibodies (ab176921; Abcam, Cambridge, UK) in 1× RIPA (150 mM ...
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bioRxiv - Immunology 2023Quote: ... The following primary antibodies were used for the western blot experiments: mouse monoclonal antibody against human apolipoprotein E (dilution 1:1000; Abcam, Cambridge, UK), and polyclonal rabbit antibodies against the LL37 epitope (diluted 1:1000 ...
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bioRxiv - Molecular Biology 2023Quote: ... mouse monoclonal Abs (mMAbs) against lamin A/C C-terminus residues aa 319-566 (LA/C-C, [131C3], ab18984, Abcam, Cambridge, UK) and lamin A/C N-terminus residues aa 2-29 (LA/C-N ...
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bioRxiv - Biochemistry 2023Quote: ... The grids were then washed with PBS+ three times for 2 min and incubated on a 20 ul drop of secondary antibodies conjugated with gold particles (1:20 dilution of anti-mouse and anti-rabbit secondary antibodies conjugated with 10nm and 6nm gold particles respectively, Abcam, Cambridge, UK). Finally ...