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Citations for abcam :
4351 - 4400 of 4741 citations for PLGF Mouse HEK 293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2020Quote: ... Tissue was then incubated for 2 hours at room temperature in a PBTGS secondary antibody solution including goat anti-mouse Alexa568 (1:200; Abcam [ab175473]), goat anti-rabbit Cy2 (1:200 ...
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bioRxiv - Microbiology 2021Quote: ... then incubated overnight at 4°C with the primary antibody mouse anti-SA Hla [8B7] -N-terminal (Abcam, Cambridge, United Kingdom) diluted 1:2500 in the blocking buffer ...
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bioRxiv - Immunology 2021Quote: ... staining with primary and secondary antibodies were realized according to the manufactures of the following IHC kits: Mouse and rabbit specific HRP/DAB IHC detection Kit micro-polymer (Abcam, Cat.ab236466) and ImmPRESS[R] Duet double staining kit anti-rabbit AP/anti-mouse HRP (Vector laboratories ...
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bioRxiv - Cancer Biology 2021Quote: ... a pre-diluted mouse monoclonal anti-p53 (Clone Bp53-11, Ventana Medical Systems, Inc.) and a rabbit polyclonal anti-CDC20 (Abcam #ab72202) at 1:300 were manually applied for 32 min at 37°C ...
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bioRxiv - Neuroscience 2020Quote: ... followed by a 2 hr incubation with a mixture of the secondary antibody (goat anti-mouse Alexa Fluor® 488–conjugated IgG, ab150113 Abcam) and Alexa Fluor 568 conjugated Phalloidin (A12380 ...
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bioRxiv - Bioengineering 2020Quote: ... Richard-Allan Scientific) and α-smooth muscle actin (α-SMA) (primary Rabbit anti-mouse alpha-SMA antibody; Cat. No. ab5694; Abcam; 1:50 dilution ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were washed and incubated in darkness with goat anti-mouse secondary antibodies (1:500, Alexa fluor 488, Abcam, Cambridge, UK) in 0.1% Tx-100 ...
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bioRxiv - Molecular Biology 2021Quote: ... cells were permeabilized using 0.1% Triton-X and incubated for 2 hours at room temperature with mouse monoclonal antibody against sarcomeric α-actinin (1:100; Abcam), followed by an incubation of 1 hour with the secondary antibody conjugated to Alexa Fluor-488 (Life Technologies) ...
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bioRxiv - Immunology 2021Quote: ... Fixed cells were blocked with 5% BSA in PBS for 1 hr at room temperature and incubated with rabbit anti-mouse Ki67 primary antibody (Abcam ab15580) at 1:200 dilution overnight at 4°C ...
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bioRxiv - Cell Biology 2021Quote: ... and incubated at RT for 2 h with mouse anti-CTLA-4 monoclonal antibody (diluted: 1:3 000; Abcam, Cambridge, UK) as the primary antibody ...
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bioRxiv - Biophysics 2020Quote: ... the samples were incubated with the appropriate secondary antibody (polyclonal goat anti-mouse IgG H&L, 1:1000, Abcam, Cambridge, UK) diluted in blocking solution for 45 min at room temperature ...
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bioRxiv - Cell Biology 2021Quote: ... cells were incubated 45 min at room temperature with the respective secondary antibodies: anti-mouse Alexa-Fluor® 594 (abcam, ab150108), anti-chicken Alexa-Fluor® 633 (Sigma ...
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bioRxiv - Cell Biology 2021Quote: ... slides were thoroughly washed using PBS 1X/Triton- X 0.1% and incubated overnight at 4°C with the respective antibodies anti-mouse Alexa-Fluor® 594 (abcam, ab150108), anti-sheep Alexa-Fluor® 488 (Jackson Immunoresearch ...
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bioRxiv - Cell Biology 2022Quote: ... The coverslips were incubated with the primary antibodies (rabbit anti-TPR, 1:100, NB100-2867; and mouse anti-NUPs, 1:100, Abcam 24609) in blocking solution overnight at 4°C ...
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bioRxiv - Biophysics 2022Quote: ... Membranes were washed 3 times for 10 minutes at room temperature with TBST and incubated with either anti-Mouse IgG-HRP (1:20 000, Abcam, ab6728) or anti-Rabbit IgG-HRP (1:20 000 ...
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bioRxiv - Bioengineering 2022Quote: ... samples were washed x3 times with PBS and incubated for 2 h at room temperature with corresponding secondary antibody (α-mouse. 488, 1:500, ab150077, abcam; α-rabbit ...
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bioRxiv - Bioengineering 2022Quote: ... samples were washed x3 times with PBS and incubated for 2 h at room temperature with corresponding secondary antibody (α-mouse. 488, 1:500, ab150077, abcam; α-rabbit. 488,1:500, ab150077, abcam; α-goat ...
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bioRxiv - Developmental Biology 2022Quote: ... and rabbit polyclonal anti-RAD51 (1:1000, (75)), anti-SYCP1 (1:2500, (76)) and mouse monoclonal anti-DMC1 (1:1000, Abcam, ab11054), anti-SYCP3(1:200 ...
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bioRxiv - Cell Biology 2022Quote: ... Primary antibodies were rabbit anti-SEPT7 (1:400, IBL 18991) and mouse anti-non-muscle Myosin IIA (1:200, abcam ab55456). Secondary antibodies were goat AlexaFluor488-conjugated anti-mouse IgGs (1:400 ...
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bioRxiv - Microbiology 2022Quote: ... Spain) binding to the 229E nucleocapsid protein and a goat anti-mouse secondary antibody conjugated with fluorescein isothiocyanate (Abcam, United Kingdom). TCID50 titers were calculated according to the method of Reed and Muench (44).
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bioRxiv - Neuroscience 2022Quote: ... and validated primary antibodies: mouse anti-CaVβ4 (Neuromab #75-054, 1:1000) and rabbit anti-β-tubulin (Abcam #ab6046, 1:600,000). The membrane was then incubated in LiCor Blocking Buffer diluted 1:1 with TBS (0.1% Tween 20 and 0.02% SDS ...
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bioRxiv - Microbiology 2022Quote: ... Slides were washed once again and HRP-conjugated secondary antibody was added at a 1:5000 concentration (Goat anti-mouse: ThermoFisher, Cat #A21426; Goat anti-rabbit: Abcam, Ab6721). Slides were washed twice prior to application of DAB developing reagent (Vector Laboratories ...
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bioRxiv - Bioengineering 2022Quote: ... ECM components were detected using mouse mAbs specific for collagen type I (0.5 μg/ml clone ab90395, Abcam®, Cambridge, UK), collagen type IV (2 μg /ml clone M0785 ...
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bioRxiv - Cell Biology 2022Quote: Epidermal punch biopsies (6 mm diameter) in triplicates were floated on medium containing either a mouse α-RTN1A ab (mon162, abcam) or the respective isotype control ab (IgG1 ...
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bioRxiv - Cancer Biology 2022Quote: ... Coverslips were then washed with 1xPBS 3x for 5 minutes before incubation in 50 µl spots of secondary antibodies (goat anti-mouse AF647: Abcam ab150115, goat anti-rabbit AF405: abcam 175652) diluted in incubation buffer for 1hr at RT ...
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bioRxiv - Microbiology 2021Quote: ... horseradish peroxidase (HRP)-conjugated goat anti-mouse antibodies against the light chain (Fab) and heavy chain (Fc) (Abcam,plc, Cambridge, MA), and HRP-conjugated goat anti-rabbit antibody (Abcam ...
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bioRxiv - Neuroscience 2021Quote: ... and then incubated in secondary antibodies (donkey antichicken Alexa 488, Jackson ImmunoResearch 703-545-155; donkey anti-mouse Dylight 550, Abcam ab96876 ...
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bioRxiv - Immunology 2020Quote: Transferrin and cytokines in the samples (cell supernatant or plasma) were measured using a mouse transferrin ELISA kit (ab157724, Abcam, USA), mouse TNF-α ELISA kit (DKW12-2720-096 ...
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bioRxiv - Neuroscience 2021Quote: ... 0.1% SDS) and incubated overnight at 4°C with 3 μL specific mouse monoclonal antibody recognizing Bcl11a (Abcam Cat# ab19487, RRID:AB_444947) or unspecific IgG1 antibody (Cell Signaling Technology Cat# 5415 ...
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bioRxiv - Microbiology 2021Quote: ... the samples were incubated with goat anti-mouse IgG Alexa Fluor 488 secondary antibody (1:1000 dilution; Abcam, Cambridge, MA, USA) for 1 h at 25℃ and the slides were mounted in antifade Mounting Medium with DAPI (Vectashield ...
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bioRxiv - Developmental Biology 2022Quote: Immunofluorescence was performed on serial sections along the rostral–caudal brain axis using the following antibodies: mouse monoclonal anti-GLUT1 (Abcam, ab40084), chicken polyclonal anti-GFP (Abcam ...
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bioRxiv - Cancer Biology 2022Quote: ... B16F10 cells were stained with a 1:10 dilution of individual animal serum followed by detection with a 1:1000 dilution of a goat anti-mouse Fc FITC secondary (Abcam ab98716) and fixation with 4% formalin ...
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bioRxiv - Cell Biology 2022Quote: ... and incubated with the indicated primary antibodies diluted in blocking buffer at 4°C overnight: mouse-pH3 (1:2000, Abcam, ab14955), rabbit-Ki67 (1:500 ...
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bioRxiv - Neuroscience 2022Quote: ... sections were incubated at 4°C overnight in a solution of mouse anti-c-fos primary antibody (1:1000 dilution, Abcam, UK) or mouse anti-TPH2 primary antibody (1:100 dilution ...
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bioRxiv - Cell Biology 2019Quote: ... santacruz sc293), CDK7 (1/2000, rabbit, santacruz sc856X), CyclinH (1/1000, mouse, IGBMC 2D4) and p62 (1/500, rabbit, abcam ab204168). The loading was controlled with anti-α-tubulin antibody (1/50000 ...
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bioRxiv - Developmental Biology 2019Quote: ... After washing 3 times with TBS-T the blots were incubated with HRP-conjugated secondary antibodies (goat-anti-mouse HRP (Abcam, ab6789); goat-anti-rabbit HRP (Abcam ...
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bioRxiv - Neuroscience 2020Quote: ... Sections were then washed in PBS (3×10 min) and incubated for 2h in secondary antibody diluted in blocking solution (1:1000 Goat-α-mouse AlexaFluor488, abcam ab150113 ...
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bioRxiv - Cancer Biology 2019Quote: ... Whole pancreas protein extracts were isolated and incubated with mouse inflammation/cytokine antibody arrays (40 targets, Abcam, ab133999; 97 targets, Abcam ab169820), according to manufacturer’s instructions ...
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bioRxiv - Bioengineering 2019Quote: ... followed by overnight staining with 1:300 dilutions of rat-anti-C-peptide (DSHB; GN-ID4-S) and mouse-anti-glucagon (ABCAM; ab82270) primary antibodies ...
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bioRxiv - Microbiology 2019Quote: ... After blocking, the cells were stained with Mouse Anti-Human Myeloperoxidase (Santacruz, Germany) and Rabbit Anti-Human Histone-H3 (Abcam, USA) antibodies for one hour ...
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bioRxiv - Systems Biology 2020Quote: ... cells were stained at 4°C o/n in 50 µL of the 1st antibody diluent (rabbit anti-ZO-1, 61-7300, Thermo Fisher Scientific Inc., MA, USA; anti-MITF, mouse anti-MiTF, ab80651, Abcam plc. ...
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bioRxiv - Developmental Biology 2020Quote: ... mouse anti-Laminin (1:500, DSHB 3H11), mouse anti-Msx1/2 (1:100, DSHB 4G1) and rabbit anti-RFP (1:500, Abcam ab62341).
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bioRxiv - Bioengineering 2021Quote: ... diluted at 1:100 followed by 2 h of incubation with 1:500 AlexaFluor 568 goat anti-mouse IgG (abcam, UK) diluted at 1:500 ...
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bioRxiv - Immunology 2019Quote: ELISAs were performed using Mouse MIP-1 alpha (CCL3) ELISA Ready-SET-Go ELISA kit (eBi-oscience™) and Prostaglandin E2 ELISA Kit (Abcam) according to the manufacturer’s instructions ...
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bioRxiv - Synthetic Biology 2020Quote: ... The following antibodies were used: mouse anti-CENP-A (clone A1, 1:500,36) and rabbit anti-H3K9me3 (abcam 8898; 1:200). Microscope images were acquired on a DeltaVision Core system (Applied Precision ...
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bioRxiv - Cancer Biology 2019Quote: ... slides were incubated for 2.5h with primary Abs for (IdU: 1:100, mouse monoclonal anti-BrdU, Becton Dickinson 347580; CldU: 1:100, rat monoclonal anti-BrdU, Abcam 6326) diluted in blocking buffer ...
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bioRxiv - Neuroscience 2020Quote: ... the blot was incubated with 1:1000 diluted buffer of secondary rabbit anti-mouse HRP conjugated antibody (Cat # 6728, Abcam, UK) in 1X PBS for 1 h at room temperature ...
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bioRxiv - Molecular Biology 2020Quote: ... proteins were blocked with 1% normal goat serum (30 min at room temperature) followed by a goat anti-mouse IgG2a antibody (Abcam, ab98694). After additional blocking with normal goat serum ...
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bioRxiv - Immunology 2021Quote: ... For detection of IgG subclasses all serum samples were diluted to 1 total IgG EU and incubated at 37 °C for 1 h prior to detection with Alkaline Phosphatase conjugated anti-mouse IgG subclass-specific secondary antibodies (Southern Biotech or Abcam) incubated for 1 h at 37 °C ...
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bioRxiv - Cancer Biology 2020Quote: ... The following primary antibodies and their respective dilutions were used in immunofluorescence (IF) and Western blot (WB) experiments: MITF (C5) mouse monoclonal (Abcam, #ab12039), 1:2000 (WB) ...