Labshake search
Citations for abcam :
351 - 400 of 638 citations for 5M Betaine Solution PCR Grade since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Molecular Biology 2024Quote: ... cells were washed with PBS and added 100 μl media with 10% of CCK-8 solution (Ab228554, Abcam). The plate was then incubated in a light-restricted setting at 37°C for 2 hours ...
-
bioRxiv - Cell Biology 2024Quote: ... Sections for H&E were fixed with 4% paraformaldehyde (PFA) and stained with Mayer’s modified hematoxylin solution (Abcam) and washed for five minutes followed by differentiation with 1% HCl in 10% ethanol ...
-
bioRxiv - Genomics 2023Quote: ... Cells were regularly tested for mycoplasma contamination using the Mycoplasma PCR Detection Kit (abcam ab289834). E14 mouse embryonic stem cells were a gift from W ...
-
bioRxiv - Biochemistry 2023Quote: ... Genomic PCR and western blot (anti-DUS1L, Proteintech, 1:1000; anti-DUS2L, Abcam, 1:1000) were performed to confirm KO.
-
bioRxiv - Immunology 2021Quote: ... slides were rinsed with PBS-0.05% Tween-20 solution and then incubated with primary antibodies for HLA-DR to label MNPs (Abcam, Cambridge ...
-
bioRxiv - Neuroscience 2022Quote: ... Sections were incubated overnight at 4°C in a solution containing the antibodies NeuN rabbit (ab104225, 1:1000, Abcam). After additional washes ...
-
bioRxiv - Bioengineering 2021Quote: ... 1 hr) with antibody solution (2.5% BSA and 0.05% sodium azide in PBS) before blotted with anti-Securin (Abcam; #AB3305) primary antibody (RT ...
-
bioRxiv - Synthetic Biology 2021Quote: ... This was followed by 10 mins of DAPI Staining Solution in PBS (1:1000, ab228549, Abcam, Cambridge, MA, USA) after which point slides were cover-slipped with ProLong Gold Antifade Mountant (Thermo Fisher Scientific ...
-
bioRxiv - Neuroscience 2021Quote: ... A subset of sections was mounted onto glass slides and stained with a 1% Nissl solution (ab246817; Abcam, USA), and then were coverslipped with mounting medium (Permount) ...
-
bioRxiv - Physiology 2020Quote: ... rinsed in PBS 2×10min and then incubated in blocking solution containing 5% normal goat serum (NGS, Ab7481, Abcam), 0.5% Triton (T8787 ...
-
bioRxiv - Neuroscience 2020Quote: ... and incubated overnight in primary antibody diluted in blocking solution containing Triton-X (0.4%): chicken anti-YFP (1:100, Abcam), rabbit anti-NG2 (1:500 ...
-
bioRxiv - Cancer Biology 2022Quote: ... blocked with the blocking solution (Duolink) for 1 h and incubated overnight with anti-PCNA (1:5,000, ab 92552 (AbCam)) and anti-phospho-RNA polymerase II (S5 ...
-
bioRxiv - Neuroscience 2021Quote: ... membranes were incubated in block solution overnight with the following primary antibodies: rabbit anti-lamin-B1 (1:1,000; Abcam) and mouse anti-GAPDH (1:1,000 ...
-
bioRxiv - Neuroscience 2021Quote: ... the baseline was recorded for 1 min before applying 4 µM FCCP (Abcam, 20 mM stock solution in DMSO) for 2 min ...
-
bioRxiv - Neuroscience 2021Quote: ... and incubated overnight at 4° C with a solution containing 5% goat serum (GS) and anti-cFos (rabbit; 1:5000, AB_190289, Abcam) diluted in PBST ...
-
bioRxiv - Bioengineering 2020Quote: ... the membrane was incubated with freshly prepared primary antibody solution (1:1,000 dilution for anti-Alix [ab117600, Abcam], anti-Tsg101 [ab30871, Abcam] ...
-
bioRxiv - Biochemistry 2021Quote: ... Secondary antibody solution containing 1:3000 horseradish peroxidase (HRP) conjugated goat anti-mouse secondary antibody (Abcam ab6789-1 MG) in PBS-T was added to each well and incubated for 1 hour ...
-
bioRxiv - Neuroscience 2021Quote: ... sections were incubated for 1 hour in a blocking solution comprising PBS with 0.3% (weight/volume) Triton X-100 and 5% (weight/volume) donkey serum (Abcam) containing 1% (w/v ...
-
Empowering Engineered Muscle Function by Extending Connexin 43 Duration with Reduced Graphene OxidesbioRxiv - Bioengineering 2021Quote: ... The University of Iowa Department of Biology) or a solution of anti-sarcomeric α-actinin antibody (Abcam, Cambridge, U.K.). On the next day ...
-
bioRxiv - Molecular Biology 2020Quote: ... Samples were blocked at RT for 30 min in blocking solution (1x PBS3T 5% normal goat serum (Abcam ab138478)) and incubated in primary antibody dilution buffer (antibody diluted in 1x PBS3T and 1% BSA ...
-
bioRxiv - Bioengineering 2020Quote: ... the membrane was incubated with freshly prepared primary antibody solution (1:1,000 dilution for anti-Alix [ab117600, Abcam], anti-Tsg101 [ab30871, Abcam], anti-Calnexin [ab22595, Abcam] ...
-
bioRxiv - Cancer Biology 2022Quote: ... Sections were incubated at 4°C overnight with LRP1 primary antibody diluted in blocking solution (1:100; abcam 92544). Slides were washed with PBS and then incubated with Alexa Fluor 488 anti-rabbit secondary antibody diluted in PBST for 45 minutes (1:200 ...
-
bioRxiv - Cell Biology 2022Quote: ... The lentivirus was collected by centrifugation and the pellet was suspended in Viral Re-suspension Solution (Abcam, Cambridge, UK). Additional details of spike viral pseudoparticle are in the Supplement.
-
bioRxiv - Cell Biology 2022Quote: ... The lentivirus was collected by centrifugation and resuspension of the pellet in Viral Re-suspension Solution (Abcam, Cambridge, UK). The virus aliquots were stored at −80°C ...
-
bioRxiv - Physiology 2023Quote: ... in DPBS for 1 h at room temperature followed by incubation in blocking solution (2% donkey serum (#ab7475, abcam), 3% BSA (#A7030 ...
-
bioRxiv - Neuroscience 2022Quote: ... Then the sections were incubated with primary antibody of interest in the blockage solution (c-fos,1:200, Abcam/ab208942 ...
-
bioRxiv - Neuroscience 2022Quote: ... Primary antibodies were diluted in blocking solution and incubated as follow: rabbit polyclonal α-CgA (1:500; Abcam 15160) and mouse monoclonal α-TH (1:2000 ...
-
bioRxiv - Biophysics 2024Quote: ... Attached cells were then fixed by methanol/acetic acid solution (3:1) stained with Perl’s Prussian blue with nuclear fast red as counterstain (Iron stain kit, ab150674, abcam). Slides were dehydrated (incubation with 75 ...
-
bioRxiv - Systems Biology 2023Quote: ... after which the block/perm solution was removed and replaced with DPBS supplemented with Phalloidin iFlouor 555 (Abcam #ab176756) and HCS CellMask Blue (Thermo Fisher Scientific #H32720) ...
-
bioRxiv - Neuroscience 2023Quote: ... sections were incubated with PBST (Triton 0.3 %) solution containing 5 % donkey normal serum and one or more antibodies (goat Anti GFP, ab6673, Abcam, diluted 1:2000 ...
-
bioRxiv - Neuroscience 2024Quote: ... All chambers were again washed twice with PBS before a blocking solution consisting of 5 % goat serum (Abcam, ab7481) diluted in PBS was added to the cultures ...
-
bioRxiv - Bioengineering 2023Quote: ... separate slides were incubated in a blocking solution containing 0.3% Triton X-100 and 10% normal goat serum (Abcam) in 1xPBS + 100 mM CaCl2 + 100 mM MgCl ...
-
bioRxiv - Immunology 2022Quote: ... the sections were incubated at 4 °C overnight with the primary rabbit polyclonal anti-tropoelastin antibody solution (Abcam, ab21600) 1:200 in 0.5% BSA in TBS ...
-
bioRxiv - Pathology 2023Quote: ... and then incubated for 1 hour in PB solution containing 5% normal goat serum (Abcam Cat# ab7481, RRID: AB_2716553). The sections were then incubated with the primary antibody ...
-
bioRxiv - Neuroscience 2023Quote: ... slices were washed thoroughly in PBST and incubated in a solution of Alexa Fluor 647-conjugated anti-rabbit (1:1,000; Abcam) and either Alexa Fluor 488 conjugated anti-guinea pig antibody (1:500 ...
-
bioRxiv - Microbiology 2023Quote: ... Membranes were washed in wash buffer (PBS with 0.05% Tween) and incubated in blocking solution with rabbit anti-sheep IgG (Abcam) at 1:40,000 dilution for 1 hour at room temperature and visualised on LiCor Odyssey Fc (Invitrogen).
-
bioRxiv - Neuroscience 2023Quote: ... and 0.2% Triton X-100 for 1 h at room temperature followed by an overnight incubation at 4°C with primary antibody solution containing a rabbit anti-c-Fos (1:1,000; #ab222699, Abcam) antibody in PBS with 1% NDS and 0.2% Triton X-100 ...
-
bioRxiv - Biochemistry 2023Quote: ... cells were incubated with a solution of primary antibodies (mouse anti α-tubulin, Abcam, ab7291 or rabbit anti-TOM20) in staining buffer (PBS ...
-
bioRxiv - Synthetic Biology 2023Quote: ... the membrane was incubated overnight at 4°C with primary antibody solution (1:1000 dilution for anti-TSG101 [Abcam, ab30871] ...
-
bioRxiv - Neuroscience 2023Quote: ... Deparaffinized and rehydrated slides were submerged in 1X antigen retrieval solution (100X Tris-EDTA Buffer, pH 9.0, ab93684, Abcam) and placed in a tissue steamer (IHC-Tek Epitope Retrieval Steamer Set ...
-
bioRxiv - Cell Biology 2023Quote: MTS assay was performed as previously described.21 Individual PCLS were placed in a 24-well plate with 400 μL William’s E medium and 80 μL MTS solution (Abcam) for each well ...
-
bioRxiv - Neuroscience 2024Quote: ... All slices were incubated overnight at 4 °C in blocking solution containing either 1:1000 anti-GFP (ab13970, Abcam) to reveal dLight1.1-expressing cells in NAc ...
-
bioRxiv - Neuroscience 2024Quote: ... and D-AP5 (final concentration 100 µM; 1 μL stock solution in water in 1 mL media; ab120003; Abcam) and incubated for 80-90 minutes at 37°C in humidified 5% CO2 ...
-
bioRxiv - Neuroscience 2024Quote: ... the sections were incubated overnight with primary antibodies diluted in blocking solution: rabbit anti-foxP2 (1:2000; Abcam, ab16046), rabbit anti-red fluorescent protein (anti-RFP ...
-
bioRxiv - Neuroscience 2024Quote: ... For K+ currents (IK) the bath solution above was supplemented with 1 μM TTX (Tetrodotoxin Citrate, Abcam (Melbourne, Australia)) ...
-
Spatial 3D genome organization controls the activity of bivalent chromatin during human neurogenesisbioRxiv - Neuroscience 2024Quote: ... Cells were incubated in antibody binding solution (5% NGS and 0.1% Triton X-100 in D-PBS) containing LaminB1 (Abcam) or SON (Atlas Antibodies ...
-
bioRxiv - Neuroscience 2024Quote: ... the sections were exposed to a secondary antibody solution containing the secondary antibody of interest (1:500; #ab150173, abcam; #A21206 ...
-
bioRxiv - Neuroscience 2024Quote: ... The cells were then incubated with the primary antibodies diluted in blocking solution (FTO (mouse, Abcam – ab92821, 1:1000), METTL3 (rabbit ...
-
bioRxiv - Genomics 2024Quote: ... cells were incubated with a solution of D-Wash buffer and Rabbit-anti-mouse IgG (1:200, Abcam, ab6709), followed by a wash step ...
-
bioRxiv - Cancer Biology 2021Quote: ... in PBST at room temperature was followed by incubation with primary antibody for CD163 (Abcam, ab182422, 1.4ug/ml working solution) at 4°C overnight ...