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Citations for abcam :
601 - 638 of 638 citations for 5M Betaine Solution PCR Grade since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2024Quote: ... Incubation with the primary antibody was carried out at 4°C overnight in a PBS (1x) solution containing anti-Ki67 (Abcam, MA, 1:100), BSA (1%) ...
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bioRxiv - Bioengineering 2024Quote: ... Secondary staining was performed for 2 hours at RT in a PBS (1x) solution containing secondary antibody-TRIC labeled (Abcam, MA, 1:1000), BSA (1% ...
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bioRxiv - Neuroscience 2023Quote: ... Sections were incubated at 4°C for 72 hours in blocking solution (5% bovine serum albumin, 0.2% Triton-X) containing primary antibodies CD45 antibody (Abcam, ab10558, 1:400 dilution), CD11b (Abcam ...
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bioRxiv - Cell Biology 2023Quote: ... Sections were washed with PBS and incubated in the blocking solution containing goat anti-mouse IgM Alexa Fluor 488 (Abcam, ab150121, 1:500) antibody and goat anti-mouse IgG H&L Alexa Fluor 594 (Abcam ...
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bioRxiv - Neuroscience 2022Quote: ... Sections were incubated at room temperature for ~3 hours in secondary antibody solution containing donkey anti-rabbit IgG H&L Texas Red (abcam, Cambridge, UK; ab7081) and donkey anti-goat IgG H&L Alexa Fluor 488 (abcam ...
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bioRxiv - Bioengineering 2022Quote: ... Each well was blocked with 5% BSA-tween solution and incubated with a primary mouse anti-human α-SMA antibody (ab7817, Abcam, Cambridge, UK) solution at a 1:100 dilution overnight at 4°C ...
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bioRxiv - Biochemistry 2023Quote: ... After washing with PBS a 2.5% BSA solution was applied for 1 hour and then incubated with primary anti-human XOR rabbit antibody (1:2000, Abcam 133268 RRID: AB_11154903) and left over-night at 4 °C ...
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bioRxiv - Neuroscience 2022Quote: ... the sections were washed three times in PBST and incubated with secondary antibodies diluted in blocking solution for two hours at room temperature (RT): Goat anti-Rabbit conjugated Alexa Fluor 568 (Abcam #175471, 1:500) and 1:500 Goat anti-Chicken Alexa Fluor 488 (A11039 ...
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bioRxiv - Bioengineering 2024Quote: ... 3D cultured cells were washed with the washing solution and then incubated with goat anti-rabbit secondary antibody Alexa Fluor 488 (ab150077; Abcam, Toronto, ON, Canada) for 2 hours at room temperature ...
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bioRxiv - Immunology 2024Quote: ... Sections were blocked in 10% chicken sera in 5% bovine serum albumin (BSA)/ phosphate buffered saline solution (PBS) for 30 minutes and stained with a rabbit anti-mouse β-actin antibody (ab8227, ABCAM, Cambridge, UK) at 1µg/ml in 1%BSA/PBS ...
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bioRxiv - Neuroscience 2024Quote: ... at room temperature for 1 h and then incubated under agitation at 4° C overnight in a blocking solution containing the following primary antibodies: Rat anti-BrdU/CldU (Abcam, 1:1000, ab6326), and Mouse anti-IdU monoclonal antibody (Abcam ...
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bioRxiv - Neuroscience 2024Quote: ... blocked in TSA-blocking solution for 30 mins and incubated with anti-rabbit-FMRP primary antibody (1: 100, Abcam, Cat# ab17722, Lot# 632949982) at 4C for two consecutive nights ...
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bioRxiv - Immunology 2024Quote: ... in 1X Tris-buffered saline with 0.1% Tween® 20 Detergent (TBS-T) solution followed by incubation in anti-CD63 (anti-CD63 antibody [EPR5702], Abcam, Cambridge, MA, USA), anti-Hsp70 [clone 1B5] (heat shock protein70 ...
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bioRxiv - Biochemistry 2024Quote: ... overnight at 4°C and monoclonal mouse anti-human MUC5AC (Lidell et al., 2008)(1:200) in block solution over night at 4°C (Cat# ab3649, Abcam, Cambridge, UK, RRID:AB_2146844). Secondary antibody was polyclonal donkey anti-rabbit Alexa Fluor 488 (Cat# A-21206 ...
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bioRxiv - Microbiology 2023Quote: ... Cell lines were validated by phenotypic screening and confirmed to be mycoplasma-free using a PCR detection kit (Abcam). All cells were maintained at 37 °C in a 95% humidity and 5% CO2 atmosphere.
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bioRxiv - Physiology 2021Quote: ... Pre-synaptic motoneuron terminals were then labeled by incubating bundles overnight at 4°C in the same blocking solution to which had been added a mouse anti-synaptophysin antibody (AB8049 Abcam, USA; 1:25 dilution). The following morning bundles were washed (5 x 30 min ...
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bioRxiv - Bioengineering 2022Quote: ... The following primary antibodies were prepared in 1% BSA in PBS solutions at the following dilutions: rabbit anti-megalin (Abcam #ab76969, 1:500 dilution), mouse anti-aquaporin-1 (Santa Cruz #sc-32737 ...
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bioRxiv - Neuroscience 2021Quote: ... for 1 h at room temperature and then with primary antibodies diluted in the blocking solution overnight (anti-PV primary antibody, ab11427, Abcam, Cambridge, UK; 1:1000) at 4°C ...
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bioRxiv - Cancer Biology 2023Quote: ... The lower part of the membrane was blocked with 3% BSA in phosphate-buffered saline (PBS) solution followed by incubation with rabbit polyclonal anti-GFP (Abcam, UK; #ab290, 1:1,000) primary antibody at RT for 1 h for eGFP detection (30 kDa) ...
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bioRxiv - Neuroscience 2023Quote: ... Following primary antibody incubation brains were washed 4 times for 15 minutes each in a 3% salt solution (in 1X PBST) and transferred to secondary antibodies (Abcam: ab175673; ThermoFisher: A-21236) in 1XPBST and incubated overnight at 4°C on a nutator ...
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bioRxiv - Neuroscience 2023Quote: ... and the washed pellet was resuspended in 250 ul of the following antibody solution: recombinant Alexa Fluor® 647 anti-ERG (1:100 dilution; ab196149, Abcam, Massachusetts, United States) in 0.5% BSA + 0.1% Triton in PBS ...
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bioRxiv - Cancer Biology 2023Quote: ... cells were stained overnight at 4°C in blocking solution with the following antibodies and dilutions: Alexa Fluor® 488 anti-S100 beta (1:1000, Abcam, #ab196442, clone EP1576Y), PE anti-Nestin (1:150 ...
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bioRxiv - Bioengineering 2023Quote: ... Cells were then incubated overnight in primary antibody in the blocking solution at 4°C using 1 or more of the following antibodies: α-SMA antibody (ab7817, 1:500, Abcam, Cambridge, MA, USA), FAP antibody (ab53066 ...
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bioRxiv - Cell Biology 2024Quote: ... Primary antibodies were diluted in 1% normal goat serum (NGS) blocking solution as follows: anti-rhodopsin 1D4 for mouse rod opsin (1:200; Abcam, St. Louis, MO, USA), R/G cone opsins (1:100 ...
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bioRxiv - Neuroscience 2022Quote: ... collected and subjected to real-time PCR in a similar way as ChIP with following antibodies: mouse anti-5mC (Abcam) and rabbit anti-5hmC (Active Motif) ...
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bioRxiv - Plant Biology 2022Quote: ... the mCherry coding sequence and the AAR2 genomic fragment were PCR-amplified and recombined into the pCambia1300 vector (abcam, ab275754). For 35S::AAR2-nYFP ...
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bioRxiv - Molecular Biology 2024Quote: Mycoplasma PCR test was performed on culture supernatants (at least 48h without medium change and confluence at least 80%) using Kit Mycoplasma PCR Detection Kit (Abcam) according to the manufacturer’s instructions.
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bioRxiv - Immunology 2022Quote: ... table 1) were diluted in blocking solution (F4/80 [Bio-Rad]: 10 µg/ml, Ly6G [abcam]: 1 µg/ml, MHC-II [BioLegend]: 5 µg/ml), CD163 [Invitrogen] ...
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bioRxiv - Plant Biology 2023Quote: ... and then was first incubated with rabbit anti-AKIN10 antibody (1:500 dilution) followed by incubation with Veriblot IP detection reagent (Abcam, 1:500 in blocking solution). Protein bands were detected with SupersignalTM west pico chemiluminescent substrate (Thermofisher ...
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bioRxiv - Molecular Biology 2024Quote: ... 1:500 rabbit anti-PARP1 Abcam ab227244; 1:500 rabbit anti-PCNA Abcam ab18197, 1:500 mouse anti-PCNA Abcam ab29 diluted in blocking solution). After antibody incubation ...
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bioRxiv - Neuroscience 2022Quote: ... the slices were incubated in the solution of primary antibodies: anti-glial fibrillar acidic protein (GFAP) rabbit polyclonal antibody (Abcam, Cambridge, UK, catalog number ab7260) and anti-neuronal nuclear protein (NeuN ...
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bioRxiv - Neuroscience 2024Quote: ... United States).The following day slices were washed 4 × 10 min followed by 2 h incubation in secondary antibody solution containing goat-anti-rabbit Alexa Fluor 488 (1:500; Abcam, ab150077, Waltham, MA, United States), goat-anti-chicken Alexa Fluor 488 (1:500 ...
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bioRxiv - Molecular Biology 2022Quote: ... The coding sequence of p21 was PCR amplified from flag-p21-WT(55) (a gift from Mien-Chie Hung, Abcam #16240) and cloned into pRetro-X-tight-PUR (Clontech) ...
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bioRxiv - Neuroscience 2020Quote: ... slices were blocked for two hours at room temperature and incubated in primary antibody in carrier solution (goat anti-calretinin, 1:1000, Swant; rabbit anti-NeuN, 1:3000, Abcam; rabbit anti-GABA, 1:500, SigmaAldrich) overnight at 4 °C ...
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bioRxiv - Neuroscience 2023Quote: ... Brain sections were incubated overnight at 4°C with the following primary antibodies diluted in blocking solution: chicken polyclonal anti-mouse GFAP (Abcam ref. ab4674, RRID AB_304558, 1:3000), rabbit polyclonal anti-mouse IBA1 (Wako ref ...
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bioRxiv - Bioengineering 2024Quote: ... sections were washed three times with PBS for 15 min and incubated in blocking solution (PBS with 10% [v/v], goat serum [Abcam #7481] and 0.5% Triton X-100) for 2 hr at room temperature ...
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bioRxiv - Genomics 2022Quote: ... Cell growth was monitored via microscopy during 25 days to select those wells which were observed to contain single colonies and USP47 knockout was confirmed in those monoclonal HAP1 cell lines first via PCR and then reconfirmed by western blot using the USP47 rabbit polyclonal antibody (Abcam, Cat# ab97835).
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bioRxiv - Cell Biology 2021Quote: ... Non-specific interactions were blocked for 1 hour with blocking solution (3% BSA, Sigma ref. A9647, 2% donkey serum, Abcam ref. ab7475, 0,3% Triton X-100, PBS). Primary antibodies were diluted in the blocking solution and incubated overnight at 4°C ...