Labshake search
Citations for abcam :
451 - 500 of 638 citations for 5M Betaine Solution PCR Grade since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cell Biology 2023Quote: ... The following primary antibodies were incubated with the blots overnight at 4 °C in blocking solution: anti-myc-tag (1:5,000, rabbit pAb, ab9106, Abcam, UK), anti-Dclk1 (1:1,000 ...
-
bioRxiv - Neuroscience 2022Quote: ... The sections were washed three times with PBST and incubated with primary antibodies diluted in blocking solution: 1:500 Rabbit anti-Iba1 (ab178846, Abcam) and 1:250 chicken anti-GFP (A10262 ...
-
bioRxiv - Neuroscience 2023Quote: ... PFC sections were incubated overnight at 4°C in a blocking solution containing primary antibodies against green fluorescent protein (GFP; 1:1000 dilution, raised in goat, AB6673, Abcam). VTA sections were incubated overnight in a blocking solution containing antibodies for green fluorescent protein (GFP ...
-
bioRxiv - Neuroscience 2023Quote: ... VTA sections were incubated overnight in a blocking solution containing antibodies for green fluorescent protein (GFP; 1:1000 dilution, raised in goat, AB6673, Abcam) and tyrosine hydroxylase (TH ...
-
bioRxiv - Neuroscience 2023Quote: ... brain sections were incubated for 24 h at 4°C in the same pre-incubation stock solution (PB+Triton+Serum) containing different combinations of the following primary antibodies: ab6326 (Abcam) for CldU ...
-
bioRxiv - Cell Biology 2023Quote: ... Cells were washed and incubated for 1 H in a 1:200 primary solution of Anti-pRAB7A antibody (ab302494, Abcam).
-
bioRxiv - Developmental Biology 2023Quote: ... The samples were incubated overnight at 4°C in a staining solution containing rabbit anti-SOX10 (1:100, Abcam, ab227680).The next day ...
-
bioRxiv - Genomics 2023Quote: ... Tissues were stained at 4 °C overnight using antibodies diluted in blocking solution: anti-EpCAM-AF594 (abcam, ab286811; 1:200) and anti-Vimentin-AF488 (BioLegend ...
-
Mitigating a TDP-43 proteinopathy by targeting ataxin-2 using RNA-targeting CRISPR effector proteinsbioRxiv - Bioengineering 2023Quote: Tissue sections were washed three times with PBS and incubated in blocking solution [PBS with 10% (v/v) donkey serum (Abcam) and 1% Triton X-100] for 2 hr at RT ...
-
bioRxiv - Genomics 2023Quote: ... Beads were then resuspended in 1 mL BSA solution and 5 μL of α-5-hmU antibody (Abcam Cat # ab19735) were added ...
-
bioRxiv - Cell Biology 2023Quote: ... slides were stained with DAPI 1:1000 solution in PBS and mounted in anti-fade fluorescence mounting medium (Abcam, ab104135). They were kept at 4°C prior to image acquisition using Cell DIVE Multiplex Slide Scanner (Leica Microsystems) ...
-
bioRxiv - Bioengineering 2023Quote: ... sections were washed three times in PBS for 15 min and incubated in blocking solution [PBS with 10% (v/v) donkey serum (Abcam) and 0.5% Triton X-100] for 2 hr at RT ...
-
bioRxiv - Neuroscience 2024Quote: ... Slices were incubated overnight at 4 °C in the blocking solution with primary antibody (Iba1: rabbit anti-Iba1, ab178846 Abcam, 1:200 dilution; GFAP: Goat anti-GFAP, ab53554 Abcam, 1:200 dilution ...
-
bioRxiv - Neuroscience 2024Quote: ... cultures were treated with 7-Chlorokynurenic acid (7-CKA) (final concentration 50 μM; 1 μL stock solution in DMSO in 1 mL media; ab120024; Abcam) and D-AP5 (final concentration 100 µM ...
-
bioRxiv - Neuroscience 2024Quote: ... 60 um coronal sections were cut and incubated overnight in a primary antibody solution with polyclonal chicken anti-GFP (ab13970, Abcam). The following day ...
-
bioRxiv - Cell Biology 2024Quote: ... Cells were then incubated for 30 minutes at room temperature or overnight at 4°C with the indicated primary antibodies diluted 1:100-1:200 in blocking solution (mouse IgG1 anti-MIC60 [110329; Abcam] ...
-
bioRxiv - Bioengineering 2024Quote: ... Primary antibody solution for Aldh1l1 and vimentin staining: 2 mL blocking buffer containing 20 μL anti-Aldh1l1 antibody (Abcam, AB56777) and 20 μL anti-vimentin (Abcam ...
-
bioRxiv - Bioengineering 2024Quote: ... the membrane was incubated overnight at 4°C or 1 hour at room temperature with primary antibody solution (CD81, Santa Cruz Biotechnology, sc-166029, 1:200; TSG101, abcam, ab125011, 1:1,000; Alix, abcam, ab117600 ...
-
bioRxiv - Molecular Biology 2024Quote: ... were blocked with 0.5% BSA (w/v) in phosphate-buffered saline (PBS) solution and conjugated with anti-H3K9me3 (Abcam, ab8898) antibody ...
-
bioRxiv - Neuroscience 2024Quote: ... Slices were incubated overnight at 4 °C in the blocking solution with primary antibody (Iba1: rabbit anti-Iba1, ab178846 Abcam, 1:200 dilution ...
-
bioRxiv - Microbiology 2024Quote: ... Serine racemase treatment of human serum was performed with the addition of 5 µl of proprietary serine racemase solution from a DL-serine assay kit (Abcam) to 1 ml of serum for 3 h ...
-
bioRxiv - Neuroscience 2024Quote: ... These sections were incubated overnight at 4⁰C in blocking solution containing chicken anti-GFP antibody (1:1000, Abcam ab13970). The next day ...
-
bioRxiv - Neuroscience 2024Quote: ... cells were washed in PBS then incubated for 30 min at 37°C in Neurobasal phenol-free working solution supplemented with TMRM 20 nM (Abcam), Calcein 4 µM (Insight Biotechnology) ...
-
bioRxiv - Cell Biology 2024Quote: ... Primary antibody solutions were prepared in TBS++ (TBS, 0.1% Triton X-100 and 3% donkey serum) against MAP2 1:2000 (Abcam; ab5392), LMX1A (Abcam ...
-
bioRxiv - Neuroscience 2024Quote: ... The samples were then incubated in secondary antibody solution (polyclonal biotinylated goat anti-rabbit antibody, Abcam, cat. Ab6720, 1:400) for 30min at room temperature ...
-
bioRxiv - Cancer Biology 2024Quote: ... endogenous peroxidase inactivation with 3% H2O2 was performed on dewaxed specimen followed by blocking with avidin biotin blocking solution (ab64212, Abcam) and 5% BSA in TBST ...
-
bioRxiv - Physiology 2024Quote: ... 0.2% fish gelatin in 1 x PBS) BD Biosciences) and goat anti-rabbit 4-HNE (1:200 in blocking solution; Abcam) antibodies ...
-
bioRxiv - Cell Biology 2024Quote: ... 0.3% triton and 1%BSA) and incubated in blocking solution with first antibody (1 hour, room temperature): chicken anti-GFP (1/1800, ab13970, Abcam); rabbit anti-Lamin B1 (1/300 ...
-
bioRxiv - Bioengineering 2024Quote: ... solution for 1 hour at room temperature cells were immunostained with rabbit anti-β-III-tubulin (Abcam ab18207, 1:1000) primary antibody ...
-
bioRxiv - Biophysics 2024Quote: ... bovine serum albumin (BSA) was removed (when applicable) from the stock antibody solution with an antibody conjugation kit according to manufacturer instructions (Abcam). After BSA removal ...
-
bioRxiv - Neuroscience 2021Quote: ... Coverslips were placed on a 45 µL drop of primary antibody solution consisting of 1% BSA in PBS with primary antibodies as follows: GLUT4 (Abcam, 33780) 1:500 ...
-
bioRxiv - Immunology 2021Quote: ... Sections were then washed in TBS and stained in 10% FBS in TBS-T (0.05% Tween-20) blocking solution with primary antibodies: anti-GFP 1:2000 (Abcam Cat# ab13970), anti-RFP 1:1000 (Rockland p/n ...
-
bioRxiv - Genetics 2021Quote: ... were incubated in blocking solution (0.5% bovine serum albumin in PBS) with 3 μg of antibody to histone-H3 K27ac (Abcam, cat. # ab4729) for 3 h at 4°C ...
-
bioRxiv - Developmental Biology 2020Quote: ... Slides were incubated in primary antibodies diluted in blocking solution overnight at 4°C as follows: rabbit anti-CASP3 (1:500, Abcam, ab49822); mouse anti-COL2A1 (1:400 ...
-
bioRxiv - Neuroscience 2021Quote: ... injected with vehicle (VEH = 0.9% aqueous solution of NaCl and 5% ethanol) or MTZ (10 mg/kg, ab120068, Abcam, Cambridge, UK). The 10 mg/kg daily dosage in mice is equivalent to 50 mg/day in humans ...
-
bioRxiv - Neuroscience 2021Quote: ... Microglia were probed with a 24h incubation at 4°C with either a rabbit antihuman IBA1 antibody diluted in blocking solution (1:200, 019-19741, Wako chemicals, USA) or a goat anti-human IBA1 antibody (1:200, ab5076, Abcam, UK). We used additional microglial markers including mouse anti-MHC-II/HLA-DP/DQ/DR (1:250 ...
-
bioRxiv - Bioengineering 2022Quote: ... A solution of 0.05% of Tween-20 was used to wash the samples and a solution of goat anti-Mouse secondary antibody IgG was added (1:1000, Abcam # ab6785) and incubated for 2 h at room temperature ...
-
bioRxiv - Genetics 2020Quote: ... and then incubated in blocking solution overnight at 4°C with the following antibodies: rabbit monoclonal anti-ATP6V1A (1:1000)(Abcam EPR19270), rabbit polyclonal anti-GNE (1:1000)(Proteintech 25079-1-AP) ...
-
bioRxiv - Immunology 2021Quote: ... The membrane was then washed 3×15 minutes in PBST and then incubated in secondary antibody solution (PBST + 5% BSA + Goat a-Rabbit-HRP 1:5000) (Abcam, ab205718) for one hour at room temperature ...
-
bioRxiv - Bioengineering 2020Quote: ... In the next step the cells were washed with 3% BSA solution and incubated with 1x Phalloidin-iFluor594 (Abcam, stock 1000x) solution (in 1% BSA ...
-
bioRxiv - Neuroscience 2021Quote: ... for 30 min at room temperature and then incubated in blocking solution containing goat anti-GFP (to recognize BFP, 1:1000, Abcam, ab6673) and rabbit anti-RFP (to recognize mCherry ...
-
bioRxiv - Neuroscience 2020Quote: ... Tissue was then incubated for 2 hours at room temperature in a PBTGS secondary antibody solution including goat anti-mouse Alexa568 (1:200; Abcam [ab175473]), goat anti-rabbit Cy2 (1:200 ...
-
bioRxiv - Bioengineering 2022Quote: ... the actin cytoskeleton was stained with Alexa Fluor 488 Phalloidin in PBS (containing 1% DMSO from the original stock solution, Abcam®) for 40 min at room temperature in a dark chamber ...
-
bioRxiv - Microbiology 2021Quote: ... Plates were then washed five times with PBS-T and 100 μL of TMB ELISA Substrate Solution (Abcam, Cambridge, MA, USA) was added to all wells of the plate and incubated for 5 minutes before the reaction was stopped ...
-
bioRxiv - Neuroscience 2022Quote: ... slices were incubated in a primary antibody blocking solution including both rabbit anti-PV (1:500; Abcam Cat. #ab11427, Pittsburgh, PA), and WFA lectin fluorescein (1:100 ...
-
bioRxiv - Developmental Biology 2022Quote: ... 0.7% Triton X-100 in 1X PBS) for 2 hours at room temperature and incubated in blocking solution with anti-GFP antibody (1:500, Abcam, ab6658) overnight at 4 ºC ...
-
bioRxiv - Neuroscience 2020Quote: ... neuronal cultures were fixed by incubating cells with a solution of 4% PFA and 4% sucrose for 10 minutes at room temperature before incubation with primary antibody (Abcam, ab6556). In another set of experiments ...
-
bioRxiv - Neuroscience 2020Quote: ... BLA slices were incubated overnight at 4°C in a solution containing 0.1% Triton X and 3% normal goat serum in PBS and chicken anti-GFP (1:1000, Abcam, ab13970, polyclonal). They were then rinsed in PBS (3 × 10min ...
-
bioRxiv - Bioengineering 2020Quote: ... Membranes were blocked for 1 hr at RT in 3% BSA PBS 0.1% Tween-20 (PBS-T) and then incubated overnight at 4°C with primary antibody (rabbit anti-RGS9, 1:500 in blocking solution; Abcam, UK). After washing three times in PBS-T ...
-
bioRxiv - Immunology 2022Quote: ... and pelleted again before resuspension in HMI-9 or staining solution containing an anti-fentanyl monoclonal antibody (provided by M. Pravetoni, University of Minnesota) conjugated to FITC (Abcam, ab102884) for analyzing sortagging success ...