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Citations for abcam :
3801 - 3850 of 3865 citations for Human Hypoxia inducible factor 3 alpha HIF3A ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: ... then were fixed with 4% paraformaldehyde (PFA) for 15 min at room temperature and stained using the Senescence-Associated β-Galactosidase Staining Kit (Abcam ab65351), following the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2020Quote: The glutamate concentration in the conditioned medium of primary microglial cultures was assayed with the glutamate colorimetric assay kit from Abcam (no. 83389), according to the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2020Quote: Quantification of FabI via a C-terminal GFP tags was performed using a GFP quantification kit from AbCam (Cambridge, UK, Cat # ab171581) according to manufacturer’s instructions.
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bioRxiv - Neuroscience 2020Quote: Indirect assessment of glutamate uptake by resting iPSC-astrocyte lines was done using a colorimetric glutamate assay kit following the manufacturer’s recommendations (Abcam; Cat. no.: ab83389). Briefly ...
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bioRxiv - Cancer Biology 2019Quote: ... Protein lysates from each subcellular fraction (cytoplasm, mitochondria, and nucleus) were obtained from PNT1 and LNCaP cells using the cell fractionation kit (Abcam, VIC, Australia) according to the manufacturer’s protocol.
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bioRxiv - Microbiology 2022Quote: Enzyme activity in vaginal swab supernatants and cell pellets was quantified using the α-Glucosidase Activity Assay Kit (Abcam, Waltham, MA, USA), Enzchek Ultra Amylase Kit (Invitrogen ...
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bioRxiv - Neuroscience 2021Quote: ... Conjugation to FITC or ammonium peroxidase was carried out using Abcam’s alkaline phosphatase and FITC conjugation kits (Abcam ab102850 and ab102884 respectively) according to the protocols outlined in the manufacturer’s instruction manuals ...
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Adipocyte vesicles: ‘all-in-one’ packages that stimulate tumor mitochondrial metabolism and dynamicsbioRxiv - Cancer Biology 2019Quote: ... The organic phase was evaporated under liquid nitrogen and lipids were resuspended in 50µL of fatty acid assay buffer (Free Fatty Acid Assay Kit, Abcam, Cambridge, MA, USA). FA (contained in 5µL of buffer ...
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bioRxiv - Synthetic Biology 2020Quote: ... The concentrations of NAD(H) (total NAD+/NADH) and NADH only were measured by the NAD+/NADH assay kit (Abcam, Cambridge, UK) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2019Quote: ... the cells were stained using the EXPOSE Mouse and Rabbit Specific HRP/DAB Detection IHC kit (Abcam, United States; Cat. no. ab80436). The enzyme expression was visualized with the mouse monoclonal antibody anti-SOD2 (Santa Cruz ...
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bioRxiv - Immunology 2020Quote: ... Followed by capture of SARS-CoV-2 receptor binding domain which was biotinylated using the lightning-link type-A biotinylation kit(Expedeon/Abcam, 370-0005) for 180s at 10ul/min ...
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bioRxiv - Neuroscience 2020Quote: Absolute quantification of sialic acid in different glycoconjugate fractions was performed using the Sialic Acid (NANA) Assay Kit (Ab83375, Abcam, Cambridge, MA) according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2022Quote: Cell cycle analysis was performed by propidium iodide (PI) staining following the protocols from the propidium iodide flow cytometry kit (Abcam, Cat. ab139418). Briefly ...
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bioRxiv - Cell Biology 2024Quote: ... None of the cultures or treatments showed signs of cell cytotoxicity using the using the LDH-Cytotoxicity Assay Kit II (Abcam, cat. #ab65393) to analyze 50 ul fresh culture media (not shown) ...
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bioRxiv - Neuroscience 2024Quote: ... 30mg of protein from scraped cells at day N1 and N14 were analyzed with the Cathepsin D Activity assay kit (ab65302; Abcam, Waltham, MA) following the manufacturer’s instructions.
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bioRxiv - Physiology 2024Quote: Mitochondrial membrane potential was measured in γY4KD cells with Pparγ silenced with siRNA and their scrambled control (Scrl) using Mitochondrial Membrane Potential Assay kit (Abcam, Cat# ab113852). Cells were plated on collagen coated 24-well plate at density of 50,000 cells/well (5 well/per group) ...
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bioRxiv - Neuroscience 2024Quote: Choline and acetylcholine were quantified in rat cortical tissue from all experimental groups using a choline/acetylcholine assay kit (ab65345, Abcam, Cambrige, UK). 10 mg of cortical fresh tissue were harvested ...
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bioRxiv - Bioengineering 2024Quote: ... 100 ug of anti-SARS-CoV-2/S2 (1A9) antibody was conjugated to A647 using the Lightning-Link Conjugation Kit (Abcam, Cat# ab269823) according to the manufacturer’s protocol.
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bioRxiv - Plant Biology 2023Quote: ... Two microliter aliquots of the supernatants were transferred to wells of a 96-well plate prefilled with 98μl of assay buffer of the ammonia assay kit from AbCam (AbCam Cat # ab102509), and ammonia levels were quantified according to the manufacturer’s guidelines ...
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bioRxiv - Genomics 2023Quote: The cholesterol assay was carried out according to the manufacturer protocol using the Cholesterol/Cholesterol Ester Assay Kit (ab65359, Abcam, Cambridge, UK) and normalized to total protein content as determined by Bradford Assay.
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bioRxiv - Biochemistry 2023Quote: Right Ventricular Mitochondrial Enrichments: RV mitochondrial enrichments were isolated from the same PAB and control piglets with a mitochondrial isolation kit (Abcam, Cambridge, MA) as previously described(10 ...
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bioRxiv - Bioengineering 2023Quote: ... was dissected from the constructs at the end of maturation time (day 15) and tested via a colorimetric CK Activity Assay Kit (Ab155901, Abcam, Cambridge, UK) according to the manufacturer’s instructions ...
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bioRxiv - Pathology 2023Quote: ... regularly spaced (500 µm) along the rostrocaudal axis were stained with a Prussian blue and nuclear fast red stain kit (Abcam, Cambridge, UK) following the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2023Quote: RCC243 cells were treated with 5 μM MS023 for the indicated time periods and then cells were trypsinized and stained using the Propidium Iodide Flow Cytometry Kit (Abcam, #ab139418, Abcam) based on the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... end-stage bulk tumor and 4- to 15-weeks control HDTVi liver protein lysates (50 μg) were analyzed using Fluorometric Caspase-1 Assay Kit (Abcam, cat.no. ab273268) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: D2HG levels were measured in adherent cultures of 1 x 107 chondrosarcoma cells treated with increasing concentrations of Enasidenib for 48 hours using the D2HG colorimetric Assay Kit (Abcam, Cambridge, UK) following the manufactureŕs recommendations.
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bioRxiv - Molecular Biology 2023Quote: A pair of parotid salivary glands were harvested from each mouse (n=5/group) and directly placed in 500 uL of Lactate Assay Buffer from the L-Lactate Assay Kit (ab65331, Abcam, Cambridge, UK), 10 uL of protease inhibitor (PI ...
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bioRxiv - Cancer Biology 2024Quote: ... the adherent monolayers were incubated with 100 µl/well cell lysis buffer from the cAMP Direct Immunoassays Kit (Fluorometric, ab138880, Abcam, Cambridge, UK), while the gliomaspheres were collected ...
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bioRxiv - Pathology 2024Quote: ... Masson’s trichrome staining was performed to assess endometrial fibrosis in sections within a 20 μm distance of the FOXA2-assayed sections using a kit (ab150686, Abcam; Waltham, MA) according to manufacturer instructions.
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bioRxiv - Neuroscience 2024Quote: ... The activity of cytosolic- or secretory-phospholipase-A2 was measured according to the procedures described in the kit manuals (Abcam, Cambridge, UK).
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bioRxiv - Cell Biology 2024Quote: ... MockIP and WCL were aliquoted into wells in a 96-well plate and assayed using a fluorometric Cathepsin D activity assay kit (Abcam. Cat# Ab65302) that is based on quantifying the hydrolysis of the preferred cathepsin-D substrate sequence GKPILFFRLK(Dnp)-D-R-NH2 ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... the sections were incubated with histofine simple stain kit Rat MAX-PO(G) (414331F, Nichirei, Tokyo, Japan) or goat anti-chicken IgY conjugated biotin (ab6876, abcam plc, Cambridge, UK) for 30 minutrs at RT ...
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bioRxiv - Immunology 2021Quote: ... Free fatty acid concentration in plasma (diluted 1:25) of clamped mice was determined using a quantitative Free Fatty Acid Assay Kit (Abcam, Cambridge, Great Britain). Absorbance was measured with the Filter Max F5 device (Molecular Devices ...
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bioRxiv - Cell Biology 2019Quote: ... we used the Maxpar Antibody Labeling Kit (Fluidigm) according to manufacturer’s instructions for addition of the respective metal tags: Axin2 (145-Nd, Abcam, ab32197, 2 µg/ml), p-MEK1/2 [S217/221] (151-Eu ...
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bioRxiv - Neuroscience 2020Quote: ... sections were preincubated (1-hour) at RT in a protein blocking solution (Expose Rabbit-Specific HRP/DAB detection IHC Kit, Abcam, Cambridge, UK, ab80437). Then ...
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bioRxiv - Microbiology 2021Quote: ... felis C4 butanol extract were measured with 2’,7’-dichlorofluorescein diacetate (DCFDA) following the manufacturer’s instructions (DCFDA/H2DCFDA Abcam cellular ROS assay kit). Briefly ...
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bioRxiv - Molecular Biology 2022Quote: ... The cells were rinsed with phosphate-buffered saline (PBS) and then stained in the culture well using the Adipogenesis Assay Kit (cell-based, Abcam Catalog No. ab133102). The dye was eluted using the kit’s elution buffer and quantified using a microplate reader at 490 nm wavelength ...
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bioRxiv - Immunology 2022Quote: ... media was collected and LDH activity measurement was carried out in 96-well plate (Costar, cat no. 3370) according to LDH assay kit (Abcam, cat. no ab102526). Absorbance measurement at 450 and 570 nm was carried out using microplate reader (BioTek ...
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bioRxiv - Molecular Biology 2021Quote: ... The serum is at the top layer and was collected for subsequent colorimetric analysis of the total cholesterol using the Cholesterol/ Cholesteryl Ester Assay Kit (Abcam, Cambridge, MA, USA) following manufactures protocol.
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bioRxiv - Physiology 2020Quote: ... Nuclear sirtuin 1 content and activity was assessed using 10 µg (5 μL) of isolated nuclear protein fractions through a fluorometric assay (Sirt1 Activity Assay Kit, #ab156065, Abcam, Whitby, Ontario, Canada). Another set of samples weighing 5-10 mg were homogenized in RIPA buffer (150 mM sodium chloride ...
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bioRxiv - Microbiology 2021Quote: ... while 100 μg of anti-SARS-CoV-2 S were conjugated with Alexa Fluor® 488 Lightning-Link® Conjugation Kit (Abcam # ab236553). Each experiment was repeated with similar results using anti-SARS-CoV-2 N rabbit polyclonal Ab (GeneTex # GTX135357 ...
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bioRxiv - Neuroscience 2023Quote: ... Triton X-100 in 1X PBS for 20 min and then endogenous biotin was blocked using Avidin/Biotin blocking kit (Abcam; cat. no. ab64212) to reduce non-specific background ...
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bioRxiv - Cell Biology 2024Quote: The amount of collagen in liver tissues was determined by measuring the content of hydroxyproline using a Hydroxyproline assay kit (Abcam, Cat#MAK008-1KT) as described by the manufacturer ...
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bioRxiv - Cell Biology 2023Quote: ... and the quantification of intracellular and extracellular total cholesterol and its fractions was measured using the Cholesterol Assay Kit (Abcam, Cambridge, United Kingdom) according to the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2023Quote: LB samples that were autoclaved or not autoclaved were subjected to quantification of NADH/NAD+ using an NAD+/NADH Colorimetric Assay Kit (ab65348, Abcam, Cambridge, United Kingdom). Autoclaved samples were measured before and after either chronoamperometry or cycle voltammetry treatment ...
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bioRxiv - Cell Biology 2023Quote: The chymotrypsin-like activity of the 20S proteasome was assessed using a commercially available fluorometric proteasome activity assay kit (Abcam, catalog number: ab107921) according to the manufacturer’s guidelines ...
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bioRxiv - Cell Biology 2022Quote: Chromatin accessibility in isolated cardiac fibroblasts or whole heart from C57BL/6J mice was measured using the Chromatin Accessibility Assay Kit (Abcam, cat. no. ab185901) according to the manufacturer’s instructions ...
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bioRxiv - Physiology 2023Quote: ... Unless specified below, all reagents were from MilliporeSigma (St. Louis, MO, USA). LDH assay kit (catalog no. ab65393) was from Abcam (Cambridge, MA, USA). P ...
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bioRxiv - Molecular Biology 2023Quote: Three 5 µm sections taken at 150 µm intervals were used for analysis from each animal and were stained with hematoxylin and eosin using the reagents provided in Oil Red O Stain Kit (Abcam, Waltham, MA, USA) for hematoxylin and eosin purchased from StatLab (StatLab ...
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bioRxiv - Cancer Biology 2024Quote: ... Paraffin-embedded slides were also used for the TUNEL assay and processed according to manufacturer instructions (Abcam, TUNEL Assay Kit - HRP-DAB ab206386). The prepared sections were analyzed using a Nikon Eclipse 80i microscope ...