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Citations for abcam :
3551 - 3600 of 3865 citations for Human Hypoxia inducible factor 3 alpha HIF3A ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2022Quote: ... The glycogen content of the homogenates was measured using a Glycogen Assay Kit II (ab169558, Abcam, Cambridge, United Kingdom) according to the manufacturer’s instructions.
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bioRxiv - Physiology 2022Quote: Skeletal muscle GSH and GSSG was measured using the fluorometric GSH/GSSG Ratio Detection Assay Kit II (Abcam 205811). Briefly ...
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bioRxiv - Cell Biology 2020Quote: Norepinephrine (NE)-induced-glucose uptake was assayed according to the established protocol from a commercial glucose uptake kit (Abcam). In brief ...
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bioRxiv - Cell Biology 2020Quote: ... 48h and 72h with 1 µM of (-)-ILV or 0.1% DMSO and apoptotic cells were analyzed by using Annexin V FITC apoptosis detection kit (Abcam). Following treatment ...
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bioRxiv - Molecular Biology 2020Quote: ... Cell pellets were lysed and mitochondrion was purified according to the manufacturer’s protocol in a mitochondrial isolation kit for cultured cells (Abcam). The activity was determined by following the manufacturer’s protocol with a standard curve.
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bioRxiv - Bioengineering 2019Quote: ... Plates were washed with washing buffer (PBS 0.1% Triton) and 200 µL/well Horseradish peroxidase conjugated-Infliximab (HRP conjugation Kit, abcam) (0.2 µg/mL IFX-HRP ...
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bioRxiv - Cancer Biology 2021Quote: NADPH in lysates of cell lines or A549 xenografts was determined using a colorimetric NADPH Assay Kit (Abcam’s, ab186031), following the manufacturer’s instructions on LC cells and A549 xenografts treated with either FASNi or vehicle for 4 days ...
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bioRxiv - Biochemistry 2020Quote: ... Tissues were lysed by 5% Total glutathione content was measured using an Intracellular glutathione (GSH) Detection Assay Kit (Abcam) as described in the manufacturer’s protocol ...
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bioRxiv - Bioengineering 2021Quote: ... the inner lumen of the trachea was labeled fluorescently using carboxyfluorescein succinimidyl ester (CFSE) cell labeling kit (ab113853, Abcam). The tissue labeling was performed according to the company’s instruction prior to the de-epithelialization of the trachea ...
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bioRxiv - Cell Biology 2019Quote: Cytosolic and nuclear proteins were extracted from cultured NRVMs using the Nuclear Extraction Kit (Catalog. #: NC113474; Abcam, Cambridge, MA) according to the manual provided by the manufacturer ...
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bioRxiv - Immunology 2021Quote: ... B38 (10 μg) and CC12.1 (100 μg) were coupled with HRP using the ab 102890 – HRP Conjugation kit (Lightning Link, Abcam) following the manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... Protein concentration in endothelial cell lysates were measured using bicinchoninic acid assay (BCA) Protein Assay Kit (Abcam, Cambridge, UK). NOS production was measured indirectly by Griess reagent in samples using Nitric Oxide Synthase (NOS ...
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bioRxiv - Molecular Biology 2022Quote: ROS production was measured in serum-treated primary Sertoli cultures using DCFDA/H2DCFDA-Cellular ROS Assay Kit (ab113851, Abcam) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2022Quote: ... the spheroid growth/viability was measured using Cell Counting Kit-8 (WST-8 / CCK-8; Cat. no. ab228554, Abcam). On day 15 of 3D spheroid culture (after 10 days of dox-induction) ...
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bioRxiv - Pathology 2022Quote: ... Anti-SARS-CoV-2 was conjugated to PE / R-Phycoerythrin (Expedeon Lightning-Link R-PE Conjugation Kit / Abcam, ab102918), while anti-CD31 was linked to Alexa Fluor 488 (Abcam ...
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bioRxiv - Physiology 2022Quote: ... The homogenates were centrifuged at 14’000 g for 2 min and the supernatant was used to measure triglyceride using a triglyceride quantification kit (Abcam) by following the manufacture’s instruction ...
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bioRxiv - Neuroscience 2023Quote: ... amniotic fluid and fetal brain alcohol concentrations were mesurted using the Ethanol Assay Kit (Cat. #ab272531; Abcam, Cambridge, UK).
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Staining was completed using Mayer hematoxylin and 0.2 % eosin (Histolab) or Periodic Acid Schiff (PAS) Stain Kit (Mucin Stain) (Abcam). Imaging of the slides was performed using an Olympus BX60F microscope with an SC50 camera (Olympus ...
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bioRxiv - Developmental Biology 2022Quote: ... Metabolites were extracted and ATP concentration (mM/embryo) measured using a luminescent ATP Detection Assay kit (Abcam, catalog # ab113849), according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2022Quote: ... The amount of free phosphate molecule generated by the reaction was colorimetrically quantified with a Phosphate Assay Kit (Abcam). The amount of released phosphate was determined from a standard curve generated after plotting the absorbance value against increasing known concentrations of free phosphate molecule.
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bioRxiv - Immunology 2024Quote: ... Mitochondria and cytosolic fractions of each group were isolated utilizing the Mitochondria Isolation Kit for cultured cells (#ab110170, Abcam) following the manufacturer’s protocol ...
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bioRxiv - Bioengineering 2023Quote: ... The cytotoxicity of the different types of PBFSU NPs were evaluated using an MTS Assay Kit (Abcam, Cat# ab197010). INS-1 β-cells were cultured in a 96-well plate at 15000 cells/well for 24 h and subsequently treated with the respective doses of the different types of PBFSU NPs for 24 hours ...
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bioRxiv - Microbiology 2024Quote: Total extracts or the supernatant of Leptospira (5.5 µg) was used to determine the collagenase activity using the Collagenase Activity Assay Kit (Abcam), according to the manufacturer’s instructions.
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bioRxiv - Developmental Biology 2024Quote: ... The tissues were then stained by hematoxylin or with periodic acid staining using a PAS Stain Kit (Abcam, ab150680) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2024Quote: ... cell supernatant and adhered cells were collected and stained for flow cytometry using a Propidium Iodide kit (Abcam ab139418) using manufacturer’s protocol ...
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bioRxiv - Pathology 2023Quote: ... OCT-embedded abdominal aorta sections were fixed in 10% formalin and treated according to the manufacturer’s instructions (Elastic Stain Kit, AB150667, Abcam). Images were captured on a Nikon ECLIPSE 80i microscope with the aid of NIS-Elements BR 4.00.08 software.
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bioRxiv - Cell Biology 2023Quote: ... Measurement of apoptosis and necrosis was carried out following the instructions from the Apoptosis/Necrosis Assay Kit from Abcam. Supernatant from the treated THP-1 macrophages was collected ...
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bioRxiv - Microbiology 2023Quote: ... 2 x 106 parasites were collected and incubated with 200ul FITC conjugated VSG2WT antisera (FITC conjugation kit, Abcam ab102884) (1:200 ...
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bioRxiv - Neuroscience 2023Quote: ... cells grown in the same 96 well plates were stained using Janus Green cell normalization stain kit (Abcam, # ab111622). OD value at 595 nm was measured by Labsystems Multiskan Bichromatic plate reader (Titertek Instruments ...
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Development of monoclonal antibody-based blocking ELISA for detecting SARS-CoV-2 exposure in animalsbioRxiv - Microbiology 2023Quote: ... Biotinylation of the mAb was performed using a Biotin Conjugation Kit by following the manufacturer’s instruction (Abcam, Cambridge, MA). The SARS-CoV-2 N-specific mAb B6G11 was previously developed in Diel lab (20).
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... DNA isolation of treated cells was done as per the manufacturer’s instructions stated in Apoptosis DNA Ladder Assay kit purchased from Abcam. After the isolation of genomic DNA from cells exposed to varying doses of personal care products ...
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bioRxiv - Immunology 2023Quote: A TUNEL assay to determine apoptosis was performed using an in situ Cell Death Detection kit (Abcam, Cambridge, UK). For this assay ...
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bioRxiv - Cancer Biology 2023Quote: ... Cell suspensions were centrifuged and resuspended in calcium-rich binding buffer provided by the apoptosis staining kit (Abcam, #ab14085) to reach ~106 cells/mL ...
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bioRxiv - Cell Biology 2023Quote: ... The efficiency of depletion was assayed by measuring intracellular ATP levels using Luminescent ATP Detection Assay Kit (Abcam ab113849) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... The tilter of the sgRNA lentiviral library was determined by RT-qPCR using the qPCR Lentivirus Titer Kit (Abcam) following the protocol provided by the manufacturer ...
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bioRxiv - Microbiology 2023Quote: We used a Cellular ROS Assay Kit (Red) (ab186027) to measure reactive oxygen species (ROS) in our biofilm samples following the manufacturer’s protocol (Abcam). A 25-μL (OD600=0.1 ...
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bioRxiv - Microbiology 2023Quote: ... Cell lines were validated by phenotypic screening and confirmed to be mycoplasma-free using a PCR detection kit (Abcam). All cells were maintained at 37 °C in a 95% humidity and 5% CO2 atmosphere.
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bioRxiv - Microbiology 2023Quote: ... The amount of histamine released into the culture supernatant was determined using a histamine detection kit (Abcam, Cambridge, MA) as described previously [19] ...
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bioRxiv - Neuroscience 2023Quote: ... cells grown in the same 96-well plate were stained using the Janus Green Cell Normalization Staining Kit (Abcam). OD values at 595 nm were measured by a Labsystems Multiskan Bichromatic plate reader (Titertek Instruments ...
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LRP1 mediates leptin transport by coupling with the short-form leptin receptor in the choroid plexusbioRxiv - Neuroscience 2023Quote: ... assay was performed on paraffin embedded tissue sections according to the manufacturer’s instructions to detect DNA fragmentation in apoptosis (TUNEL assay kit-HRP-DAB, Cat#: ab206386, Abcam). Briefly ...
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bioRxiv - Bioengineering 2024Quote: ... Intracellular lipid accumulation of all viable clones was quantified with Oil Red O Stain Kit (ab150678; Abcam, Cambridge, UK) on day six (Supplementary Fig ...
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bioRxiv - Genomics 2024Quote: ... and ∼10 µg total protein was used to measure TET activity using the TET Hydroxylase Activity Quantification Kit (Abcam) following the manufacturer’s protocol.
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bioRxiv - Cell Biology 2024Quote: Islet were washed once with PBS and cholesterol labelled according to the Cholesterol Assay kit (cell-based) (ab133116, Abcam). Briefly ...
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bioRxiv - Microbiology 2024Quote: ... cAMP levels in the cells were determined in a plate reader by using fluorescence-based cAMP detection kit (Abcam) according to the manufacturer’s recommendations and normalized to the total protein present in the samples as determined by a BCA protein estimation kit (Pierce) ...
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bioRxiv - Microbiology 2024Quote: The amount of ATP generated in the gastrocnemius muscle samples was measured using the ATP assay kit (Abcam, 83355) described in the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2024Quote: ... Tissue ferrous (Fe2+) and total iron content were determined using the colorimetric Iron Assay Kit (Abcam, Cat. No. ab83366) following the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2024Quote: ... SH-SY5Y cells under respective conditions were stained with cathepsin D and L kits (Abcam, Cat# ab65302 and ab270774) following company protocols ...
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bioRxiv - Cancer Biology 2024Quote: ... The slides were further processed using rabbit specific HRP/DAB (ABC) detection IHC kit according to the manufacturer’s protocol (Abcam). Slides were subsequently scanned on an Akoya Vectra Polaris scanner scanning at ×20 magnification and semi-quantitative analysis of cytoplasmic FASN ...
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bioRxiv - Molecular Biology 2024Quote: ... LDH levels were quantified in cell supernatants using an LDH-Cytotoxicity Assay Kit following manufacturers’ protocol (abcam, United Kingdom). To quantify caspase-3/7 activity ...
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bioRxiv - Physiology 2024Quote: Sphingomyelin levels in mAoECs after treatment with vehicle or wortmannin was determined by a sphingomyelin assay kit (abcam, ab138877) while following manufactures instructions.