Labshake search
Citations for abcam :
3751 - 3800 of 3865 citations for Human Hypoxia inducible factor 3 alpha HIF3A ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2021Quote: Histological analyses of the NAFLD tissues were performed using the H&E Stain Kit (Hematoxylin and Eosin) (ab245880; Abcam, Cambridge, UK) and the Oil Red O Stain Kit (Lipid Stain ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... and deparaffinized in serial baths of xylene and ethanol followed by staining using Mayer hematoxylin and 0.2% eosin (Histolab) or picrosirius red staining kit (Abcam, Cambridge, UK). The stained slides were imaged using an Olympus BX60F microscope with an SC50 camera.
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bioRxiv - Cell Biology 2021Quote: The relative contents of endogenous sphingomyelins in WT and SIRT1 KO mESCs were analyzed using a commercially available Sphingomyelin Assay Kit (Abcam ab133118) per manufacturer’s instruction.
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bioRxiv - Cancer Biology 2021Quote: ... cells were collected by trypsinisation and Annexin V staining was carried out using an Annexin V-iFluor 647 Apoptosis Detection Kit (Abcam, ab219919), following manufac-turer’s instructions ...
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bioRxiv - Cell Biology 2022Quote: ... Sorted satellite cells were treated with UV radiation at different dosages and the comet assay was operated immediately after irradiation through a Comet Assay Kit (Abcam, ab238544) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2022Quote: Grown BEC-organoids were treated with the FA-mix for 4 days and triglyceride levels were measured with a Triglyceride kit (Abcam, ab65336) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... Monoclonal α-GPEET and α-EP IgG were conjugated with Allophycocyanin (APC) fluorescence molecule by APC Conjugation Kit – Lightning Link (Abcam). Cells were stained with α-GPEET or α-EP or both α-GPEET and α-EP to detect the loss of GPEET and EP as the indicator of the presence of non-PCF cells in the induced culture ...
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bioRxiv - Physiology 2022Quote: ... Immunohistochemical staining for MMP13 was performed utilizing the Innovex Universal Animal IHC Kit and a polyclonal rabbit anti-mouse MMP13 antibody (Abcam ab39012). Briefly ...
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bioRxiv - Genetics 2022Quote: ... The mixture was centrifuged at 10000g for 5 min and 5μl of the supernatant was used to measure fumarate levels per instruction from the commercial fumarate kit (Abcam, Cambridge, UK).
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bioRxiv - Physiology 2022Quote: RalGDS RBD Agarose beads were used to selectively pull-down the active form of Rap1 (Rap-GTP) from isolated cardiac mitochondria (Rap1 Activation Assay Kit, Abcam, ab212011). Samples of mitochondria ...
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bioRxiv - Cancer Biology 2022Quote: ... cells were incubated for 1 hour at room temperature with PSR staining solution before rinsing twice in Acetic Acid solution (Picrosirius Red Stain Kit, Abcam, ab150681) and imaging ...
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bioRxiv - Systems Biology 2024Quote: ... The detection of EdU incorporation was performed according to the protocol provided by the EdU proliferation kit (Abcam, ab219801, iFluor 488). In brief ...
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bioRxiv - Cell Biology 2024Quote: The mitochondrial membrane potential (ΔΨm) was measured with the JC-1 mitochondrial membrane potential assay kit (ab113580, Abcam, Cambridge, United Kingdom) according to manufacturer’s instructions ...
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bioRxiv - Cell Biology 2024Quote: ... The supernatant was vacuum dried and the dried lipids were dissolved in 200 μL of the assay buffer supplied by the Cholesterol/ Cholesteryl Ester Assay Kit (Abcam, ab65359). 5 μL of each sample was used for each reaction.
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bioRxiv - Neuroscience 2024Quote: ... Sections measuring 5 μm were then cut using the Leica RM2165 and subsequently stained with Movat’s Pentachrome staining kit (Abcam Cat# ab245884). For H&E staining analysis ...
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bioRxiv - Cancer Biology 2024Quote: ... ROS indicator dye was prepared according to the manufacturer’s protocol using DCFDA/H2DCFDA - Cellular ROS Assay Kit (Abcam, ab113851, Cambridge, UK), and was added to each well ...
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bioRxiv - Neuroscience 2024Quote: ... Cresyl Violet & Luxol Blue staining of brain sections was performed using a commercially available kit according to the protocol provided (Abcam, ab150675). Brain images were taken on a Zeiss Axio Scan Z1 slide scanner.
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bioRxiv - Immunology 2024Quote: ... was performed by flow cytometry and triglyceride release assay was determined in supernatant after low-speed centrifugation (300 g, 10 min) using Triglyceride Assay kit (Abcam ab65336). GMPs were collected in a supernatant of granulocytes (3-5 x 106/ml ...
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bioRxiv - Genetics 2023Quote: ... slides were stained for goblet cells and mucins using an Alcian Blue dye and Nuclear Red Fast counterstain kit (Abcam; ab150662). Quantification of the percent area of Alcian blue staining ...
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bioRxiv - Microbiology 2023Quote: ... The preparation of samples and lactate level measurement procedure were followed as specified in the Lactate Assay Kit (Colorimetric) (ABCAM - ab65331) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: Detection of cytoplasmatic and mitochondrial ROS levels was performed by means of a commercial DCFDA / H2DCFDA - Cellular ROS Assay Kit (abcam, ab113851) and MitoSOX™ Red Mitochondrial Superoxide Indicator (Invitrogen ...
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bioRxiv - Cancer Biology 2023Quote: ... Samples were pre-cleared using the beads provided in the kit and incubated overnight at 4°C with 5 µg of commercially available antibodies against lamin A/C (Abcam, #ab108595), phospho-Ser301 lamin A/C (Affinity ...
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bioRxiv - Cancer Biology 2023Quote: ... Samples were diluted 1:400 in Milli-Q water and lactate concentration was measured using a Lactate assay kit (Abcam, ab65330) according to the manufacturer’s protocol (using 1.5 µL of Enzyme Mix per well) ...
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bioRxiv - Neuroscience 2023Quote: ... and cathepsin D (CatD) endo-peptidase activity was determined using commercial assay kits (Cathepsin B: ab65300, Abcam; Cathepsin D: ab65302, Abcam). Samples were homogenized in Lysis buffer as per the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... Immunohistochemical SP method (according to Fuzhou Maishin immunohistochemical ultrasensitive mouse/rabbit SP IHC ready-to-use kit) was performed to detect the expression of CK2A1 (Abcam 76040), CK2A2 (Invitrogen PA5-109601) ...
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bioRxiv - Molecular Biology 2023Quote: LAL activity was determined in control and VPS35-deficient Hepa1-6 cells using the LysoLive™ Lysosomal Acid Lipase Assay Kit (ab253380, Abcam) according to the manufacturer’s instructions for adherent cells ...
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bioRxiv - Molecular Biology 2023Quote: ... The measurement of Cathepsin L activity was performed following the instructions and the protocol of Magic Red Cathepsin Assays Kit (Abcam, ab270774). Photos were taken using a Floid Cell Imaging Station (Thermo Fisher Scientist) ...
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bioRxiv - Cancer Biology 2023Quote: ... IHC labeling for c-Myc was performed using the DISCOVERY HQ+ Amp kit with primary antibody against c-MYC (1:600, Abcam, ab32072). IHC for EdU and MYC on cells grown on chamber slides was performed by following the protocol described before (48) ...
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bioRxiv - Immunology 2023Quote: ... Thymocytes were isolated from untreated and TBI-treated mice and intracellular pyruvate and lactate levels were measured by absorbance using Pyruvate Assay kit (Abcam, ab65342) or Lactate-Glo™ Assay (Promega ...
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bioRxiv - Genetics 2022Quote: ATPase activity of cohesin was measured using EnzChek Phosphate Assay Kit with purified recombinant proteins depleted of free phosphate using Inorganic Phosphate Binding Resin (Abcam: ab270547). Reactions were assembled with10nM cohesin ...
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bioRxiv - Physiology 2023Quote: ... Immunoprecipitation was accomplished using plate based-beads in the EpiQuik Kit along with HNF4α antibodies (Novus NB100-1783 and Abcam ab181604) to validate enrichment at QPRT ...
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bioRxiv - Neuroscience 2023Quote: ... the cryosections of human brain were mounted on a Polyethylene naphthalate (PEN) Membrane Slide (Zeiss, 415190-9041-000) and then stained with spike protein antibodies using the IHC kit (Abcam, ab210062). After further processing ...
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bioRxiv - Cell Biology 2023Quote: Senescence-associated β-galactosidase (SA-β-gal) was used to detect MSCs senescence using SA-β-gal staining kit (Abcam, AB65351) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2023Quote: Measurement of the oxygen consumption rate (OCR) in hiPSC-derived neurons was performed with the fluorescence-based Extracellular O2 Consumption Assay Kit (Abcam, ab197243) as described previously (Castelo Rueda et al. ...
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bioRxiv - Microbiology 2023Quote: ... Infected cell lines were incubated at 37°C in a carbogen environment for two hours prior to conducting cytotoxicity assays using a lactate dehydrogenase (LDH) kit (Abcam, UK) as previously described(48) ...
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bioRxiv - Molecular Biology 2023Quote: ... The cells were fixed and stained to detect β-galactosidase activity using the Senescence Detection Kit from Abcam (ab65351, Abcam, www.abcam.com).
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bioRxiv - Biochemistry 2023Quote: ... The lipid fraction from 1 million cells per sample was extracted after which total cholesterol was assayed using the Cholesterol/Cholesteryl Ester Quantitation Assay kit (Abcam, ab65359) according to the manufacturer’s recommendations ...
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bioRxiv - Cell Biology 2023Quote: ... These different recombinants were used to measure phospholipase A2 (PLA2) activity following procedures described in the kit manual (Abcam, Cambridge, UK), in the presence of ethanol or P4 (10-5M) ...
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bioRxiv - Cancer Biology 2023Quote: To quantitatively measure intracellular reactive oxygen species in live cell samples we have used cell permeant reagent 2’,7’–dichlorofluorescin diacetate (H2DCFDA) from Cellular ROS Assay Kit (Abcam, ab113851) as per the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: For measuring cellular iron in Fe2+ and Fe3+ states as well as total iron content we have a used a colorimetric Iron Assay Kit system (Abcam, ab83366) in stepwise manner as described in the manufacture’s protocol ...
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bioRxiv - Cell Biology 2024Quote: ... proteins from insoluble fractions were initially processed via filter-aided sample preparation (FASP) protocol using FASP protein digestion kit (Abcam; #ab270519)) and proteins from soluble fractions were processed via standard TMT sample processing protocol (Thermo-Fisher) ...
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bioRxiv - Cell Biology 2024Quote: The activity of Cathepsin L from isolated cardiomyocytes from 6-8 mice (plated as described) was assessed using the Cathepsin L Activity Assay kit from Abcam (ab65306). 50μg of protein from isolated cardiomyocytes were used to assess the degradation of the FR-AFC substrate at 37°C degrees for 2 hours ...
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bioRxiv - Cancer Biology 2024Quote: Alanine transaminase (ALT) and aspartate aminotransferase (AST) activity were measured in EDTA-treated plasma using kits from Abcam (ab105134 and ab138878) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2024Quote: NRVMs were transduced with shCtrl or shAtg7 for 48 hours and the supernatants of the cultures were collected for cytokine array analysis using a rat cytokine array kit (Abcam, # ab133992) according the protocol provided by the manufacturer.
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bioRxiv - Genomics 2024Quote: ... for 10 minutes then fixed by 10% neutral buffered formalin for 10 minutes at RT followed by incubation in propylene glycol for 5 minutes at RT and in heated (60°C) Oil Red O solution for 20 minutes using Oil Red O Stain Kit (Abcam, ab150678) according to the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2024Quote: ... Cells were transduced with the Sh2 or Scr control constructs on 4 DIV and cellular ATP measurements were performed on 18 DIV using a Luminescent ATP Detection Assay Kit (Abcam, ab113849). To block luminescence signal contamination from adjacent wells ...
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bioRxiv - Biochemistry 2024Quote: Glycine concentration in homogenates of HepG2 cells or fibroblasts (control and NKH) was measured by a fluorometric Glycine Assay Kit (Abcam, ab211100).
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bioRxiv - Microbiology 2024Quote: ... cell boundaries of mounted infected tissues including MTB bacterium were stained with the Vizgen Cell boundary Staining Kit (Cat. no. 10400009) in conjunction with a polyclonal MTB antibody (Abcam, ab905) raised in rabbit diluted to 1:100 ...
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bioRxiv - Neuroscience 2022Quote: ... from TSA kit for 10-15’ at RT followed by incubation with goat anti-rabbit 568 (1:500, ab175471, Abcam, Cambridge, UK) in TNB blocking buffer and DAPI (1:1000 in 1x PBS) ...
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bioRxiv - Bioengineering 2022Quote: ... Phenol-red free media surrounding scaffolds for 14 days of hMSC cell culture on scaffolds was pooled and 80 μL of this sample was used with an Alkaline Phosphatase Assay Kit (Abcam, United Kingdom) along with phenol-red free media as a background control (n=6) ...