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Citations for abcam :
3401 - 3412 of 3412 citations for Methyl CpG binding protein 2 MECP2 Human HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: ... Immunoblotting was next performed by incubating the membranes overnight at 4°C with the following primary antibodies: mouse monoclonal anti-mitofusin 2 antibody [6A8] (abcam, ab56889, dilution of 1/1000), rabbit polyclonal anti-mitofusin 2 (abcam ...
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bioRxiv - Biochemistry 2023Quote: ... Fluorescently labelled detection antibodies (AT8: Invitrogen, Cat. No. MN1020, 5 nM; HT7: Invitrogen, Cat. No. MN1000, 2 nM; T181: abcam, Cat. No. ab232849, 5 nM) were added in blocking solution ...
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bioRxiv - Genomics 2023Quote: ... were blocked and pH-calibrated as per the manufacturer’s protocol and 5 μl bed volume of beads were loaded with 2 μg of ChIP antibody (H3K27ac; Abcam-AB4729, H3K115ac; PTM BIO-PTM-170). Beads were added to the solubilized chromatin ...
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bioRxiv - Neuroscience 2024Quote: ... United States).The following day slices were washed 4 × 10 min followed by 2 h incubation in secondary antibody solution containing goat-anti-rabbit Alexa Fluor 488 (1:500; Abcam, ab150077, Waltham, MA, United States), goat-anti-chicken Alexa Fluor 488 (1:500 ...
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bioRxiv - Pathology 2020Quote: ... Primary antibodies were incubated in blocking buffer for 2 h at room temperature (rabbit anti-ACE2 [Abcam #ab15348, used at 1:500 dilution]; mouse anti-GAPDH [Abcam #ab8245, used at 1:3,000 dilution]). Membranes were washed and further incubated with fluorescent dye-conjugated secondary antibodies for 1 h at room temperature (AlexaFluor 647 goat anti-rabbit IgG1 and AlexaFluor 488 goat anti-mouse IgG1 ...
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bioRxiv - Genetics 2021Quote: ... This was centrifuged and supernatant was collected into a new tube and incubated with anti-H3K27 antibody (ab177178, Abcam, 2 μg for 25 μg of chromatin) or anti-H3K4me1 antibody (ab8895 ...
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bioRxiv - Immunology 2024Quote: ... overnight at 4°C and the relevant secondary for 1h at room temperature (Streptavidin-AF488 Invitrogen S11223 at 2 µg/mL final, anti-mouse IgG1-Cy5 Abcam ab136127 at 1.5 µg/mL final). After nuclear staining with DAPI ...
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bioRxiv - Neuroscience 2023Quote: ... 1% bovine serum albumin in PBS with 0.1% Tween20 [PBST]) for one hour at RT and left overnight in the A2AR primary antibody at 4°C (Abcam #ab3461, Lot # GR3431019-2, 1:100) in blocking solution ...
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bioRxiv - Cell Biology 2021Quote: ... Non-specific interactions were blocked for 1 hour with blocking solution (3% BSA, Sigma ref. A9647, 2% donkey serum, Abcam ref. ab7475, 0,3% Triton X-100, PBS). Primary antibodies were diluted in the blocking solution and incubated overnight at 4°C ...
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bioRxiv - Neuroscience 2020Quote: ... Tissue was then rinsed in PBS three times for 10 min each and incubated for 2 hr in secondary antibodies (goat anti-rabbit Alexa Fluor® 405 for the PV antibody, (abcam, Cat# ab175652, RRID AB_2687498, 1:500), goat anti-mouse Alexa Fluor® 594 for the 8-oxo-dG antibody (abcam ...
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bioRxiv - Molecular Biology 2024Quote: ... cell lysates were prepared using SDS sample buffer followed by Western Blotting and antibody probing procedures according to the guidelines of the company for the respective antibodies (anti-PPARα: H-2, sc-398394, 1:1000, Santa Cruz Biotechnology; anti-CPT1α: ab128568, 1:1000, Abcam; anti-ERRα: ab137489, 1:1000, Abcam). Imaging was done using KODAK films or the Amersham Imager 680 equipment.
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bioRxiv - Cancer Biology 2020Quote: ... Antibody shifts were done by incubating the protein/extract with 4uL of antibody (α-Twist: Invitrogen PA5-47824, α-Snail: Santa Cruz E-18, α-Sox9: Abcam ab3667, α-E47: BD Pharmingen 554077) for 15 minutes at 25°C before adding radiolabeled probe ...