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Citations for abcam :
3151 - 3200 of 3412 citations for Methyl CpG binding protein 2 MECP2 Human HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2020Quote: ... Membranes were probed for 1 hour at room temperature and overnight at 4°C with primary antibody (Anti-8-Hydroxy-2’-deoxyguanosine antibody, 1:1000, Abcam) in 3% BSA ...
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bioRxiv - Molecular Biology 2020Quote: ... at room temperature for 2 h and then incubated overnight at 4°C in Blocking Buffer containing primary antibody (anti-mouse PIWIL2/MILI, Abcam Cat# ab36764 ...
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bioRxiv - Immunology 2021Quote: ... Antibody staining was typically conducted on ice for 1 h with rat monoclonal FITC or PE anti-mouse Ly6B.2 (clone 7/4; Abcam), FITC or PE/Cy5 anti-mouse F4/80 (clone BM8 ...
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bioRxiv - Immunology 2020Quote: ... thawed unfixed tissue slices were rinsed with PBS then blocked with blocking solution (PBS supplemented with 2% Bovine Serum Albumin (Roth) and 5% Normal Goat Serum (Abcam)) for 1 hour at room temperature before incubation of mAbs at 5 µg/ml overnight at 4°C ...
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bioRxiv - Cell Biology 2022Quote: ... Laminin alpha-2 was used specifically for costaining and was detected by Alexa Fluor 594 Donkey anti-rat (AB150156; 1:200; Abcam). All sections were mounted in Vectashield (Vector Laboratories ...
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bioRxiv - Microbiology 2023Quote: ... The apical side of Caco-2 cells grown on Transwell inserts were exposed for 3 h to 20 nM Axl inhibitor (R428) (Abcam), 1 μg of anti-Gas6 antibody (R&D Systems) ...
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bioRxiv - Cancer Biology 2024Quote: ... secondary antibodies for another 90 minutes at 37°C and mounted with 4’,6-diamidino-2-phenylindole (DAPI) containing mounting medium (Abcam) as published before14 ...
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bioRxiv - Neuroscience 2022Quote: ... Sections were washed in 1xPBS with 0.05% Triton and incubated for 2 hours at room temperature with a secondary antibody (Abcam, ab150077). Brain slices were washed and mounted in Fluoromount (Sigma) ...
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bioRxiv - Microbiology 2024Quote: ... followed by a 2 hours incubation with respective Horseradish peroxidase-coupled secondary antibodies (anti-mouse IgG ab97023 and anti-rabbit IgG ab97051, Abcam). Luminescence was then detected using SuperSignal West Pico PLUS Chemiluminescent Substrate (Thermo Fisher Scientific) ...
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bioRxiv - Neuroscience 2024Quote: ... Slides were then washed 3X in 0.4% Triton-X-100 TBS for 5 minutes before blocking at room temperature for 2 hours in a solution of 10% Normal Goat Serum (Abcam), 3% BSA (Fisher ...
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bioRxiv - Neuroscience 2024Quote: ... at 4°C for 48 h and then for 2 h at room temperature with the fluorescent secondary antibodies Alexa-555 donkey anti-rabbit (1:1000; Abcam), Alexa-633 donkey anti-mouse (1:250 ...
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bioRxiv - Immunology 2024Quote: ... and were assessed in BMDM and RAW264.7 macrophage cells following exposure to 2-AA at the times indicated and by utilizing the ATP Assay kit (cholorimetric/fluorometric) (#ab83355, Abcam) and the PicoProbe AcCoA assay kit (ab87546 ...
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bioRxiv - Neuroscience 2024Quote: ... for 30 min at room temperature and then blocked in a solution of 0.3% PBS-T and 5% normal donkey serum (NDS) for 2 hours before being incubated with primary antibodies (1°Abs included: chicken anti-GFP [1:1000, Abcam, ab13970] ...
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bioRxiv - Neuroscience 2024Quote: ... groups of wildtype larvae (AB*) at 6 dpf were incubated for 2 hours in 30 mL EM with or without 1 mM cisplatin (Abcam) at 28°C ...
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bioRxiv - Cell Biology 2024Quote: ... samples were incubated for 2 h at room temperature with anti-rabbit IgG Alexa Fluor 488 (150081; Abcam, Boston, USA) and anti-mouse IgG Alexa Fluor 546 (Invitrogen A1003 ...
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Caspase cleavage of Influenza A virus M2 disrupts M2-LC3 interaction and regulates virion productionbioRxiv - Microbiology 2024Quote: Antibodies used in this study were mouse monoclonal anti-IAV Matrix 2 (Abcam, ab5416, WB 1:1000, IF 1:100), rabbit monoclonal anti-LC3B (Novus Biologicals ...
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bioRxiv - Molecular Biology 2024Quote: ... Beads were separated on a magnetic rack and washed once before being resuspended in 100 µl antibody buffer (wash buffer, 0.25% Digitonin and 2 mM EDTA) and 1 µl BRG1 antibody (Abcam, ab110641). Beads were incubated with the antibody over night with gentle shaking at 4 °C ...
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bioRxiv - Genomics 2024Quote: ... These strains bearing single-copy integrations were grown overnight at 30°C in rich medium YEA and used for ChIP with 2 µl of anti-GFP (ab290, Abcam). PCR oligonucleotides detecting central core (cc1&3 ...
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bioRxiv - Immunology 2024Quote: ... and were rinsed with PBS for three times before mounting the coverslips to slides in glycerol mounting medium with 4’,6-diamidino-2-phenylindole (DAPI) (ab188804, Abcam). The mounted samples were observed on a Zeiss LSM 880 inverted laser scanning confocal microscope ...
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bioRxiv - Cell Biology 2023Quote: ... ab199956), Bcl-2 antibody (1:1000, rabbit anti, ab194583), and gp91phox (1:2500, rabbit anti, ab129068) were purchased from Abcam company ...
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bioRxiv - Genetics 2023Quote: ... Cells were washed three times in PBS before the addition of 4′,6-diamidino-2-phenylindole (DAPI) (Abcam, Cambridge, UK) diluted 1:10,000 in H2O ...
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bioRxiv - Developmental Biology 2023Quote: The following antibodies were used for chromatin immunoprecipitation: rabbit anti-NK Homeobox 2-1 (NKX2-1, 1 µg per reaction, ab133737, Abcam) and rabbit anti-CEBPA (C/EBPα ...
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bioRxiv - Molecular Biology 2022Quote: ... The two fractions were split to two for IgG and YTHDF1 IP (2 μ each, rabbit IgG isotype control, Abcam ab37415 ...
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bioRxiv - Microbiology 2022Quote: ... Cells were blocked with 2% BSA in PBS and then incubated with anti- H3Cit antibody (citrulline R2 + R8 + R17, clone EPR20358-120; Abcam) overnight at 4°C ...
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bioRxiv - Cell Biology 2023Quote: ... The membrane was blocked in 5% skim milk for 2 h at room temperature then incubated with primary antibodies (Rabbit monoclonal to ATPB at 1/50000 dilution, Abcam) for 16 h at 4 ℃ ...
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bioRxiv - Microbiology 2023Quote: ROS production of the donor strain was measured by using a 2’,7’–dichlorofluorescein diacetate (DCFDA) kit (abcam®, UK), following the manufacturer’s protocol ...
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bioRxiv - Genomics 2023Quote: ... Liquid was removed on a magnet and beads were resuspended in 50uL ice-cold antibody buffer (wash buffer with 2 mM EDTA and 0.1% BSA) and 1 µL of antibody (anti-SON Abcam #ab121759) was added and incubated upright with gentle nutating overnight at 4 °C ...
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bioRxiv - Cell Biology 2023Quote: ... Blocking was performed with 2% BSA in PBS for 30 min and cells were incubated with phalloidin-iFluor 594 reagent (1:1000; ab176757; Abcam) for 1 hour.
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bioRxiv - Cancer Biology 2023Quote: ... Cells were blocked with goat serum for 2 hours at room temperature and incubated with RAD51(1:500 no. ab133534 Abcam) or yH2AX (1:200 no ...
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bioRxiv - Neuroscience 2023Quote: ... The aCSF included 2,3-Dihydroxy-6-nitro-7-sulfamoyl-benzo(f)quinoxaline (NBQX, Alomone Labs) 20 μM and 2-amino-5-phosphonopentanoate (D-AP5, Abcam) 50 μM to block glutamatergic AMPA and NMDA currents ...
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bioRxiv - Neuroscience 2023Quote: ... were washed with PBS + 0.1% Tween-20 (PBS-T) then incubated with 2 µL of PDI antibody (Abcam, Catalog #ab2792) in 200 µL PBS-T for 10 min (end-over-end rotation ...
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bioRxiv - Developmental Biology 2023Quote: ... Samples were then incubated with the following primary antibodies diluted in blocking buffer at room temperature for 2 hours: anti-OCT4 (1:200, catalog no. ab181557; Abcam); anti-TFAP2C (1:100 ...
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bioRxiv - Neuroscience 2023Quote: D-AP5 (D-(−)-2-Amino-5-phosphonopentanoic acid) and SR 95531 (2-(3-Carboxypropyl)-3-amino-6-(4 methoxyphenyl) pyridazinium bromide) were purchased from Abcam, UK ...
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bioRxiv - Bioengineering 2023Quote: ... and then incubated for 2 hours at room temperature with secondary antibodies in PBS (Donkey anti-Mouse 647 nm 1:1000, Abcam ab15107 ...
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bioRxiv - Genomics 2023Quote: ... The remaining supernatant was transferred to a 2 mL round-bottom tube and incubated with 5 μg/μL of anti-GFP antibody (ab290; Abcam) at 4°C with end-over-end rotation for one hour ...
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bioRxiv - Biophysics 2023Quote: Equimolar mixture of histone octamer and LIN28B DNA (2 µM each) were mixed with 1 µM of Oct4 (abcam ab134876) and 3 µM of Sox2 (50 mM HEPES pH 7.5 ...
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bioRxiv - Molecular Biology 2023Quote: ... The medium in each well was replaced with HBSS with 10 μM DCF-DA (2’,7’-dichlorofluorescein diacetate; D6883, Abcam) or propidium iodide staining solution (P4864 ...
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bioRxiv - Developmental Biology 2023Quote: EdU (5-ethynyl-2′-deoxyuridine) labeling assay was performed according to the instruction of EdU Assay / EdU Staining Proliferation Kit (ab219801, Abcam). Proliferating cells were labeled by injecting 10mg/kg BW EdU to mice intraperitoneally 2 hours before sacrifice ...
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bioRxiv - Developmental Biology 2023Quote: ... BSA (blocking agent) was applied on slides for 35 minutes followed by overnight incubation with primary antibodies [MMP-2 (Abcam) at a dilution of 1:100 ...
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bioRxiv - Neuroscience 2023Quote: ... Slides were incubated in primary antibody in 0.1 M Tris/2% FBS in a humidified chamber overnight at 4°C (SMI-32, Biolegend 801702, 1:250, SATB2, Abcam ab92446 1:250 ...
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bioRxiv - Cell Biology 2023Quote: ... cells were incubated for 2 hours at room temperature with the following primary antibodies: BUB1 (mouse, Abcam, ab54893, 1:400), BUBR1 (rabbit ...
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bioRxiv - Neuroscience 2023Quote: ... sections were rinsed in PBS and incubated for 2 hr at room temperature with secondary antibodies 594-donkey-anti-rabbit (1:50; Abcam) and 488-donkey-anti-chicken (1:50 ...
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bioRxiv - Cell Biology 2023Quote: ... Isolated m6A-enriched RNA was immunoprecipitated a second time using 2 µg of a second anti-m6A antibody (Abcam ab151230). Final cDNA Libraries were prepared from twice immunoprecipitated RNA and fragmented input total RNA using the Smarter® Stranded Total RNA-Seq Kit v2 - Pico Input Mammalian (Takara Bio 634411 ...
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bioRxiv - Cell Biology 2023Quote: ... 2 μg of antibodies ChIP-grade (H3K4me2, Abcam ab32356; H3K4me3, Diagenode C15410003; H3, Abcam ab1791; Normal Rabbit IgG, Abcam ab37415) were used per ChIP and were bound to DiaMag protein G-coated magnetic beads (Diagenode ...
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bioRxiv - Cancer Biology 2023Quote: The 5-ethynyl-2’-deoxyuridine (EdU) incorporation assay was performed using the EdU Proliferation Assay Kit (iFluor 488) (ab219801, Abcam). Cells were seeded onto coverslips and incubated with 10 μM EdU for 4 h at 37°C in a humidified atmosphere with 5% CO2 ...
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bioRxiv - Pathology 2023Quote: ... Sections were washed 3 times for 5-10 min in PBT and incubated for 2 h at RT in secondary antibody and Hoechst (Abcam) in 3% NDS in PBT ...
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bioRxiv - Cell Biology 2024Quote: EA.hy926 cells or EZH2 WT/mutant overexpressing HEK-293 treated with NO donor SNP (500 µM) for 2 hours were processed with the help of a Biotin Switch Assay kit (Abcam) using the standard manufacturer protocol ...
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bioRxiv - Molecular Biology 2023Quote: ... Membranes were blocked with TBST containing 5% skim milk or 5% BSA for 2 hours and incubated overnight at 4°C with the following antibodies: anti-MIF (1:200, ab65869, Abcam), anti-iNOS (1:500 ...
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bioRxiv - Neuroscience 2024Quote: ... The membrane was blocked with 5% skimmed milk and probed with MJFR-14-6-4-2 (Abcam, 1:3000 dilution) and EP1536Y (Abcam ...
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bioRxiv - Cell Biology 2024Quote: ... All slides then were incubated at 4°C overnight with a single IF stain of ACE-2 (1:100, ab15348, Abcam) or ACE-2 (1:100 ...