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Citations for abcam :
2901 - 2950 of 3412 citations for Methyl CpG binding protein 2 MECP2 Human HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: Gamma-irradiated infected and non-infected tissue specimens were homogenized on ice using Dounce Glass Manual Tissue Grinder (Etcon) following the Plasma Membrane Protein Extraction Kit protocol (Cat# ab65400, Abcam). Total protein concentration was quantified using Pierce™ BCA Protein Assay Kit (Thermo Fisher) ...
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bioRxiv - Cell Biology 2023Quote: APOB protein levels in RPE and retinal lysates were measured using an Apo B ELISA Kit (ab230932, Abcam Cambridge, UK). Briefly ...
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bioRxiv - Cancer Biology 2023Quote: ... The respective concentration of each sample was calculated based on the standard curve generated by known protein concentrations and measured optical density (OD) at 450 nm following background subtraction according to manufacturer’s instructions (Abcam; ab277082).
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bioRxiv - Biochemistry 2023Quote: ... The tri-methylated lysine 9 and 27 analogs on H3K9C/C110A and H3K27C/C110A proteins were confirmed by western blot assays using monoclonal anti-Histone H3K9me3 and H3K27me3 antibody (Abcam).
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bioRxiv - Cell Biology 2024Quote: ... The gels were run in MOPS buffer at 200 V for 45 min and stained with Instant- Blue Coomassie protein stain (Abcam).
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bioRxiv - Molecular Biology 2024Quote: ... A total of 3 μg of the peptide was loaded on an SDS–polyacrylamide gel and stained with InstantBlue Coomassie Protein Stain (Abcam). Intensity of the remaining peptide bands were quantified using the ImageJ and plotted.
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bioRxiv - Neuroscience 2024Quote: ... 5mg per sample of lysate was added to 50ul Protein G beads (Cytiva 28951379) pre-bound with 10ul anti-PCB (Abcam 110314 ...
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bioRxiv - Cell Biology 2024Quote: ... Lifted cells were washed 2x with cold dPBS (HyClone, SH30264.02) and incubated with lectins at 10 µg/mL or MBL2 protein (Abcam, ab151947) at 1:50 in 3% bovine serum albumin (BSA ...
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bioRxiv - Cancer Biology 2021Quote: ... Multi-color immunohistochemistry was conducted on a cohort of early-stage non-small cell lung cancer patients as described elsewhere (https://jitc.bmj.com/content/9/2/e001469) with polyclonal rabbit anti-Neutrophile Elastase antibody (Abcam, Cambridge ...
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bioRxiv - Genetics 2021Quote: ... Lysates were precleared and then immunoprecipitated with 2 μl anti-histone H3 (monomethyl K9) antibody (Abcam no. ab9045), 2 μl anti-histone H3 (dimethyl K9 ...
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bioRxiv - Neuroscience 2022Quote: ... Slices were then washed and developed for 2-3 min using 3,3′-diaminobenzidine (DAB) staining kit (Abcam; #ab64238;). Nuclei were counterstained using Haematoxylin (MHS32-Sigma ...
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bioRxiv - Bioengineering 2019Quote: ... diluted to 1.25 μg/mL with 0.1% BSA in PBS-0.1% Tween 20 and a secondary antibody of 2 μL/mL Alexa Fluor 488-anti mouse in 0.1% BSA in PBS-0.1% Tween 20 (Abcam). Nuclei were stained with Hoescht 33342 ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... Purmorphamine (9-Cyclohexyl-N-[4-(4-morpholinyl)phenyl]-2-(1-naphthalenyloxy)-9H-purin-6-amine) was from Abcam. SAG1.3 (3-Chloro-N-[trans-4-(methylamino)cyclohexyl]-N-[[3-(4-pyridinyl)phenyl]methyl]benzo[b]thiophene-2-carboxamide ...
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bioRxiv - Genomics 2021Quote: ... and sheared DNA was added to antibodies coupled to washed magnetic beads (anti-FLI1 2 µg, ab15289, Abcam; anti-ERG 2 µg ...
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bioRxiv - Neuroscience 2021Quote: ... and incubated with primary antibodies diluted in PBS containing 2% NHS overnight (1:500 rabbit anti-Iba1, Abcam, ab178846 ...
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CAP1 and cofilin1 cooperate in neuronal actin dynamics, growth cone function and neuron connectivitybioRxiv - Neuroscience 2020Quote: ... 10% donkey serum and 0.5% NP40 in PBS and stained over night at 4°C with following primary antibodies in 2% BSA and 0.5% NP40 in PBS: rat anti-Ctip2 (1:1,000, Abcam), rabbit anti-Neurofilament200 (1:80 ...
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bioRxiv - Genomics 2022Quote: ... The Rabbit IgG control antibodies for ChIP-qPCR experiments were ab172730 (abcam, GR3298823-2, 2ug per 80ug chromatin), and Normal IgG Rabbit 2729 (Cell Signaling ...
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bioRxiv - Bioengineering 2022Quote: ... n=2-3) samples was performed according to manufacturer instructions of the Alkaline Phosphatase Assay Kit (colorimetric) (abcam).
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bioRxiv - Molecular Biology 2022Quote: ... The slides were then stained for 2 minutes 30 seconds with a DAB substrate kit (Abcam ab 64238) and washed in tap water twice prior to incubating in tap water for 2 hours prior to cover slipping.
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bioRxiv - Genomics 2022Quote: ... and incubated overnight at 4°C in either anti-dsDNA (Abcam, 1:5,000 in 2% BSA in TBST) or anti-5-methyl-cytosine (5mC – Aviva Biosystem clone 33D3 ...
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bioRxiv - Microbiology 2021Quote: ... cells were stained with the primary rabbit polyclonal anti-SARS-CoV-2 spike glycoprotein antibody (1:1000, Abcam) for 1 h at RT or overnight at 4 °C ...
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bioRxiv - Developmental Biology 2022Quote: ... The blastocysts were incubated with primary antibodies for 8-Hydroxy-2-deoxyguanosine (8-OHdG, 1:200; ab48508, Abcam), 8-iso Prostaglandin F2 alpha (PGF2 alpha ...
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bioRxiv - Neuroscience 2019Quote: ... incubated 2 h at room temperature with secondary antibody (1/500 biotinylated goat anti-guinea pig ab6907 Abcam), washed again with PBS ...
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bioRxiv - Developmental Biology 2020Quote: ... Blocking was performed with 2% BSA in 0.5% Triton/PBS and rabbit polyclonal anti-mCherry primary antibody (Abcam) was used in 1:500 dilution overnight at 4°C ...
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bioRxiv - Molecular Biology 2019Quote: ... 2 μg of each sample was subjected to slot-blot for immunoblotting with anti-dsDNA antibody (Abcam, ab27156) to confirm equal DNA loading.
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bioRxiv - Neuroscience 2021Quote: ... Grids were incubated for 2 h in the presence of anti-LRRK2 (1:20 dilution, Abcam, cat. # ab133474) and anti-CD63 antibodies (1:40 dilution) ...
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bioRxiv - Immunology 2021Quote: Sonicated chromatin samples of 20,000 cells per assay were profiled for H3K27ac (abcam, cat: ab4729, lot: GR312651-2) with MOWChIP-seq as described in our previous publications53,58 ...
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bioRxiv - Cell Biology 2019Quote: ... The following antibodies were used for Western blot: rabbit anti-DYNLL1/2(1:3000, clone EP1660Y, Abcam, #ab51603), mouse anti-α7 (1:3000 ...
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bioRxiv - Immunology 2021Quote: ... the cells were marked with 4′,6-diamidino-2-phenylindole (DAPI) (cat. n° ab104139, Abcam, Cambridge, MA, USA), and incubated for 5 min ...
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bioRxiv - Neuroscience 2020Quote: ... mice received intraperitoneal injections (2 mg/kg) of each of the endothelin receptor antagonists BQ-123 (ab141005, Abcam) and BQ-788 (ab144504 ...
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bioRxiv - Cancer Biology 2021Quote: ... USA) and oligodendrocyte lineage transcription factor 2 (OLIG2; clone EPR2673, rabbit monoclonal, dilution 1:50, Abcam, Cambridge, UK).
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bioRxiv - Neuroscience 2021Quote: ... and then blocking solution (PBS supplemented with 2% Bovine Serum Albumin (Roth) and 5% Normal Goat Serum (Abcam)) was applied for 1 hour at room temperature ...
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bioRxiv - Cell Biology 2021Quote: ... for 2 h at 4°C followed by incubation with primary antibody to TCFL5 (1:50; 188075, Abcam) or control IgG (normal rabbit IgG ...
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bioRxiv - Cancer Biology 2021Quote: ... they were then washed and incubated with primary antibodies against Bcl-2 (1: 1000, #7973; Abcam, Cambridge, MA), caspase-3 (1:2000 ...
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bioRxiv - Cell Biology 2022Quote: ... excised and mounted on glass slides with mounting medium containing 4⍰,6-diamidino-2-phenylindole (DAPI) (Abcam, #104139). Descriptive immunofluorescence images were taken using a Leica SP8 confocal microscope at magnification 20x or 40x ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... The fluorescent probe Fura-2/AM and caspase-4 assay kit (fluorometric) were purchased from Abcam (Cambridge, UK). Promega Caspase-Glo™ 3/7 Assay Kit was obtained from Promega Corporation (Madison ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... The concentration of 8-OHdG was then measured using an 8-Hydroxy-2-deoxyguanosine ELISA kit (ab201734, Abcam).
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bioRxiv - Microbiology 2024Quote: Exponentially growing Leptospira (2×109) were used to determine ATP concentration using the luminescent ATP detection assay (Abcam), according to the manufacturer’s instructions.
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bioRxiv - Cell Biology 2024Quote: ... adult animals were placed in fish water containing 10 mM 5-bromo-2’-deoxyuridine (BrdU) (Abcam, Cambridge, UK) overnight 1 and 2 weeks before animals were sacrificed ...
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bioRxiv - Cell Biology 2024Quote: ... at pH 7.2 in water) and homogenized with a Dounce homogenizer with a 2 ml reservoir capacity (Abcam). We performed 20 strokes with a type B pretzel ...
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bioRxiv - Developmental Biology 2023Quote: ... and incubated overnight at 4 ◦C in either anti-dsDNA (Abcam, 1:8000 in 2% BSA in TBST) or anti-5-methyl-cytosine (m5C– Aviva Biosystem clone 33D3 ...
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bioRxiv - Neuroscience 2023Quote: ... Primary astrocyte cultures were loaded with the 5 µM chemical calcium indicator Rhod-2 AM (Abcam, Cat.: #ab142780) in standard artificial cerebrospinal fluid (ACSF ...
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bioRxiv - Neuroscience 2023Quote: For recordings, slices were superfused with oxygenated aCSF (∼2 ml/min, ∼33°C) containing Gabazine (5 µM; Abcam). A bipolar stimulating electrode was placed in the middle of visible barrel fields in layer 4 ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... and incubated overnight at 4°C in either anti-dsDNA (Abcam, 1:5000 in 2% BSA in TBST) or anti-5-methyl-cytosine (5mC – Aviva Biosystem clone 33D3 ...
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bioRxiv - Pathology 2023Quote: ... Sections were next incubated with rabbit alpha-smooth muscle actin (α-SMA) antibody (2 µg/mL, ab5694, abcam) for 30 minutes at room temperature ...
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bioRxiv - Developmental Biology 2023Quote: ... 1 Million cells were used and incubated with 2 µg YAP antibodies (Abcam ab52771, Santa Cruz sc-101199). All following steps were performed as described43,44 ...
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bioRxiv - Cancer Biology 2022Quote: ... the cells were incubated for 2 hours with the anti-MGP primary antibody (Abcam, cat# ab86233, 1:50) diluted in blocking buffer ...
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bioRxiv - Cell Biology 2023Quote: ... All samples were stained with 1 mg/mL 4′,6-diamidino-2-phenylindole (DAPI Staining Solution ab228549, Abcam). Cav-1 (ab2910 ...
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bioRxiv - Cell Biology 2024Quote: ... The following primary antibodies were used: rabbit anti-myosin light chain 2 (1:500) (Abcam, catalog number ab79935) and mouse anti-phospho-myosin light chain 2 (Ser19 ...
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bioRxiv - Cancer Biology 2024Quote: ROS was quantified using a DCFDA (2’,7’-dichlorofluorescin diacetate) assay kit according to the manufacturer’s instructions (Abcam). Briefly ...