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Citations for abcam :
3151 - 3200 of 3321 citations for Rat TNF alpha ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ROS production of the donor strain was measured by using a 2’,7’–dichlorofluorescein diacetate (DCFDA) kit (abcam®, UK), following the manufacturer’s protocol ...
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bioRxiv - Microbiology 2023Quote: ... Reactions were transferred to a 96-well plate and the amount of inorganic phosphate released from the hydrolysis of NTP was determined using Phosphate Assay Kit PiColorLock (Abcam) according to protocol by the manufacturer ...
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bioRxiv - Cancer Biology 2023Quote: We measured mitochondrial membrane potential (MtMP) using the JC-1(tetraethylbenzimidazolylcarbocyanine iodide) mitochondrial membrane potential assay kit (Abcam; Cat #113850) following the manufacturer’s instruction ...
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bioRxiv - Physiology 2023Quote: ... cells were equilibrated to room temperature and stained with PI for 30 minutes at 37°C using Propidium Iodide Flow Cytometry Kit (ab139418; Abcam) according to the manufacturer’s protocol and incubated at 37°C in the dark for 30 minutes ...
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bioRxiv - Cell Biology 2023Quote: ... Protein carbonylation was detected by preparing skeletal muscle lysates from approximately 50 mg of muscle by homogenizing in lysis buffer provided by the Protein Carbonyl Content Assay Kit (Abcam). The resulting supernatants were subjected to western blotting following the manufacturer’s instructions.
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bioRxiv - Microbiology 2023Quote: Cell apoptosis resulting from the treatment with carbonic anhydrase inhibitor – S4 was evaluated using the Annexin V-FITC Apoptosis Staining/Detection Kit (ab14085) according to the manufacturer’s protocol (Abcam Inc.). Briefly ...
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bioRxiv - Cancer Biology 2023Quote: The acetyl-CoA content of the cell culture medium was determined with the Acetyl-CoA Assay Kit (Reference: ab87546, Abcam) according to the manufacturer’s instructions.
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bioRxiv - Physiology 2023Quote: ... Ferric Reducing Antioxidant Power (FRAP) assay of maternal plasma was done according to manufacturer instructions (FRAP Assay Kit, colorimetric, ab234626, Abcam).
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bioRxiv - Molecular Biology 2023Quote: ... Separation of U2OS cellular compartments was performed according to the manufacturer’s instructions with a Standard Cell Fractionation kit (abcam # ab109719). Two different markers were used to assess the purity of each fraction ...
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bioRxiv - Microbiology 2023Quote: Gamma-irradiated infected and non-infected tissue specimens were homogenized on ice using Dounce Glass Manual Tissue Grinder (Etcon) following the Plasma Membrane Protein Extraction Kit protocol (Cat# ab65400, Abcam). Total protein concentration was quantified using Pierce™ BCA Protein Assay Kit (Thermo Fisher) ...
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bioRxiv - Cancer Biology 2023Quote: ... and the cells were lysed in 80 μL of ice-cold buffer from Glutathione Assay Kit (catalog no: ab239709, Abcam). The lysed cells were kept on ice for 10 minutes followed by mixing thoroughly with 20 μL of 5% SSA (sulfosalicylic acid ...
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bioRxiv - Cancer Biology 2023Quote: ... Pyruvate concentration in the centrifuged extracellular medium samples (5 min 18000 g, 4°C) was measured in microplates using Pyruvate Assay kit (Abcam) or as described in 36.
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bioRxiv - Neuroscience 2023Quote: ... Human temporal cortex samples were lysed in CB Cell Lysis Buffer from the Cathepsin B Activity Assay Kit (Abcam, ab65300). Mouse cortex samples were lysed in RIPA buffer (150 mm sodium chloride ...
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bioRxiv - Neuroscience 2023Quote: ... and cathepsin D (CatD) endo-peptidase activity was determined using commercial assay kits (Cathepsin B: ab65300, Abcam; Cathepsin D: ab65302, Abcam). Samples were homogenized in Lysis buffer as per the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... flash frozen in liquid nitrogen and stored at -80°C or further concentrated using a polyethylene glycol (PEG) virus precipitation kit (Abcam) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: ... mouse PGE2 ELISA Kit (ab287802and Cytokine & Chemokine 36-Plex Mouse ProcartaPlexTM Panel 1A (EPX360-26092-901) for Luminex assay were obtained from Abcam and Thermofisher respectively ...
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bioRxiv - Neuroscience 2024Quote: ... GDH activities in primary astrocytes were determined with glutamate dehydrogenase activity assay kit following with the manufactureŕs instructions (Abcam, ab102527).
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bioRxiv - Microbiology 2024Quote: The SOD activity of the gastrocnemius muscle tissues from the mice was assessed using the colorimetric assay kit (Abcam, ab65354) as per the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2024Quote: ... then resuspended at 1×105 cells/mL in ice-cold PBS and analysed using the comet assay kit (Abcam, #ab238544) according to manufacturer’s instructions with gel electrophoresis run for 20 minutes at 3 volts/cm.
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bioRxiv - Physiology 2024Quote: ... an aliquot of whole tissue lysate was used for plasma membrane purification using the Plasma Membrane Purification Kit (ab65400, Abcam) as previously described.40 Concentrations of proteins were measured using the Bradford assay and stored at -80°C until western blotting.
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bioRxiv - Cancer Biology 2024Quote: Matrix metalloproteinase (MMP) activities (as a general enzyme class) were assayed using abcam MMP Activity Assay Kits (Cat # ab112146, Fluorescent [green]; abcam, 152 Grove Street ...
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bioRxiv - Genetics 2024Quote: ... purified Lp(a) preparations were treated with a variety of fluorescent labeling strategies – FITC Conjugation Kit (Abcam, Cambridge UK, #ab188285), Dil (1,1’-Dioctadecyl-3,3,3’,3’-Tetramethylindocarbocyanine Perchlorate ...
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bioRxiv - Cell Biology 2024Quote: Enzymatic activity of secreted LOX was measured in 10 μl cell culture supernatants using the fluorometric Lysyl Oxidase Activity Assay Kit (Abcam). LOX activity kinetics was evaluated by measuring fluorescence (Ex/Em = 535/587 nm ...
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bioRxiv - Pathology 2024Quote: ... microsome were isolated from the livers of control (Mboat7fl/fl) or hepatocyte-specific Mboat7 knockout mice (Mboat7HSKO) using microsome isolation kit from Abcam and protein was quantified using the bicinchoninic assay (Pierce) ...
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bioRxiv - Microbiology 2024Quote: ... Serine racemase treatment of human serum was performed with the addition of 5 µl of proprietary serine racemase solution from a DL-serine assay kit (Abcam) to 1 ml of serum for 3 h ...
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bioRxiv - Cell Biology 2019Quote: The TC and free cholesterol content of hLECs were measured colorimetrically using a Cholesterol assay kit according to the manufacturer’s protocol (#ab65359; Abcam, Cambridge, UK). Harvest the amount of hLECs necessary for each assay (initial recommend = 1 x 107 cells) ...
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bioRxiv - Immunology 2021Quote: ... Intracellular HO-1 staining for human and mouse was performed using the Invitrogen FIX & PERM Kit (GAS003) using HO-1 PE at 1:100 dilution (Abcam, ab83214) and PE IgG2b isotype (Abcam ...
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bioRxiv - Developmental Biology 2021Quote: ... slides were incubated with secondary antibody for 2 h at room temperature before DAB reaction using the DAB detection kit (Abcam #ab64238). Slides were counterstained with hematoxylin to stain nuclei ...
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bioRxiv - Cell Biology 2022Quote: ... NAD+/NADH ratio and the concentration of total NAD+ and NADH was determined by NAD/NADH fluorometric assay kit from Abcam (ab176723) following manufacture’s protocol ...
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bioRxiv - Immunology 2021Quote: ... Staining was completed per manufacturer’s instructions for RNAScope® multiplex V2 detection kit (ACD Bio) coupled with immunofluorescent protein staining for either Cytokeratin19 (ab52625 Abcam) at 1:250 dilution ...
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bioRxiv - Neuroscience 2020Quote: ... Oil Red O (ORO) staining was performed on muscle cryosections (10μm) to visualize neutral lipids using an ORO kit (Abcam, Cambridge, GBR). Briefly ...
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bioRxiv - Bioengineering 2020Quote: Quantification of proteins via C-terminal GFP tags was performed using a GFP quantification kit from AbCam (Cambridge, UK, product # ab171581) according to manufacturer’s instructions ...
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bioRxiv - Neuroscience 2020Quote: ... The general activity of MMPs in iPSC-astrocyte supernatants was determined using the MMP Activity Assay Kit (Abcam, Cat. no.: ab112147) following the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2020Quote: The number of apoptotic cells before and 1h and 4 weeks after music exposure (n=10 in each group) was evaluated with the TUNEL method using the “17- 141 TUNEL Apoptosis Detection Kit” (Abcam, UK) in accordance with the standard protocol provided by the manufacturer and analyzed by confocal microscopy (Olympus FLUOVIEW FV10i-W ...
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bioRxiv - Cancer Biology 2020Quote: ... ChIP for RNA polymerase II was performed according the iDeal ChIP-Seq Kit instructions using the anti-Pol2 antibody (Abcam, ab5408). Immunoprecipitated DNA was bisulfite converted using the EZ DNA Methylation Gold Kit (Zymo) ...
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bioRxiv - Bioengineering 2021Quote: ... Twenty microliters of reagent was added directly to each well plate following the instructions of the MTS Cell Proliferation Assay Kit (colorimetric ab197010, abcam®). The culture plate was incubated for 3 h at 37°C in a humidified atmosphere containing 5% CO2 ...
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bioRxiv - Plant Biology 2021Quote: ... 1 mM dithiothreitol (DTT) and 1% (v/v) protease inhibitor cocktail and then used for protein quantification with BCA protein quantitative Kit (Abcam ab6789). The protein sample (200μg amounts ...
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bioRxiv - Molecular Biology 2021Quote: The activity of mitochondria Complex I was measured in peeled murine esophageal epithelium using Complex I Enzyme Activity Assay kit (Abcam, ab109721) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2020Quote: ... cells were incubated with filipin III (1:100) in PBS for 2 h as described in the manufacturer’s protocol of the cholesterol assay kit (Abcam Biochemicals, ab133116). Filipin III was also added in all following incubation steps with the primary and secondary antibody to enable proper staining ...
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bioRxiv - Cell Biology 2021Quote: Cathepsin B activity was assessed in mouse primary neuronal samples and mouse brain hippocampus using Fluorometric Cathepsin B activity assay kit (abcam, 65300) following the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2021Quote: ... The fixed cells were washed three times with water and then partially lysed with a yeast nuclei isolation kit (Abcam 206997) using the following modified protocol ...
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bioRxiv - Cancer Biology 2022Quote: ... Peroxidase and non-specific protein blocking were done as per the instructions using the Abcam ABC detection kit (ab64264) and incubated with the following primary antibody dilutions: anti-Androgen Receptor (Abcam, ab74272), anti-Ki67 (Abcam ...
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bioRxiv - Microbiology 2022Quote: ... solani IPO 2222 WT strain and ΦD5□resistant Tn5 mutants were isolated using a Lipopolysaccharide Extraction Kit (Abcam, Symbios, Gdansk, Poland) with a modified protocol (Bartnik et al. ...
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bioRxiv - Immunology 2022Quote: ... Alanine liberated was measured as increasing fluorescence signal (Resorufin, Ex/Em: 535/587 nm) recorded at 25 °C using an L-alanine assay kit (Abcam, ab83394) at 25 °C according to manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: ... and CTLA-4 Ig (Abatacept) were generated using the APC Conjugation Kit - Lightning-Link® according to manufacturer’s instructions (Abcam, ab201807). APC conjugated anti-PD-L1 antibodies were procured from Biolegend (clones MIH1 ...
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bioRxiv - Molecular Biology 2022Quote: ... Slides were gently washed in phosphate buffered saline (PBS) prior to blocking with the blocking reagent from the mouse-on-mouse polymer IHC Kit (Abcam ab269452). The tissues were then incubated overnight with the primary antibody at 4°C ...
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Development of a small molecule that corrects misfolding and increases secretion of Z α1-antitrypsinbioRxiv - Pharmacology and Toxicology 2020Quote: ... Immunohistochemistry for polymerised α1-antitrypsin was performed using 2C1 monoclonal primary antibody and a Mouse-on-Mouse Polymer kit (Abcam AB127055) for detection ...
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bioRxiv - Cancer Biology 2020Quote: Mammary glands were dissected and homogenized in NOS assay buffer and further processed following the Nitric Oxide Synthase Activity Assay kit (Abcam, ab211083) protocol for measuring enzymatic activity of nitric oxide synthase (NOS) ...
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bioRxiv - Cancer Biology 2019Quote: ... Nuclear protein sample extract from washed cell pellets was carried out according to the manufactures protocol using cell fraction kit – standard (Abcam, ab109719). Nuclear pellets were resuspended in 100mM ammonium bicarbonate and lysed by sonication at 26 joules ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... and deparaffinized in serial baths of xylene and ethanol followed by staining using Mayer hematoxylin and 0.2% eosin (Histolab Products AB, Askim, Sweden) or picrosirius red staining kit (Abcam, Cambridge, UK). The stained slides were imaged using an Aperio CS2 image capture device.