Labshake search
Citations for abcam :
3101 - 3150 of 3321 citations for Rat TNF alpha ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2022Quote: Cell viability of SHANK3-silenced PANC-1 cells treated with ERK and MEK inhibitors were measured using Cell Counting Kit-8 (WST-8 / CCK-8; Cat. no. ab228554, Abcam). Cells were silenced for 24 h as described above and then seeded on a 96-well plate with full medium containing DMSO (control) ...
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bioRxiv - Evolutionary Biology 2022Quote: ... an aliquot of the reaction was used to determine the amount of synthesized ATP using ATP Assay Kit (Colorimetric/Fluorometric) (Abcam). The assay was performed at 37 °C ...
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bioRxiv - Microbiology 2022Quote: Membrane fluidity was measured as previously described (136) using a membrane fluidity kit that quantifies the fluorescence of a lipophilic dye (Pyrenedecanoic acid) (Abcam). Pyrene decanoic acid exists in monomeric and dimeric states within membranes ...
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bioRxiv - Systems Biology 2022Quote: ... Protein Assay Kit (Thermo-Fisher) and the activation of the c-Jun transcription factor was assayed by c-Jun Transcription Factor Assay Kit (Abcam) according to the manufacturer’s instruction ...
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bioRxiv - Plant Biology 2022Quote: ... 0.5 µm) were prepared and stained with 1% (w/v) toluidine blue and the Periodic Acid Schiff kit (ab150680, Abcam) as described in Hawkins et al ...
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bioRxiv - Neuroscience 2023Quote: ... Cell viability was assessed after 72 hours using a 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium (MTS) assay kit (Abcam, ab197010) according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2023Quote: Subcellular fractionation of HeLa cells transfected with the ZC3H11A-Flag expressing plasmids (5μg plasmid/100mm tissue culture plate) was done using Cell Fractionation Kit (Abcam, ab109719) according to the manufacturer’s instructions ...
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bioRxiv - Physiology 2022Quote: Quantitative cAMP measurements were carried out on isolated cardiac mitochondria (0.5 mg per sample) using cAMP Direct Immunoassay Kit (Fluorometric) (Abcam, ab138880). Mitochondria samples were treated with 0 or 10 or 15 mM [HCO3−] ...
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bioRxiv - Molecular Biology 2022Quote: ... A TET activity assay was also performed on nuclear extracts using a fluorometric commercial assay (TET Hydroxylase Activity Quantification Kit, catalog# ab156913; Abcam) and microplate fluorometer (Synergy H1 ...
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bioRxiv - Molecular Biology 2022Quote: A DNMT activity assay was performed on nuclear extracts using a commercial assay (Colorimetric DNMT Activity Quantification Kit, catalog# ab113467; Abcam) and microplate spectrophotometer (Synergy H1 ...
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bioRxiv - Molecular Biology 2024Quote: AP-sites were tested in U2OS cells 48 h after transfection with control and UNG siRNA by a DNA damage assay kit (ab211154, Abcam) according to the manufacturer’s instructions.
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bioRxiv - Biochemistry 2024Quote: ... 2 ml of cells were harvested by centrifugation and treated according to the standard protocol (TUNEL Assay Kit – FITC, ab66108, Abcam). Measurement of FITC fluorescence was performed with CytoFLEX cytometer (Beckman Coulter ...
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bioRxiv - Cancer Biology 2024Quote: ChIP experiments were performed in duplicate for WT and MT cells (clones MT1 and MT2) using the High Sensitivity ChIP Kit (Abcam). 5x105 cells were used as input material for each immunoprecipitation ...
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bioRxiv - Cancer Biology 2024Quote: Surface-expressed EREG molecules/cell were quantified by labeling H231 with phycoerythrin (PE) with the Lightning-Link PE conjugation kit (Abcam) and BD QuantiBRITE PE ...
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bioRxiv - Microbiology 2024Quote: ... 5% CO2 for 24 h before supernatants were collected and LDH levels determined using an LDH-Cytotoxicity Assay Kit (abcam).
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bioRxiv - Molecular Biology 2023Quote: SA-bGAL activity was evaluated in the doxorubicin-induced senescent cells after 10 days using the Senescence Detection Kit (Catalogue number: ab6535, Abcam) as per manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2024Quote: Metabolic activity within periosteal MSC was quantified using a Cell Counting Kit 8 assay (WST-8 / CCK8; Abcam, Cambridge, UK). Water soluble tetrazolium salts are used to quantify cell viability through the production of formazan dye similar to that of an MTT assay ...
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bioRxiv - Immunology 2024Quote: ... and were assessed in BMDM and RAW264.7 macrophage cells following exposure to 2-AA at the times indicated and by utilizing the ATP Assay kit (cholorimetric/fluorometric) (#ab83355, Abcam) and the PicoProbe AcCoA assay kit (ab87546 ...
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bioRxiv - Cell Biology 2024Quote: ... adult mouse cardiomyocyte cell line HL-1 or primary neonatal mouse cardiac fibroblasts (NMCF) or cardiomyocytes (NMCM) using the Histone extraction kit (Abcam) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: ... The amount of cholesterol in cells and supernatants was assessed using the cholesterol/cholesteryl ester assay quantification kit (ab65359, abcam) according to the manufacturer’s instructions.
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bioRxiv - Immunology 2024Quote: ... Cellular ROS was also detected using the DCFDA/H2DCFDA cellular ROS assay kit according to the manufacturer’s instructions (Abcam; ab113851). To analyze cell populations ex vivo ...
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bioRxiv - Immunology 2024Quote: ... spent media was collected for each condition and lactate secretion was assayed in duplicate or triplicate using either Lactate Colorimetric/Fluorometric kit (abcam) or Lactate-Glo (Promega ...
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bioRxiv - Microbiology 2023Quote: ... 45 μL of reaction mixtures were added to 100 μL of glutamate detection reaction mix (Abcam Glutamate Assay Kit, ab83389) in a 96-well plate ...
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bioRxiv - Molecular Biology 2022Quote: ... 3’- diaminobenzidine (DAB) were done using the Mouse and Rabbit Specific HRP/DAB IHC Detection Kit - Micro-polymer (ab236466, Abcam). Before the protein block ...
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bioRxiv - Developmental Biology 2022Quote: ATP synthase activity of wild-type and stat3 mutant zebrafish embryos was measured using the ATP Synthase Specific Activity Microplate Assay Kit (Abcam) following the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2023Quote: The activity of lysyl oxidase (LOX) in CM samples (n = 27) was measured using fluorometric commercial assay (Lysyl Oxidase Activity Assay Kit, Abcam) following the manufacturer’s protocol ...
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bioRxiv - Microbiology 2023Quote: ... Caspase activation was assayed with the Caspase-3 and Caspase-1 colorimetric assay kits from abcam (ab39401 and ab273268, respectively). At harvest ...
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bioRxiv - Neuroscience 2023Quote: ... and native histones that contain the modification and were purified from HEK293T cells using a Histone Extraction Kit (Abcam ab113476). Protein concentration was determined using the Pierce BCA assay ...
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bioRxiv - Molecular Biology 2023Quote: ... gel followed by either silver staining with Pierce Silver Stain Kit (Thermo) or Western blotting against anti-NOP58 (#ab236724, Abcam) and anti-GAPDH (#5174s ...
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bioRxiv - Neuroscience 2023Quote: ... were labeled with the fluorescent dye Alexa Fluor 647 according to the manufacturer’s protocol (Alexa Fluor 647 conjugation kit lightning link, Abcam, ab269823). Briefly ...
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bioRxiv - Microbiology 2023Quote: ROS production of the donor strain was measured by using a 2’,7’–dichlorofluorescein diacetate (DCFDA) kit (abcam®, UK), following the manufacturer’s protocol ...
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bioRxiv - Microbiology 2023Quote: ... Reactions were transferred to a 96-well plate and the amount of inorganic phosphate released from the hydrolysis of NTP was determined using Phosphate Assay Kit PiColorLock (Abcam) according to protocol by the manufacturer ...
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bioRxiv - Cancer Biology 2023Quote: ... the quantification of changes in mitochondrial membrane potential was determined following the protocol of the TMRE-Mitochondrial Membrane Potential Assay Kit (Abcam). Briefly ...
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bioRxiv - Neuroscience 2023Quote: ... and cathepsin D (CatD) endo-peptidase activity was determined using commercial assay kits (Cathepsin B: ab65300, Abcam; Cathepsin D: ab65302, Abcam). Samples were homogenized in Lysis buffer as per the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2023Quote: ... Total nitrite/nitrate was measured as a proxy for nitric oxide concentration using the Nitric Oxide Assay Kit (Abcam, ab65327), with 5 hours of incubation with the enzyme cofactor to permit more than 99% conversion of nitric oxide to nitrite.
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bioRxiv - Molecular Biology 2023Quote: ... cell viability was evaluated at the end of incubations by a dehydrogenase activity assay (Cell Counting Kit 8, Abcam #ab228554), and expressed relative to the solvent control.
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bioRxiv - Microbiology 2023Quote: ... A bacterial culture containing about 109 exponentially growing Leptospira was used to determine the catalase activity using the Catalase Activity Assay Kit (Abcam), according to manufacturer’s instructions.
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bioRxiv - Developmental Biology 2023Quote: ... slides were incubated with the polymer rabbit detection system (DCS) and phH3 staining was detected after incubating the slides with DAB substrate kit (Abcam) for 10 min at RT ...
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bioRxiv - Neuroscience 2023Quote: ... Human temporal cortex samples were lysed in CB Cell Lysis Buffer from the Cathepsin B Activity Assay Kit (Abcam, ab65300). Mouse cortex samples were lysed in RIPA buffer (150 mm sodium chloride ...
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bioRxiv - Biochemistry 2022Quote: ... Anti-1D4 Ab and anti-OLLAS Ab were conjugated to PE using an Ab conjugation kit from Abcam (cat. 102918) according to the manufacturer’s protocols ...
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bioRxiv - Cell Biology 2023Quote: The Dihydroethidium (DHE) assay kit for the measurement of intracellular superoxide and hydrogen peroxide was purchased from Abcam (Cat#ab236206). The MitoSOXTM for the measurement of mitochondria superoxide was purchased from ThermoFisher Scientific (Cat#M36008) ...
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bioRxiv - Developmental Biology 2023Quote: EdU (5-ethynyl-2′-deoxyuridine) labeling assay was performed according to the instruction of EdU Assay / EdU Staining Proliferation Kit (ab219801, Abcam). Proliferating cells were labeled by injecting 10mg/kg BW EdU to mice intraperitoneally 2 hours before sacrifice ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... 5 μL of Annexin V-FITC and 5 μL of propidium iodide were added into each tube (Annexin V-FITC Apoptosis Staining/ Detection Kit, Abcam). The tubes were incubated at RT for 5 min in the dark ...
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bioRxiv - Cancer Biology 2023Quote: The 5-ethynyl-2’-deoxyuridine (EdU) incorporation assay was performed using the EdU Proliferation Assay Kit (iFluor 488) (ab219801, Abcam). Cells were seeded onto coverslips and incubated with 10 μM EdU for 4 h at 37°C in a humidified atmosphere with 5% CO2 ...
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bioRxiv - Cancer Biology 2023Quote: Changes in mitochondrial membrane potential produced by I3C and BNF in the five cancer cell lines was determined with the tetramethylrhodamine ethyl ester (TMRE) Mitochondrial Membrane Potential Assay Kit (ab113852, Abcam) following the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2023Quote: RCC243 cells were treated with 5 μM MS023 for the indicated time periods and then cells were trypsinized and stained using the Propidium Iodide Flow Cytometry Kit (Abcam, #ab139418, Abcam) based on the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... Protein carbonylation was detected by preparing skeletal muscle lysates from approximately 50 mg of muscle by homogenizing in lysis buffer provided by the Protein Carbonyl Content Assay Kit (Abcam). The resulting supernatants were subjected to western blotting following the manufacturer’s instructions.
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bioRxiv - Physiology 2023Quote: ... Ferric Reducing Antioxidant Power (FRAP) assay of maternal plasma was done according to manufacturer instructions (FRAP Assay Kit, colorimetric, ab234626, Abcam).
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bioRxiv - Physiology 2023Quote: ... cells were equilibrated to room temperature and stained with PI for 30 minutes at 37°C using Propidium Iodide Flow Cytometry Kit (ab139418; Abcam) according to the manufacturer’s protocol and incubated at 37°C in the dark for 30 minutes ...
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bioRxiv - Cancer Biology 2024Quote: ... non-esterified fatty acids (NEFA) and glycerol were determined using enzymatic kits (Triglyceride SL, Sekisui Diagnostics; NEFA-HR, Wako; glycerol, ab65337 Abcam) according to the manufacturer’s instructions ...