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Citations for abcam :
3051 - 3100 of 3321 citations for Rat TNF alpha ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2019Quote: ... oryzae conidia were harvested and normalized using the Iron Assay Buffer (100 ml) from the Iron Assay kit (Abcam, ab83366), to reach a final conidia count of 3×105/well ...
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bioRxiv - Biochemistry 2019Quote: Cathepsin D activity (used as a proxy for total lysosomal hydrolase activity) was measured by using Cathepsin D Activity Assay Kit (Abcam). Cells were collected ...
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bioRxiv - Cell Biology 2019Quote: Cells treated with 0.1% DMSO or 5 μM SGC-0946 were stained with Annexin V-FITC and DAPI following the protocol of the Annexin V-FITC Apoptosis Detection Kit (Abcam). Fluorescence was detected by FACS using the CyAn ADP Analyzer (Beckman Coulter ...
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bioRxiv - Cancer Biology 2019Quote: ... cells were lysed and the level of PKC-specific kinase activity was measured in 30 μg cell lysate using the PKC Kinase Activity Assay Kit (AbCam) as described by the manufacturer ...
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bioRxiv - Developmental Biology 2019Quote: ... 21 Gclc KO and Gclc WT mice were anesthetized and eyes enucleated and immediately assayed using the GSH/GSSG Ratio Detection Assay Kit (Fluorometric-Green) (Abcam) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2020Quote: ... The culture supernatants containing pseudotyped viruses particles were either used immediately or flash frozen in aliquots and stored at 80°C until use after being concentrated using a PEG virus precipitation kit (Abcam). Incorporation of the SARS-CoV-2 S protein into the SARS-CoV-2pp was confirmed using Western Blot analysis with anti-SARS-CoV-2 S1 chimeric monoclonal antibody with combined constant domains of the human IgG1 molecule and mouse variable regions (40150-D001 Sinobiological ...
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bioRxiv - Bioengineering 2021Quote: ... stained with 10 µl of AnnexinV-FITC and propidium iodide (PI) following manufacturer’s instructions (Annexin-V Apoptosis Staining Detection Kit, ab14084, Abcam, USA). Metabolic activity tests were performed using an alamarBlue cell viability assay (BUF012 ...
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bioRxiv - Biochemistry 2020Quote: ... and M2 activated (n = 3) was reported as fold-change from control using a commercial intracellular ROS fluorescence assay kit (Abcam, used according to manufacturer’s instructions) ...
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bioRxiv - Cancer Biology 2020Quote: The relative MDA concentration in cell or tumor lysates was assessed using a Lipid Peroxidation (MDA) Assay Kit (Abcam, #ab118970) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2020Quote: The amount of ATP released into the medium was measured with an ATP Assay Kit (Fluorometric, ab83355; Abcam, Cambridge, UK) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2021Quote: ... RNA (1 μg) was used to synthesize cDNA from each donor using the high-capacity reverse transcriptase cDNA synthesis kit (Abcam). DAT ...
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bioRxiv - Cell Biology 2021Quote: ... ovalbumin and CD4-Fc fusion proteins were labelled with allophycocyanin (APC) with the Lightning-Link Labelling Kit (Abcam, Cambridge, UK).
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bioRxiv - Microbiology 2021Quote: Culture media and cellular extracts were collected and centrifuged in preparation for LDH activity quantitation with a commercially available kit (#ab102526, Abcam). LDH activity was measured by a microplate reader (Tecan Infinite M200 Pro ...
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bioRxiv - Bioengineering 2020Quote: ... hCMEC/D3 and primary rodent microglia cells were labeled with Cell Tracking Dye Kit - Green - Cytopainter (ab138891, Abcam, Cambridge, UK) and Cell Tracking Dye Kit - Deep Red - Cytopainter (ab138894 ...
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bioRxiv - Immunology 2021Quote: ... the HIV Env 1086.c K160N gp120 protein was conjugated to biotin using the Fast Type A Biotin Conjugation kit (Abcam) and then captured on avidin-labeled fluorescent beads (NeutrAvidin™ ...
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bioRxiv - Cancer Biology 2020Quote: Cytosol and mitochondria were separated from A375 cells 2 hr or 4 hr after treatment using the Mitochondria/Cytosol Fractionation Kit (Abcam) following the manufacturer’s instructions.
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bioRxiv - Cancer Biology 2022Quote: ... cytotoxicity and killing potential was measured by LDH amount in the cell supernatant using the LDH-Cytotoxicity Assay Kit (Ab65393, Abcam) following the manufacturer’s protocol.
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bioRxiv - Systems Biology 2022Quote: ... Enzyme activity was assessed on the basis of glutamate production as measured with a glutamate assay kit (Abcam, Cat# 138883). The IC50 value was estimated from biological quadruplicates with a four-parameter logistic model63 and with the use of JMP pro 15 software (version 15.1.0).
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bioRxiv - Physiology 2022Quote: 10 µg of protein from 4-week-old adrenals were used for measurement with PKA kinase activity kit (ab139435, Abcam).
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bioRxiv - Cancer Biology 2022Quote: Cytotoxicity and apoptosis were assessed using the Annexin V-CF Blue 7-Aminoactinomycin D (7-AAD) Kit (Abcam Cat# AB214663) as per manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2022Quote: ... terminal deoxynucleotidyl transferase (TdT)-mediated dUTP-digoxigenin nick-end labeling (TUNEL) staining was performed using TUNEL assay kit (Abcam, ab206386) according to the manufacturer’s instructions.
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bioRxiv - Immunology 2020Quote: The iNOS recombinant protein (full length) was procured from sigma and Nitric Oxide Synthase activity assay kit was from Abcam. The NOSIP (full length ...
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bioRxiv - Cancer Biology 2021Quote: Cell transformation assays in immortalized HBEC hTERT were performed using the Cell Transformation Assay Kit-Colorimetric (Abcam Cat No. ab235698). Based on manufacturer’s protocol ...
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bioRxiv - Plant Biology 2021Quote: ... SOD isoenzyme activity was measured according to the manufacturer’s instructions in the SOD activity assay kit (Abcam, cat. n. ab65354).
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bioRxiv - Plant Biology 2021Quote: Quantitative measurement of reactive oxygen species was carried out using DCFDA cellular ROS detection assay kit (Abcam, product number AB113851), and the assay was carried out following the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2021Quote: Aconitase activities in cytosolic and mitochondrial fraction of the brain cortex lysates were measured using Aconitase Enzyme Activity Microplate Assay Kit (Abcam) according to manufacturer’s instructions.
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bioRxiv - Cell Biology 2021Quote: ... early apoptosis was assessed by measuring the levels of phosphatidylserine on the cell surface using the Apoptosis/Necrosis Assay Kit (Abcam) (see Supplemental Methods).
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bioRxiv - Cell Biology 2021Quote: Oxidative stress in male and female PTECs was assessed by measuring the intracellular levels of superoxide ion with the Cellular ROS Assay Kit (Red) (Abcam) following the manufacturer instructions ...
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bioRxiv - Microbiology 2021Quote: ... for 2 hours after which the cells were washed and stained with 1x Apoxin Green Indicator and 1x 7-AAD (Apoptosis/Necrosis detection kit, Abcam) for 45 min ...
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bioRxiv - Cell Biology 2022Quote: The enzymatic activity of Cathepsin B was measured by using a Cathepsin B Activity Assay Kit (Fluorometric) (Cat. ab65300, abcam). Briefly ...
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Induction of telomerase in p21-positive cells counteracts capillaries rarefaction in aging mice lungbioRxiv - Molecular Biology 2022Quote: Lung fibrosis was quantified on lung sections stained with Sirius Red (Picro Sirius Red Stain Kit, ab150681, Abcam, Cambridge, UK) using the modified Ashcroft scale (Hübner et al ...
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bioRxiv - Physiology 2019Quote: ... Serum free fatty acid levels were assayed in chow-fed or HFHS-fed mothers at gestational day 16 and in the offspring of chow- or HFHS-fed dams at P10 and 10 weeks of age using a commercially available FFA kit (Abcam).
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bioRxiv - Immunology 2019Quote: ... Supernatants were removed immediately following the assay and lactate production was measured using the L-Lactate Assay Kit (Colorimetric; Abcam).
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bioRxiv - Pathology 2020Quote: Substrate-based fluorometric enzymatic activity assays were performed on fresh whole cell lysates of undifferentiated NPCs using Cathepsin L Activity Assay Kit (Abcam) and Cathepsin B Activity Assay Kit (Abcam).
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bioRxiv - Cancer Biology 2019Quote: PDH activity was measured on whole cell lysates using the Pyruvate dehydrogenase (PDH) Enzyme Activity Microplate Assay Kit (ab109902, Abcam).
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bioRxiv - Molecular Biology 2021Quote: ... the membrane was washed again with 1x and the bound antibody was detected with ECL Western Blotting Substrate kit (abcam) and the chemiluminescence captured by exposure to a Carestream XBT X-ray Film ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... the microsomes expressing the enzymes were purified from the infected Sf9 cells with a Microsome Isolation Kit (Abcam, Cambridge, MA). Microsomes were incubated with an NADPH regeneration system (Promega ...
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bioRxiv - Immunology 2019Quote: ... Histones were prepared directly from cell pellets using a histone extraction kit according to the manufacturer’s instructions (Abcam, Toronto, ON). Disintegrations per minute (DPM ...
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bioRxiv - Cell Biology 2021Quote: Endoplasmic reticulum (ER) was stained in live cells using ER Staining Kit - Red Fluorescence - Cytopainter (ab139482) (Abcam, Cambridge, United Kingdom) following the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2020Quote: ... abemaciclib (1 μM – 8 times 24 hours) or nutlin-3a (10 μM – 8 times 24 hours) were crosslinked and processed using the ChIP kit (ab500, Abcam) following manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2021Quote: ... Cells were harvested after 16 hr of c-Myc activation and the chromatin was isolated with Chromatin Extraction Kit (ab117152, Abcam) according to the manufacture’s protocol ...
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bioRxiv - Microbiology 2021Quote: The viability of Vero E6 and MA104 cells after drug treatment was determined using the Cell Counting Kit 8 (ab228554, Abcam). Briefly ...
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bioRxiv - Immunology 2020Quote: ... and IgM-PE mAb (clone K52 1C3, BioRad Antibodies; conjugated using Lightning-Link® PE Antibody Labeling Kit, Expedeon, Abcam), for 30 min on ice ...
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bioRxiv - Immunology 2021Quote: Glyceraldehyde 3 Phosphate Dehydrogenase Activity was measured by using Glyceraldehyde 3 Phosphate Dehydrogenase Activity Assay kit (Colorimetric) (Abcam®, #ab204732) according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2021Quote: ... 500 µL of supernatant fluid was added to 25 µL of pre-incubated protein G magnetic beads (ChIP-Adembeads ChIP-Adem-Kit, Ademtech SA) with 5 µg of anti-V5 rabbit polyclonal antibody (ab9116, Abcam) in IP buffer (50 mM HEPES-KOH pH 7.5 ...
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bioRxiv - Bioengineering 2021Quote: ... 7′-Dichlorofluorescin diacetate (DCFDA) was used to measure intracellular ROS using the DCFDA Cellular ROS Detection Assay Kit (ab113851, Abcam) according to the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2021Quote: The brain ATP level was measured through an ATP Assay Kit according to the manufacturer’s instructions (Abcam, Catalog No. ab83355)10 ...
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bioRxiv - Neuroscience 2021Quote: Plasma samples were used to determine triglycerides levels with the TG assay kit for quantification (Abcam, ab65336; Cambridge, MA, USA) as previously described (Kalyan-Masih et al. ...
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bioRxiv - Cell Biology 2022Quote: Filipin III stocks were made in ethanol and stored in aliquots at −80 °C according to kit instructions (Abcam, ab133116). When ready for use ...
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bioRxiv - Cancer Biology 2022Quote: ... 10 µg of nuclear extract were used for the TP53 functional assay that was conducted according to the manufacturer’s instructions with the calorimetric p53 Transcription Factor Assay Kit (Abcam, ab207225). In short ...