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Citations for abcam :
2951 - 2997 of 2997 citations for Rat Nitric Oxide Synthase Trafficker NOSTRIN ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2023Quote: RCC243 cells were treated with 5 μM MS023 for the indicated time periods and then cells were trypsinized and stained using the Propidium Iodide Flow Cytometry Kit (Abcam, #ab139418, Abcam) based on the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2024Quote: ... 30mg of protein from scraped cells at day N1 and N14 were analyzed with the Cathepsin D Activity assay kit (ab65302; Abcam, Waltham, MA) following the manufacturer’s instructions.
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bioRxiv - Cell Biology 2024Quote: ... None of the cultures or treatments showed signs of cell cytotoxicity using the using the LDH-Cytotoxicity Assay Kit II (Abcam, cat. #ab65393) to analyze 50 ul fresh culture media (not shown) ...
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bioRxiv - Physiology 2024Quote: Mitochondrial membrane potential was measured in γY4KD cells with Pparγ silenced with siRNA and their scrambled control (Scrl) using Mitochondrial Membrane Potential Assay kit (Abcam, Cat# ab113852). Cells were plated on collagen coated 24-well plate at density of 50,000 cells/well (5 well/per group) ...
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bioRxiv - Molecular Biology 2023Quote: A pair of parotid salivary glands were harvested from each mouse (n=5/group) and directly placed in 500 uL of Lactate Assay Buffer from the L-Lactate Assay Kit (ab65331, Abcam, Cambridge, UK), 10 uL of protease inhibitor (PI ...
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bioRxiv - Cancer Biology 2023Quote: ... end-stage bulk tumor and 4- to 15-weeks control HDTVi liver protein lysates (50 μg) were analyzed using Fluorometric Caspase-1 Assay Kit (Abcam, cat.no. ab273268) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: D2HG levels were measured in adherent cultures of 1 x 107 chondrosarcoma cells treated with increasing concentrations of Enasidenib for 48 hours using the D2HG colorimetric Assay Kit (Abcam, Cambridge, UK) following the manufactureŕs recommendations.
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bioRxiv - Cell Biology 2024Quote: ... MockIP and WCL were aliquoted into wells in a 96-well plate and assayed using a fluorometric Cathepsin D activity assay kit (Abcam. Cat# Ab65302) that is based on quantifying the hydrolysis of the preferred cathepsin-D substrate sequence GKPILFFRLK(Dnp)-D-R-NH2 ...
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bioRxiv - Cancer Biology 2024Quote: ... the adherent monolayers were incubated with 100 µl/well cell lysis buffer from the cAMP Direct Immunoassays Kit (Fluorometric, ab138880, Abcam, Cambridge, UK), while the gliomaspheres were collected ...
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bioRxiv - Pathology 2024Quote: ... Masson’s trichrome staining was performed to assess endometrial fibrosis in sections within a 20 μm distance of the FOXA2-assayed sections using a kit (ab150686, Abcam; Waltham, MA) according to manufacturer instructions.
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bioRxiv - Neuroscience 2024Quote: ... The activity of cytosolic- or secretory-phospholipase-A2 was measured according to the procedures described in the kit manuals (Abcam, Cambridge, UK).
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bioRxiv - Immunology 2021Quote: ... Free fatty acid concentration in plasma (diluted 1:25) of clamped mice was determined using a quantitative Free Fatty Acid Assay Kit (Abcam, Cambridge, Great Britain). Absorbance was measured with the Filter Max F5 device (Molecular Devices ...
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bioRxiv - Cell Biology 2019Quote: ... we used the Maxpar Antibody Labeling Kit (Fluidigm) according to manufacturer’s instructions for addition of the respective metal tags: Axin2 (145-Nd, Abcam, ab32197, 2 µg/ml), p-MEK1/2 [S217/221] (151-Eu ...
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bioRxiv - Neuroscience 2020Quote: ... sections were preincubated (1-hour) at RT in a protein blocking solution (Expose Rabbit-Specific HRP/DAB detection IHC Kit, Abcam, Cambridge, UK, ab80437). Then ...
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bioRxiv - Microbiology 2021Quote: ... felis C4 butanol extract were measured with 2’,7’-dichlorofluorescein diacetate (DCFDA) following the manufacturer’s instructions (DCFDA/H2DCFDA Abcam cellular ROS assay kit). Briefly ...
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bioRxiv - Molecular Biology 2022Quote: ... The cells were rinsed with phosphate-buffered saline (PBS) and then stained in the culture well using the Adipogenesis Assay Kit (cell-based, Abcam Catalog No. ab133102). The dye was eluted using the kit’s elution buffer and quantified using a microplate reader at 490 nm wavelength ...
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bioRxiv - Immunology 2022Quote: ... media was collected and LDH activity measurement was carried out in 96-well plate (Costar, cat no. 3370) according to LDH assay kit (Abcam, cat. no ab102526). Absorbance measurement at 450 and 570 nm was carried out using microplate reader (BioTek ...
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bioRxiv - Cancer Biology 2019Quote: ... Cells were collected after 24 h and the caspase activity was determined using the Caspase-3 Assay Kit (Colorimetric) (Abcam ab39401, Cambridge, UK).
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bioRxiv - Molecular Biology 2021Quote: ... The serum is at the top layer and was collected for subsequent colorimetric analysis of the total cholesterol using the Cholesterol/ Cholesteryl Ester Assay Kit (Abcam, Cambridge, MA, USA) following manufactures protocol.
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bioRxiv - Physiology 2020Quote: ... Nuclear sirtuin 1 content and activity was assessed using 10 µg (5 μL) of isolated nuclear protein fractions through a fluorometric assay (Sirt1 Activity Assay Kit, #ab156065, Abcam, Whitby, Ontario, Canada). Another set of samples weighing 5-10 mg were homogenized in RIPA buffer (150 mM sodium chloride ...
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bioRxiv - Microbiology 2021Quote: ... while 100 μg of anti-SARS-CoV-2 S were conjugated with Alexa Fluor® 488 Lightning-Link® Conjugation Kit (Abcam # ab236553). Each experiment was repeated with similar results using anti-SARS-CoV-2 N rabbit polyclonal Ab (GeneTex # GTX135357 ...
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bioRxiv - Microbiology 2021Quote: ... A 100 μg aliquot of anti-SARS-CoV-2 N human mAb was conjugated with Alexa Fluor® 647 Lightning-Link® Conjugation Kit (Abcam # ab269823), while 100 μg of anti-SARS-CoV-2 S were conjugated with Alexa Fluor® 488 Lightning-Link® Conjugation Kit (Abcam # ab236553) ...
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bioRxiv - Neuroscience 2023Quote: ... Triton X-100 in 1X PBS for 20 min and then endogenous biotin was blocked using Avidin/Biotin blocking kit (Abcam; cat. no. ab64212) to reduce non-specific background ...
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bioRxiv - Physiology 2023Quote: ... Unless specified below, all reagents were from MilliporeSigma (St. Louis, MO, USA). LDH assay kit (catalog no. ab65393) was from Abcam (Cambridge, MA, USA). P ...
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bioRxiv - Molecular Biology 2023Quote: The main pro-inflammatory and anti-inflammatory cytokines in blood and heart were measured by corresponding ELIZA kit: tumor necrosis factor-ɑ (TNF-ɑ, Abcam, ab100747), interleukin-1β (IL-1β ...
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bioRxiv - Cell Biology 2023Quote: The chymotrypsin-like activity of the 20S proteasome was assessed using a commercially available fluorometric proteasome activity assay kit (Abcam, catalog number: ab107921) according to the manufacturer’s guidelines ...
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bioRxiv - Cell Biology 2022Quote: Chromatin accessibility in isolated cardiac fibroblasts or whole heart from C57BL/6J mice was measured using the Chromatin Accessibility Assay Kit (Abcam, cat. no. ab185901) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2024Quote: The amount of collagen in liver tissues was determined by measuring the content of hydroxyproline using a Hydroxyproline assay kit (Abcam, Cat#MAK008-1KT) as described by the manufacturer ...
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bioRxiv - Molecular Biology 2023Quote: Three 5 µm sections taken at 150 µm intervals were used for analysis from each animal and were stained with hematoxylin and eosin using the reagents provided in Oil Red O Stain Kit (Abcam, Waltham, MA, USA) for hematoxylin and eosin purchased from StatLab (StatLab ...
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bioRxiv - Bioengineering 2023Quote: LB samples that were autoclaved or not autoclaved were subjected to quantification of NADH/NAD+ using an NAD+/NADH Colorimetric Assay Kit (ab65348, Abcam, Cambridge, United Kingdom). Autoclaved samples were measured before and after either chronoamperometry or cycle voltammetry treatment ...
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bioRxiv - Cell Biology 2023Quote: ... and the quantification of intracellular and extracellular total cholesterol and its fractions was measured using the Cholesterol Assay Kit (Abcam, Cambridge, United Kingdom) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2024Quote: ... Paraffin-embedded slides were also used for the TUNEL assay and processed according to manufacturer instructions (Abcam, TUNEL Assay Kit - HRP-DAB ab206386). The prepared sections were analyzed using a Nikon Eclipse 80i microscope ...
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bioRxiv - Biochemistry 2021Quote: A total of 50 μl of conditioned medium from ARPE-19 cell cultures were collected and used to quantify free fatty acids using the Free Fatty Acid Quantification Assay Kit (Colorimetric) (Abcam cat. # ab65341; Cambridge, MA) following manufacturer’s instructions.
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bioRxiv - Immunology 2022Quote: ... Transiently transfected cells were stained the following day with mAbs conjugated using DyLight® 650 Conjugation Kit (Fast) - Lightning-Link (Abcam, catalog no. ab201803) according to manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... Protein carbonyl residues in were quantified in 10 μg of the cell lysates using the Protein Carbonyl Assay Kit (Abcam, Boston, MA, USA, ab178020). Derivatization and neutralization were carried out according to the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2023Quote: ... Antibodies were fluorescently labelled with AF647 or AF488 using the Alexa Fluor® Conjugation Kit (Fast) - Lightning-Link (Abcam, Cat. No. ab269823 and ab236553) according to manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: Tissue sections were stained for β-galactosidase using a Senescence Detection Kit (ab65351) according to the directions of the supplier (Abcam, Inc., Waltham, MA).
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bioRxiv - Cell Biology 2023Quote: ... To be included antibodies had to be available in a protein free format (except for GPS2, which we purified using an Abcam Protein G purification kit). All antibodies selected were known to be specific ...
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bioRxiv - Molecular Biology 2023Quote: The sample fractionation by protein size was conducted through an eight-channel GELFrEE® 8100 Fractionation System (GELFrEE® 8100 Fractionation System, 8% Cartridge Kit, #42103, Abcam, Cambridge, UK). The cartridge was prepared according to the manufacturer instructions ...
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bioRxiv - Genomics 2024Quote: ... Hematoxylin and eosin (H&E) and Trichrome staining was performed using standard protocols using and H&E and Trichrome Stain Kits (Abcam, ab245880 and ab150686 respectively). The processed sections were visualized using a Leica DM5500 microscope objective magnifications ...
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bioRxiv - Neuroscience 2024Quote: The intracellular levels of ROS/RNS in different immune cells of the CNS were evaluated using the Cellular ROS/RNS Detection Assay Kit (Abcam, USA; catalog number: ab139473) according to the manufacturer’s instructions ...
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bioRxiv - Physiology 2024Quote: ... was measured in plasma and brain homogenate by quantifying the adduct generated when MDA in the sample reacts with thiobarbituric acid (TBA) (Abcam, ab118970, Lipid-Peroxidation Kit).
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bioRxiv - Neuroscience 2020Quote: ... NAD and NADH extractions were performed on 20mg PBS washed fresh tissue on wet ice homogenised with a Dounce homogeniser with 400uL of NADH/NAD extraction buffer (Abcam NAD/NADH Assay kit-ab65348). For details ...
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bioRxiv - Neuroscience 2023Quote: ... samples were incubated for 1 hour in the antibody incubation buffer (Massive Photonics, Buffer kit) with anti-guinea pig AlexaFluor 405 (Abcam, cat ab175678, 1:1000 dilution), phalloidin AlexFluor 488 (Thermo Fisher ...
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bioRxiv - Microbiology 2024Quote: ... Anti- NA antibodies (2E01) or anti-HA antibodies (CR8033 and CR8071) were previously biotinylated using biotin conjugation kit (Abcam, Fast, Type A, Lightning-Link®). Plates were washed again in PBS-T (0.05% Tween-20 in PBS ...
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bioRxiv - Microbiology 2020Quote: ... At 24 h post-infection flow cytometry was performed targeting NP: cells were processed with the BD Cytofix/Cytoperm™ Kit (catalog no. 554714) and stained with anti-NP antibody (Abcam, clone 9G8, catalog no. ab43821) followed by donkey anti-Mouse IgG Alexa Fluor 488 (Invitrogen catalog no ...
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Neuron-astrocyte metabolic coupling facilitates spinal plasticity and maintenance of persistent painbioRxiv - Neuroscience 2022Quote: 10 µl of the supernatant obtained after the first centrifugation at 18000g from the Glycogen assay where combined with 90 µl of Lactate assay Buffer (Abcam® L- Lactate Assay kit, #ab65331) and separated in two wells of a 96 well plate for sample and sample background control ...