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Citations for abcam :
2901 - 2950 of 2997 citations for Rat Nitric Oxide Synthase Trafficker NOSTRIN ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2023Quote: ... Immunohistochemical SP method (according to Fuzhou Maishin immunohistochemical ultrasensitive mouse/rabbit SP IHC ready-to-use kit) was performed to detect the expression of CK2A1 (Abcam 76040), CK2A2 (Invitrogen PA5-109601) ...
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bioRxiv - Neuroscience 2023Quote: ... 300μg of protein was used for immunoprecipitation assay using immunoprecipitation kit containing Dynabeads-coupled protein G (magnetic beads) and human anti-MMP9 (1:700) (Abcam, ab38898). Briefly the protein was mixed with anti-MMP9 antibody and incubated overnight at 4°C ...
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bioRxiv - Molecular Biology 2023Quote: LAL activity was determined in control and VPS35-deficient Hepa1-6 cells using the LysoLive™ Lysosomal Acid Lipase Assay Kit (ab253380, Abcam) according to the manufacturer’s instructions for adherent cells ...
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bioRxiv - Molecular Biology 2023Quote: ... The measurement of Cathepsin L activity was performed following the instructions and the protocol of Magic Red Cathepsin Assays Kit (Abcam, ab270774). Photos were taken using a Floid Cell Imaging Station (Thermo Fisher Scientist) ...
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bioRxiv - Cancer Biology 2023Quote: ... IHC labeling for c-Myc was performed using the DISCOVERY HQ+ Amp kit with primary antibody against c-MYC (1:600, Abcam, ab32072). IHC for EdU and MYC on cells grown on chamber slides was performed by following the protocol described before (48) ...
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bioRxiv - Immunology 2023Quote: ... Thymocytes were isolated from untreated and TBI-treated mice and intracellular pyruvate and lactate levels were measured by absorbance using Pyruvate Assay kit (Abcam, ab65342) or Lactate-Glo™ Assay (Promega ...
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bioRxiv - Genetics 2022Quote: ATPase activity of cohesin was measured using EnzChek Phosphate Assay Kit with purified recombinant proteins depleted of free phosphate using Inorganic Phosphate Binding Resin (Abcam: ab270547). Reactions were assembled with10nM cohesin ...
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bioRxiv - Physiology 2023Quote: ... Immunoprecipitation was accomplished using plate based-beads in the EpiQuik Kit along with HNF4α antibodies (Novus NB100-1783 and Abcam ab181604) to validate enrichment at QPRT ...
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bioRxiv - Neuroscience 2023Quote: ... the cryosections of human brain were mounted on a Polyethylene naphthalate (PEN) Membrane Slide (Zeiss, 415190-9041-000) and then stained with spike protein antibodies using the IHC kit (Abcam, ab210062). After further processing ...
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bioRxiv - Cell Biology 2023Quote: Senescence-associated β-galactosidase (SA-β-gal) was used to detect MSCs senescence using SA-β-gal staining kit (Abcam, AB65351) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... was incubated at 40C overnight followed by application of a rabbit-specific HRP/3-amino-9-ethylcarbazole (AEC) IHC Detection Kit - Micro-polymer (abcam, ab236468) incubated at room temperature for 1 hr ...
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bioRxiv - Neuroscience 2023Quote: Measurement of the oxygen consumption rate (OCR) in hiPSC-derived neurons was performed with the fluorescence-based Extracellular O2 Consumption Assay Kit (Abcam, ab197243) as described previously (Castelo Rueda et al. ...
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bioRxiv - Microbiology 2023Quote: ... Infected cell lines were incubated at 37°C in a carbogen environment for two hours prior to conducting cytotoxicity assays using a lactate dehydrogenase (LDH) kit (Abcam, UK) as previously described(48) ...
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bioRxiv - Molecular Biology 2023Quote: ... The cells were fixed and stained to detect β-galactosidase activity using the Senescence Detection Kit from Abcam (ab65351, Abcam, www.abcam.com).
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bioRxiv - Biochemistry 2023Quote: ... The lipid fraction from 1 million cells per sample was extracted after which total cholesterol was assayed using the Cholesterol/Cholesteryl Ester Quantitation Assay kit (Abcam, ab65359) according to the manufacturer’s recommendations ...
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bioRxiv - Cell Biology 2023Quote: ... These different recombinants were used to measure phospholipase A2 (PLA2) activity following procedures described in the kit manual (Abcam, Cambridge, UK), in the presence of ethanol or P4 (10-5M) ...
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bioRxiv - Cancer Biology 2023Quote: To quantitatively measure intracellular reactive oxygen species in live cell samples we have used cell permeant reagent 2’,7’–dichlorofluorescin diacetate (H2DCFDA) from Cellular ROS Assay Kit (Abcam, ab113851) as per the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: For measuring cellular iron in Fe2+ and Fe3+ states as well as total iron content we have a used a colorimetric Iron Assay Kit system (Abcam, ab83366) in stepwise manner as described in the manufacture’s protocol ...
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bioRxiv - Cell Biology 2024Quote: ... proteins from insoluble fractions were initially processed via filter-aided sample preparation (FASP) protocol using FASP protein digestion kit (Abcam; #ab270519)) and proteins from soluble fractions were processed via standard TMT sample processing protocol (Thermo-Fisher) ...
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bioRxiv - Cell Biology 2024Quote: The activity of Cathepsin L from isolated cardiomyocytes from 6-8 mice (plated as described) was assessed using the Cathepsin L Activity Assay kit from Abcam (ab65306). 50μg of protein from isolated cardiomyocytes were used to assess the degradation of the FR-AFC substrate at 37°C degrees for 2 hours ...
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bioRxiv - Cancer Biology 2024Quote: Alanine transaminase (ALT) and aspartate aminotransferase (AST) activity were measured in EDTA-treated plasma using kits from Abcam (ab105134 and ab138878) according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2024Quote: ... for 10 minutes then fixed by 10% neutral buffered formalin for 10 minutes at RT followed by incubation in propylene glycol for 5 minutes at RT and in heated (60°C) Oil Red O solution for 20 minutes using Oil Red O Stain Kit (Abcam, ab150678) according to the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2024Quote: The activity of CatB was examined in the cortex of 3-month-old mice or human fibroblasts using a Cathepsin B Activity Assay Kit (Abcam, #ab65300). The tissue or fibroblasts were homogenised in CB lysis buffer and then incubated on ice for 30 minutes before being centrifuged at 15,000 x g for 5 minutes at 4°C ...
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bioRxiv - Biochemistry 2024Quote: ... Cells were transduced with the Sh2 or Scr control constructs on 4 DIV and cellular ATP measurements were performed on 18 DIV using a Luminescent ATP Detection Assay Kit (Abcam, ab113849). To block luminescence signal contamination from adjacent wells ...
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bioRxiv - Biochemistry 2024Quote: Glycine concentration in homogenates of HepG2 cells or fibroblasts (control and NKH) was measured by a fluorometric Glycine Assay Kit (Abcam, ab211100).
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bioRxiv - Microbiology 2024Quote: ... cell boundaries of mounted infected tissues including MTB bacterium were stained with the Vizgen Cell boundary Staining Kit (Cat. no. 10400009) in conjunction with a polyclonal MTB antibody (Abcam, ab905) raised in rabbit diluted to 1:100 ...
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bioRxiv - Neuroscience 2022Quote: ... from TSA kit for 10-15’ at RT followed by incubation with goat anti-rabbit 568 (1:500, ab175471, Abcam, Cambridge, UK) in TNB blocking buffer and DAPI (1:1000 in 1x PBS) ...
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bioRxiv - Bioengineering 2022Quote: ... Phenol-red free media surrounding scaffolds for 14 days of hMSC cell culture on scaffolds was pooled and 80 μL of this sample was used with an Alkaline Phosphatase Assay Kit (Abcam, United Kingdom) along with phenol-red free media as a background control (n=6) ...
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bioRxiv - Immunology 2021Quote: ... then were fixed with 4% paraformaldehyde (PFA) for 15 min at room temperature and stained using the Senescence-Associated β-Galactosidase Staining Kit (Abcam ab65351), following the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2020Quote: The glutamate concentration in the conditioned medium of primary microglial cultures was assayed with the glutamate colorimetric assay kit from Abcam (no. 83389), according to the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2020Quote: Quantification of FabI via a C-terminal GFP tags was performed using a GFP quantification kit from AbCam (Cambridge, UK, Cat # ab171581) according to manufacturer’s instructions.
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bioRxiv - Neuroscience 2020Quote: Indirect assessment of glutamate uptake by resting iPSC-astrocyte lines was done using a colorimetric glutamate assay kit following the manufacturer’s recommendations (Abcam; Cat. no.: ab83389). Briefly ...
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bioRxiv - Cancer Biology 2019Quote: ... Protein lysates from each subcellular fraction (cytoplasm, mitochondria, and nucleus) were obtained from PNT1 and LNCaP cells using the cell fractionation kit (Abcam, VIC, Australia) according to the manufacturer’s protocol.
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bioRxiv - Microbiology 2022Quote: Enzyme activity in vaginal swab supernatants and cell pellets was quantified using the α-Glucosidase Activity Assay Kit (Abcam, Waltham, MA, USA), Enzchek Ultra Amylase Kit (Invitrogen ...
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bioRxiv - Neuroscience 2021Quote: ... Conjugation to FITC or ammonium peroxidase was carried out using Abcam’s alkaline phosphatase and FITC conjugation kits (Abcam ab102850 and ab102884 respectively) according to the protocols outlined in the manufacturer’s instruction manuals ...
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Adipocyte vesicles: ‘all-in-one’ packages that stimulate tumor mitochondrial metabolism and dynamicsbioRxiv - Cancer Biology 2019Quote: ... The organic phase was evaporated under liquid nitrogen and lipids were resuspended in 50µL of fatty acid assay buffer (Free Fatty Acid Assay Kit, Abcam, Cambridge, MA, USA). FA (contained in 5µL of buffer ...
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bioRxiv - Microbiology 2019Quote: GAPDH activity was measured in the presence and absence of GAs using Glyceraldehyde 3 Phosphate Dehydrogenase Activity Colorimetric Assay Kit (ab204732, Abcam, Cambridge, MA) as per the manufacturer instructions ...
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bioRxiv - Synthetic Biology 2020Quote: ... The concentrations of NAD(H) (total NAD+/NADH) and NADH only were measured by the NAD+/NADH assay kit (Abcam, Cambridge, UK) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2019Quote: ... the cells were stained using the EXPOSE Mouse and Rabbit Specific HRP/DAB Detection IHC kit (Abcam, United States; Cat. no. ab80436). The enzyme expression was visualized with the mouse monoclonal antibody anti-SOD2 (Santa Cruz ...
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bioRxiv - Immunology 2020Quote: ... Followed by capture of SARS-CoV-2 receptor binding domain which was biotinylated using the lightning-link type-A biotinylation kit(Expedeon/Abcam, 370-0005) for 180s at 10ul/min ...
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bioRxiv - Neuroscience 2020Quote: Absolute quantification of sialic acid in different glycoconjugate fractions was performed using the Sialic Acid (NANA) Assay Kit (Ab83375, Abcam, Cambridge, MA) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2022Quote: ... equal number of cells were lysed using assay buffer provided in the alpha KG assay kit (abcam, ab83431, lot no. GR3410863-1). The lysates were then deproteinized using perchloric acid and nutralized using 2M KOH as per manufacturer’s instructions ...
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bioRxiv - Genomics 2022Quote: Cell cycle analysis was performed by propidium iodide (PI) staining following the protocols from the propidium iodide flow cytometry kit (Abcam, Cat. ab139418). Briefly ...
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bioRxiv - Bioengineering 2024Quote: ... 100 ug of anti-SARS-CoV-2/S2 (1A9) antibody was conjugated to A647 using the Lightning-Link Conjugation Kit (Abcam, Cat# ab269823) according to the manufacturer’s protocol.
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bioRxiv - Plant Biology 2023Quote: ... Two microliter aliquots of the supernatants were transferred to wells of a 96-well plate prefilled with 98μl of assay buffer of the ammonia assay kit from AbCam (AbCam Cat # ab102509), and ammonia levels were quantified according to the manufacturer’s guidelines ...
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bioRxiv - Neuroscience 2023Quote: ... serotonin and brain derived neurotrophic factor using commercially available Enzyme linked immunosorbent assay kits according to the instructions of the manufacturer (ABCAM, Cambridge UK)
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bioRxiv - Genomics 2023Quote: The cholesterol assay was carried out according to the manufacturer protocol using the Cholesterol/Cholesterol Ester Assay Kit (ab65359, Abcam, Cambridge, UK) and normalized to total protein content as determined by Bradford Assay.
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bioRxiv - Biochemistry 2023Quote: Right Ventricular Mitochondrial Enrichments: RV mitochondrial enrichments were isolated from the same PAB and control piglets with a mitochondrial isolation kit (Abcam, Cambridge, MA) as previously described(10 ...
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bioRxiv - Bioengineering 2023Quote: ... was dissected from the constructs at the end of maturation time (day 15) and tested via a colorimetric CK Activity Assay Kit (Ab155901, Abcam, Cambridge, UK) according to the manufacturer’s instructions ...
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bioRxiv - Pathology 2023Quote: ... regularly spaced (500 µm) along the rostrocaudal axis were stained with a Prussian blue and nuclear fast red stain kit (Abcam, Cambridge, UK) following the manufacturer’s protocol ...