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Citations for abcam :
2701 - 2750 of 2997 citations for Rat Nitric Oxide Synthase Trafficker NOSTRIN ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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LRP1 mediates leptin transport by coupling with the short-form leptin receptor in the choroid plexusbioRxiv - Neuroscience 2023Quote: ... assay was performed on paraffin embedded tissue sections according to the manufacturer’s instructions to detect DNA fragmentation in apoptosis (TUNEL assay kit-HRP-DAB, Cat#: ab206386, Abcam). Briefly ...
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bioRxiv - Immunology 2023Quote: A TUNEL assay to determine apoptosis was performed using an in situ Cell Death Detection kit (Abcam, Cambridge, UK). For this assay ...
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bioRxiv - Neuroscience 2023Quote: ... cells grown in the same 96-well plate were stained using the Janus Green Cell Normalization Staining Kit (Abcam). OD values at 595 nm were measured by a Labsystems Multiskan Bichromatic plate reader (Titertek Instruments ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... DNA isolation of treated cells was done as per the manufacturer’s instructions stated in Apoptosis DNA Ladder Assay kit purchased from Abcam. After the isolation of genomic DNA from cells exposed to varying doses of personal care products ...
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bioRxiv - Cancer Biology 2023Quote: ... Cell suspensions were centrifuged and resuspended in calcium-rich binding buffer provided by the apoptosis staining kit (Abcam, #ab14085) to reach ~106 cells/mL ...
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bioRxiv - Cell Biology 2023Quote: ... The efficiency of depletion was assayed by measuring intracellular ATP levels using Luminescent ATP Detection Assay Kit (Abcam ab113849) according to the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2023Quote: ... The cytotoxicity of the different types of PBFSU NPs were evaluated using an MTS Assay Kit (Abcam, Cat# ab197010). INS-1 β-cells were cultured in a 96-well plate at 15000 cells/well for 24 h and subsequently treated with the respective doses of the different types of PBFSU NPs for 24 hours ...
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bioRxiv - Microbiology 2023Quote: ... Cell lines were validated by phenotypic screening and confirmed to be mycoplasma-free using a PCR detection kit (Abcam). All cells were maintained at 37 °C in a 95% humidity and 5% CO2 atmosphere.
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bioRxiv - Microbiology 2023Quote: ... The amount of histamine released into the culture supernatant was determined using a histamine detection kit (Abcam, Cambridge, MA) as described previously [19] ...
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bioRxiv - Microbiology 2023Quote: We used a Cellular ROS Assay Kit (Red) (ab186027) to measure reactive oxygen species (ROS) in our biofilm samples following the manufacturer’s protocol (Abcam). A 25-μL (OD600=0.1 ...
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bioRxiv - Cell Biology 2023Quote: ... Measurement of apoptosis and necrosis was carried out following the instructions from the Apoptosis/Necrosis Assay Kit from Abcam. Supernatant from the treated THP-1 macrophages was collected ...
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bioRxiv - Microbiology 2023Quote: ... The tilter of the sgRNA lentiviral library was determined by RT-qPCR using the qPCR Lentivirus Titer Kit (Abcam) following the protocol provided by the manufacturer ...
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bioRxiv - Microbiology 2024Quote: The amount of ATP generated in the gastrocnemius muscle samples was measured using the ATP assay kit (Abcam, 83355) described in the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2024Quote: ... The slides were further processed using rabbit specific HRP/DAB (ABC) detection IHC kit according to the manufacturer’s protocol (Abcam). Slides were subsequently scanned on an Akoya Vectra Polaris scanner scanning at ×20 magnification and semi-quantitative analysis of cytoplasmic FASN ...
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bioRxiv - Genomics 2024Quote: ... and ∼10 µg total protein was used to measure TET activity using the TET Hydroxylase Activity Quantification Kit (Abcam) following the manufacturer’s protocol.
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bioRxiv - Microbiology 2024Quote: ... cAMP levels in the cells were determined in a plate reader by using fluorescence-based cAMP detection kit (Abcam) according to the manufacturer’s recommendations and normalized to the total protein present in the samples as determined by a BCA protein estimation kit (Pierce) ...
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bioRxiv - Cell Biology 2024Quote: Islet were washed once with PBS and cholesterol labelled according to the Cholesterol Assay kit (cell-based) (ab133116, Abcam). Briefly ...
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bioRxiv - Bioengineering 2024Quote: ... Intracellular lipid accumulation of all viable clones was quantified with Oil Red O Stain Kit (ab150678; Abcam, Cambridge, UK) on day six (Supplementary Fig ...
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bioRxiv - Neuroscience 2024Quote: ... Tissue ferrous (Fe2+) and total iron content were determined using the colorimetric Iron Assay Kit (Abcam, Cat. No. ab83366) following the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2024Quote: ... SH-SY5Y cells under respective conditions were stained with cathepsin D and L kits (Abcam, Cat# ab65302 and ab270774) following company protocols ...
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bioRxiv - Molecular Biology 2024Quote: ... LDH levels were quantified in cell supernatants using an LDH-Cytotoxicity Assay Kit following manufacturers’ protocol (abcam, United Kingdom). To quantify caspase-3/7 activity ...
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bioRxiv - Physiology 2024Quote: Sphingomyelin levels in mAoECs after treatment with vehicle or wortmannin was determined by a sphingomyelin assay kit (abcam, ab138877) while following manufactures instructions.
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bioRxiv - Cancer Biology 2021Quote: ... Mitochondrial fractions (for cytochrome c measurement) and membrane fractions (for Myr-RSK3 measurement) were extracted from cells using a Cell Fractionation kit (Abcam) and a Mem-PER™ Plus Membrane Protein Extraction Kit (Thermofisher) ...
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bioRxiv - Cancer Biology 2021Quote: ... and resuspended in 1X Binding buffer with Annexin V and propidium iodide (PI) with a Annexin V-FITC Apoptosis Detection Kit (Abcam), according to the instructions of the manufacturer ...
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bioRxiv - Cancer Biology 2021Quote: ... adherent and floating cells were collected and labeled with the Annexin V-Cy3 Apoptosis Detection Kit (Abcam, Cambridge, UK, #ab14143) according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2021Quote: Fibrinogen was measured in P10 serum (6 Jag1+/+ and 6 Jag1Ndr/Ndr mice) using a commercial kit (cat. #ab213478, Abcam), according to manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2021Quote: ... to avoid bias by color changes in the medium and inhibition of growth respectively total cellular viability was assayed using the MTS Assay Kit (Abcam). 100% viability was defined as the readout from non-treated cells (addition of PBS ...
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bioRxiv - Molecular Biology 2021Quote: ... gel followed by either silver staining with Pierce Silver Stain Kit (Thermo) or Western blotting against anti-Nop58 (#ab236724, Abcam) and anti-GAPDH (#5174s ...
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bioRxiv - Cell Biology 2019Quote: ... oryzae conidia were harvested and normalized using the Iron Assay Buffer (100 ml) from the Iron Assay kit (Abcam, ab83366), to reach a final conidia count of 3×105/well ...
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bioRxiv - Biochemistry 2019Quote: Cathepsin D activity (used as a proxy for total lysosomal hydrolase activity) was measured by using Cathepsin D Activity Assay Kit (Abcam). Cells were collected ...
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bioRxiv - Cell Biology 2019Quote: Cells treated with 0.1% DMSO or 5 μM SGC-0946 were stained with Annexin V-FITC and DAPI following the protocol of the Annexin V-FITC Apoptosis Detection Kit (Abcam). Fluorescence was detected by FACS using the CyAn ADP Analyzer (Beckman Coulter ...
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bioRxiv - Cancer Biology 2019Quote: ... cells were lysed and the level of PKC-specific kinase activity was measured in 30 μg cell lysate using the PKC Kinase Activity Assay Kit (AbCam) as described by the manufacturer ...
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bioRxiv - Developmental Biology 2019Quote: ... 21 Gclc KO and Gclc WT mice were anesthetized and eyes enucleated and immediately assayed using the GSH/GSSG Ratio Detection Assay Kit (Fluorometric-Green) (Abcam) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2020Quote: ... The culture supernatants containing pseudotyped viruses particles were either used immediately or flash frozen in aliquots and stored at 80°C until use after being concentrated using a PEG virus precipitation kit (Abcam). Incorporation of the SARS-CoV-2 S protein into the SARS-CoV-2pp was confirmed using Western Blot analysis with anti-SARS-CoV-2 S1 chimeric monoclonal antibody with combined constant domains of the human IgG1 molecule and mouse variable regions (40150-D001 Sinobiological ...
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bioRxiv - Bioengineering 2021Quote: ... stained with 10 µl of AnnexinV-FITC and propidium iodide (PI) following manufacturer’s instructions (Annexin-V Apoptosis Staining Detection Kit, ab14084, Abcam, USA). Metabolic activity tests were performed using an alamarBlue cell viability assay (BUF012 ...
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bioRxiv - Biochemistry 2020Quote: ... and M2 activated (n = 3) was reported as fold-change from control using a commercial intracellular ROS fluorescence assay kit (Abcam, used according to manufacturer’s instructions) ...
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bioRxiv - Cancer Biology 2020Quote: The relative MDA concentration in cell or tumor lysates was assessed using a Lipid Peroxidation (MDA) Assay Kit (Abcam, #ab118970) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2020Quote: The amount of ATP released into the medium was measured with an ATP Assay Kit (Fluorometric, ab83355; Abcam, Cambridge, UK) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2021Quote: ... RNA (1 μg) was used to synthesize cDNA from each donor using the high-capacity reverse transcriptase cDNA synthesis kit (Abcam). DAT ...
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bioRxiv - Cell Biology 2021Quote: ... ovalbumin and CD4-Fc fusion proteins were labelled with allophycocyanin (APC) with the Lightning-Link Labelling Kit (Abcam, Cambridge, UK).
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bioRxiv - Microbiology 2021Quote: Culture media and cellular extracts were collected and centrifuged in preparation for LDH activity quantitation with a commercially available kit (#ab102526, Abcam). LDH activity was measured by a microplate reader (Tecan Infinite M200 Pro ...
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bioRxiv - Bioengineering 2020Quote: ... hCMEC/D3 and primary rodent microglia cells were labeled with Cell Tracking Dye Kit - Green - Cytopainter (ab138891, Abcam, Cambridge, UK) and Cell Tracking Dye Kit - Deep Red - Cytopainter (ab138894 ...
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bioRxiv - Immunology 2021Quote: ... the HIV Env 1086.c K160N gp120 protein was conjugated to biotin using the Fast Type A Biotin Conjugation kit (Abcam) and then captured on avidin-labeled fluorescent beads (NeutrAvidin™ ...
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bioRxiv - Cancer Biology 2020Quote: Cytosol and mitochondria were separated from A375 cells 2 hr or 4 hr after treatment using the Mitochondria/Cytosol Fractionation Kit (Abcam) following the manufacturer’s instructions.
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bioRxiv - Cancer Biology 2022Quote: ... cytotoxicity and killing potential was measured by LDH amount in the cell supernatant using the LDH-Cytotoxicity Assay Kit (Ab65393, Abcam) following the manufacturer’s protocol.
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bioRxiv - Systems Biology 2022Quote: ... Enzyme activity was assessed on the basis of glutamate production as measured with a glutamate assay kit (Abcam, Cat# 138883). The IC50 value was estimated from biological quadruplicates with a four-parameter logistic model63 and with the use of JMP pro 15 software (version 15.1.0).
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bioRxiv - Physiology 2022Quote: 10 µg of protein from 4-week-old adrenals were used for measurement with PKA kinase activity kit (ab139435, Abcam).
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bioRxiv - Cancer Biology 2022Quote: Cytotoxicity and apoptosis were assessed using the Annexin V-CF Blue 7-Aminoactinomycin D (7-AAD) Kit (Abcam Cat# AB214663) as per manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2022Quote: ... terminal deoxynucleotidyl transferase (TdT)-mediated dUTP-digoxigenin nick-end labeling (TUNEL) staining was performed using TUNEL assay kit (Abcam, ab206386) according to the manufacturer’s instructions.
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bioRxiv - Cancer Biology 2021Quote: Cell transformation assays in immortalized HBEC hTERT were performed using the Cell Transformation Assay Kit-Colorimetric (Abcam Cat No. ab235698). Based on manufacturer’s protocol ...