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Citations for abcam :
2601 - 2650 of 4295 citations for BCA Protein Colorimetric Detection Kit 2 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... The genomic DNA crossed with Foxo1a protein was immunoprecipitated by using 5 μg Anti-6×His tag antibody (abcam, ab213204). Antibody against lgG was used as a negative control ...
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bioRxiv - Synthetic Biology 2024Quote: ... Percent purity of each sample was determined by running diluted aliquots of each purified protein on a 4-12% Bis-Tris gel and staining with Optiblot Blue (Abcam). After destaining ...
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bioRxiv - Molecular Biology 2024Quote: ... anti-voltage-dependent anion-selective channel protein 1 (VDAC1)/porin (mouse monoclonal, #ab14734, RRID: AB_443084, Abcam; 1:2000 for WB), anti-α-tubulin (rabbit polyclonal ...
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bioRxiv - Evolutionary Biology 2024Quote: ... The co-immunoprecipitated and immunoprecipitated proteins were then characterized by Western Blotting using anti-HA monoclonal antibody (ab18181, Abcam, USA) and anti-myc monoclonal antibody (ab18185 ...
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bioRxiv - Immunology 2024Quote: ... Expression of gE protein on cell surface was determined by staining cells with anti-gE mAb (1:200 dilution, Abcam) for 1 hour prior to incubation with PE-anti-human IgG Fc (1:200 dilution ...
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bioRxiv - Genetics 2024Quote: ... Protein G beads (80 uL) and lysates were washed with RIPA buffer and immunoprecipitated with antibodies targeting H3K18ac (Abcam #ab40888), H3K18pr (PTM Bio #PTM-213) ...
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bioRxiv - Neuroscience 2023Quote: ... cells were stained for 10 minutes at 37°C with a chicken anti-green fluorescent protein (GFP) antibody (cross-reactive with YFP; ab13970, AbCam) diluted 1:1000 in neurobasal medium to stain for surface GluN2B ...
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bioRxiv - Cell Biology 2024Quote: ... Total 500 μg of cellular extract was precleared with protein A-agarose beads and was incubated with anti-vinculin (Abcam) antibody overnight at 4°C in a rotator ...
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bioRxiv - Biochemistry 2023Quote: ... The purity and size of the protein has been evaluated by Sodium Dodecyl Sulfate Polyacrylamide gel electrophoresis (SDS-PAGE) using RunBlue (#ab270467 Abcam) 4-12% Bis-Tris polyacrylamide gel ...
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bioRxiv - Neuroscience 2023Quote: ... PFC sections were incubated overnight at 4°C in a blocking solution containing primary antibodies against green fluorescent protein (GFP; 1:1000 dilution, raised in goat, AB6673, Abcam). VTA sections were incubated overnight in a blocking solution containing antibodies for green fluorescent protein (GFP ...
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bioRxiv - Neuroscience 2023Quote: ... VTA sections were incubated overnight in a blocking solution containing antibodies for green fluorescent protein (GFP; 1:1000 dilution, raised in goat, AB6673, Abcam) and tyrosine hydroxylase (TH ...
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bioRxiv - Pathology 2023Quote: ... Five micrograms of extracted protein was used for western blot analysis with primary antibody against MMP2 (1:200; ab92536, Abcam). The signal intensity of bands was quantified using the Image J densitometry software (NIH).
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bioRxiv - Pathology 2023Quote: ... The expression of the MMP2 proteins in cell lysate and culture medium were confirmed by western blot analyses using antibody against MMP2 (ab92536, Abcam).
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bioRxiv - Systems Biology 2023Quote: ... The protein expression and solubility were evaluated by both Coomassie stain and western blot (WB) of the GST and MBP fusion proteins using anti-GST (abcam) and anti-MBP (NEB ...
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bioRxiv - Cancer Biology 2023Quote: ... 20 µl of protein A magnetic beads pre adsorbed with 0.5 µg of anti-lamin A/C antibody (Abcam, #ab108595) were added to the protein mixture and incubated for 2 hours at RT ...
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bioRxiv - Neuroscience 2023Quote: Concentrations of plasma LPS-binding protein (LBP) were examined via Mouse LBP SimpleStep ELISA according to the manufacturer’s protocol (Abcam, #ab269542). Plasma samples were prepared at a 1:16,000 dilution ...
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bioRxiv - Genetics 2023Quote: ... and Atg18 knockdown flies with the expression of mCherry-Atg8a fusion protein were counterstained with diluted CytoPainter Phalloidin-iFlor 488 (from Abcam) for F- actin at room temperature for 15 minutes before the tissues were mounted in phosphate-buffered saline solution (PBS ...
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bioRxiv - Immunology 2022Quote: ... Proteins were transferred onto Hybond nitrocellulose membranes (Amersham biosciences) and probed with antibodies specific for alpha smooth muscle actin (Abcam), BACE1 (Sigma) ...
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bioRxiv - Cancer Biology 2023Quote: ... The respective concentration of each sample was calculated based on the standard curve generated by known protein concentrations and measured optical density (OD) at 450 nm following background subtraction according to manufacturer’s instructions (Abcam; ab277082).
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bioRxiv - Biochemistry 2023Quote: ... The tri-methylated lysine 9 and 27 analogs on H3K9C/C110A and H3K27C/C110A proteins were confirmed by western blot assays using monoclonal anti-Histone H3K9me3 and H3K27me3 antibody (Abcam).
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bioRxiv - Cell Biology 2024Quote: ... The gels were run in MOPS buffer at 200 V for 45 min and stained with Instant- Blue Coomassie protein stain (Abcam).
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bioRxiv - Molecular Biology 2024Quote: ... A total of 3 μg of the peptide was loaded on an SDS–polyacrylamide gel and stained with InstantBlue Coomassie Protein Stain (Abcam). Intensity of the remaining peptide bands were quantified using the ImageJ and plotted.
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bioRxiv - Neuroscience 2024Quote: ... 5mg per sample of lysate was added to 50ul Protein G beads (Cytiva 28951379) pre-bound with 10ul anti-PCB (Abcam 110314 ...
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bioRxiv - Molecular Biology 2024Quote: Protein-nucleosome binding assays were performed by incubating the abovementioned purified Pioneer-seq nucleosome library with human TP53α (Abcam ab84768), TAp63α (Origene TP710041) ...
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bioRxiv - Cell Biology 2024Quote: ... Lifted cells were washed 2x with cold dPBS (HyClone, SH30264.02) and incubated with lectins at 10 µg/mL or MBL2 protein (Abcam, ab151947) at 1:50 in 3% bovine serum albumin (BSA ...
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bioRxiv - Cancer Biology 2021Quote: ... Multi-color immunohistochemistry was conducted on a cohort of early-stage non-small cell lung cancer patients as described elsewhere (https://jitc.bmj.com/content/9/2/e001469) with polyclonal rabbit anti-Neutrophile Elastase antibody (Abcam, Cambridge ...
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bioRxiv - Cell Biology 2020Quote: ... Immunohistochemistry was executed on human colonic tissue using antibodies against Epac-2 and Rap-1 (1:100; Abcam).
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bioRxiv - Genetics 2021Quote: ... Lysates were precleared and then immunoprecipitated with 2 μl anti-histone H3 (monomethyl K9) antibody (Abcam no. ab9045), 2 μl anti-histone H3 (dimethyl K9 ...
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bioRxiv - Bioengineering 2019Quote: ... diluted to 1.25 μg/mL with 0.1% BSA in PBS-0.1% Tween 20 and a secondary antibody of 2 μL/mL Alexa Fluor 488-anti mouse in 0.1% BSA in PBS-0.1% Tween 20 (Abcam). Nuclei were stained with Hoescht 33342 ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... Purmorphamine (9-Cyclohexyl-N-[4-(4-morpholinyl)phenyl]-2-(1-naphthalenyloxy)-9H-purin-6-amine) was from Abcam. SAG1.3 (3-Chloro-N-[trans-4-(methylamino)cyclohexyl]-N-[[3-(4-pyridinyl)phenyl]methyl]benzo[b]thiophene-2-carboxamide ...
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bioRxiv - Genomics 2021Quote: ... and sheared DNA was added to antibodies coupled to washed magnetic beads (anti-FLI1 2 µg, ab15289, Abcam; anti-ERG 2 µg ...
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bioRxiv - Neuroscience 2021Quote: ... and incubated with primary antibodies diluted in PBS containing 2% NHS overnight (1:500 rabbit anti-Iba1, Abcam, ab178846 ...
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CAP1 and cofilin1 cooperate in neuronal actin dynamics, growth cone function and neuron connectivitybioRxiv - Neuroscience 2020Quote: ... 10% donkey serum and 0.5% NP40 in PBS and stained over night at 4°C with following primary antibodies in 2% BSA and 0.5% NP40 in PBS: rat anti-Ctip2 (1:1,000, Abcam), rabbit anti-Neurofilament200 (1:80 ...
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bioRxiv - Neuroscience 2020Quote: ... and either vehicle or 3-((2-Methyl-4-thiazolyl)ethynyl) pyridine (MTEP) hydrochloride (Abcam Biochemicals, Cambridge, MA, USA) were infused over 2 min ...
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bioRxiv - Genomics 2022Quote: ... The Rabbit IgG control antibodies for ChIP-qPCR experiments were ab172730 (abcam, GR3298823-2, 2ug per 80ug chromatin), and Normal IgG Rabbit 2729 (Cell Signaling ...
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bioRxiv - Genomics 2022Quote: ... and incubated overnight at 4°C in either anti-dsDNA (Abcam, 1:5,000 in 2% BSA in TBST) or anti-5-methyl-cytosine (5mC – Aviva Biosystem clone 33D3 ...
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bioRxiv - Microbiology 2021Quote: ... cells were stained with the primary rabbit polyclonal anti-SARS-CoV-2 spike glycoprotein antibody (1:1000, Abcam) for 1 h at RT or overnight at 4 °C ...
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bioRxiv - Developmental Biology 2022Quote: ... The blastocysts were incubated with primary antibodies for 8-Hydroxy-2-deoxyguanosine (8-OHdG, 1:200; ab48508, Abcam), 8-iso Prostaglandin F2 alpha (PGF2 alpha ...
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bioRxiv - Neuroscience 2019Quote: ... incubated 2 h at room temperature with secondary antibody (1/500 biotinylated goat anti-guinea pig ab6907 Abcam), washed again with PBS ...
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bioRxiv - Developmental Biology 2020Quote: ... Blocking was performed with 2% BSA in 0.5% Triton/PBS and rabbit polyclonal anti-mCherry primary antibody (Abcam) was used in 1:500 dilution overnight at 4°C ...
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bioRxiv - Molecular Biology 2019Quote: ... 2 μg of each sample was subjected to slot-blot for immunoblotting with anti-dsDNA antibody (Abcam, ab27156) to confirm equal DNA loading.
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bioRxiv - Neuroscience 2021Quote: ... Grids were incubated for 2 h in the presence of anti-LRRK2 (1:20 dilution, Abcam, cat. # ab133474) and anti-CD63 antibodies (1:40 dilution) ...
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bioRxiv - Immunology 2021Quote: Sonicated chromatin samples of 20,000 cells per assay were profiled for H3K27ac (abcam, cat: ab4729, lot: GR312651-2) with MOWChIP-seq as described in our previous publications53,58 ...
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bioRxiv - Cell Biology 2019Quote: ... The following antibodies were used for Western blot: rabbit anti-DYNLL1/2(1:3000, clone EP1660Y, Abcam, #ab51603), mouse anti-α7 (1:3000 ...
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bioRxiv - Immunology 2021Quote: ... the cells were marked with 4′,6-diamidino-2-phenylindole (DAPI) (cat. n° ab104139, Abcam, Cambridge, MA, USA), and incubated for 5 min ...
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bioRxiv - Neuroscience 2020Quote: ... mice received intraperitoneal injections (2 mg/kg) of each of the endothelin receptor antagonists BQ-123 (ab141005, Abcam) and BQ-788 (ab144504 ...
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bioRxiv - Cancer Biology 2021Quote: ... USA) and oligodendrocyte lineage transcription factor 2 (OLIG2; clone EPR2673, rabbit monoclonal, dilution 1:50, Abcam, Cambridge, UK).
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bioRxiv - Cancer Biology 2021Quote: ... Cells were then incubated with goat F(ab’)2 anti-Human IgG Fc-FITC antibody (Abcam, Cambridge, MA) for 30 minutes in the dark on ice ...
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bioRxiv - Neuroscience 2021Quote: ... and then blocking solution (PBS supplemented with 2% Bovine Serum Albumin (Roth) and 5% Normal Goat Serum (Abcam)) was applied for 1 hour at room temperature ...
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bioRxiv - Cell Biology 2021Quote: ... for 2 h at 4°C followed by incubation with primary antibody to TCFL5 (1:50; 188075, Abcam) or control IgG (normal rabbit IgG ...