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Citations for abcam :
2401 - 2450 of 4295 citations for BCA Protein Colorimetric Detection Kit 2 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: ... protein expression was determined by Western blotting using as primary antibodies anti-FLAG or anti-V5 tag antibody (AbCam; 1:500 ...
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bioRxiv - Biophysics 2022Quote: ... The following primary antibodies were used to detect the presence of indicated proteins in this study: SETD3 (ab176582; 1:5,000; Abcam), Actin (A2066 ...
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bioRxiv - Biochemistry 2021Quote: ... Proteins were analyzed by Western Blot using anti-UBF and anti-Top2a antibodies (sc-9131, Santa Cruz; ab12318, Abcam).
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bioRxiv - Neuroscience 2021Quote: 16 μg of protein extracted from frozen human tissue samples was employed in a hemoglobin sandwich ELISA (Abcam Ab157707). The same lot of this kit was employed for the analysis of all samples and the assay was performed according to the manufacturer’s instructions.
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Physiological activation of the nephron central command drives endogenous kidney tissue regenerationbioRxiv - Physiology 2021Quote: ... Primary antibodies and dilutions were as follows: anti-Wilms tumor protein antibody (WT1, 1:100, AB89901, Abcam, Cambridge, UK), anti-Meis2 antibody (1:100 ...
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bioRxiv - Neuroscience 2020Quote: ... proteins (0.8-1 mg) were rotated overnight at 4°C with 5 µg of mouse anti-SGTa antibody (Abcam) or control mouse IgG and protein G Dynabeads (Invitrogen) ...
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bioRxiv - Physiology 2022Quote: ... rat anti-CD45 (Becton Dickinson 550539, 1:200) and rat anti myelin basic protein (MBP, Abcam ab7349, 1:200). The following donkey-raised secondary antibodies (all in 1:400 dilution unless otherwise stated ...
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bioRxiv - Systems Biology 2020Quote: ... The primary antibodies against the following proteins were used in this study: ATP synthase complex (Abcam, Cambridge, MA, USA), KIF5B (Abcam ...
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bioRxiv - Molecular Biology 2020Quote: ... The resulting nuclear extract was incubated with Dynal protein G beads which were preincubated with 5μg of H3K27me3 or H3 antibody (ab6002 and ab1791, Abcam) respectively at 4°C overnight ...
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bioRxiv - Bioengineering 2019Quote: ... Primary antibodies were used as follows: rat anti-glial fibrillary acidic protein (GFAP, 1:100, Abcam, Cambridge, MA, USA) for astrocytes and rat anti-CD11b (1:100 ...
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bioRxiv - Immunology 2021Quote: ... and the obtained values represent the ratio between the immunodetected protein and the glyceraldehyde 3-phosphate dehydrogenase (GAPDH, Abcam) loading control ...
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bioRxiv - Cancer Biology 2021Quote: ... Proteins were then incubated with a 10-fold molar excess of the chosen guanine nucleotide (GMPPNP or GDP (AbCam)) for 3 hours at room temperature (22 °C ...
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bioRxiv - Cell Biology 2021Quote: ... as determined by the staining of the SDS-PAGE gels with CBBR (InstantBlue Coomassie Protein Stain [ISB1L], Abcam: ab119211). Aliquots (10 μl ...
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bioRxiv - Neuroscience 2022Quote: ... Samples were washed twice with PBS containing 0.025% triton-x for 5 minutes and then blocked with protein block (ab64226, Abcam) for 20 minutes ...
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bioRxiv - Neuroscience 2022Quote: Immunofluorescence was used to visualize co-expression of certain proteins in glial or neuronal cell types: C3(Abcam 181147) and S100β (Abcam 41428) ...
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bioRxiv - Molecular Biology 2022Quote: ... HA-tagged proteins and histone H3 were detected with anti-HA (Abcam ab9110, 1:1,000 in 1% milk-PBST) and anti-H3 (Abcam ab1791 ...
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bioRxiv - Neuroscience 2024Quote: ... and then incubated in primary antibodies against the first protein of interest and tubulin (Abcam, Cambridge, UK,; 1:5000) overnight at 4°C ...
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bioRxiv - Immunology 2024Quote: ... IHC staining was applied to examine the expression of the protein encoded by Mpo (Myeloperoxidase, 1:1000, ab208670, Abcam) and Il1b (IL-1 beta ...
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bioRxiv - Bioengineering 2023Quote: ... The gels were washed once with distilled water and stained with InstantBlue® Coomassie Protein Stain (Abcam, Cambridge, UK). The gels were viewed using a ChemiDOC XRS molecular imager (Biorad ...
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bioRxiv - Cell Biology 2023Quote: ... Proteins were probed in Can Get Signal Solution1 (Toyobo) using following primary antibodies: anti-Gpx4 (1:10,000, Abcam, ab125066), anti-β-actin (1:20,000 ...
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bioRxiv - Genomics 2024Quote: ... 500 mM NaCl and 0.2% Tween 20) and precleared with 100 μL 50% protein G Sepharose bead (Abcam, ab193259) slurry for 20 min at 4°C ...
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bioRxiv - Molecular Biology 2023Quote: ... In each capillary 0.2mg/mL protein was loaded for analysis with antibodies to dystrophin (Abcam # ab15277, dilution 1:15) as in 26 ...
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bioRxiv - Microbiology 2023Quote: ... or mouse antibodies recognizing cellular proteins (anti-EEA1, BD Biosciences, 610457, 1:100; anti-VPS35, Abcam, ab57632, 1:200); rabbit antibodies recognizing cellular proteins (anti-TGN46 ...
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bioRxiv - Immunology 2023Quote: ... Obtained protein fraction has been subjected to Sodium Dodecyl Sulfate Polyacrylamide gel electrophoresis (SDS-PAGE) using RunBlue (#ab270467 Abcam) 4-12% Bis-Tris polyacrylamide gel ...
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bioRxiv - Genomics 2023Quote: ... 500 mM NaCl and 0.2% Tween 20) and precleared with 100 μL 50% protein G Sepharose bead (Abcam, ab193259) slurry for 20 min at 4°C ...
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bioRxiv - Biochemistry 2022Quote: ... and the quality of the purification was assessed via SDS-PAGE followed by InstantBlue Coomassie protein stain (abcam: ab119211) staining and immunoblotting ...
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bioRxiv - Biochemistry 2023Quote: ... Then gels were either stained with Coomassie-blue (15 µg of protein per lane) (Instant blue, abcam, Cambridge, UK) or transferred to nitrocellulose membranes (0.25 µg or 1 µg of protein per lane for the monomeric transporters and heterodimer respectively ...
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bioRxiv - Biochemistry 2023Quote: ... The protein was transferred overnight to 0.22µ PVDF membranes and probed using either anti-HA (Abcam Cat# ab9110, RRID:AB_307019) or anti-Myc (Abcam Cat# ab32 ...
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bioRxiv - Neuroscience 2023Quote: ... Sections were incubated overnight in primary antibodies against green- fluorescent protein (GFP, 1:1000 in antibody diluent, Abcam, ab13970), calcitonin gene-related peptide (CGRP ...
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bioRxiv - Neuroscience 2023Quote: ... Blockade of IL-1R was performed using the recombinant mouse IL1-RA protein (1-1000 mg/mL, abcam #ab283475).
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bioRxiv - Biochemistry 2024Quote: Protein was visualized by SDS-PAGE analysis using 4-12% bis-tris mPAGE gels and stained using InstantBlue (Abcam), according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2024Quote: ... Membranes containing GFP-tagged proteins were probed with a 1:1000 of goat anti-GFP polyclonal antibody (Abcam ab5450) and a 1:5000 dilution of donkey anti-goat IRDye800CW (Licor 926-32214) ...
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bioRxiv - Neuroscience 2024Quote: ... Samples were normalized to either actin or GAPDH housekeeping proteins with monoclonal antibodies (anti-actin; 1∶1000, Sigma or anti-GAPDH; 1:10,000 Abcam).
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bioRxiv - Cancer Biology 2020Quote: ... Membranes were blocked in 20% milk in PBST for 1h following primary antibody incubation (COX-2: Abcam, Cat# ab15191 ...
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bioRxiv - Genetics 2021Quote: ... the following antibodies were used: rabbit anti-Ki67 (1:200; 2 hours at room temperature; Abcam, Ab16667), rabbit anti-β-catenin (1:300 ...
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bioRxiv - Genomics 2021Quote: ... We used the following antibodies in this work: anti-Pol II-total (ab817, lot GR3271868-2, Abcam), anti-Pol II-S5 (ab5131 ...
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bioRxiv - Neuroscience 2020Quote: ... The efficiency of knockdown was assessed by immunostaining using the LRRK2 antibody [MJFF2 (C41-2)] (# ab133474, Abcam) at1:200 dilution ...
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The E3 ubiquitin-protein ligase MDM2 is a novel interactor of the von Hippel-Lindau tumor suppressorbioRxiv - Biochemistry 2020Quote: ... In this case 400 μgr of PNS were incubated with 2 μg of anti-GFP (Abcam, ab1218). Following Western blot ...
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bioRxiv - Molecular Biology 2021Quote: ... and MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) reagent (5 mg/mL MTT (Abcam ab146345) in PBS ...
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bioRxiv - Microbiology 2020Quote: 2 µl of EV isolates and two fold serial dilutions were spotted onto a nitrocellulose membrane (Abcam) and process for dot blot using anti-EV polyclonal murine serum (8 ...
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bioRxiv - Developmental Biology 2020Quote: ... The following antibody dilutions were used: rabbit polyclonal anti-Aconitase 2 antibody at 1:1000 (ab83528; Abcam), rabbit polyclonal anti-6X His tag antibody at 1:1000 (ab1187 ...
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bioRxiv - Neuroscience 2022Quote: ... C-175) and 3-((2-methyl-1,3-thiazol-4-yl)ethynyl)pyridine hydrochloride (MTEP hydrochloride, Abcam, ab120035) were prepared in distilled water and diluted with saline to the required concentration.
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bioRxiv - Immunology 2022Quote: ... incubated with 0.2 μg/mL of anti-SARS-CoV-2 spike glycoprotein antibody (abcam, Catalog No. ab272504) for 1 hour ...
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bioRxiv - Bioengineering 2022Quote: ... Then the organoids were incubated for 2 hours at RT with primary antibodies against cytokeratin 8 (Abcam, Cambridge ...
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bioRxiv - Pathology 2020Quote: ... Primary antibodies were incubated in blocking buffer for 2 h at room temperature (rabbit anti-ACE2 [Abcam #ab15348 ...
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bioRxiv - Genetics 2020Quote: ... 2 μg of purified mRNAs were mixed with 5 μg of anti-m A antibody (Abcam, ab151230) in 450 μL immunoprecipitation buffer (50 mM Tris ...
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bioRxiv - Neuroscience 2020Quote: ... membranes were incubated in 2% milk with the corresponding secondary horseradish peroxidase-conjugated antibodies (1:10.000, Abcam) for 1 hour at room temperature ...
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bioRxiv - Physiology 2021Quote: ... washed 2 × 5 min in PBS and then incubated with primary antibodies against Laminin-α2 (#11576, Abcam) in antibody solution containing 10% goat serum in PBS ...
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bioRxiv - Developmental Biology 2021Quote: ... incubated with α-mouse IgG-AP conjugated (1:2000 in 2% BSA/PBSTx) (ab5880 Abcam, Cambridge, UK) at 4°C overnight ...
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bioRxiv - Animal Behavior and Cognition 2021Quote: ... and fragmented chromatin was processed for ChIP with 2 ug histone H3K27Ac antibody per sample (Abcam ab4729), using one million nuclei for each IP ...