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Citations for abcam :
2701 - 2750 of 4295 citations for BCA Protein Colorimetric Detection Kit 2 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2022Quote: ... Sections were washed at least three times with PBST and incubated with native Streptavidin protein DyLight 488 (1:750 dilution; Abcam, ab134349) in 0.5% fish skin gelatin with PBST for 2 hours ...
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bioRxiv - Physiology 2022Quote: ... 50-100 μg of protein were used for the pull down of FLAG-tagged Kcnq4 proteins with monoclonal anti-FLAG M2 antibody produced in mouse (mouse monoclonal Anti-FLAG M2 antibody, F3165) and Protein G Sepharose beads (Abcam, UK) at 4 °C overnight ...
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bioRxiv - Evolutionary Biology 2024Quote: ... the included Pierce™ Protein A/G Plus agarose beads were incubated with rabbit anti-myc polyclonal antibody (ab9106, Abcam, USA) and cross-linked using 2.5 mM DSS crosslinker ...
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bioRxiv - Neuroscience 2022Quote: ... NF200 (1:500, Chemicon International, Temecula, CA, USA) rabbit monoclonal amyloid precursor protein (APP) antibody [Y188] (1:500, Abcam, Cambridge, MA) and NeuN (1:500 ...
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bioRxiv - Genomics 2022Quote: ... The ChIP assay was performed with Dynabead Protein G beads and histone antibodies (H3K4me3: Millipore #07-473, H3K4me1: Millipore #07-436, H3K27me3: Millipore #07-449, H4K27ac: abcam ab4729). After binding to antibodies ...
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bioRxiv - Microbiology 2023Quote: ... Lysates were incubated with antibodies for one hour at 4°C in an overhead tumbler before immune complexes were retrieved by protein G (GE Healthcare or abcam) or A Sepharose (GE Healthcare) ...
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bioRxiv - Molecular Biology 2023Quote: ... The supernatant was transferred to a fresh Eppendorf tube and the protein concentration was assessed using Bradford reagent (Abcam, cat#ab119216) and stored at -80°C when not used immediately ...
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bioRxiv - Neuroscience 2023Quote: ... After SDS-PAGE and proteins transferred on PVDF membranes were incubated overnight at 4°C with Total OXPHOS Human WB Antibody Cocktail (Abcam, UK) and subsequently detected with HRP-conjugated goat anti-rabbit IgG secondary antibody (1:4000 ...
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bioRxiv - Molecular Biology 2023Quote: ... were coupled with 10μg of anti-protein of interest (POI; anti-Foxg1 ChIP-grade, rabbit polyclonal, Abcam #ab18259; anti-GFP, rabbit polyclonal, Abcam #ab290), or 10μg of rabbit IgG (Millipore #12370 ...
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bioRxiv - Physiology 2023Quote: ... Frozen sections were fixed with fresh 4% paraformaldehyde and neutrophil elastase protein was detected using anti-neutrophil elastase antibody (Abcam #68672).
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bioRxiv - Molecular Biology 2023Quote: ... which was performed overnight at 4°C by incubating the shared DNA-protein complex with 10 µg of anti-ADNP (Abcam Ab231950) antibody ...
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bioRxiv - Developmental Biology 2023Quote: ... nucleoplasm and chromatin fractions were used for protein analysis with western blotting with the following antibodies were used: anti-DHX9 (Abcam 26271), anti-TBP (Abcam 818) ...
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bioRxiv - Biochemistry 2023Quote: ... Denatured samples were resolved in 4-12% Bis-Tris SDS-PAGE gradient gels and stained with InstantBlue® Coomassie protein stain (Cat#ab119211, abcam). Protein bands were excised from gel ...
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bioRxiv - Cancer Biology 2022Quote: Protein array analysis was performed using the Human MAPK Phosphorylation Antibody Array (ab211061) according to the manufacturer’s instructions (Abcam, Boston, MA). Visualization was performed with the ChemiDoc Touch Imaging System (Bio-Rad ...
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bioRxiv - Genomics 2023Quote: ... The heterozygous expression of the Dam-Lamin B1 fusion protein was further confirmed by Western blot using an anti-HA antibody at 1 in 5000 dilution (Abcam, ab9110) and an anti-LaminB1 antibody at 1 in 5000 dilution (Abcam ...
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bioRxiv - Cancer Biology 2023Quote: ... E0771 breast cells were treated with 0 and 100 ng/ml of active mouse recombinant Ifng protein (Abcam, catalog number: 259378) for 24 ...
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bioRxiv - Microbiology 2023Quote: ... Cells were blocked with Dako protein block then stained for cell surface Env with 2G12 anti-gp120 directly conjugated to APC (Abcam, ab201807) and diluted 1:100 in Dako antibody diluent (10ug/mL final concentration ...
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bioRxiv - Pathology 2023Quote: ... CEBP/α (diluted 1:400, Huabio, EM1710-20, CA) and fatty acid-binding protein (FABP4, diluted 1:200, abcam, ab92501, UK) overnight at 4□ ...
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bioRxiv - Biochemistry 2023Quote: ... Fractions were analyzed by separation using sodium dodecyl sulfate polyacrylamide gel electrophoresis (4–12% polyacrylamide) and staining with InstantBlue Coomassie Protein Stain (Abcam, UK). Fractions containing chalcone synthase were combined and loaded onto a HiLoad 16/600 Superdex 200 pg column ...
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bioRxiv - Neuroscience 2023Quote: ... CTIP2 (chicken ovalbumin upstream promoter transcription factor-interacting protein) antibody was added (rat, 1:500, Abcam, Cat: ab18465, Lot: GR3272266-13) to facilitate delineation of striatum and incubation continued until the 4 hours were completed ...
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bioRxiv - Biochemistry 2023Quote: ... A small aliquot of the collected fractions was loaded on to an SDS–PAGE gel and stained with InstantBlue Coomassie protein stain (abcam #ab119211) according to the manufacturer’s instruction ...
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bioRxiv - Cancer Biology 2023Quote: ... The fragmented chromatin fragments were pre-cleared and then immunoprecipitated with Protein A + G Magnetic beads coupled with anti-H3K36me2(Abcam, ab9049) or anti-p65 (Cell Signaling Technology ...
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bioRxiv - Molecular Biology 2024Quote: ... previously blotted membranes from Figure S2C,E were re-probed with 1:5000 mouse monoclonal anti-TATA binding protein antibody (Abcam #ab51841). The membrane in Figure S2H was probed for 1 h with 1:1000 rabbit anti-LARP7 antibody (Fortis/Bethyl #A303-723A ...
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bioRxiv - Microbiology 2024Quote: ... and a rabbit antibody recognizing cellular proteins or epitope tags (anti-EEA1, Cell Signaling Technology, 2411, 1:75; anti-TGN46, Abcam, ab50595 ...
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bioRxiv - Cell Biology 2020Quote: ... FVII and FX antigen (FVIIAg and FXAg) were measured in the cell medium using FVII ELISA kit and FX ELISA kit (Abcam, cat# ab168545 and ab108832) respectively ...
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bioRxiv - Pathology 2022Quote: ... membranes were incubated for 2 hrs with a secondary Donkey Anti-Rabbit IgG H&L (Abcam; ab16284, 1:10000) or Donkey Anti-Mouse IgG H&L (Abcam ...
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Glyoxal Does Not Preserve Cellular Proteins as Accurately as PFA: A Microscopical Survey of EpitopesbioRxiv - Cell Biology 2019Quote: ... lamin A/C (1:100 in PBS; following blocking with NGS, 2 hr at 37°C) (Abcam; ab108595, USA), or nucleostemin (1:250 in PBS ...
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bioRxiv - Neuroscience 2019Quote: ... Antibodies used were α-syn aggregate-specific antibody MJF14 (rabbit monoclonal MJFR-14-6-4-2. Abcam,1:25,000), the two phospho-serine129-specific antibodies ...
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bioRxiv - Immunology 2019Quote: ... then incubated in secondary antibodies in 0.1% Triton X-100/PBS for 2 hr at 4°C (goat anti-rat Alexa 488, Abcam, ab150157 at 1:500 ...
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bioRxiv - Genetics 2019Quote: ... Dynabeads M-280 sheep anti-rabbit IgG were incubated in lysis buffer and 0.5% BSA for 2 hrs with either rabbit anti-GFP (Abcam) for query IPs or 5 μl of rabbit-anti HA (Abcam ...
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bioRxiv - Cell Biology 2019Quote: ... the samples were incubated with Alexa Flour 488-conjugated goat anti-rabbit antibody (abcam, 1:500 in 2% BSA) and DAPI (Invitrogen ...
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bioRxiv - Bioengineering 2021Quote: ... Cells were resuspended at 1 × 106 cells/mL in cell-culture media supplemented with 2 mM thymidine (Abcam ab143719) and incubated at 37 °C in 5% CO2 for 18 hours ...
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bioRxiv - Neuroscience 2021Quote: Slices were placed into an open bath chamber and perfused constantly with aCSF (2-3 mL/min) supplemented with tetrodotoxin (0.5 μM; Abcam) and picrotoxin (50 μM ...
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bioRxiv - Cell Biology 2021Quote: ... Primary antibodies were used at 1:300 dilution in PBS with 0.1% Triton and 2% BSA (PBT) overnight at 4°C (Chicken anti-Bassoon Synaptic systems cat#141016, RRID:AB_2661779; Rabbit anti-Homer abcam cat#97593 ...
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bioRxiv - Physiology 2020Quote: ... rinsed in PBS 2×10min and then incubated in blocking solution containing 5% normal goat serum (NGS, Ab7481, Abcam), 0.5% Triton (T8787 ...
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bioRxiv - Neuroscience 2020Quote: Floating sections were blocked with 10% goat serum and incubated with chicken anti-MAP-2 (1:250, Abcam, USA) and rabbit anti- Cav-1 (1:1000 ...
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bioRxiv - Immunology 2021Quote: ... Proliferation of cells was measured staining of control and Irradiated UVC with either 2 μM Cell Trace Yellow (Abcam) according to kit protocol and analyzing the dilution of the dye at 24-hour periods over 3-days and measuring fluorescence intensity ...
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bioRxiv - Cell Biology 2020Quote: ... Blocking buffer was aspirated and cells incubated for 1 h at RT with a 1:1,000 dilution of primary antibodies in 2% BSA blocking buffer against dsDNA (Abcam, Cambridge ...
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bioRxiv - Cell Biology 2021Quote: ... anti-Acta2 (1:100) and anti-nuclear transcription factor Gli family zinc finger (Gli)2 (1:100; ab26056, Abcam) overnight at 4°C ...
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bioRxiv - Neuroscience 2022Quote: ... blocked with 5% BSA and 0.2% triton X-100 for 2 h and incubated with primary antibody (rabbit anti-pMLKL, 1:200 Abcam) overnight at 4 °C ...
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bioRxiv - Cell Biology 2022Quote: ... 10mg replicate 2) were incubated for 4 h at 4°C with 5 µg anti-GFP antibody (Abcam ab290) or mock-treated as a control ...
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bioRxiv - Neuroscience 2020Quote: Cell viability was measured after 3 days and 5 days of culture using an MTT [3-(4,5-dimethyldiazol-2-yl)-2,5-diphenyl tetrazolium bromide] assay (Abcam, ab211091) as per manufacturer’s protocol ...
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bioRxiv - Neuroscience 2020Quote: ... Sections were incubated for 2 hours with secondary antibody Alexa Fluor 488 donkey anti-rabbit (1:1000 abcam ab150073), and glial fibrillary acidic protein (GFAP ...
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bioRxiv - Neuroscience 2021Quote: ... CGP 55845 hydrochloride (CGP, 4 μM), 8-Cyclopentyl-1,3-dimethylxanthine (CPT, 10 μM), and dipropylcyclopentylxanthine (DPCPX, 2 μM) were obtained from Abcam. Ethanol (EtOH ...
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bioRxiv - Cell Biology 2019Quote: ... E-Cadherin (Cdh1) IHC staining was done by using mouse monoclonal [HECD-2] to E-Cadherin (Abcam, cat. ab1416) with M.O.M ...
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bioRxiv - Cancer Biology 2021Quote: ... Cells were fixed after 2 hour with 3.5% PFA and proceeded with IF staining with anti-BrdU antibody (ab6326, Abcam) followed with the secondary antibody conjugated with Alexa Fluor 594.
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bioRxiv - Neuroscience 2019Quote: ... The 2 ml of whole blood were added to 40 ml of red blood cell lysis buffer (Abcam, ab204733) and incubated for 10 min at room temperature to lyse red blood cells ...
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bioRxiv - Biochemistry 2021Quote: ... primary antibodies (OAT, Invitrogen #PA5-66715, 1:500; Fumarase, Invitrogen #PA5-82899, 1:500; Casein Kinase 2 beta, Abcam #ab151784 ...
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bioRxiv - Cell Biology 2021Quote: ... A monoclonal antibody that detects serine or threonine linked O-Linked N-Acetylglucosamine-RL-2 (ab2739) was from abcam. Anti-OGT (ab177941) ...
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bioRxiv - Microbiology 2021Quote: ... Cells were permeabilized with either 0.2% saponin or 0.2% triton (see Supporting Table 2) in PBS for 15 m and then blocked with 10% goat serum (Abcam) in PBS for 30 minutes (adding 0.2% saponin for saponin-permeabilized samples) ...