Labshake search
Citations for abcam :
2551 - 2600 of 2903 citations for 6H Thieno 2 3 b pyrrole 5 carboxylicacid ethyl ester since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Pathology 2021Quote: ... The samples were then washed for 5 minutes three times with TBT and incubated with the secondary antibody (Thermofisher A-21121 and Abcam® ab97239) for an hour at a dilution of 1:200 ...
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bioRxiv - Molecular Biology 2022Quote: ... Protein was adjusted to 400 µg and incubated O/N at 4℃ with 5 µg of IP-antibody (Table S1) or rabbit IgG control (Abcam, Cambridge, UK) in a final volume of 500 µl of NT2 buffer (150 mM NaCl ...
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bioRxiv - Neuroscience 2022Quote: Sections were briefly dried then washed 3x in 0.1% Triton X-100 in phosphate buffered saline (PBST) before they were blocked with 5% neutral goat serum (Abcam or Vector Labs). Sections were then incubated for double-labeling experiments with primary antibody (AB ...
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bioRxiv - Systems Biology 2022Quote: ... Cells were tehen incubated with a primary antibody solution containing BrdU antibody diluted 1:400 or 1:1000 in PBS containing 5% goat serum (Abcam, catalog # ab7481) and 0.1% Triton-X for 1 hour at room temperature ...
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bioRxiv - Molecular Biology 2019Quote: ... the membrane was first blocked in PBS 1X 0,02% Tween-20 containing 5% Milk and incubated overnight with the dsDNA-specific antibody (ab27156 Abcam, dilution 1/1000). To quantify G4 formation ...
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bioRxiv - Immunology 2021Quote: ... iPS cells were treated for 1 h with HDR enhancer (5 μM; IDT, Coralville, USA) and 10 μM Y-27632 (Abcam, Cambridge, UK). Electroporated cells were seeded on Laminin 521 (Biolamina ...
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bioRxiv - Neuroscience 2020Quote: ... Cells were incubated in one of the following primary antibodies in blocking solution containing 5% normal serum at 4°C overnight: F-actin (1:1000; Abcam, Cambridge, UK), mTOR (1:1000 ...
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bioRxiv - Immunology 2021Quote: ... Concentration of circulating LPS-binding protein (LBP) was determined in plasma samples (diluted 1:5) using Mouse LBP ELISA Kit (Abcam, Cambridge, MA) according to manufacturer’s instructions ...
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bioRxiv - Biophysics 2022Quote: ... The membrane was then treated with a blocking solution for half an hour (5% skim milk in 1% TBST) and incubated with the primary antibody (ab138501, Abcam, Cambridge, UK) at a ratio of 1:5,000 overnight at 4°C under constant motion ...
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bioRxiv - Neuroscience 2022Quote: ... membranes were washed three times with TBST for 5-min and then incubated for 1-hr with horseradish peroxidase-conjugated goat anti-rabbit IgG (ab6721, Abcam, Cambridge, MA). Membranes were washed three more times with TBST for 5-min.
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bioRxiv - Neuroscience 2024Quote: ... membranes were washed three times with TBST for 5-min and then incubated for 1-hr with horseradish peroxidase-conjugated goat anti-rabbit IgG (ab6721, Abcam, Cambridge, MA). Membranes were washed three more times with TBST for 5-min.
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bioRxiv - Cell Biology 2024Quote: ... Cells were then washed three times for 5 minutes each with PBS and then stained with a 1:3,000 dilution of Phalloidin-555 Alexafluor (# ab176756, Abcam, Cambridge, United Kingdom) in 300 µl PBS for 30 minutes ...
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bioRxiv - Bioengineering 2023Quote: ... which was prepared by 5% milk in TBS-T containing 1:10,000 horseradish peroxidase (HRP)-conjugated goat anti-mouse secondary antibodies (Abcam ab6789-1 MG). The secondary antibody incubation was applied for 1 hour at room temperature ...
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bioRxiv - Biochemistry 2023Quote: ... Stripped membranes were blocked in 5% NFDM in TBST (1 h, RT) before being probed with anti-Act1 (Abcam ab8224, 1:5000) loading control antibody (1 h ...
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bioRxiv - Cancer Biology 2023Quote: ... The membrane was blocked with 5% skim milk for 1 hour at RT before immunoblotting with antibodies against ACC1 (#ab72046, Abcam, 1:2000), ACC2 (#HPA006554 ...
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bioRxiv - Plant Biology 2023Quote: Immunoblotting was performed with antibodies diluted in the blocking solution (5% nonfat milk in TBS with 0.1% [v/v] Tween) at the following dilutions: α-GFP antibody (ab290, Abcam, Cambridge, UK), 1:8,000 ...
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bioRxiv - Genetics 2023Quote: ... was washed four times with 1X DPBS supplemented with 5 mg/mL BSA and subsequently crosslinked to 5 μg antibody (Anti-FOXA1 antibody: GeneTex, GTX100308; Anti-Fra2 antibody: Cell Signaling Technology, 19967S; Anti-H3K9me3 antibody: abcam, ab8898 ...
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bioRxiv - Molecular Biology 2023Quote: A pair of parotid salivary glands were harvested from each mouse (n=5/group) and directly placed in 500 uL of Lactate Assay Buffer from the L-Lactate Assay Kit (ab65331, Abcam, Cambridge, UK), 10 uL of protease inhibitor (PI ...
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bioRxiv - Pathology 2024Quote: ... was performed on 5 μm sections according to the previously reported protocol (Sellmer Ramos et al., 2023; ab108422; 1:500 dilution; Abcam; Waltham, MA) to identify adenomyotic foci in uterine cross-sections ...
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bioRxiv - Cell Biology 2020Quote: ... and labelled with primary antibody (1:1000 diluted in blocking buffer made up by 1% non-fat dry milk in calcium and magnesium free PBS) to tyrosinated tubulin [YL1/2] (rat monoclonal, ab6160, Abcam) for 45 min at room temperature which is followed by PBS washes repeated three times and 10 min of blocking ...
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bioRxiv - Cell Biology 2020Quote: ... Slides were washed 3 times in PBS and then incubated for 2 h in secondary antibody (1:200 dilution AlexaFluor647 conjugated donkey anti-goat (ab150135, Abcam) and 1:200 dilution FITC-conjugated rabbit anti-rat (ab6730 ...
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bioRxiv - Cancer Biology 2020Quote: ... Membranes were blocked in 20% milk in PBST for 1h following primary antibody incubation (COX-2: Abcam, Cat# ab15191, 1:1000 dilution, 1h at room temperature in 1% BSA; CYP2E1: Abcam, Cat# ab28146 ...
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bioRxiv - Systems Biology 2022Quote: ... and the supernatant containing chromatin was incubated with 20 μg of antibody (INO80 antibodies, abcam cat # ab105451, lot GR3206182-2) overnight at 4°C ...
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bioRxiv - Animal Behavior and Cognition 2022Quote: ... and 2% NGS (block) for 1h at room temperature before incubation in primary antibodies (rabbit anti-Synapsin I, ab64581, Abcam; guinea pig anti-GLT-1 ...
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bioRxiv - Neuroscience 2021Quote: ... Then the cells were blocked in 10% donkey serum for 2 h and incubated with primary antibodies (goat anti-Iba1, 1:400, Abcam; mouse anti-CD68 ...
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bioRxiv - Neuroscience 2021Quote: ... sections were incubated for 48 hours at 4°C on a shaker in primary antibody solution with 2% NHS (1:2000 Rabbit anti-C-FOS, ab208942, Abcam; 1:1000 Rat anti-Ctip2 ...
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bioRxiv - Neuroscience 2021Quote: ... sections were incubated for 48 hours at 4°C on a shaker in primary antibody solution with 2% NHS (1:2000 Rabbit anti-C-FOS, ab208942, Abcam; 1:1000 Rat anti-Ctip2, ab18465, Abcam; 1:1000 Rabbit anti-GABAalpha6 ...
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bioRxiv - Immunology 2020Quote: ... 6 µg of pre-cleared chromatin was immunoprecipitated with 2 µg/IP anti-Histone H3K27Ac (Abcam Cat#ab4729; RRID: AB_2118291), 4 µg/IP of anti-LXRα/β (provided by Knut Steffenssen ...
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bioRxiv - Molecular Biology 2021Quote: ... blocked in 2% normal donkey serum/1% BSA in PBS and incubated overnight with guinea pig anti-insulin (1:500, Abcam) at 4°C ...
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bioRxiv - Cell Biology 2022Quote: ... and incubated with primary antibodies against Rad53 (1:4000; rabbit, ab104332, Cell Signalling) or Tubulin (1:2000; rat, YL1/2, Abcam) diluted in TBS-T containing 1% BSA for 1 h 30 min at RT ...
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bioRxiv - Cell Biology 2022Quote: ... Sections were blocked with PBST (PBS+ 0.05% Tween 20) + 2% BSA for 15 min and incubated 3h at RT with anti-GFP antibody (1:100, 6556 Abcam) in PBST/BSA ...
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bioRxiv - Cell Biology 2019Quote: ... Primary antibodies were incubated overnight at 4°C in 2% milk TBST: anti-CENP-F(Ab5) (1/1500, Rabbit, Abcam), anti-CENP-F(A301-611A ...
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bioRxiv - Immunology 2019Quote: ... samples were incubated in succession in secondary antibody solution for 2 hr at RT (donkey anti-rabbit IgH&L Alexa594, Abcam, ab150076 ...
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bioRxiv - Bioengineering 2019Quote: ... cells were incubated with primary antibodies against microtubule-associated protein 2 (MAP2) (rabbit polyclonal, 1:100, ab32454; Abcam, Cambridge, UK) or Beta III Tubulin (β3TUB ...
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bioRxiv - Immunology 2019Quote: ... Sheared chromatin was then incubated with primary antibodies (AR [06-680, Millipore], H3K4me3 [ab8580, Abcam], phospho-Ser5 RNA polymerase 2 [ab5131, Abcam] ...
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bioRxiv - Immunology 2021Quote: ... plus either rabbit polyclonal anti-SARS-CoV-2 Spike/RBD (Sino, 40592-T62) or rabbit polyclonal anti-CD11b (Abcam, ab52478) for 1 hour at 37°C ...
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bioRxiv - Neuroscience 2020Quote: ... The membranes were blocked with non-fat dry milk and incubated overnight with primary antibodies at 4°C (Abcam polyclonal rabbit IgG: Anti-MMP2 – ab37150, Anti-MMP3 – ab53015, Anti-MMP9 – ab38898, Anti-COX-2 – aDb15191 and Abcam mouse monoclonal anti-beta III Tubulin IgG2a – ab78078 ...
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bioRxiv - Neuroscience 2021Quote: ... and then incubated overnight at 4°C for 24 hours in 2% normal goat serum together with rabbit anti-Nrf2 at 1:500 (Abcam). Slides were then washed and incubated in the secondary antibody (1:1500 ...
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bioRxiv - Molecular Biology 2020Quote: Recombinant antibody generated from the full-length SARS-CoV-2 nucleocapsid protein (product ab272852) and goat anti-human IgG (ab97225) conjugated with horseradish peroxidase (HRP) purchased from Abcam. Nickel coated clear 96-well plates were purchased from Thermo Scientific (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2020Quote: Recombinant antibody generated from the full-length SARS-CoV-2 nucleocapsid protein (product ab272852) and anti-human secondary antibody were purchased from Abcam. Proteins were resolved on a 4-12% Bis-tris gel using SDS-PAGE and transferred to a PVDF membrane (pore size 0.45 µm) ...
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bioRxiv - Neuroscience 2021Quote: ... Lysis buffer (930 µl) and 2 µg of either JADE1 antisera or anti-0N tau antisera (EPR21726, Abcam, Cambridge, MA) were combined and incubated overnight at 4 °C ...
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bioRxiv - Cancer Biology 2020Quote: ... Thermo Fisher Scientific Baltics UAB complies with Quality System Standards ISO 9001 and ISO: 13485) per sample with 2 pg anti-BrdU monoclonal antibody (cat# ab6326, Abcam, Cambridge, UK). Beads were washed four times with 300 pl of PBST wash buffer including SUPERase In RNase Inhibitor (Thermo Fisher ...
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bioRxiv - Neuroscience 2020Quote: ... sections were blocked and incubated with the anti-PrP Fabs at 12 µg/ml and antibody for Microtubule Associated Protein 2 (MAP2) (1∶500, Abcam). Double staining was performed with fluorescently-labeled secondary antibodies (goat anti Human IgG F(ab’)2:Tritc 1:150 ...
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bioRxiv - Plant Biology 2021Quote: ... 2% was removed as input and samples were incubated overnight with 1% antibody (for histone ChIP: anti-H3 (abcam ab1791), anti-H3K27me3 (EMD Millipore 07-449) ...
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bioRxiv - Microbiology 2020Quote: ... paraffin embedded sections were treated with 1:100 rabbit Anti-mouse MLKL (phospho S345) antibody [EPR9515(2)] in order to detect phosphorylated MLKL (Abcam), and 1:100 rat anti-mouse MLKL antibody (Millipore-Sigma ...
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bioRxiv - Neuroscience 2021Quote: ... Primary antibodies were washed five times with PBS before incubation with secondary antibodies at room temperature for 2 h (Alexa 488, 1:1000; abcam). Finally ...
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bioRxiv - Microbiology 2020Quote: ... cells were stained with mouse monoclonal anti-MBL (Biotin) (2 μg/ml in MBL2 wash buffer; 1 h, 37 °C; Abcam) and mouse monoclonal anti-influenza A that had been labeled with Cy3 dye and dialyzed following the manufacturers protocol (1 μg/ml in MBL2 wash buffer ...
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bioRxiv - Cell Biology 2020Quote: ... We prepared bead-antibody complexes by 4 h incubation of protein A/G agarose beads with 2 μg of antibody (anti-histone H3 ab1791; anti-histone H3K4me3 ab8580; both Abcam) in total volume of 500 μL of dilution buffer followed by O/N blocking of antibody-bead complexes ...
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bioRxiv - Cell Biology 2020Quote: ... anti-LAMA 1&2 (ab7463, 1:1000), anti-CD59 (ab133707, 1:1000), and anti-GAPDH (ab9485, 1:2500) were purchased from Abcam.
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bioRxiv - Cancer Biology 2020Quote: Cytosol and mitochondria were separated from A375 cells 2 hr or 4 hr after treatment using the Mitochondria/Cytosol Fractionation Kit (Abcam) following the manufacturer’s instructions.