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Citations for abcam :
2801 - 2850 of 2903 citations for 6H Thieno 2 3 b pyrrole 5 carboxylicacid ethyl ester since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2020Quote: ... and incubated overnight at 4° C with the primary monoclonal mouse antibody MOAB-2 against beta amyloid (1:500; Abcam Cat# ab126649) (Youmans et al. ...
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bioRxiv - Cancer Biology 2020Quote: ... Western blots (2 µg per lane) were probed with primary antibodies specific for CD68 (ab213363, 1/500 in blocking buffer; Abcam, Cambridge, UK) or β-actin (4967S ...
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bioRxiv - Cell Biology 2021Quote: ... lysates of transduced cortical neurons cultured in 6-well plates were were incubated overnight with 2 µl of rabbit α-AP-3µ1 antibody (Abcam, Reference ab201227) on a rotating wheel at 4°C ...
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YAP promotes cell-autonomous immune responses to tackle intracellular Staphylococcus aureus in vitrobioRxiv - Cell Biology 2022Quote: ... The cells were counterstained with 4′,6-diamidino-2-phenylindole (DAPI) for 10 min at 37 °C with or without dye-labeled phalloidin (ab176753 or ab176759, Abcam, Cambridge, UK) for 1 h at 37 °C ...
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Cell-Type Specific Profiling of Histone Post-Translational Modifications in the Adult Mouse StriatumbioRxiv - Neuroscience 2022Quote: ... Nuclei from one striatum (~10,000) were washed 2X and then resuspended in Digitonin Buffer with either anti-H3K4me3 (Antibody 1: Abcam, Ab8580 (Figure 2); Antibody 2 ...
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bioRxiv - Neuroscience 2021Quote: ... diluted in PBS plus 2% NBCS and 0.3% Triton X-100: mouse anti-Glutamate decarboxylase (65 kD isoform, GAD65; 1:1500; ab26113; Abcam, Cambridge, United Kingdom), rabbit anti-Calbindin (1:3000 ...
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bioRxiv - Immunology 2020Quote: ... and equal amounts of fragmented chromatin–protein complexes were incubated for 2 h with enclosed Dynabeads coupled to rabbit anti-Polβ (Ab26343, Abcam, Cambridge, UK), mouse anti-MSH2 mAb (FE11 ...
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bioRxiv - Immunology 2021Quote: ... After 1 hour the wells were washed and Ab binding was detected with Donkey F(ab’)2 Anti-Human IgG conjugated to phycoerythrin (Abcam cat.# ab102439). Cells were analyzed using an iQue High-Throughput Flow Cytometer (Sartorius ...
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bioRxiv - Genomics 2022Quote: ... were washed twice with PBS/BSA and incubated with either 0.5 μl of anti-TBP antibody (gift from Dr. Joseph Reese, Pennsylvania State University) overnight or with 2 μl of anti-H3 (Abcam, cat # ab1791) for 3 hours ...
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bioRxiv - Cell Biology 2019Quote: ... The samples were blocked with 5 % (v/v) FBS in PBS for 30 min and incubated with a rabbit polyclonal anti-COX-2 primary antibody (ab15191, Abcam, Cambridge, UK) diluted at 1:100 in 1 % (v/v ...
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bioRxiv - Plant Biology 2020Quote: ... The supernatant was removed using a magnetic Eppendorf tube rack prior to adding 2 µg of anti-citrulline antibody (ACAb, Abcam, Cambridge, UK) diluted in 200 µL phosphate buffered saline (PBS ...
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bioRxiv - Immunology 2021Quote: ... Cells were then stained for intracellular spike protein using an Anti-SARS-CoV-2 Spike Glycoprotein S1 antibody (Abcam, ab275759, 1:50) followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor 488 ...
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bioRxiv - Evolutionary Biology 2021Quote: ... the sections were incubated overnight (16 h) in a humid chamber at 4°C with either rabbit SHH antibody (2 μg/ml; ab19897; Abcam, Cambridge, UK), mouse Col2 antibody (1/50 dilution ...
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bioRxiv - Immunology 2020Quote: ... Followed by capture of SARS-CoV-2 receptor binding domain which was biotinylated using the lightning-link type-A biotinylation kit(Expedeon/Abcam, 370-0005) for 180s at 10ul/min ...
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bioRxiv - Immunology 2022Quote: ... or CD8+ cells using a rabbit-raised anti-SARS-CoV-2 S protein antibody (1:5000 dilution, ab272504, Abcam plc, Cambridge UK), a rabbit monoclonal anti-CD4 antibody (1:250 dilution ...
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bioRxiv - Microbiology 2022Quote: ... paraffin-embedded sections were firstly subjected to immunostaining with a commercially available and diluted (1:5,000) rabbit-anti-SARS-CoV-2 spike (S) protein (ab272504, Abcam Plc., Cambridge, UK), followed by staining with a peroxidase-conjugated secondary antibody ...
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bioRxiv - Biochemistry 2022Quote: ... The cells were blocked for 35 min by incubation with solution of 1% of BSA in PBS and stained with 2 μg/mL Rabbit Anti-EEA1 antibody - Early Endosome Marker (ab2900, Abcam, Cambridge, UK) for 2 hours at room temperature ...
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bioRxiv - Neuroscience 2022Quote: ... The sections containing hippocampus were blocked with 10% donkey serum with 0.3% Triton X-100 for 2 h at RT and incubation overnight at 4℃ with corresponding primary antibodies: goat anti-IBA-I (1:1000, Abcam, Cambridge, UK), chicken anti-YFP/GFP (1:500 ...
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bioRxiv - Cell Biology 2023Quote: ... the membrane was incubated with goat-anti-mouse IgG secondary antibody conjugated with horseradish peroxidase (ab6789, Abcam, Cambridge, UK; 2 mg/ml) at a final concentration of 0.8 μg/ml / in 1:2500 dilution in milk blocking buffer for 1.5 hours at room temperature ...
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Locomotion-dependent auditory gating to the parietal cortex mediates flexible multisensory decisionsbioRxiv - Animal Behavior and Cognition 2024Quote: ... The samples were incubated at 4℃ for 48 hours with mouse anti-CaMKIIα antibodies (ab22609, abcam, 1:250 dilution in 2% NGS). After treating the primary antibodies ...
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bioRxiv - Neuroscience 2024Quote: ... sections were incubated overnight at 4 ℃ in an antibody diluent (1.1% Triton-X and 2% normal goat serum in PBS) containing primary antibodies anti-TUBB3 (mouse, 1:1000, Sigma-Aldrich, T8660; rabbit, 1:300, Abcam, ab18207), anti-SOX2 (rabbit ...
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bioRxiv - Animal Behavior and Cognition 2024Quote: ... membranes were washed three times and incubated at 4°C overnight in 2% skim milk solution (1g skim milk powder, 50mL TBST) with the primary antibody (GFAP; #ab7260, Abcam, Cambridge, UK) and housekeeper protein (GAPDH ...
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bioRxiv - Bioengineering 2024Quote: ... 100 ug of anti-SARS-CoV-2/S2 (1A9) antibody was conjugated to A647 using the Lightning-Link Conjugation Kit (Abcam, Cat# ab269823) according to the manufacturer’s protocol.
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bioRxiv - Molecular Biology 2022Quote: ... The two fractions were split to two for IgG and YTHDF1 IP (2 μ each, rabbit IgG isotype control, Abcam ab37415; YTHDF1 antibody, Abcam ab220162) and incubated at room temperature for one hour ...
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bioRxiv - Microbiology 2022Quote: Immunohistochemistry was performed on formalin-fixed cryosections of mouse aortic roots using antibodies to identify macrophages (MOMA-2, 1:50; ab33451, Abcam, Cambridge, MA), followed by detection with biotinylated secondary antibodies (1:400 ...
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bioRxiv - Genomics 2023Quote: ... cells were blocked for 1 h in 1×PBS + 2% w/v BSA and then incubated with mouse anti-E-cadherin (1:1000) (Abcam, cat#231303) and chicken anti-vimentin (1:200 ...
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bioRxiv - Immunology 2023Quote: ... H3Cit staining of fixed surface labelled cells was performed by incubating the cells overnight at 4°C in PBS 2% HI-FBS + 2mM EDTA + 0.1% saponin with monoclonal anti-H3Cit antibody (Abcam, ab219407, 1:600). Cells were washed and then incubated with donkey anti-rabbit AF555 (Invitrogen ...
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bioRxiv - Neuroscience 2023Quote: ... The membranes were then incubated overnight at 4°C with the following primary antibodies: anti-COX-2 (Abcam, UK, 1:1000 dilution), anti-IL-1β (Abcam ...
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bioRxiv - Cell Biology 2023Quote: ... Sections were incubated overnight at 4°C with rabbit monoclonal anti-syntaxin 2 antibody (7.8 µg/ml; Abcam, Cambridge, England, UK: ab170852) or goat polyclonal anti- SNAP23 antibody (1.25 µg/ml ...
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bioRxiv - Synthetic Biology 2023Quote: ... listed in Supplemental Table 2 were used to quantify TrxB expression levels with the GFP ELISA kit (Abcam, Cambridge, UK, Cat# ab171581) according to the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2020Quote: ... After blocking with 5% defatted milk, membranes were incubated (overnight, 4°C) with primary antibodies (anti-NNT cat. Abcam: ab110352; β-actin cat. Abcam ab8226). Membranes were then incubated with secondary antibodies conjugated to fluorescent (Licor® ...
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bioRxiv - Cell Biology 2020Quote: ... Primary antibody incubation was performed overnight at 4°C using the following antibodies: anti-GFP rabbit (Abcam, diluted 1:2.000 in 5% milk in TBST) and anti-actin (Sigma ...
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bioRxiv - Immunology 2022Quote: ... table 1) were diluted in blocking solution (F4/80 [Bio-Rad]: 10 µg/ml, Ly6G [abcam]: 1 µg/ml, MHC-II [BioLegend]: 5 µg/ml), CD163 [Invitrogen] ...
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bioRxiv - Developmental Biology 2021Quote: ... Membranes were blocked in 5% BSA-TBST (TBS with 0.1% Tween20) and probed with anti-Padi4 (Abcam ab214810, 1:1000, O/N at 4°C) and anti-Actin (Santa Cruz ...
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bioRxiv - Biochemistry 2022Quote: ... and re-probed (1h, RT, 1/10000 diluted in PBS-Tween with 5% non-fat milk) with rat anti-β actin (AbCam, Cambridge, UK, RRID: AB_867488) washed (as above ...
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bioRxiv - Neuroscience 2022Quote: ... Membranes were blocked with 5% non-fat milk in TBS-T and incubated with primary antibody rabbit Anti-MAP2 (1:1000 dilution, Abcam, Cambridge, MA, catalog no. ab183830). Anti Rabbit-HRP (Invitrogen G-21234 ...
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bioRxiv - Neuroscience 2023Quote: ... Membranes were blocked with a buffer at 4°C overnight prior to being incubated with primary antibodies anti-5-HT1A (Abcam #ab227165, rabbit pAb, 1:1000), anti-5-HT1B (Abcam #ab13896 ...
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bioRxiv - Developmental Biology 2024Quote: ... Brains were incubated in primary antibody (rat anti D-Cad2, DSHB, 1:20, rabbit anti cleaved Dcp-1, CST cat#9578, 1:100, or rat anti Miranda, Abcam CD#5-7E9BG5AF4, 1:500) diluted in 1x PBS 0.3% Triton X-100 overnight ...
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bioRxiv - Cell Biology 2020Quote: ... the primary rabbit mono-specific antibodies were diluted into a concentration of 2-4 µg/ml in blocking buffer (PBS + 4% FBS) containing 1 µg/ml mouse anti-alpha-tubulin (Abcam, ab7291, Cambridge, UK) and chicken anti-KDEL respectively (Abcam ...
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bioRxiv - Immunology 2022Quote: ... Secondary antibodies were applied for 1-2 hours at room temperature and are as follows: Goat α-Rabbit-HRP (Abcam, ab97051, 1:5000), Donkey α-Rabbit-HRP (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2019Quote: ... we used the Maxpar Antibody Labeling Kit (Fluidigm) according to manufacturer’s instructions for addition of the respective metal tags: Axin2 (145-Nd, Abcam, ab32197, 2 µg/ml), p-MEK1/2 [S217/221] (151-Eu ...
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bioRxiv - Cancer Biology 2021Quote: ... Then the sections were blocked in 10% goat serum containing 1% BSA for 2 h at RT and sequentially incubated with primary antibodies: γ-H2AX (ab22551, Abcam; 1:100) or Ki67 (GTX16667 ...
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bioRxiv - Molecular Biology 2022Quote: ... membranes were incubated for 2 h in room temperature with the adequate HRP-conjugated secondary antibody: goat anti-rabbit IgG (1:5000, Abcam, GB; cat. 97051) or goat anti-mouse IgG (1:10000 ...
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bioRxiv - Bioengineering 2022Quote: ... This was followed by a 2-hour incubation with secondary antibody goat anti-rabbit Alexa Fluor 488 (1:500, #ab150077, Abcam, Waltham, MA, USA), and a 0.1% (w/v ...
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bioRxiv - Cell Biology 2022Quote: ... Supernatant was removed into a LoBind tube carefully so as not to remove any beads and 2 µg of anti-GFP antibody (Abcam ab290, anti-rabbit) were added to the sample and incubated overnight at 4°C with rotation ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... e cells were then incubated with primary antibodies against mouse 8-hydroxy-2’-deoxyguanosine (8-OHdG) (1:200)(ab48508, 1:200 dilution; Abcam, Cambridge, United Kingdom) at 4°C overnight ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... For western blot analysis, a human polyclonal IgG anti-SARS-CoV-2 (CIGB, Cuba) and a mouse monoclonal antibody against CK2α (Abcam, Cambridge, United Kingdom) were used as primary antibodies ...
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bioRxiv - Microbiology 2021Quote: ... felis C4 butanol extract were measured with 2’,7’-dichlorofluorescein diacetate (DCFDA) following the manufacturer’s instructions (DCFDA/H2DCFDA Abcam cellular ROS assay kit). Briefly ...
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bioRxiv - Developmental Biology 2022Quote: ... Immunostaining was performed with the following antibodies at 1:50–500 dilutions in blocking solution for 1–2 hours at room temperature: anti-DDDD-K (Abcam, Cambridge, UK, ab1162), anti-GFP (ThermoFisher Scientific ...
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bioRxiv - Microbiology 2020Quote: Paraffin embedded tissue sections were stained for SARS-CoV-2 nucleocapsid protein (1:500) and ionized calcium binding adaptor molecule 1 (Abcam; ab5076; 1:50) using a Leica Bond RXm automated staining instrument following permeabilization using 0.01% Triton X diluted in Trisbuffered saline (TBS) ...