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Citations for abcam :
2451 - 2500 of 2903 citations for 6H Thieno 2 3 b pyrrole 5 carboxylicacid ethyl ester since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: ... Fixed cells were blocked with 5% BSA in PBS for 1 hr at room temperature and incubated with rabbit anti-mouse Ki67 primary antibody (Abcam ab15580) at 1:200 dilution overnight at 4°C ...
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bioRxiv - Cancer Biology 2021Quote: ... Tissue samples were washed with PBS three times for 5 minutes each before adding a secondary goat anti-rabbit IgG H & L (1:1000, Alexa Fluor® 555, Abcam) and Goat Anti-Rat IgG H & L (1:1000 ...
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bioRxiv - Cancer Biology 2022Quote: ... Samples were sectioned in the sagittal plane to a thickness of 5 μm prior to immunoperoxidase staining for Nestin-1 (Abcam, ab237036), a marker that is used to detect vessels in AOM-induced tumors41,42.
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bioRxiv - Neuroscience 2022Quote: ... in a heating chamber (37°C) in which the choroid plexus was loaded with Fluo-8 AM (5 μM, 30 min, Abcam, ab142773) with subsequent continuous wash with HEPES-aCSF (~1 ml/min ...
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bioRxiv - Immunology 2020Quote: ... The transferrin levels in cells were tested by Western blotting using anti-transferrin antibody (1:5□000 dilution, ab82411, Abcam, USA) as described above ...
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bioRxiv - Microbiology 2021Quote: ... Membranes were then washed with TBST buffer (5 × 1 min) and probed with secondary rabbit anti-rat antibodies conjugated with peroxidase (1:1000, Abcam #ab6734). Blots were washed as described above and detection was carried out using Amersham ECL Western Blotting Select Detection Reagent (GE Lifesciences #RPN2235) ...
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bioRxiv - Neuroscience 2022Quote: ... spinal transverse sections 5 mm caudal and rostral from the lesion center were prepared and stained for BDA or PKCγ (Abcam, ab109539). For mice with uPx ...
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bioRxiv - Cell Biology 2022Quote: ... deparaffinized sections were rehydrated and stained using specific antibodies against Leucine-rich repeat-containing G-protein-coupled receptor 5 (Lgr5, ab219107, Abcam, USA), Lysozyme (ab76784 ...
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bioRxiv - Cell Biology 2019Quote: ... embryos and cells were blocked at room temperature in 5% BSA in PBS for 1 hour and incubated with primary antibodies (hCGβ, Abcam, ab9582; TBX3, Abcam, ab99302) in blocking solution overnight at 4°C ...
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bioRxiv - Neuroscience 2019Quote: ... incubated in 5% BSA diluted in TBST for 1hr and then incubated with primary anti-C-terminus GluR1 antibody overnight (Abcam, ab31232). Membranes were washed in TBST (3 × 5 min) ...
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bioRxiv - Cancer Biology 2019Quote: ... using approximately 1×107 SKmel28 clonal CRISPRi cells per assay and 5 μg of the following antibodies: anti-SOX10 rabbit monoclonal antibody (Abcam; ab155279), anti-Histone H3K27ac rabbit polyclonal (Active Motif ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... which was blocked with 5% (w/v) skimmed milk powder in PBS and probed for ERα at a concentration of 1:1000 (Abcam ab108398) and PRβ at a concentration of 1:1000 (Abcam ab46535 ...
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bioRxiv - Neuroscience 2022Quote: ... slices were incubated for 48 hours at 4 °C with primary antibodies diluted in 5% NGS in PBS-T as follows: rabbit anti-cFos (1:1000, Abcam, #190289) and chicken anti-GFP (1:1000 ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were blocked with 5% BSA in PBS for 30 min at room temperature and incubated with anti-Rad51(abcam ab133534) primary antibody at a 1:500 dilution in 1% BSA for 90 min at room temperature or 24 hr at 4°C and was followed by incubation with Alexa Fluor 488 goat anti-rabbit secondary antibody (Invitrogen A11008 ...
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bioRxiv - Developmental Biology 2022Quote: ... Membrane was blocked for one hour in 5% of milk or TBS-T and incubated overnight at 4°C with anti-vinculin (Abcam 73412), anti-CREB (Santa Cruz SC-186) ...
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bioRxiv - Neuroscience 2022Quote: ... and 20 μM ThT in the presence or absence of purified recombinant NRN1 (5 μg/mL or 263 nM; Abcam, ab69755) protein ...
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bioRxiv - Neuroscience 2022Quote: ... The rats received bilateral lesions in the LHb with ibotenic acid (5 µg/µl in 0.01 M PBS, Abcam, Cambridge, UK). The coordinates for the LHb (AP −3.3 mm ...
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bioRxiv - Neuroscience 2024Quote: ... Sections measuring 5 μm were then cut using the Leica RM2165 and subsequently stained with Movat’s Pentachrome staining kit (Abcam Cat# ab245884). For H&E staining analysis ...
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bioRxiv - Neuroscience 2023Quote: ... brain sections were washed in PBS three times (5 min each time) and incubated with a donkey-anti-rabbit Alexa488 secondary antibody (1:1000, Abcam, USA) in PBST for 2 h at room temperature ...
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bioRxiv - Cell Biology 2024Quote: ... membranes were re-stained with rabbit monoclonal anti-SMAD1/5 (SMAD1: 1:500, ab33902; SMAD5: 1:1,000, ab40771; both Abcam, Berlin, Germany) or rabbit monoclonal anti-SMAD2/3 (1:250 ...
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bioRxiv - Developmental Biology 2023Quote: ... Slides were incubated overnight at 4°C with primary antibodies diluted in 5% BSA: rabbit α-SYCP1 (Abcam, ab15090; 1:200) and mouse α- γH2AX (Millipore Sigma ...
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bioRxiv - Neuroscience 2023Quote: ... then the brain slides incubated in a secondary antibody for 90 min protected from light at room temperature (0.1% TritonX-100, 5% normal goat serum, PBS solution with 1:500 goat anti-rabbit Alexa 488; Abcam, Cambridge, MA). Slides were washed again as described above ...
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bioRxiv - Genetics 2023Quote: ... An aliquot of total fragmented chromatin was saved as an input control and the remaining chromatin was split into two tubes (representing 5×107 cells each) for antibody pulldown and incubated overnight at 4°C with 15 ug of NRF1 (Abcam #ab55744), or MYC (Abcam #ab56 ...
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bioRxiv - Genomics 2023Quote: ... 0.1% Tween 20) and incubated over night with the desired antibody diluted in 5% milk / TBST [1:1000 H3 (abcam ab1791); 1:1000 Val1744 cleaved NICD1 (Cell Signaling Technology 4147)] ...
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bioRxiv - Neuroscience 2024Quote: ... The tissues were washed three times with PBS following fixation and blocked with blocking buffer: 5% normal goat serum (NGS, Abcam, AB7681) in PBS with 0.1% Triton-X (PBST) ...
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bioRxiv - Neuroscience 2023Quote: ... The sections were then incubated overnight at 4°C in primary antibody diluted in blocking solution with 5% normal goat serum (Abcam, ab7481). Antibodies were directed against GFP (Abcam ...
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bioRxiv - Cancer Biology 2023Quote: ... Samples were pre-cleared using the beads provided in the kit and incubated overnight at 4°C with 5 µg of commercially available antibodies against lamin A/C (Abcam, #ab108595), phospho-Ser301 lamin A/C (Affinity ...
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bioRxiv - Genomics 2023Quote: ... in PBS-T for 1 hour and incubated overnight in 5% goat serum in PBS-T with primary antibody at 4°C (SON (1:300, abcam ab121759) or SRRM2 (1:300 ...
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bioRxiv - Microbiology 2023Quote: ... washed further 5 times with PBS pH 7.4 and incubated for one hour with Goat anti-monkey (Rhesus macaque) (Abcam cat # a112767), diluted 1:2000 in PBS pH 7.4 ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... paraffin-embedded and 5 µm-thick sections were stained with hematoxylin and eosin (H&E) or picrosirius red solution (Abcam #ab150681) as in [18] ...
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bioRxiv - Systems Biology 2023Quote: ... Primary antibodies were diluted in 5% milk TBST and incubated with blots overnight at 4 °C (FGF1 – Abcam, ab207321; β-ACTIN – Abcam, ab8226 ...
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bioRxiv - Plant Biology 2022Quote: ... pH 7.5) three times, incubated in blocking solution (1% [w/v] bovine serum albumin, 1% [w/v] normal goat serum (Abcam ab7481) in TBS with 0.2% [v/v] Tween-20 [TBST]) ...
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bioRxiv - Molecular Biology 2023Quote: ... Immunoblots were stained overnight at 4°C with the following primary antibodies: anti-ORC1 (1:1000 TBST/5% milk; Abcam, ab85830). Immunoblots were then incubated in secondary goat anti-rabbit (1:1000 TBST/5% milk ...
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bioRxiv - Neuroscience 2023Quote: ... and next incubated for 1 hour in a solution of PB with 5% normal goat serum (Abcam Cat# ab7481, RRID: AB_2716553). Subsequently ...
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bioRxiv - Molecular Biology 2023Quote: ... Cultures were then washed 5x 5 minutes each and incubated with Alexa594-conjugated Goat α-Mouse IgG H&L (Abcam, #ab150116) diluted 1:500 in wash buffer for 1 hour at room temperature ...
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bioRxiv - Immunology 2023Quote: ... 5% non-fat skim milk) and subsequently incubated with a rabbit anti-HIV-1 p55+p24+p17 antibody (Abcam, 1:2000) overnight at 4°C ...
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bioRxiv - Physiology 2024Quote: ... antibody kindly gifted by Jan Nedergaard and Barbara Cannon) and anti-αTubulin to control for equal loading (1:1000 solution in 5% milk in TBS-T, Abcam 2144). The following day ...
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bioRxiv - Cell Biology 2024Quote: ... Primary antibodies were diluted in 5% BSA and incubated at 4°C overnight at given concentrations: α-smooth muscle actin (αSMA, mouse, Cat. No. ab7817, Abcam) at 1:300 dilution ...
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bioRxiv - Physiology 2024Quote: ... Frozen brains were sliced into 7um and incubated in 0.1%PBST + 5%BSA with anti-Iba1 antibody (diluted 1:300, Abcam; Cat#ab178846) and/or anti-GFP antibody (diluted 1:200 ...
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bioRxiv - Cancer Biology 2024Quote: ... ready to use DISCOVERY OmniMap anti-Rb HRP (760-4311) for PHOX2B and Phospho-SMAD-1/5/9 or rabbit anti-mouse secondary (Abcam, ab133469) followed by DISCOVERY OmniMap anti-Rb HRP (760-4311 ...
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bioRxiv - Microbiology 2024Quote: ... The cells were then treated with 5% BSA in PBS for 90 minutes at 25°C followed by treatment with anti-Listeria antibody (Abcam: ab35132) at 25°C for 2 hours ...
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bioRxiv - Physiology 2024Quote: ... incubated with blocking buffer (PBS + 5%FCS +0,1%Tween) for 1 hour and stained with anti-Iba1 antibody (diluted 1:300, Abcam; Cat#ab178846) and /or anti-TOM20 antibody (diluted 1:1000 ...
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bioRxiv - Neuroscience 2024Quote: ... brains and proboscis were washed 5 times for 10 minutes with PB + 0.3% Triton X- 100 (PBT) and blocked for 1 hour in PBT + 5% normal goad serum (NGS) (Abcam, ref. ab7481). Later ...
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bioRxiv - Pathology 2022Quote: ... membranes were incubated for 2 hrs with a secondary Donkey Anti-Rabbit IgG H&L (Abcam; ab16284, 1:10000) or Donkey Anti-Mouse IgG H&L (Abcam ...
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Glyoxal Does Not Preserve Cellular Proteins as Accurately as PFA: A Microscopical Survey of EpitopesbioRxiv - Cell Biology 2019Quote: ... lamin A/C (1:100 in PBS; following blocking with NGS, 2 hr at 37°C) (Abcam; ab108595, USA), or nucleostemin (1:250 in PBS ...
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bioRxiv - Neuroscience 2019Quote: ... Antibodies used were α-syn aggregate-specific antibody MJF14 (rabbit monoclonal MJFR-14-6-4-2. Abcam,1:25,000), the two phospho-serine129-specific antibodies ...
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bioRxiv - Immunology 2019Quote: ... then incubated in secondary antibodies in 0.1% Triton X-100/PBS for 2 hr at 4°C (goat anti-rat Alexa 488, Abcam, ab150157 at 1:500 ...
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bioRxiv - Genetics 2019Quote: ... Dynabeads M-280 sheep anti-rabbit IgG were incubated in lysis buffer and 0.5% BSA for 2 hrs with either rabbit anti-GFP (Abcam) for query IPs or 5 μl of rabbit-anti HA (Abcam ...
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bioRxiv - Cell Biology 2019Quote: ... the samples were incubated with Alexa Flour 488-conjugated goat anti-rabbit antibody (abcam, 1:500 in 2% BSA) and DAPI (Invitrogen ...
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bioRxiv - Bioengineering 2021Quote: ... Cells were resuspended at 1 × 106 cells/mL in cell-culture media supplemented with 2 mM thymidine (Abcam ab143719) and incubated at 37 °C in 5% CO2 for 18 hours ...