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Citations for abcam :
2151 - 2196 of 2196 citations for 5 Bromobenzothiazole 2 sulfonic acid since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... 1:400 dilution) and anti-α- synuclein aggregate antibody (Clone#: MJFR-14-6-4-2; 1:1000 dilution) were acquired from Abcam (Cambridge UK).
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bioRxiv - Neuroscience 2022Quote: ... neurons were incubated first with a blocking solution (BSA 1%, NGS 2%, Triton X-100 0.1%) and then with primary antibodies for KIF1A (Abcam, #ab180153, 1:100, rabbit), HTT (Millipore ...
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bioRxiv - Neuroscience 2022Quote: ... Media was changed 2 h post-infection and the cells were fixed and immunostained 48 h post-infection to detect firefly luciferase (Abcam #ab181640, RRID # AB_2889835).
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bioRxiv - Molecular Biology 2022Quote: ... blocked with blocking solution at 37°C for 3 h and incubated for 2 h with primary antibody (H3K9ac – Abcam ab12179, 1:300) and 1 hour with secondary antibodies (Donkey anti mouse – Abcam ab150109 ...
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bioRxiv - Bioengineering 2024Quote: ... Secondary staining was performed for 2 hours at RT in a PBS (1x) solution containing secondary antibody-TRIC labeled (Abcam, MA, 1:1000), BSA (1% ...
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bioRxiv - Cancer Biology 2023Quote: ... The NOS2 antibody complex was stripped by heating with Bond Epitope Retrieval 2 and then stained with CD8 (Abcam no. 101500, 1:100) or IFNγ (Abcam no ...
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bioRxiv - Cancer Biology 2023Quote: ... The NOS2 antibody complex was stripped by heating with Bond Epitope Retrieval 2 and then stained with CD8 (Abcam no. 101500, 1:100) or IFNγ (Abcam no ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... N-[4-chloro-3-(trifluoromethyl)phenyl]-2-ethoxy-6-pentadecyl-benzamide (CTPB) (CAS #: 586976-24-1) (>99% purity) was purchased from Abcam (Cambridge, United Kingdom). For both chemicals ...
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bioRxiv - Cancer Biology 2024Quote: ... The epithelial nature of the primary cultures and single-cell clones were analysed using a recombinant anti-EpCAM antibody (EPR20533-63; Catalogue number: ab221552, Abcam; Supplementary Figure 2).
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bioRxiv - Neuroscience 2023Quote: ... DRG or spinal sections were incubated with secondary antibodies for 2 h: goat anti-mouse IgG H&L (Alexa Fluor® 488) (1:500, Abcam, Cat#: ab150113), goat anti-guinea pig IgG H&L (Alexa Fluor® 488 ...
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bioRxiv - Immunology 2023Quote: ... cells that were stained with anti-AMPKα1 antibody and unconjugated isotype control antibody were incubated in the dark at room temperature for 2 hours with anti-rabbit Alexa Fluor 488 secondary antibody (Abcam cat. no. ab150113). Following antibody incubation ...
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bioRxiv - Pathology 2024Quote: ... IHC protocol modified from a previously described protocol(37, 38) using a rabbit anti-SARS-CoV-2 S protein antibody (Abcam, ab272504, Cambridge, UK) at 1:5000 dilution (0.2 µg/mL) ...
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bioRxiv - Bioengineering 2024Quote: ... Then the membrane was washed three times in TBS-T and incubated at room temperature for 2 hours with the following secondary antibodies goat anti-rabbit IgG H&L (HRP) (Abcam #ab205718, 1:2000) and m-IgG Fc BP-HRP (Santa Cruz ...
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bioRxiv - Molecular Biology 2024Quote: ... Whole cell lysates of SH-SY5Y cells (10 µg) were subjected to Western blotting using the following antibodies: mouse anti-SLP-2 antibody (Abcam ab89025, 1:1000), rabbit anti-HSP60 (Cell Signaling #4870 ...
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bioRxiv - Physiology 2024Quote: ... and 0.3% Triton X-100 in PBS for 2 h at room temperature and incubated with primary c-FOS antibody (rabbit, Abcam ab190289, 1:1000) overnight at 4°C ...
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bioRxiv - Molecular Biology 2024Quote: ... midguts were incubated at room temperature for 2 hours on an orbital shaker with secondary antibodies (goat anti-rabbit IgG Alexa Fluor 488 [Abcam] at 1:4000) diluted in midgut blocking buffer ...
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bioRxiv - Biochemistry 2024Quote: ... was used as capture antibody and 5nM of Alexa-Fluor 561 labeled pSyn specific antibody and 5nM of Alexa-Fluor 637 labeled Anti-αSyn aggregate antibody [MJFR-14-6-4-2] (Abcam, Cat. No. ab214033) served as detection probes ...
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bioRxiv - Cancer Biology 2021Quote: ... tumor cells were stained using antibodies against chicken anti-GFP (Novus, cat #NB100-1614, concentration 10 μg/mL) and rabbit anti-NR2F1 (Abcam, cat #ab181137, concentration 5 μg/mL), goat anti-SOX9 (RD ...
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bioRxiv - Cancer Biology 2021Quote: ... tumor cells were incubated overnight at 4 C in the presence of primary antibodies against chicken anti-GFP (Novus, cat #NB100- 1614, concentration 100 μg/mL) and rabbit anti-NR2F1 (Abcam, cat #ab181137, concentration 5 μg/mL), or rabbit anti-SOX9 (Millipore ...
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bioRxiv - Cancer Biology 2021Quote: ... Slides were then incubated overnight at 4 C with primary antibodies against chicken anti-GFP (Novus, cat #NB100-1614, concentration 100 μg/mL) and rabbit anti-NR2F1 (Abcam, cat #ab181137, concentration 5 μg/mL), rabbit anti-SOX9 (Millipore ...
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bioRxiv - Molecular Biology 2023Quote: ... The global 5mC of the 30 snails per treatment was measured by the dot blot method based on the recognition of 5 methylcytosine (5mC) by anti-5mC antibody (Abcam, Cat. No. ab73938, Lot: GR278832-3) as described previously (Luviano et al. ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... then incubated overnight at 4°C with the following primary antibodies in 2% w/v BSA: Rabbit anti-Gclc antibody (Abcam, United Kingdom; 1:1000); rabbit anti-NQO1 antibody (Cell Signalling ...
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bioRxiv - Genomics 2020Quote: ... A 1/20 fraction of the supernatant was used as input sample and the remaining material was split for three IP reactions with an anti-H3K9me3 antibody (Abcam, ab8898, lot: GR148830-2). An amount of 4 μg antibody was added to each IP sample ...
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bioRxiv - Developmental Biology 2022Quote: ... Blastocysts were then washed three times in PBS with 2% BSA and then incubated with a secondary anti‐rabbit antibody (1:200, ab150077, Abcam; 1:200, ab150075, Abcam) or anti-mouse antibody (1:200 ...
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bioRxiv - Neuroscience 2022Quote: ... Membranes were blocked with 1×TBST containing 5% dry milk for 2-hr at room temperature and probed with the following antibodies: anti-Kv7.4 antibodies (#ab84820, Abcam, Cambridge, United Kingdom; 1:500), anti-REST antibodies (#ab21635 ...
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bioRxiv - Immunology 2022Quote: ... The membranes were blocked with 5% nonfat dry milk at room temperature for 2 hours and then incubated at 4°C overnight with primary antibodies against β-tubulin (Abcam, Cambridge, MA, USA), PD-L1 (ABclonal ...
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bioRxiv - Biochemistry 2022Quote: ... Then cellular expression of ACE2 or syndecan-1 were detected by rabbit monoclonal antibodies (ACE2, Novus bio, NBP2-67692, 1:500, Syndecan-1, abcam, ab128936 2 μg/mL) and secondary donkey anti-rabbit Alexa Fluor 488 (1:2000 ...
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bioRxiv - Neuroscience 2020Quote: ... incubated in PBST containing 2% NGS with goat anti-rabbit secondary antisera conjugated to Alexa Fluor® 488 (1:1000, Abcam, Cambridge UK, #ab150169) and Alexa Fluor® 647 (1:1000 ...
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bioRxiv - Cell Biology 2023Quote: ... Cells lysates were either mixed with 3 × Laemmli buffer for immunoblotting analysis or subject to pre-clearing and NDC80 complexes were isolated from 1 mg of cell lysate by 2-h incubation at 4°C with anti-NDC80 antibody cocktail (ProteinTech, 18932-1-AP; Abcam, ab3613; SantaCruz, sc-515550) or ...
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bioRxiv - Microbiology 2022Quote: ... The higher molecular weight part of the membrane was incubated overnight at 4ºC in anti-DNAK antibody (1:5000 dilution in blocking buffer, Abcam#69617 Mouse monoclonal [8E2/2]) as the loading control ...
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bioRxiv - Neuroscience 2023Quote: ... Membranes were then blocked in 5 % milk in 1X TBS-T for 1 hour at room temperature followed by overnight incubation in primary antibody against pSer129 (pS) α-Syn (1:2 000, Abcam, 51253, Cambridge, UK), α-Syn (1:2 000 ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... Embryos were then fixed in 4% paraformaldehyde overnight and immunohistochemistry was performed according to previous protocols (Dasgupta et al., 2019) with minor modifications using an anti-acetyl H3K27 – primary antibody (Abcam, AB177178; 2 µg/mL) and a goat-anti-rabbit IgG secondary antibody (Sigma Aldrich ...
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bioRxiv - Neuroscience 2024Quote: ... before blocking in 10% BSA and incubation with primary anti-bodies against aggregated α-synuclein (MJF-14; rabbit MJFR-14-6-4-2, Abcam, #ab209538, 1:25,000), total α-synuclein (mouse LB509 ...
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bioRxiv - Neuroscience 2024Quote: ... fol-lowed by 5% horse serum before incubation with the primary antibody MJF-14 (anti-conformation-spe-cific α-synuclein filament antibody MJFR-14-6-4-2, Abcam, #ab209538, 1:200). The immunohistology sig-nals were visualized with ImmPRESS®-AP Horse Anti-Rabbit IgG Polymer (VectorLabs ...
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bioRxiv - Neuroscience 2024Quote: ... in PBS before incubation with the primary antibody MJF-14 (anti-confor-mation-specific α-synuclein filament antibody MJFR-14-6-4-2, Abcam, #ab209538, 1:25,000), overnight at 4°C ...
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bioRxiv - Neuroscience 2021Quote: ... DR sections (from −5.0 mm to −4.0 mm from bregma in the anterior-posterior axis) were co-stained with primary antibodies against tryptophan hydroxylase-2 (goat α-TPH2, Abcam 121020, at 1:400) as a marker of serotonergic neurons and vesicular glutamate transporter-3 (guinea pig α-VGLUT3 ...
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bioRxiv - Cell Biology 2024Quote: ... Immunoblotting was next performed by incubating the membranes overnight at 4°C with the following primary antibodies: mouse monoclonal anti-mitofusin 2 antibody [6A8] (abcam, ab56889, dilution of 1/1000), rabbit polyclonal anti-mitofusin 2 (abcam ...
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bioRxiv - Neuroscience 2024Quote: ... United States).The following day slices were washed 4 × 10 min followed by 2 h incubation in secondary antibody solution containing goat-anti-rabbit Alexa Fluor 488 (1:500; Abcam, ab150077, Waltham, MA, United States), goat-anti-chicken Alexa Fluor 488 (1:500 ...
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bioRxiv - Cell Biology 2024Quote: ... Rabbit polyclonal HPA antibodies targeting the proteins of interest were dissolved to 2–4 μg/ml in blocking buffer (PBS + 4% FBS) containing the cilia marker antibodies against ARL13B (Abcam, ab136648, mouse IgG2a, 1:2000) and PCNT (Abcam ...
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bioRxiv - Pathology 2020Quote: ... Primary antibodies were incubated in blocking buffer for 2 h at room temperature (rabbit anti-ACE2 [Abcam #ab15348, used at 1:500 dilution]; mouse anti-GAPDH [Abcam #ab8245, used at 1:3,000 dilution]). Membranes were washed and further incubated with fluorescent dye-conjugated secondary antibodies for 1 h at room temperature (AlexaFluor 647 goat anti-rabbit IgG1 and AlexaFluor 488 goat anti-mouse IgG1 ...
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bioRxiv - Genetics 2021Quote: ... This was centrifuged and supernatant was collected into a new tube and incubated with anti-H3K27 antibody (ab177178, Abcam, 2 μg for 25 μg of chromatin) or anti-H3K4me1 antibody (ab8895 ...
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bioRxiv - Neuroscience 2023Quote: ... 1% bovine serum albumin in PBS with 0.1% Tween20 [PBST]) for one hour at RT and left overnight in the A2AR primary antibody at 4°C (Abcam #ab3461, Lot # GR3431019-2, 1:100) in blocking solution ...
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bioRxiv - Neuroscience 2024Quote: ... the slices were rinsed three times in blocking solution and then incubated for 2 h at room temperature with the following secondary antibodies: goat anti-chicken Alexa Fluor 488 (Abcam, catalog no. ab150169, dilution 1:1000) and goat anti-rabbit iFluor 555 (AAT Bioquest ...
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bioRxiv - Cell Biology 2021Quote: ... Non-specific interactions were blocked for 1 hour with blocking solution (3% BSA, Sigma ref. A9647, 2% donkey serum, Abcam ref. ab7475, 0,3% Triton X-100, PBS). Primary antibodies were diluted in the blocking solution and incubated overnight at 4°C ...
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bioRxiv - Neuroscience 2020Quote: ... Tissue was then rinsed in PBS three times for 10 min each and incubated for 2 hr in secondary antibodies (goat anti-rabbit Alexa Fluor® 405 for the PV antibody, (abcam, Cat# ab175652, RRID AB_2687498, 1:500), goat anti-mouse Alexa Fluor® 594 for the 8-oxo-dG antibody (abcam ...
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bioRxiv - Molecular Biology 2024Quote: ... cell lysates were prepared using SDS sample buffer followed by Western Blotting and antibody probing procedures according to the guidelines of the company for the respective antibodies (anti-PPARα: H-2, sc-398394, 1:1000, Santa Cruz Biotechnology; anti-CPT1α: ab128568, 1:1000, Abcam; anti-ERRα: ab137489, 1:1000, Abcam). Imaging was done using KODAK films or the Amersham Imager 680 equipment.