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Citations for abcam :
1651 - 1700 of 2196 citations for 5 Bromobenzothiazole 2 sulfonic acid since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2024Quote: ... Serine racemase treatment of human serum was performed with the addition of 5 µl of proprietary serine racemase solution from a DL-serine assay kit (Abcam) to 1 ml of serum for 3 h ...
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bioRxiv - Neuroscience 2024Quote: ... cells were incubated with 20 μM NE for 5 min and an aliquot of saline was used to measure adenosine using an adenosine assay kit (Abcam) and a microplate reader (Perkin Elmer Enspire ...
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bioRxiv - Molecular Biology 2024Quote: ... Blots were subsequently washed 3 times for 5 minutes each in PBST at room temperature before incubation in 5µg/mL of HNRNPA2B1 polyclonal primary antibody (abcam 31645) in PBST overnight at 4°C with agitation ...
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bioRxiv - Physiology 2024Quote: ... Sections were placed in 100% propylene glycol for 5 minutes then incubated in pre-heated Oil Red O (Abcam #150678) for 6 minutes at 60°C ...
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bioRxiv - Microbiology 2024Quote: ... The membrane was incubated with the primary antibody (RPS3, abcam, Cat#ab128995; eIF4E, abcam, Cat#ab33768; ORF3a, abcam, Cat#280953; 5-mC, Cat#ab214727; IFN-beta, abcam, Cat#ab275580 ...
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bioRxiv - Microbiology 2024Quote: ... unskimmed milk powder, and incubated with mouse monoclonal anti-HA (clone 6E2, Cell Signalling Technology) and anti-p24 (clone 5, Abcam) overnight at 4°C ...
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bioRxiv - Microbiology 2024Quote: ... To test for O-x-AlkTDM presence we used in-gel fluorescence (to detect TAMRA) and/or immunoblotted with anti-biotin antibodies (1:1000; 5% nonfat Milk in TBST; Abcam).
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bioRxiv - Immunology 2024Quote: ... ∼50 µg of lysate material was treated with the reducing agent TCEP at a final concentration of 5 mM and heated to 60 °C for 30 minutes before being loaded onto a FASP protein digestion column (Abcam)75 ...
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bioRxiv - Immunology 2024Quote: ... Slides were washed 3 x 5 min and stained with 1:1 HRP-conjugated goat anti-rabbit IgG (Abcam ab214880) in 1% BSA-TBS for 2 hours at room temperature ...
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bioRxiv - Cell Biology 2024Quote: ... The membranes were blocked with 5% milk followed by incubation with primary antibodies overnight using the following antibodies: anti-VEGF-A (Abcam), anti-BCL2 (Cell Signaling Technology) ...
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bioRxiv - Molecular Biology 2024Quote: ... Wells were again washed three times for 5 minutes with dPBS before incubating with secondary antibodies (goat anti-rabbit IgG Alexa Fluor 488 [Abcam] ...
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bioRxiv - Cancer Biology 2024Quote: ... or with recombinant TGF-β at 10 ng/mL (stock concentration 5 ng/µL) or Batimastat at 30 µM (stock concentration 1 mM, BB-94/Abcam). After 48 hours (or 1 hour for SMAD activity tests) ...
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bioRxiv - Molecular Biology 2024Quote: ... 3 µl of 1 mM DCFDA dye in 1X buffer and 5 µl of 10 µM DiOC2 was added for ROS evaluation (ab113851, Abcam) and membrane depolarization (B34950 ...
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bioRxiv - Neuroscience 2024Quote: ... Sections were then washed for 5 mins in PBS and mounted with mounting media containing DAPI (catalog number ab104139, Abcam) before imaging.
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bioRxiv - Bioengineering 2024Quote: ... the blots were washed with PBS supplemented with 0.1% Tween 20 four times for 5 min before staining with the secondary antibody (7076, Cell Signaling; ab216773, Abcam) in blocking buffer with 0.1% Tween 20 for 1h at room temperature ...
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bioRxiv - Physiology 2024Quote: ... The membranes were blocked (5% milk (PanReac AppliChem, A0830)) and probed with the following antibodies: anti-β-Tubulin (Abcam, ab15568), anti-GAPDH (Proteintech ...
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bioRxiv - Bioengineering 2024Quote: ... cells were washed 3 times for 5 min with PBS before incubating with a secondary antibody (ab150080 and ab150157, Abcam) for 1 h at room temperature ...
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bioRxiv - Cancer Biology 2024Quote: ... All of supernatant containing digested nuclear extract was transferred to a new tube and 500µl of Low IP buffer without additional NaCl or detergent was added together with 5-10µg of KAT7 antibody (Abcam #190908) or rabbit IgG (R&D Systems ...
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bioRxiv - Bioengineering 2024Quote: ... for 5 minutes and then incubated for 1 hour at room temperature (RT) in NM95 primary antibody (ab190710 16 - Abcam) at 1:1500 in TBS ...
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bioRxiv - Cancer Biology 2024Quote: ... Membranes were blocked with 5% milk-TBST for 1 hour under agitation and probed overnight for ALIX (1:1000, 186429, Abcam), Calnexin (1:1000 ...
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bioRxiv - Cell Biology 2024Quote: ... The primary antibody was washed 2x with PBS for 5 min per wash and cells were incubated with secondary antibodies (diluted in 0.5% BSA/PBS) at room temperature in the dark for 1 h at 1:1000 dilution (anti-rabbit Alexa488, Abcam ab150077 ...
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bioRxiv - Cancer Biology 2021Quote: ... Multi-color immunohistochemistry was conducted on a cohort of early-stage non-small cell lung cancer patients as described elsewhere (https://jitc.bmj.com/content/9/2/e001469) with polyclonal rabbit anti-Neutrophile Elastase antibody (Abcam, Cambridge ...
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bioRxiv - Cell Biology 2020Quote: ... Immunohistochemistry was executed on human colonic tissue using antibodies against Epac-2 and Rap-1 (1:100; Abcam).
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bioRxiv - Cell Biology 2020Quote: ... Western blot analysis was performed using 100 μg of total protein using anti-ACE2 (Abcam/ab239924/EPR4435(2)) ...
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bioRxiv - Genetics 2021Quote: ... Lysates were precleared and then immunoprecipitated with 2 μl anti-histone H3 (monomethyl K9) antibody (Abcam no. ab9045), 2 μl anti-histone H3 (dimethyl K9 ...
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bioRxiv - Neuroscience 2022Quote: ... Slices were then washed and developed for 2-3 min using 3,3′-diaminobenzidine (DAB) staining kit (Abcam; #ab64238;). Nuclei were counterstained using Haematoxylin (MHS32-Sigma ...
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bioRxiv - Cancer Biology 2020Quote: ... pancreatic or lung CAFs were treated with an inhibitor to beta-site Amyloid precursor protein cleaving enzyme 1 and 2 (BACE) (0.7μM; Abcam) for the duration of CAF CMed generation to inhibit Amyloid β production by the cells.
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bioRxiv - Genomics 2021Quote: ... and sheared DNA was added to antibodies coupled to washed magnetic beads (anti-FLI1 2 µg, ab15289, Abcam; anti-ERG 2 µg ...
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bioRxiv - Neuroscience 2021Quote: ... and incubated with primary antibodies diluted in PBS containing 2% NHS overnight (1:500 rabbit anti-Iba1, Abcam, ab178846 ...
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CAP1 and cofilin1 cooperate in neuronal actin dynamics, growth cone function and neuron connectivitybioRxiv - Neuroscience 2020Quote: ... 10% donkey serum and 0.5% NP40 in PBS and stained over night at 4°C with following primary antibodies in 2% BSA and 0.5% NP40 in PBS: rat anti-Ctip2 (1:1,000, Abcam), rabbit anti-Neurofilament200 (1:80 ...
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bioRxiv - Cell Biology 2021Quote: ... lysed using 62.5 mM Tris buffer (pH 6.8) containing 2% SDS and used for protein quantification using BCA assay (Abcam). Briefly ...
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bioRxiv - Neuroscience 2020Quote: ... and either vehicle or 3-((2-Methyl-4-thiazolyl)ethynyl) pyridine (MTEP) hydrochloride (Abcam Biochemicals, Cambridge, MA, USA) were infused over 2 min ...
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bioRxiv - Genomics 2022Quote: ... The Rabbit IgG control antibodies for ChIP-qPCR experiments were ab172730 (abcam, GR3298823-2, 2ug per 80ug chromatin), and Normal IgG Rabbit 2729 (Cell Signaling ...
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bioRxiv - Bioengineering 2022Quote: ... n=2-3) samples was performed according to manufacturer instructions of the Alkaline Phosphatase Assay Kit (colorimetric) (abcam).
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bioRxiv - Molecular Biology 2022Quote: ... The slides were then stained for 2 minutes 30 seconds with a DAB substrate kit (Abcam ab 64238) and washed in tap water twice prior to incubating in tap water for 2 hours prior to cover slipping.
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bioRxiv - Genomics 2022Quote: ... and incubated overnight at 4°C in either anti-dsDNA (Abcam, 1:5,000 in 2% BSA in TBST) or anti-5-methyl-cytosine (5mC – Aviva Biosystem clone 33D3 ...
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bioRxiv - Microbiology 2021Quote: ... cells were stained with the primary rabbit polyclonal anti-SARS-CoV-2 spike glycoprotein antibody (1:1000, Abcam) for 1 h at RT or overnight at 4 °C ...
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bioRxiv - Developmental Biology 2022Quote: ... The blastocysts were incubated with primary antibodies for 8-Hydroxy-2-deoxyguanosine (8-OHdG, 1:200; ab48508, Abcam), 8-iso Prostaglandin F2 alpha (PGF2 alpha ...
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bioRxiv - Developmental Biology 2020Quote: ... Blocking was performed with 2% BSA in 0.5% Triton/PBS and rabbit polyclonal anti-mCherry primary antibody (Abcam) was used in 1:500 dilution overnight at 4°C ...
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bioRxiv - Neuroscience 2021Quote: ... Grids were incubated for 2 h in the presence of anti-LRRK2 (1:20 dilution, Abcam, cat. # ab133474) and anti-CD63 antibodies (1:40 dilution) ...
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bioRxiv - Immunology 2021Quote: Sonicated chromatin samples of 20,000 cells per assay were profiled for H3K27ac (abcam, cat: ab4729, lot: GR312651-2) with MOWChIP-seq as described in our previous publications53,58 ...
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bioRxiv - Immunology 2021Quote: ... the cells were marked with 4′,6-diamidino-2-phenylindole (DAPI) (cat. n° ab104139, Abcam, Cambridge, MA, USA), and incubated for 5 min ...
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bioRxiv - Neuroscience 2020Quote: ... mice received intraperitoneal injections (2 mg/kg) of each of the endothelin receptor antagonists BQ-123 (ab141005, Abcam) and BQ-788 (ab144504 ...
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bioRxiv - Cancer Biology 2021Quote: ... USA) and oligodendrocyte lineage transcription factor 2 (OLIG2; clone EPR2673, rabbit monoclonal, dilution 1:50, Abcam, Cambridge, UK).
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bioRxiv - Cancer Biology 2021Quote: ... Cells were then incubated with goat F(ab’)2 anti-Human IgG Fc-FITC antibody (Abcam, Cambridge, MA) for 30 minutes in the dark on ice ...
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bioRxiv - Cell Biology 2021Quote: ... for 2 h at 4°C followed by incubation with primary antibody to TCFL5 (1:50; 188075, Abcam) or control IgG (normal rabbit IgG ...
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bioRxiv - Cancer Biology 2021Quote: ... they were then washed and incubated with primary antibodies against Bcl-2 (1: 1000, #7973; Abcam, Cambridge, MA), caspase-3 (1:2000 ...
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bioRxiv - Developmental Biology 2023Quote: ... 1 Million cells were used and incubated with 2 µg YAP antibodies (Abcam ab52771, Santa Cruz sc-101199). All following steps were performed as described43,44 ...
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bioRxiv - Microbiology 2023Quote: ... The immunohistochemistry technique was performed with a commercial antibody anti-SARS-CoV-2 spike protein (Abcam, Cambridge, UK) and the in-house mAb 1F7 ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... and incubated overnight at 4°C in either anti-dsDNA (Abcam, 1:5000 in 2% BSA in TBST) or anti-5-methyl-cytosine (5mC – Aviva Biosystem clone 33D3 ...