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Citations for abcam :
1951 - 2000 of 2196 citations for 5 Bromobenzothiazole 2 sulfonic acid since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2024Quote: ... Patient IgG4 was detected using 25 ng/mL of an HRP-conjugated goat F(ab’)2 anti-human IgG-Fc polyclonal antibody (Abcam) in assay buffer for one hour at room temperature followed by adding TMB substrate ...
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bioRxiv - Animal Behavior and Cognition 2024Quote: ... the sections were incubated with primary antibodies SOX 2 Mouse (Sta. Cruz) with a dilution of 1:200 and Ki-67 rabbit (Abcam) with a dilution of 1:200 ...
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bioRxiv - Neuroscience 2024Quote: ... for 30 min at room temperature and then blocked in a solution of 0.3% PBS-T and 5% normal donkey serum (NDS) for 2 hours before being incubated with primary antibodies (1°Abs included: chicken anti-GFP [1:1000, Abcam, ab13970] ...
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bioRxiv - Cancer Biology 2024Quote: ... Sheared chromatin was diluted to contain 0.16% SDS for antibody binding and incubated overnight at 4 °C with 2 ug anti-H3K27Ac (Abcam, ab4729), anti-H3K4ME3 (Cell Signaling Technology ...
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bioRxiv - Bioengineering 2024Quote: ... Primary antibody solution for Aldh1l1 and vimentin staining: 2 mL blocking buffer containing 20 μL anti-Aldh1l1 antibody (Abcam, AB56777) and 20 μL anti-vimentin (Abcam ...
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bioRxiv - Neuroscience 2024Quote: ... Assays were performed using the confor-mation-specific α-synuclein antibody MJF-14 (MJFR-14-6-4-2; Abcam, #ab214033) or the total α-synu-clein antibody syn211 (Abcam ...
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bioRxiv - Molecular Biology 2024Quote: ... Cells were rotated for 2 hours at 4 °C in 200 μL D-Wash with 1:200 LMNB2 (Abcam, ab8983) followed by a wash step with D-Wash ...
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bioRxiv - Bioengineering 2024Quote: ... The primary antibodies and respective concentrations used in this study are the following: anti-Ki67 (2 µg/mL, ab15580, abcam), anti-p21Cip1/Waf1 (0.48 µg/mL ...
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bioRxiv - Neuroscience 2024Quote: Dot blots were used to confirm immunoreactivity of generated PFFs with a commonly used conformation-specific anti-αSyn antibody ([MJFR-14-6-4-2], Abcam). 1.5 µl of serially diluted monomer or (un)sonicated PFF samples were loaded onto nitrocellulose membrane (0.45 µm ...
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bioRxiv - Neuroscience 2024Quote: ... brain tissue was quantified by dot blot (described above) using a conformation-specific anti-αSyn antibody ([MJFR-14-6-4-2], Abcam), and diluted PFFs as a standard series ...
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bioRxiv - Neuroscience 2024Quote: ... for 2 hrs at room temperature before incubation with primary antibody (1:500, conformation-specific anti-αSyn antibody [MJFR-14-6-4-2], Abcam) in blocking solution overnight at room temperature ...
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bioRxiv - Neuroscience 2024Quote: ... and Alexa Fluor 488-conjugated anti-chicken secondary antibody (goat, 2 mg/ml, dilution 1:500; catalog no. ab150169, Abcam) were used for GFlamp2 and ChR2-EYFP.
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bioRxiv - Neuroscience 2024Quote: ... for 2 h and immediately incubated with a rabbit anti-c-Fos antibody (1:2,000; ab190289, abcam, Cambridge, UK; RRID:AB_2737414) at RT overnight (16 h) ...
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bioRxiv - Cell Biology 2024Quote: ... cells were incubated for 1-2 h at room temperature with the indicated primary antibodies: rabbit anti-lamin B1 (ab16048, Abcam), mouse anti-separase (6h6 ...
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bioRxiv - Biophysics 2024Quote: ... The cells were then washed twice in ice-cold PBS and blocked for 30 minutes in PBS containing 2% donkey serum (ab7475, Abcam), 300 mM glycine (50046 ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... the dissected intestines were incubated for 2 hours at 25°C in antibodies against GFP (Abcam, Waltham, MA; Cat#: ab6556) and nuclear pore complex (NPC ...
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bioRxiv - Cell Biology 2024Quote: ... the cells were washed three times with PBS and stained with 2 μg/mL Alexa488-labeled secondary antibody for mouse IgG (ab150077, Abcam) for 30 min at room temperature ...
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bioRxiv - Cancer Biology 2024Quote: ... 2 mg of protein lysate was incubated overnight at 4°C with 2 µg of MHC-I mouse antibody (ab70328, Abcam) or mouse IgG1 isotype control antibody (5415 ...
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bioRxiv - Cell Biology 2024Quote: ... Samples were blocked in 10% normal goat serum + 5% DMSO in PBT overnight at 4°C then incubated at 4°C for 24h in primary antibodies (1.25 µg/ml E7 anti-β tubulin (DSHB) and 2 µg/ml ab1791 anti-H3 (abcam) in PBT + 10% normal goat serum) ...
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bioRxiv - Neuroscience 2021Quote: ... Mounted sections were dehydrated in 100% propylene glycol for 5 min and then stained with Oil Red O (ORO) for 10 min (Abcam, Cat. No. ab150678). The sections were then differentiated in 85% propylene glycol for 3 min and rinsed with distilled water three times ...
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bioRxiv - Molecular Biology 2021Quote: ... Membranes were then blocked (5% milk in TBS-Tween buffer) and incubated for 18 h with primary antibodies: CD44 (Abcam, 1:1000 dilution); GFAP (ThermoFisher ...
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bioRxiv - Biochemistry 2021Quote: ... PAs were then permeabilized with 0.2% Triton X for 30 minutes at room temperature followed by blocking with 5% normal donkey serum (Abcam plc, Cambridge, MA, USA) and 300 mmol/L glycine for one hour at room temperature ...
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bioRxiv - Immunology 2022Quote: ... PVDF membrane was blocked with 5% skim milk buffer and then incubated with one of the following primary antibodies: mouse anti-HSP70 (ab2787, Abcam, Cambridge, MA, USA), rabbit anti-TSG101 (14497-1-AP ...
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bioRxiv - Cell Biology 2022Quote: ... Samples were blocked with 5% BSA and then incubated with the antibody solution (anti-pMLKL 1:200, Abcam; anti-Iba1 1:700, Wako), overnight at 4 °C ...
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bioRxiv - Molecular Biology 2020Quote: ... in a Tris-glycine-methanol transfer buffer overnight and detected with the following antibodies: 1:5000 monoclonal mouse anti-Hsp70 [C92F3A-5] (Abcam Cat# ab47455, RRID:AB_881520) (abcam) ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Membranes were then probed to determine protein levels using the following antibodies diluted in 5% non-fat milk in 1x TBST: matrix metalloproteinase 9 (MMP9) (Abcam ab38898, 1:1000) and MMP2 (Abcam ab92536 ...
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bioRxiv - Developmental Biology 2022Quote: ... Membranes were incubated in blocking buffer containing (TBST+5% BSA) for 1hour at RT and incubated with primary antibody in blocking buffer at 4°C overnight: POLD1 (Abcam Cat# ab168827,1:500); GAPDH (Santa Cruz Biotechnology Cat# sc-32233 ...
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bioRxiv - Bioengineering 2020Quote: ... and PBS (twice, 5 min each) and left for 1 hour in block solution containing 10% goat serum (ab7481, Abcam, Cambridge, United Kingdom), 0.25% Triton-X 100 (Sigma Aldrich) ...
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bioRxiv - Physiology 2020Quote: ... Nuclear sirtuin 1 content and activity was assessed using 10 µg (5 μL) of isolated nuclear protein fractions through a fluorometric assay (Sirt1 Activity Assay Kit, #ab156065, Abcam, Whitby, Ontario, Canada). Another set of samples weighing 5-10 mg were homogenized in RIPA buffer (150 mM sodium chloride ...
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bioRxiv - Molecular Biology 2021Quote: ... H4K20me1 or H4K20me3 IP was performed overnight in TD buffer as described above using 5 µg of H4K20me1 or H4K20me3 antibody (Thermo Fisher Scientific # MA5-18067, Abcam # ab9053 respectively). After antibody capture on protein G magnetic beads and 3 washes with TD buffer ...
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bioRxiv - Biochemistry 2021Quote: ... then incubated in 5% milk in TBS-T containing 1:10000 horseradish peroxidase (HRP) conjugated goat anti-mouse secondary antibody (Abcam ab6789-1 MG) for 1 hour at room temperature ...
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bioRxiv - Neuroscience 2020Quote: 5-FU and wdr12 samples were immunolabeled with anti-HuC/D antibodies (not shown) (ab210554, abcam, used at a dilution of 1:600). Anti-GFP (GFP-1020 ...
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bioRxiv - Cancer Biology 2020Quote: ... the membranes were placed in 5% nonfat milk for blockage and probed with the primary antibodies against TEAD4 (ab151274, Abcam, Cambridge, MA, USA), PKM2 (ab137852 ...
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bioRxiv - Molecular Biology 2023Quote: ... Primary antibodies were incubated overnight at 4C at the following dilutions in a 5% serum buffer in PBS: LCK (ABCAM Ab227975, 1:200), FYN (BD Biosciences BD61063 1:150) ...
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bioRxiv - Cell Biology 2023Quote: ... Membranes were incubated either at room temperature for 2 hours or overnight at 4°C with primary antibodies in 5% milk in 1X TTBS at the following concentrations: DNAJB6 (Abcam #ab198995; 1:1000), CTCF (Cell Signaling Technology #2899 ...
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bioRxiv - Neuroscience 2022Quote: ... Co-staining of astrocytes and endothelial cells were performed with antigen retrieval (boiling in sodium citrate buffer in pressure cooker for 5 min) and probing with GFAP-488 (eBioscience 53-9892-80, 1:400) and CD31 (Abcam 28364, 1:200).
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bioRxiv - Cancer Biology 2024Quote: Immunofluorescence (IF) was performed on whole FFPE 5-µm-thick tissue sections using anti-TP63 mouse monoclonal antibody (1:100; Abcam, catalog no. ab735), anti-CK8 rabbit polyclonal antibody (1:100 ...
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bioRxiv - Neuroscience 2023Quote: ... Sections were incubated at 4°C for 72 hours in blocking solution (5% bovine serum albumin, 0.2% Triton-X) containing primary antibodies CD45 antibody (Abcam, ab10558, 1:400 dilution), CD11b (Abcam ...
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bioRxiv - Neuroscience 2023Quote: ... The membranes were blocked with 5% non-fat milk for 1 h and incubated with primary antibodies against β-actin (1:1000, Abcam, cat# ab8226), PI3K (1:1000 ...
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bioRxiv - Bioengineering 2022Quote: ... Each well was blocked with 5% BSA-tween solution and incubated with a primary mouse anti-human α-SMA antibody (ab7817, Abcam, Cambridge, UK) solution at a 1:100 dilution overnight at 4°C ...
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bioRxiv - Cell Biology 2023Quote: ... After washing the membranes in TBST (3 × 5 min), they were probed (1.5 h, RT) with the HRP-conjugated secondary antibodies: goat anti-mouse IgG (Abcam Cat# ab205719, 1:10000) or goat anti-rabbit IgG (Abcam Cat# ab205718 ...
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bioRxiv - Neuroscience 2024Quote: ... The following antibodies were purchased from the manufacturers described: mouse anti-tau antibody (tau-5; Cat# ab80579) and rabbit anti-GAPDH antibody (Cat# ab181602) from Abcam (Cambridge, United Kingdom), rabbit anti-tau polyclonal antibody (Cat# A0024 ...
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bioRxiv - Cancer Biology 2024Quote: ... After blocking with 5% skim milk (Beyotime, China) and sequential incubation with the primary and secondary antibodies: anti-PRMT3 (Abcam, Ab191562, 1:2000), anti-PRMT3 (Proteintech ...
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bioRxiv - Cancer Biology 2024Quote: ... The membranes were then blocked using 5% nonfat milk for 1 h and incubated with primary antibodies against VASN (cat# ab156868, abcam, 1:500 dilution), IL33 (cat# A8096 ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were fixed in 2% formaldehyde in PBS for 10 min at room temperature and stained with Phalloidin-iFluor 594 (abcam, ab176757) for 30 minutes at room temperature ...
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bioRxiv - Cell Biology 2020Quote: ... the chromatin fraction was solubilized in EBC-150 buffer with 500 U/mL Benzonase and 2 ug of antibody directed to endogenous RNAPII-S2 (Abcam; ab5095) or RNAPII-S5 (Abcam ...
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bioRxiv - Cell Biology 2020Quote: ... Unbound primary antibody was removed the following day by washing in 0.2% Tween 20 in PBS and blastocysts incubated for 2 hr at RT with goat anti-rabbit Alexa-488 conjugated secondary antibodies (Abcam, ab150077). Following washing ...
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bioRxiv - Developmental Biology 2021Quote: ... slides were incubated with secondary antibody for 2 h at room temperature before DAB reaction using the DAB detection kit (Abcam #ab64238). Slides were counterstained with hematoxylin to stain nuclei ...
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bioRxiv - Developmental Biology 2020Quote: ... Slides were washed in PBST as above to remove the excess of the secondary antibody and then one drop of Fluoroshield Mounting Medium with 4’,6-diamidino-2-phenylindole (DAPI) (Abcam, #ab104139) was added on the immunostained samples.
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bioRxiv - Neuroscience 2021Quote: ... end stage high- titer viral aliquots or 2) from cell culture media used for rabies virus rescue after PEG- based precipitation (Abcam, ab102538) and quantified using the High Sensitivity RNA ScreenTape assay (Agilent ...