Labshake search
Citations for abcam :
2001 - 2050 of 2129 citations for hsa mir 483 5p Real time RT PCR Detection Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2020Quote: Transferrin and cytokines in the samples (cell supernatant or plasma) were measured using a mouse transferrin ELISA kit (ab157724, Abcam, USA), mouse TNF-α ELISA kit (DKW12-2720-096 ...
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Development of a small molecule that corrects misfolding and increases secretion of Z α1-antitrypsinbioRxiv - Pharmacology and Toxicology 2020Quote: ... Immunohistochemistry for polymerised α1-antitrypsin was performed using 2C1 monoclonal primary antibody and a Mouse-on-Mouse Polymer kit (Abcam AB127055) for detection ...
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bioRxiv - Cancer Biology 2020Quote: Mammary glands were dissected and homogenized in NOS assay buffer and further processed following the Nitric Oxide Synthase Activity Assay kit (Abcam, ab211083) protocol for measuring enzymatic activity of nitric oxide synthase (NOS) ...
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bioRxiv - Cancer Biology 2019Quote: ... Nuclear protein sample extract from washed cell pellets was carried out according to the manufactures protocol using cell fraction kit – standard (Abcam, ab109719). Nuclear pellets were resuspended in 100mM ammonium bicarbonate and lysed by sonication at 26 joules ...
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bioRxiv - Cancer Biology 2020Quote: ... IL-6 levels in TCM of met-low and met-high melanoma and breast carcinoma tumors were quantified using a specific enzyme-linked immunosorbent assay (ELISA) kit (ab46100, Abcam, US). The ELISA experiments were performed with 5-8 mice per group ...
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bioRxiv - Neuroscience 2021Quote: ... NT-3 was measured by standard ELISA according to manufacturer’s instructions (ab100615 – Neurotrophin 3 human ELISA Kit, abcam, Cambridge, United Kingdom).
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... and deparaffinized in serial baths of xylene and ethanol followed by staining using Mayer hematoxylin and 0.2% eosin (Histolab Products AB, Askim, Sweden) or picrosirius red staining kit (Abcam, Cambridge, UK). The stained slides were imaged using an Aperio CS2 image capture device.
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bioRxiv - Cancer Biology 2020Quote: ... Cells were incubated with CM for 24 or 48h after which cells were stained to detect DNA fragmentation in apoptosis using the BrdU-Red DNA (TUNEL) kit (Abcam, ab66110) following the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2021Quote: Histological analyses of the NAFLD tissues were performed using the H&E Stain Kit (Hematoxylin and Eosin) (ab245880; Abcam, Cambridge, UK) and the Oil Red O Stain Kit (Lipid Stain ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... and deparaffinized in serial baths of xylene and ethanol followed by staining using Mayer hematoxylin and 0.2% eosin (Histolab) or picrosirius red staining kit (Abcam, Cambridge, UK). The stained slides were imaged using an Olympus BX60F microscope with an SC50 camera.
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bioRxiv - Cell Biology 2021Quote: The relative contents of endogenous sphingomyelins in WT and SIRT1 KO mESCs were analyzed using a commercially available Sphingomyelin Assay Kit (Abcam ab133118) per manufacturer’s instruction.
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bioRxiv - Cell Biology 2022Quote: ... Sorted satellite cells were treated with UV radiation at different dosages and the comet assay was operated immediately after irradiation through a Comet Assay Kit (Abcam, ab238544) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2022Quote: Grown BEC-organoids were treated with the FA-mix for 4 days and triglyceride levels were measured with a Triglyceride kit (Abcam, ab65336) following the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2022Quote: ... protein was extracted from the 8 cultures using the lysis buffer from the GFP Fluorescent ELISA Kit (ab229403) (Abcam, Cambridge, UK) that was supplemented with Halt Protease Inhibitor Cocktail (Thermo Fisher Scientific ...
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bioRxiv - Evolutionary Biology 2022Quote: One hundred µl of the cell culture was transferred into a 96-well microplate provided by the In-Cell ELISA kit (Abcam, ab111541), and treated following the manufacturer’s recommendations ...
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bioRxiv - Microbiology 2022Quote: ... Monoclonal α-GPEET and α-EP IgG were conjugated with Allophycocyanin (APC) fluorescence molecule by APC Conjugation Kit – Lightning Link (Abcam). Cells were stained with α-GPEET or α-EP or both α-GPEET and α-EP to detect the loss of GPEET and EP as the indicator of the presence of non-PCF cells in the induced culture ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... The final concentration and integrity of eGFP-WNT-3A in the conditioned medium were determined using both ELISA (GFP ELISA® kit, #ab171581, Abcam) and SDS-PAGE/Western Blot analysis ...
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bioRxiv - Neuroscience 2023Quote: Conditioned media from the somatic side of the same MFCs used for the ELISA was analysed with the Cytotoxicity LDH assay kit (Abcam #ab65393) following manufacturer’s instructions ...
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bioRxiv - Physiology 2022Quote: ... Immunohistochemical staining for MMP13 was performed utilizing the Innovex Universal Animal IHC Kit and a polyclonal rabbit anti-mouse MMP13 antibody (Abcam ab39012). Briefly ...
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bioRxiv - Genetics 2022Quote: ... The mixture was centrifuged at 10000g for 5 min and 5μl of the supernatant was used to measure fumarate levels per instruction from the commercial fumarate kit (Abcam, Cambridge, UK).
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bioRxiv - Microbiology 2022Quote: ... and nuclear DNA-encoded succinate dehydrogenase A (SDH-A) using the MitoBiogenesis In-Cell Enzyme-linked immunosorbent assay (ELISA) Kit (Colorimetric, Abcam, ab110217) following the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2022Quote: ELISA kits were used to quantitate the mouse plasma levels of YKL-40 (1:40 dilution, #AB238262; ABCAM, Cambridge, MA, USA), IL-6 (1:4-1:2 dilution ...
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bioRxiv - Physiology 2022Quote: RalGDS RBD Agarose beads were used to selectively pull-down the active form of Rap1 (Rap-GTP) from isolated cardiac mitochondria (Rap1 Activation Assay Kit, Abcam, ab212011). Samples of mitochondria ...
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bioRxiv - Cancer Biology 2022Quote: ... cells were incubated for 1 hour at room temperature with PSR staining solution before rinsing twice in Acetic Acid solution (Picrosirius Red Stain Kit, Abcam, ab150681) and imaging ...
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bioRxiv - Cell Biology 2023Quote: Detection of cytoplasmatic and mitochondrial ROS levels was performed by means of a commercial DCFDA / H2DCFDA - Cellular ROS Assay Kit (abcam, ab113851) and MitoSOX™ Red Mitochondrial Superoxide Indicator (Invitrogen ...
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bioRxiv - Microbiology 2023Quote: ... The preparation of samples and lactate level measurement procedure were followed as specified in the Lactate Assay Kit (Colorimetric) (ABCAM - ab65331) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... The measurement of Cathepsin L activity was performed following the instructions and the protocol of Magic Red Cathepsin Assays Kit (Abcam, ab270774). Photos were taken using a Floid Cell Imaging Station (Thermo Fisher Scientist) ...
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bioRxiv - Neuroscience 2023Quote: ... 300μg of protein was used for immunoprecipitation assay using immunoprecipitation kit containing Dynabeads-coupled protein G (magnetic beads) and human anti-MMP9 (1:700) (Abcam, ab38898). Briefly the protein was mixed with anti-MMP9 antibody and incubated overnight at 4°C ...
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bioRxiv - Molecular Biology 2023Quote: LAL activity was determined in control and VPS35-deficient Hepa1-6 cells using the LysoLive™ Lysosomal Acid Lipase Assay Kit (ab253380, Abcam) according to the manufacturer’s instructions for adherent cells ...
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bioRxiv - Cancer Biology 2023Quote: ... Immunohistochemical SP method (according to Fuzhou Maishin immunohistochemical ultrasensitive mouse/rabbit SP IHC ready-to-use kit) was performed to detect the expression of CK2A1 (Abcam 76040), CK2A2 (Invitrogen PA5-109601) ...
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bioRxiv - Cancer Biology 2023Quote: ... IHC labeling for c-Myc was performed using the DISCOVERY HQ+ Amp kit with primary antibody against c-MYC (1:600, Abcam, ab32072). IHC for EdU and MYC on cells grown on chamber slides was performed by following the protocol described before (48) ...
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bioRxiv - Immunology 2023Quote: ... Thymocytes were isolated from untreated and TBI-treated mice and intracellular pyruvate and lactate levels were measured by absorbance using Pyruvate Assay kit (Abcam, ab65342) or Lactate-Glo™ Assay (Promega ...
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bioRxiv - Genetics 2022Quote: ATPase activity of cohesin was measured using EnzChek Phosphate Assay Kit with purified recombinant proteins depleted of free phosphate using Inorganic Phosphate Binding Resin (Abcam: ab270547). Reactions were assembled with10nM cohesin ...
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bioRxiv - Cell Biology 2023Quote: The concentration of VEGF-A in human lung cancer cell lines was determined by a VEGF Human ELISA kit (Abcam, #ab100662). ELISA assays were performed according to the manufacturer manual ...
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bioRxiv - Physiology 2023Quote: ... Immunoprecipitation was accomplished using plate based-beads in the EpiQuik Kit along with HNF4α antibodies (Novus NB100-1783 and Abcam ab181604) to validate enrichment at QPRT ...
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bioRxiv - Neuroscience 2023Quote: ... the cryosections of human brain were mounted on a Polyethylene naphthalate (PEN) Membrane Slide (Zeiss, 415190-9041-000) and then stained with spike protein antibodies using the IHC kit (Abcam, ab210062). After further processing ...
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bioRxiv - Cell Biology 2023Quote: Senescence-associated β-galactosidase (SA-β-gal) was used to detect MSCs senescence using SA-β-gal staining kit (Abcam, AB65351) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... Samples were pre-cleared using the beads provided in the kit and incubated overnight at 4°C with 5 µg of commercially available antibodies against lamin A/C (Abcam, #ab108595), phospho-Ser301 lamin A/C (Affinity ...
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bioRxiv - Cancer Biology 2023Quote: ... Samples were diluted 1:400 in Milli-Q water and lactate concentration was measured using a Lactate assay kit (Abcam, ab65330) according to the manufacturer’s protocol (using 1.5 µL of Enzyme Mix per well) ...
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bioRxiv - Physiology 2023Quote: The same plasma samples were diluted 10-fold and corticosterone (CORT) was measured using a commercial competitive ELISA kit (Abcam, AB108821). The assay had a sensitivity of 0.30 ng/ml in a standard range of 0.39 ng/ml to 100 ng/ml ...
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bioRxiv - Cell Biology 2023Quote: ... These different recombinants were used to measure phospholipase A2 (PLA2) activity following procedures described in the kit manual (Abcam, Cambridge, UK), in the presence of ethanol or P4 (10-5M) ...
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bioRxiv - Neuroscience 2023Quote: Measurement of the oxygen consumption rate (OCR) in hiPSC-derived neurons was performed with the fluorescence-based Extracellular O2 Consumption Assay Kit (Abcam, ab197243) as described previously (Castelo Rueda et al. ...
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bioRxiv - Genetics 2023Quote: ... slides were stained for goblet cells and mucins using an Alcian Blue dye and Nuclear Red Fast counterstain kit (Abcam; ab150662). Quantification of the percent area of Alcian blue staining ...
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bioRxiv - Biochemistry 2023Quote: ... The lipid fraction from 1 million cells per sample was extracted after which total cholesterol was assayed using the Cholesterol/Cholesteryl Ester Quantitation Assay kit (Abcam, ab65359) according to the manufacturer’s recommendations ...
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bioRxiv - Neuroscience 2023Quote: ... and cathepsin D (CatD) endo-peptidase activity was determined using commercial assay kits (Cathepsin B: ab65300, Abcam; Cathepsin D: ab65302, Abcam). Samples were homogenized in Lysis buffer as per the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... Infected cell lines were incubated at 37°C in a carbogen environment for two hours prior to conducting cytotoxicity assays using a lactate dehydrogenase (LDH) kit (Abcam, UK) as previously described(48) ...
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bioRxiv - Immunology 2024Quote: ... was performed by flow cytometry and triglyceride release assay was determined in supernatant after low-speed centrifugation (300 g, 10 min) using Triglyceride Assay kit (Abcam ab65336). GMPs were collected in a supernatant of granulocytes (3-5 x 106/ml ...
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bioRxiv - Cancer Biology 2023Quote: To quantitatively measure intracellular reactive oxygen species in live cell samples we have used cell permeant reagent 2’,7’–dichlorofluorescin diacetate (H2DCFDA) from Cellular ROS Assay Kit (Abcam, ab113851) as per the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: For measuring cellular iron in Fe2+ and Fe3+ states as well as total iron content we have a used a colorimetric Iron Assay Kit system (Abcam, ab83366) in stepwise manner as described in the manufacture’s protocol ...
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bioRxiv - Cancer Biology 2023Quote: ... The pellet was centrifuged once again and re-suspended in nuclear extraction buffer from the kit to generate the nuclear fraction and samples used for the NF-κB p65 transcription ELISA based assay (Abcam, ab133112) according to the manufacturer’s instructions.