Labshake search
Citations for abcam :
1951 - 2000 of 2129 citations for hsa mir 483 5p Real time RT PCR Detection Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: ... mouse PGE2 ELISA Kit (ab287802and Cytokine & Chemokine 36- Plex Mouse ProcartaPlexTM Panel 1A (EPX360-26092-901) for Luminex assay were obtained from Abcam and Thermofisher respectively ...
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bioRxiv - Physiology 2023Quote: ... Ferric Reducing Antioxidant Power (FRAP) assay of maternal plasma was done according to manufacturer instructions (FRAP Assay Kit, colorimetric, ab234626, Abcam).
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bioRxiv - Molecular Biology 2023Quote: ... Separation of U2OS cellular compartments was performed according to the manufacturer’s instructions with a Standard Cell Fractionation kit (abcam # ab109719). Two different markers were used to assess the purity of each fraction ...
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bioRxiv - Microbiology 2023Quote: Gamma-irradiated infected and non-infected tissue specimens were homogenized on ice using Dounce Glass Manual Tissue Grinder (Etcon) following the Plasma Membrane Protein Extraction Kit protocol (Cat# ab65400, Abcam). Total protein concentration was quantified using Pierce™ BCA Protein Assay Kit (Thermo Fisher) ...
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bioRxiv - Cancer Biology 2023Quote: ... and the cells were lysed in 80 μL of ice-cold buffer from Glutathione Assay Kit (catalog no: ab239709, Abcam). The lysed cells were kept on ice for 10 minutes followed by mixing thoroughly with 20 μL of 5% SSA (sulfosalicylic acid ...
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bioRxiv - Cell Biology 2023Quote: APOB protein levels in RPE and retinal lysates were measured using an Apo B ELISA Kit (ab230932, Abcam Cambridge, UK). Briefly ...
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bioRxiv - Cancer Biology 2023Quote: ... Pyruvate concentration in the centrifuged extracellular medium samples (5 min 18000 g, 4°C) was measured in microplates using Pyruvate Assay kit (Abcam) or as described in 36.
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bioRxiv - Neuroscience 2023Quote: ... Human temporal cortex samples were lysed in CB Cell Lysis Buffer from the Cathepsin B Activity Assay Kit (Abcam, ab65300). Mouse cortex samples were lysed in RIPA buffer (150 mm sodium chloride ...
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bioRxiv - Neuroscience 2023Quote: ... and cathepsin D (CatD) endo-peptidase activity was determined using commercial assay kits (Cathepsin B: ab65300, Abcam; Cathepsin D: ab65302, Abcam). Samples were homogenized in Lysis buffer as per the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... flash frozen in liquid nitrogen and stored at -80°C or further concentrated using a polyethylene glycol (PEG) virus precipitation kit (Abcam) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2024Quote: ... adult mouse cardiomyocyte cell line HL-1 or primary neonatal mouse cardiac fibroblasts (NMCF) or cardiomyocytes (NMCM) using the Histone extraction kit (Abcam) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: ... The amount of cholesterol in cells and supernatants was assessed using the cholesterol/cholesteryl ester assay quantification kit (ab65359, abcam) according to the manufacturer’s instructions.
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bioRxiv - Immunology 2024Quote: ... spent media was collected for each condition and lactate secretion was assayed in duplicate or triplicate using either Lactate Colorimetric/Fluorometric kit (abcam) or Lactate-Glo (Promega ...
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bioRxiv - Immunology 2024Quote: ... Cellular ROS was also detected using the DCFDA/H2DCFDA cellular ROS assay kit according to the manufacturer’s instructions (Abcam; ab113851). To analyze cell populations ex vivo ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... that occurred per minute of incubation with each substrate at 37°C by Aspartate Aminotransferase Activity Assay Kit (Abcam ab105135) and the Alanine Transaminase Activity Assay Kit (Abcam ab105134) ...
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bioRxiv - Microbiology 2024Quote: ... 5% CO2 for 24 h before supernatants were collected and LDH levels determined using an LDH-Cytotoxicity Assay Kit (abcam).
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bioRxiv - Cancer Biology 2024Quote: ... non-esterified fatty acids (NEFA) and glycerol were determined using enzymatic kits (Triglyceride SL, Sekisui Diagnostics; NEFA-HR, Wako; glycerol, ab65337 Abcam) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2024Quote: Free glycerol was measured in the infranatant fraction of the isolated adipocyte culture using a colorimetric assay kit (ab65337, Abcam) following the manufacturer’s instructions.
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bioRxiv - Physiology 2024Quote: ... Mitochondrial membrane potential in C2C12 myotubes was measured in a fluorescence plate reader (535 nm/580 nm (ex./em, respectively) with 50 nM TMRE and following the TMRE Mitochondrial Membrane Potential Assay Kit (ab113852, Abcam) manufacturer’s instructions.
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bioRxiv - Microbiology 2024Quote: ... Pan-flavivirus envelope protein antibody 4G2 was conjugated to APC using Lightning-Link® APC Conjugation Kit (abcam, Cat# ab201807). Cells were then blocked with Human TruStain FcX in FACS on ice for 10 minutes followed by staining with 4G2-APC in 1x Flow Cytometry Perm Buffer on ice for 20 minutes ...
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bioRxiv - Cell Biology 2024Quote: EA.hy926 cells or EZH2 WT/mutant overexpressing HEK-293 treated with NO donor SNP (500 µM) for 2 hours were processed with the help of a Biotin Switch Assay kit (Abcam) using the standard manufacturer protocol ...
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bioRxiv - Microbiology 2023Quote: ... the resulting supernatant fractions were boiled for 10 min to inactivate enzymatic activity and their glycogen content measured using a Glycogen Assay Kit (ab65620, Abcam). Briefly ...
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bioRxiv - Neuroscience 2023Quote: ... cellular lysates were incubated for 1h with LND or vehicle with was measured using a hexokinase assay kit (Abcam, ab211103) according to manufacturer instructions.
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bioRxiv - Molecular Biology 2024Quote: AP-sites were tested in U2OS cells 48 h after transfection with control and UNG siRNA by a DNA damage assay kit (ab211154, Abcam) according to the manufacturer’s instructions.
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bioRxiv - Molecular Biology 2024Quote: ... Samples were centrifuged at 1,000 g for 30 minutes before proceeding to ELISA (Human FAS Ligand ELISA Kit - CD95L –Abcam-ab100515).
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bioRxiv - Molecular Biology 2024Quote: Metabolic activity within periosteal MSC was quantified using a Cell Counting Kit 8 assay (WST-8 / CCK8; Abcam, Cambridge, UK). Water soluble tetrazolium salts are used to quantify cell viability through the production of formazan dye similar to that of an MTT assay ...
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bioRxiv - Bioengineering 2024Quote: ... ELISA was performed on all serum samples after day 20 to measure the serum TTR level using Mouse Prealbumin ELISA kit (Abcam).
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bioRxiv - Cancer Biology 2024Quote: ChIP experiments were performed in duplicate for WT and MT cells (clones MT1 and MT2) using the High Sensitivity ChIP Kit (Abcam). 5x105 cells were used as input material for each immunoprecipitation ...
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bioRxiv - Cancer Biology 2024Quote: Surface-expressed EREG molecules/cell were quantified by labeling H231 with phycoerythrin (PE) with the Lightning-Link PE conjugation kit (Abcam) and BD QuantiBRITE PE ...
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bioRxiv - Biochemistry 2024Quote: ... 2 ml of cells were harvested by centrifugation and treated according to the standard protocol (TUNEL Assay Kit – FITC, ab66108, Abcam). Measurement of FITC fluorescence was performed with CytoFLEX cytometer (Beckman Coulter ...
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bioRxiv - Cell Biology 2019Quote: The TC and free cholesterol content of hLECs were measured colorimetrically using a Cholesterol assay kit according to the manufacturer’s protocol (#ab65359; Abcam, Cambridge, UK). Harvest the amount of hLECs necessary for each assay (initial recommend = 1 x 107 cells) ...
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bioRxiv - Immunology 2021Quote: ... Intracellular HO-1 staining for human and mouse was performed using the Invitrogen FIX & PERM Kit (GAS003) using HO-1 PE at 1:100 dilution (Abcam, ab83214) and PE IgG2b isotype (Abcam ...
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bioRxiv - Cell Biology 2022Quote: ... NAD+/NADH ratio and the concentration of total NAD+ and NADH was determined by NAD/NADH fluorometric assay kit from Abcam (ab176723) following manufacture’s protocol ...
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bioRxiv - Neuroscience 2021Quote: Levels of anti-inflammatory cytokine Transforming growth factor-β1 (TGF-β1) in supernatants were measured by an ELISA kit (ab119558, abcam, UK), following manufacturer’s instructions ...
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bioRxiv - Neuroscience 2020Quote: ... Oil Red O (ORO) staining was performed on muscle cryosections (10μm) to visualize neutral lipids using an ORO kit (Abcam, Cambridge, GBR). Briefly ...
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bioRxiv - Bioengineering 2020Quote: Quantification of proteins via C-terminal GFP tags was performed using a GFP quantification kit from AbCam (Cambridge, UK, product # ab171581) according to manufacturer’s instructions ...
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bioRxiv - Neuroscience 2020Quote: ... The general activity of MMPs in iPSC-astrocyte supernatants was determined using the MMP Activity Assay Kit (Abcam, Cat. no.: ab112147) following the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2020Quote: ... ChIP for RNA polymerase II was performed according the iDeal ChIP-Seq Kit instructions using the anti-Pol2 antibody (Abcam, ab5408). Immunoprecipitated DNA was bisulfite converted using the EZ DNA Methylation Gold Kit (Zymo) ...
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bioRxiv - Bioengineering 2021Quote: ... Twenty microliters of reagent was added directly to each well plate following the instructions of the MTS Cell Proliferation Assay Kit (colorimetric ab197010, abcam®). The culture plate was incubated for 3 h at 37°C in a humidified atmosphere containing 5% CO2 ...
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bioRxiv - Cell Biology 2021Quote: The presence of S protein in COVID-19 patients’ serum was evaluated using the COVID-19 Spike Protein ELISA Kit from Abcam (ab274342), according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: Quantification of the amount of human ACE2 in the different EV and fractions was done using the human ACE2 ELISA kit (Abcam, ab235649) following manufacturer’s instructions.
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bioRxiv - Plant Biology 2021Quote: ... 1 mM dithiothreitol (DTT) and 1% (v/v) protease inhibitor cocktail and then used for protein quantification with BCA protein quantitative Kit (Abcam ab6789). The protein sample (200μg amounts ...
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bioRxiv - Microbiology 2021Quote: ... Serum concentrations of immunoglobulin A (IgA) and immuno-globulin M (IgM) were measured using enzyme-linked immunosorbent assays kits (ELISA; Abcam, USA), according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2021Quote: The activity of mitochondria Complex I was measured in peeled murine esophageal epithelium using Complex I Enzyme Activity Assay kit (Abcam, ab109721) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2020Quote: ... cells were incubated with filipin III (1:100) in PBS for 2 h as described in the manufacturer’s protocol of the cholesterol assay kit (Abcam Biochemicals, ab133116). Filipin III was also added in all following incubation steps with the primary and secondary antibody to enable proper staining ...
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bioRxiv - Cell Biology 2021Quote: Cathepsin B activity was assessed in mouse primary neuronal samples and mouse brain hippocampus using Fluorometric Cathepsin B activity assay kit (abcam, 65300) following the manufacturer’s protocol ...
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bioRxiv - Microbiology 2022Quote: ... solani IPO 2222 WT strain and ΦD5□resistant Tn5 mutants were isolated using a Lipopolysaccharide Extraction Kit (Abcam, Symbios, Gdansk, Poland) with a modified protocol (Bartnik et al. ...
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bioRxiv - Immunology 2022Quote: ... Alanine liberated was measured as increasing fluorescence signal (Resorufin, Ex/Em: 535/587 nm) recorded at 25 °C using an L-alanine assay kit (Abcam, ab83394) at 25 °C according to manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: ... and CTLA-4 Ig (Abatacept) were generated using the APC Conjugation Kit - Lightning-Link® according to manufacturer’s instructions (Abcam, ab201807). APC conjugated anti-PD-L1 antibodies were procured from Biolegend (clones MIH1 ...
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bioRxiv - Molecular Biology 2022Quote: ... Slides were gently washed in phosphate buffered saline (PBS) prior to blocking with the blocking reagent from the mouse-on-mouse polymer IHC Kit (Abcam ab269452). The tissues were then incubated overnight with the primary antibody at 4°C ...