Labshake search
Citations for abcam :
1901 - 1950 of 2129 citations for hsa mir 483 5p Real time RT PCR Detection Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: Glyceraldehyde 3 Phosphate Dehydrogenase Activity was measured by using Glyceraldehyde 3 Phosphate Dehydrogenase Activity Assay kit (Colorimetric) (Abcam®, #ab204732) according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2021Quote: ... 500 µL of supernatant fluid was added to 25 µL of pre-incubated protein G magnetic beads (ChIP-Adembeads ChIP-Adem-Kit, Ademtech SA) with 5 µg of anti-V5 rabbit polyclonal antibody (ab9116, Abcam) in IP buffer (50 mM HEPES-KOH pH 7.5 ...
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bioRxiv - Immunology 2021Quote: ... the induction of proliferation of naïve CD8+ T cells was assessed by BrdU Cell proliferation ELISA kit (Abcam, Cambridge, UK) according to the protocol of the manufacturer after 72 hours of co-culture ...
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bioRxiv - Genomics 2021Quote: Serum 17 beta-estradiol was measured using a randomized plate layout with 3 replicates per sample in female baboons on the LS and HS diets with the 17 beta Estradiol ELISA Kit (Abcam); human female serum (age 28 years ...
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bioRxiv - Neuroscience 2021Quote: The brain ATP level was measured through an ATP Assay Kit according to the manufacturer’s instructions (Abcam, Catalog No. ab83355)10 ...
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bioRxiv - Neuroscience 2021Quote: Plasma samples were used to determine triglycerides levels with the TG assay kit for quantification (Abcam, ab65336; Cambridge, MA, USA) as previously described (Kalyan-Masih et al. ...
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bioRxiv - Cell Biology 2022Quote: Filipin III stocks were made in ethanol and stored in aliquots at −80 °C according to kit instructions (Abcam, ab133116). When ready for use ...
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bioRxiv - Cancer Biology 2022Quote: ... 10 µg of nuclear extract were used for the TP53 functional assay that was conducted according to the manufacturer’s instructions with the calorimetric p53 Transcription Factor Assay Kit (Abcam, ab207225). In short ...
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bioRxiv - Bioengineering 2022Quote: The XCL-1 measurement in mouse blood plasma samples was performed by using Mouse XCL- 1 SimpleStep ELISA kit (Abcam). The assay was performed as per manufacturer guidelines ...
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bioRxiv - Cancer Biology 2022Quote: Cell viability of SHANK3-silenced PANC-1 cells treated with ERK and MEK inhibitors were measured using Cell Counting Kit-8 (WST-8 / CCK-8; Cat. no. ab228554, Abcam). Cells were silenced for 24 h as described above and then seeded on a 96-well plate with full medium containing DMSO (control) ...
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bioRxiv - Evolutionary Biology 2022Quote: ... an aliquot of the reaction was used to determine the amount of synthesized ATP using ATP Assay Kit (Colorimetric/Fluorometric) (Abcam). The assay was performed at 37 °C ...
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bioRxiv - Genomics 2022Quote: We determined the cytomegalovirus (CMV) serostatus of AFB (n = 80) and EUB (n = 80) donors with a human anti-IgG CMV ELISA kit (Abcam) on plasma samples ...
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bioRxiv - Microbiology 2022Quote: Membrane fluidity was measured as previously described (136) using a membrane fluidity kit that quantifies the fluorescence of a lipophilic dye (Pyrenedecanoic acid) (Abcam). Pyrene decanoic acid exists in monomeric and dimeric states within membranes ...
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bioRxiv - Systems Biology 2022Quote: ... Protein Assay Kit (Thermo-Fisher) and the activation of the c-Jun transcription factor was assayed by c-Jun Transcription Factor Assay Kit (Abcam) according to the manufacturer’s instruction ...
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bioRxiv - Plant Biology 2022Quote: ... 0.5 µm) were prepared and stained with 1% (w/v) toluidine blue and the Periodic Acid Schiff kit (ab150680, Abcam) as described in Hawkins et al ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... The virus titer was quantified by an ELISA assay against the lentiviral envelope protein p24 (HIV1 p24 ELISA Kit, Abcam). The Cas9 expressing MEF cells were infected in presence of 8 µg/ml Polybrene with the lentiviral particles at a multiplicity of infection of 0,3 to avoid multiple gene knock outs in one cell ...
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bioRxiv - Neuroscience 2022Quote: Human serum levels of LBP were assayed using the Human LBP ELISA kit standard sandwich enzyme-linked immunosorbent technology and following the manufacturer’s protocol (Abcam, ab213805). Briefly ...
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bioRxiv - Neuroscience 2023Quote: ... Cell viability was assessed after 72 hours using a 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium (MTS) assay kit (Abcam, ab197010) according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2023Quote: Subcellular fractionation of HeLa cells transfected with the ZC3H11A-Flag expressing plasmids (5μg plasmid/100mm tissue culture plate) was done using Cell Fractionation Kit (Abcam, ab109719) according to the manufacturer’s instructions ...
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bioRxiv - Physiology 2022Quote: Quantitative cAMP measurements were carried out on isolated cardiac mitochondria (0.5 mg per sample) using cAMP Direct Immunoassay Kit (Fluorometric) (Abcam, ab138880). Mitochondria samples were treated with 0 or 10 or 15 mM [HCO3−] ...
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bioRxiv - Molecular Biology 2022Quote: ... A TET activity assay was also performed on nuclear extracts using a fluorometric commercial assay (TET Hydroxylase Activity Quantification Kit, catalog# ab156913; Abcam) and microplate fluorometer (Synergy H1 ...
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bioRxiv - Molecular Biology 2022Quote: A DNMT activity assay was performed on nuclear extracts using a commercial assay (Colorimetric DNMT Activity Quantification Kit, catalog# ab113467; Abcam) and microplate spectrophotometer (Synergy H1 ...
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bioRxiv - Developmental Biology 2022Quote: ATP synthase activity of wild-type and stat3 mutant zebrafish embryos was measured using the ATP Synthase Specific Activity Microplate Assay Kit (Abcam) following the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2023Quote: The activity of lysyl oxidase (LOX) in CM samples (n = 27) was measured using fluorometric commercial assay (Lysyl Oxidase Activity Assay Kit, Abcam) following the manufacturer’s protocol ...
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bioRxiv - Microbiology 2023Quote: ... Caspase activation was assayed with the Caspase-3 and Caspase-1 colorimetric assay kits from abcam (ab39401 and ab273268, respectively). At harvest ...
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α-synuclein expression in response to bacterial ligands and metabolites in gut enteroendocrine cellsbioRxiv - Neuroscience 2023Quote: The amount of α-synuclein in the culture medium was measured using a mouse α-synuclein ELISA kit (Abcam ab282865) according to the manufacturer’s instructions.
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bioRxiv - Neuroscience 2023Quote: ... and native histones that contain the modification and were purified from HEK293T cells using a Histone Extraction Kit (Abcam ab113476). Protein concentration was determined using the Pierce BCA assay ...
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bioRxiv - Molecular Biology 2023Quote: ... gel followed by either silver staining with Pierce Silver Stain Kit (Thermo) or Western blotting against anti-NOP58 (#ab236724, Abcam) and anti-GAPDH (#5174s ...
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bioRxiv - Neuroscience 2023Quote: ... were labeled with the fluorescent dye Alexa Fluor 647 according to the manufacturer’s protocol (Alexa Fluor 647 conjugation kit lightning link, Abcam, ab269823). Briefly ...
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bioRxiv - Bioengineering 2023Quote: ... Blood was then centrifuged at 1500 x g for 10 minutes and plasma was collected and analyzed with a TAT ELISA kit (Abcam) according to the manufacturer’s instructions.
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bioRxiv - Microbiology 2023Quote: ROS production of the donor strain was measured by using a 2’,7’–dichlorofluorescein diacetate (DCFDA) kit (abcam®, UK), following the manufacturer’s protocol ...
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bioRxiv - Microbiology 2023Quote: ... Reactions were transferred to a 96-well plate and the amount of inorganic phosphate released from the hydrolysis of NTP was determined using Phosphate Assay Kit PiColorLock (Abcam) according to protocol by the manufacturer ...
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bioRxiv - Cancer Biology 2023Quote: ... the quantification of changes in mitochondrial membrane potential was determined following the protocol of the TMRE-Mitochondrial Membrane Potential Assay Kit (Abcam). Briefly ...
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bioRxiv - Neuroscience 2023Quote: ... and cathepsin D (CatD) endo-peptidase activity was determined using commercial assay kits (Cathepsin B: ab65300, Abcam; Cathepsin D: ab65302, Abcam). Samples were homogenized in Lysis buffer as per the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... PSA levels were quantified both in the conditioned media and in the mouse sera using the PSA ELISA kit (Abcam), according to the manufacturer’s instructions.
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bioRxiv - Neuroscience 2023Quote: ... Total nitrite/nitrate was measured as a proxy for nitric oxide concentration using the Nitric Oxide Assay Kit (Abcam, ab65327), with 5 hours of incubation with the enzyme cofactor to permit more than 99% conversion of nitric oxide to nitrite.
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bioRxiv - Molecular Biology 2023Quote: ... cell viability was evaluated at the end of incubations by a dehydrogenase activity assay (Cell Counting Kit 8, Abcam #ab228554), and expressed relative to the solvent control.
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bioRxiv - Microbiology 2023Quote: ... A bacterial culture containing about 109 exponentially growing Leptospira was used to determine the catalase activity using the Catalase Activity Assay Kit (Abcam), according to manufacturer’s instructions.
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bioRxiv - Neuroscience 2023Quote: ... Human temporal cortex samples were lysed in CB Cell Lysis Buffer from the Cathepsin B Activity Assay Kit (Abcam, ab65300). Mouse cortex samples were lysed in RIPA buffer (150 mm sodium chloride ...
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bioRxiv - Immunology 2023Quote: ... supernatants from the cocultures were tested in a hemoglobin release assay at a dilution of 1:1600 (Human Hemoglobin ELISA kit, Abcam) according to manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: C3 or C5 levels in feces or serum of mice were measured with a Mouse C3 ELISA kit (Abcam, ab157711) or a Mouse C5 ELISA kit (Abcam ...
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bioRxiv - Cell Biology 2022Quote: Cell culture supernatant of differentiated Hoxb8 macrophages was collected and the amount of soluble Galectin-3 was quantitatively assessed using the Galectin-3 Mouse SimpleStep enzyme-linked immuno-sorbent assay (ELISA) kit (Abcam) according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2022Quote: ... Anti-1D4 Ab and anti-OLLAS Ab were conjugated to PE using an Ab conjugation kit from Abcam (cat. 102918) according to the manufacturer’s protocols ...
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bioRxiv - Cell Biology 2023Quote: The Dihydroethidium (DHE) assay kit for the measurement of intracellular superoxide and hydrogen peroxide was purchased from Abcam (Cat#ab236206). The MitoSOXTM for the measurement of mitochondria superoxide was purchased from ThermoFisher Scientific (Cat#M36008) ...
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bioRxiv - Developmental Biology 2023Quote: EdU (5-ethynyl-2′-deoxyuridine) labeling assay was performed according to the instruction of EdU Assay / EdU Staining Proliferation Kit (ab219801, Abcam). Proliferating cells were labeled by injecting 10mg/kg BW EdU to mice intraperitoneally 2 hours before sacrifice ...
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bioRxiv - Cancer Biology 2023Quote: The 5-ethynyl-2’-deoxyuridine (EdU) incorporation assay was performed using the EdU Proliferation Assay Kit (iFluor 488) (ab219801, Abcam). Cells were seeded onto coverslips and incubated with 10 μM EdU for 4 h at 37°C in a humidified atmosphere with 5% CO2 ...
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bioRxiv - Cancer Biology 2023Quote: Changes in mitochondrial membrane potential produced by I3C and BNF in the five cancer cell lines was determined with the tetramethylrhodamine ethyl ester (TMRE) Mitochondrial Membrane Potential Assay Kit (ab113852, Abcam) following the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2023Quote: ... Protein carbonylation was detected by preparing skeletal muscle lysates from approximately 50 mg of muscle by homogenizing in lysis buffer provided by the Protein Carbonyl Content Assay Kit (Abcam). The resulting supernatants were subjected to western blotting following the manufacturer’s instructions.
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bioRxiv - Physiology 2023Quote: ... Ferric Reducing Antioxidant Power (FRAP) assay of maternal plasma was done according to manufacturer instructions (FRAP Assay Kit, colorimetric, ab234626, Abcam).
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bioRxiv - Physiology 2023Quote: ... cells were equilibrated to room temperature and stained with PI for 30 minutes at 37°C using Propidium Iodide Flow Cytometry Kit (ab139418; Abcam) according to the manufacturer’s protocol and incubated at 37°C in the dark for 30 minutes ...