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Citations for abcam :
2001 - 2050 of 3578 citations for 2H Pyrazolo 4 3 c pyridine 3 chloro 4 5 6 7 tetrahydro since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2022Quote: ... and IL-6 (Abcam, 46100) according to manufacturer’s protocol.
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bioRxiv - Physiology 2023Quote: ... 6-phosphogluconate dehydrogenase (Abcam, ab129199), transaldolase 1 (ThermoFisher Scientific ...
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bioRxiv - Bioengineering 2024Quote: ... anti-IL-6 (ab229381, Abcam), and anti-IL-1β (ab9722 ...
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bioRxiv - Cell Biology 2020Quote: ... and heated to 95 °C for 5 min (no heating of the samples used for the total oxphos (abcam, ab110413) blots) ...
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bioRxiv - Neuroscience 2024Quote: ... Sections were then incubated overnight at 4⁰C on a plate rocker in a 5% NGS/0.1% PBS-Triton solution containing rabbit anti-cFos antibody (Abcam, ab190289) at 1:500 dilution ...
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bioRxiv - Immunology 2024Quote: ... ∼50 µg of lysate material was treated with the reducing agent TCEP at a final concentration of 5 mM and heated to 60 °C for 30 minutes before being loaded onto a FASP protein digestion column (Abcam)75 ...
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bioRxiv - Molecular Biology 2020Quote: ... Fragmented DNA was re-purified using Phenol: Chloroform extraction and 4 µg of DNA was immunoprecipitated using antibodies specific against 5mC (Abcam), dsDNA (Abcam ...
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bioRxiv - Cell Biology 2020Quote: ... Cells attached on coverslips were fixed in 4% (w/v) formaldehyde dissolved in PBS and immunocytochemistry (ICC) was performed according to a general ICC protocol (Abcam). Permeabilization of cells was done by incubating in PBS containing 0.1% (v/v ...
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bioRxiv - Neuroscience 2021Quote: Larvae were fixed in 4% paraformaldehyde with regions of interest labeled using the following primary antibodies: goat anti-GFP (1:200; Abcam), rabbit anti-serotonin (1:200 ...
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bioRxiv - Neuroscience 2020Quote: ... the cultures were fixed for 1h at room temperature in 4% paraformaldehyde and stained with mouse anti-neurofilament (RMO 270; Zymed) and rabbit anti-myc antibodies (Abcam). The number of neurofilament-positive cells was counted in 16 randomly selected frames (0.4 mm2) ...
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bioRxiv - Biochemistry 2021Quote: ... The immunoprecipitation of HA-tag proteins was performed overnight in a total volume of 600 μl with 4 μg of HA-antibody (ab9110, Abcam) except the 5% of samples taken as input ...
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bioRxiv - Cell Biology 2021Quote: ... Non-specific binding was blocked by incubating the membranes with 5% nonfat milk for 2 h at room temperature and the identity of proteins evaluated by incubation at 4 ℃ overnight with the following primary antibodies (Abcam): HSP70 (1:1000 ...
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bioRxiv - Cancer Biology 2020Quote: Cytosol and mitochondria were separated from A375 cells 2 hr or 4 hr after treatment using the Mitochondria/Cytosol Fractionation Kit (Abcam) following the manufacturer’s instructions.
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bioRxiv - Physiology 2022Quote: 10 µg of protein from 4-week-old adrenals were used for measurement with PKA kinase activity kit (ab139435, Abcam).
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bioRxiv - Molecular Biology 2022Quote: ... Chromatin fractions of 400 μl were taken for each immunoprecipitation reaction and incubated with 4 μl of anti-histone H3 antibodies (ab1791, Abcam) at 4°C overnight ...
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bioRxiv - Bioengineering 2022Quote: ... with PBST and stained for primary antibody at 1:200 in PBST for 1 hour at room temperature: mouse anti-alpha sarcomeric actinin (ab9465, Lot: GR3174517-4, Abcam), mouse anti-alpha smooth muscle actin ...
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bioRxiv - Molecular Biology 2021Quote: ... Sample buffer was added to the reaction and incubated at 37° for 20 minutes before SDS-PAGE and Western blot analysis using antibody MM13-4 (AbCAM). For each transfected construct ...
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bioRxiv - Cell Biology 2020Quote: ... The samples were washed with PBS and then incubated with biotinylated anti-mouse IgG secondary antibody (4 µg/ml, abcam) and Alexa Fluor 488 conjugated anti-rabbit IgG secondary antibody (1:250 ...
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bioRxiv - Physiology 2021Quote: ... Sections were blocked with 5% donkey serum in the presence of 0.1% Triton followed by overnight incubation with primary antisera at 4 degrees (pCREB, Cell Signaling; Glucagon, Abcam). After extensive washing ...
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bioRxiv - Evolutionary Biology 2021Quote: ... pCS2-lg-HAtag-WT and pCS2-lg-HAtag-MUT (2 μg) (Supplementary Fig. 4) were then transfected into the HEK293T cell line (ab255449, Abcam) separately ...
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bioRxiv - Neuroscience 2021Quote: ... peptide C-AGQERFH(pT)ITTSYYR (conjugated with either –KLH or -OVA) was used to inject 4 rabbits (New Zealand White rabbits) by Abcam. Rabbit bleeds/antisera were collected and screened by peptide ELISA assay and western blot ...
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bioRxiv - Neuroscience 2020Quote: ... 1 mm thick coronal sections were trimmed down to volumes of approximately 4 × 1 × 1 mm3 with a scalpel and incubated in Alexa Fluor-488 conjugated anti-NeuN (Abcam) for 120 hrs (Figure 2) ...
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bioRxiv - Neuroscience 2020Quote: ... followed by blocking in donkey serum for 30 min and incubating overnight at 4 □ with primary antibody (GPX4, rabbit, ab125066, 1:200, Abcam) in PBS ...
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bioRxiv - Developmental Biology 2023Quote: ... the samples were washed with several changes of PBS over minimum of 4 h at room temperature and mounted on microscopy slides with Anti-Fade Fluorescense Mounting Medium (Abcam).
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bioRxiv - Developmental Biology 2023Quote: ... the cells were incubated at 4□ for 30 min with an anti-Tra-1-85 antibody (Abcam, ab108308, 1:100) diluted in PBS including 1% BSA and then stained with a AlexaFluor 555-conjugated donkey anti-rabbit IgG secondary antibody (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2024Quote: ... at 1:10,000 followed by incubation with either secondary goat pAb to mouse IgG HRP (ab97040, Abcam, Lot # GR3219575-4) at 1:10,000 or secondary mouse antibody to rabbit IgG HRP (Santa Cruz Biotechnology ...
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bioRxiv - Cancer Biology 2023Quote: ... Where antibody staining was required coverslips were permeabilized with 0.2% Triton X-100 for 10 min and blocked in 4% BSA in PBS for 20 min followed by incubation with relevant antibodies (DHRS7 abcam at 1:1,000 dilution or rabbit lamin A antibodies [17] at 1:50 dilution for 1 hr at RT ...
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bioRxiv - Developmental Biology 2022Quote: ... peak incident power 105 W) and incubated overnight at 4° with magnetic beads conjugated to the H3K27ac antibody (Abcam, ab4729). The next day ...
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bioRxiv - Neuroscience 2022Quote: ... for 30 min followed by overnight incubation at 4° in primary antibodies, Aβ (Novus, NBP11 -97929) and NRN1 (Abcam, ab64186). Membranes were washed with 1×Tris-buffered saline containing 0.1% Tween 20 (TBS-T ...
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bioRxiv - Physiology 2022Quote: ... permeabilized and saturated for 30 minutes at room temperature in PBS with 0.01% Triton-X PBS and 4% donkey serum and incubated with chicken anti-GFAP (Abcam, ab4674) and rabbit anti-S100β (Dako ...
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bioRxiv - Immunology 2023Quote: The following primary antibodies were used for these experiments: rabbit anti-4 hydroxynonenal (HNE) antibody (10 µg/ml; ab46545, Abcam), rat anti-cd11b antibody (0.5 µg/ml ...
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bioRxiv - Immunology 2024Quote: Immunofluorescence staining of CCL17 and CCL22 in lymphoid tissues and atherosclerotic lesions was performed on 4% paraformaldehyde-fixed cryosections of mouse aortic roots using rabbit anti-CCL17 (1:200; abcam) or goat anti-CCL22 (1:200 ...
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bioRxiv - Immunology 2024Quote: ... 1*106 of purified human CD4+ T cells were treated with DOS-FITC for 4 hours and then stained with primary antibody anti-KDEL (1:100; ab12223; Abcam) and anti-sestrin 2 (1:100 ...
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bioRxiv - Physiology 2024Quote: ... 0.2% fish gelatin in 1 x PBS) BD Biosciences) and goat anti-rabbit 4-HNE (1:200 in blocking solution; Abcam) antibodies ...
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bioRxiv - Molecular Biology 2024Quote: ... Paraffin-embedded tissue sections of 4 µm thick from the distal colon were processed following the manufacturer’s protocol for the TUNEL assay kit (Abcam, #ab206386). Images of 10 randomly selected fields per slide were captured at 400X magnification ...
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bioRxiv - Bioengineering 2024Quote: ... followed by primary antibodies (IL-4 Abcam ab9811, 1:100; IL-13 Invitrogen PA5-102574, 1:100; TGF-β1 Abcam ab215715 ...
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bioRxiv - Bioengineering 2024Quote: Larval tissues were fixed in 4% paraformaldehyde/PBS + 0.1% Tween for 1 hour and stained overnight with a rabbit anti-GFP polyclonal antibody (Abcam 6556) and labelled using an appropriate Alexa Fluor 488 secondary dye (Molecular Probes).
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bioRxiv - Bioengineering 2024Quote: Levels of glucose and glutamine were determined via analysis of spent culture medium (n=4/group) using a Glucose Assay Kit and a Glutamine Assay Kit (Abcam), respectively ...
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bioRxiv - Cell Biology 2024Quote: ... including Xestospongin C (Xesto C, Abcam, Cat# ab120914), Cyclopiazonic acid (CPA ...
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bioRxiv - Biophysics 2022Quote: ... anti-mouse LA/C-C (131C3, ab8984, Abcam) anti-rabbit LA/C-rod (EP4520-16 ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Levels of IL-6 in supernatants were determined using human IL-6 ELISA kit (Abcam). The range for IL-6 detection was 7.8-500 pg/mL ...
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bioRxiv - Neuroscience 2021Quote: ... following which sections were blocked in 3% normal horse serum (NHS) for 30min before overnight incubation with Tyrosine hydroxylase (TH) (Abcam, ab112, 1:750) or IBA-1 (Wako ...
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bioRxiv - Neuroscience 2023Quote: ... slices were washed 3 times in 1x PBS for 10 minutes then incubated in blocking solution containing AlexaFlour488 conjugated secondary antibodies (Abcam, ab150077; 1:1000) for 2-4 hours at room temperature ...
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bioRxiv - Bioengineering 2023Quote: ... The slices were then incubated overnight at 4°C with primary antibody solutions (supplemented with 3% (v/v) Triton X-100 in 1× PBS) to target astrocytes (GFAP) and neuronal nuclei (NeuN) (Abcam Inc., Cambridge, UK) (Table 1) ...
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bioRxiv - Neuroscience 2022Quote: ... Sections were incubated at room temperature for ~3 hours in secondary antibody solution containing donkey anti-rabbit IgG H&L Texas Red (abcam, Cambridge, UK; ab7081) and donkey anti-goat IgG H&L Alexa Fluor 488 (abcam ...
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bioRxiv - Neuroscience 2022Quote: ... The same procedure was used for flat-mounts but primary incubation was for 3 days and secondary incubation for 1 day using anti-GFP 1:500 (Abcam, Cambridge, UK; Chk).
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bioRxiv - Microbiology 2023Quote: ... Primary antibody incubation was conducted for 3 hours at room temperature (1:2,000 α-N protein, Sino Biological 40143-R001; 1:500 α-nsp3, abcam ab283958) followed by a 1 hour ...
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bioRxiv - Cell Biology 2024Quote: ... gravid worms were placed in 1 drop of ddH2O containing 3% (v/v) pelleted OP50 and 100 μM bafilomycin A1 (Abcam, dissolved in DMSO) for 16 h at 20 ℃ ...
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bioRxiv - Cell Biology 2024Quote: The following antibodies were used for western blotting at the indicated dilutions: anti-CD63 (MBL, D263-3, 1:5000; Abcam, ab134045, 1:2000), anti–Flotilin-1 (BD Biosciences ...
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bioRxiv - Neuroscience 2024Quote: ... brain slices were incubated for 3 h at room temperature with a mix of secondary antibodies (ThermoFisher A-11039 or Abcam ab150158, 1:400) that had been previously centrifuged at 20000g for 10 minutes ...