Labshake search
Citations for abcam :
1801 - 1850 of 3578 citations for 2H Pyrazolo 4 3 c pyridine 3 chloro 4 5 6 7 tetrahydro since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2021Quote: ... rabbit anti-cytokeratin 7-Alexa555 (Abcam, ab209601), rabbit anti-cytokeratin 8 (Abcam ...
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bioRxiv - Biophysics 2021Quote: ... Cytokeratin 7 (K7, 1:200; Abcam #ab9021), and epithelial cell adhesion molecule (EpCAM ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: 7-Cl-KYNA was obtained from Abcam and dissolved in a small volume of 1M HCl ...
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bioRxiv - Developmental Biology 2023Quote: ... Rabbit monoclonal Cytokeratin 7 (Abcam Cat# ab68459) 1:200 ...
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bioRxiv - Developmental Biology 2023Quote: ... Rabbit monoclonal Cytokeratin 7 (Abcam Cat# ab181598) 1:200 ...
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CRISPR-SID: identifying EZH2 as a druggable target for desmoid tumors via in vivo dependency mappingbioRxiv - Cancer Biology 2021Quote: ... Slides were subsequently blocked with blocking buffer (3% natural serum, 1% BSA, 0.1% Tween-20) and incubated overnight with anti-EZH2 antibody (3748; Abcam), anti-CREB3L1 (AF4080 ...
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bioRxiv - Cell Biology 2022Quote: ... and anti-glyceraldehyde 3-phosphate dehydrogenase (GAPDH) primary antibodies (Catalog NO: ab181602) were purchased from Abcam (Cambridge, MA, USA). Other chemicals were of analytical grade and used without further purification.
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bioRxiv - Bioengineering 2022Quote: ... Primary antibody dilutions were prepared in 3% NGS in 1XPBS as follows: mouse anti-human vinculin (VIN-54, Abcam) at 1:200 and phalloidin conjugated with Alexa Fluor 546 (Invitrogen) ...
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bioRxiv - Neuroscience 2021Quote: ... plus 5% normal goat serum for 1 hr at RT before 3 d incubation in primary antibody diluted in block (1:1,000, chicken anti-GFP, Abcam, ab13970 ...
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bioRxiv - Neuroscience 2021Quote: Slices were placed into an open bath chamber and perfused constantly with aCSF (2-3 mL/min) supplemented with tetrodotoxin (0.5 μM; Abcam) and picrotoxin (50 μM ...
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bioRxiv - Neuroscience 2020Quote: ... Germany) and 0.5 ng/μl anti-Atxn3-(N-terminal)-tb (rabbit-anti-Ataxin-3, ab96316, Abcam, Cambridge, United Kingdom) were mixed in 1x detection buffer (in 50 mm NaH2 PO4 ...
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bioRxiv - Neuroscience 2020Quote: Cathepsin B activity was assayed in the cortex of 3-month old mice using a fluorometric kit (Abcam, #ab65300). Tissue was homogenised in lysis buffer then incubated for 30 minutes on ice ...
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bioRxiv - Neuroscience 2021Quote: Levels of NT3 protein in serum and muscles were assessed using a Human Neurotrophin-3 ELISA Kit (#ab100615 Abcam). In this study the viral vector encoded the human prepro-NT3 gene ...
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bioRxiv - Microbiology 2022Quote: The cytotoxic effect of Gal-9 on Calu-3 cells was determined using an MTT assay kit (abcam, ab211091) according to manufacturer’s guidelines ...
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bioRxiv - Immunology 2021Quote: ... and the obtained values represent the ratio between the immunodetected protein and the glyceraldehyde 3-phosphate dehydrogenase (GAPDH, Abcam) loading control ...
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bioRxiv - Microbiology 2022Quote: ... then incubated for 1 h at room temperature in 20 mL TBS-T with 3% milk powder and 1:5,000 diluted anti-GFP antiserum (Abcam) while shaking with 180 rpm ...
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bioRxiv - Neuroscience 2023Quote: ... the sectioned slices were incubated for one hour at room temperature in blocking solution (10% normal goat serum and 0.3% Titron X-100 in 1x TBS) and incubated overnight in polyclonal rabbit anti-HA (1:1,000, #ab9110, Abcam) at 4°C ...
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bioRxiv - Immunology 2023Quote: ... ANKRD22 (Cat# ab11638) and glyceraldehyde-3-phosphate dehydrogenase (GAPDH) (Cat# ab 9485) were obtained from Abcam (Waltham, MA, USA). Anti-Stabin-1 (Cat# AB AB6021 ...
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bioRxiv - Biophysics 2024Quote: ... Attached cells were then fixed by methanol/acetic acid solution (3:1) stained with Perl’s Prussian blue with nuclear fast red as counterstain (Iron stain kit, ab150674, abcam). Slides were dehydrated (incubation with 75 ...
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bioRxiv - Microbiology 2023Quote: ... Membranes were washed 3 x 5min with TBST and placed in HrP-linked rabbit anti-mouse secondary antibody (Abcam) (Ab diluted to 0.5μg/mL in TBST for 2h at RT) ...
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bioRxiv - Neuroscience 2022Quote: ... and incubated overnight in 3% normal donkey serum and 0.3% PBST containing primary antibodies for GFP (1:2000, chicken anti-GFP, ab13970, Abcam), and/or TH (1:2000 ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were blocked in 3% PBS-BSA with 0.05% Tween-20 for 15 minutes and then incubated in primary antibody (NuMa: Abcam ab109262 ...
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bioRxiv - Neuroscience 2024Quote: ... Sections were incubated overnight in primary antibodies then washed (3 x 0.1M PBS) and incubated in secondary antibodies conjugated to Alexa-594 (1:200, Abcam) or Alexa-488 (1:200 ...
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bioRxiv - Biochemistry 2022Quote: ... incubated at 95 °C for 5 min and analyzed by SDS-PAGE and subsequent InstantBlue Coomassie (Abcam) staining.
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bioRxiv - Molecular Biology 2022Quote: ... containing 0.1% (v/v) Tween-20 (TBST) for 2h and probed overnight with 1:5000 diluted primary antibody (Abcam, ab167453). Unbound primary antibody was removed by washing the membrane with TBST thrice ...
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bioRxiv - Molecular Biology 2021Quote: ... Specimens were fixed in 4% formaldehyde (FA) and antibody labelings were using rat anti-BrdU (Abcam, ab6326). Immunofluorescence staining was performed as described before51 ...
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bioRxiv - Molecular Biology 2021Quote: ... and the chromatin was directly treated with 2μL of anti-H3K9me2 antibody (Abcam 1220, Lot GR3308902-4). Barcode-indexed sequencing libraries were generated from reverse-crosslinked ChIP-DNA samples using a Kapa Hyper DNA Library Preparation Kit (Kapa Biosystems-Roche ...
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Single cell transcriptomics reveals the effect of PD-L1/TGF-β blockade on the tumor microenvironmentbioRxiv - Genomics 2020Quote: ... 4 μm sections were cut and placed on glass slides for CD3 antibody staining (clone SP7, Abcam). An IHC score was blindly assigned to each stained tumor section based on the observed density of CD3 cells across the entire slide (1 to 5 ...
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bioRxiv - Neuroscience 2023Quote: ... cells were treated with 4 μg/ml of Recombinant Mouse α-Syn Protein Aggregate (Active) (Abcam, ab246002) for periods of 24 ...
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bioRxiv - Cancer Biology 2024Quote: ... Primary antibodies were incubated overnight at 4℃ at the indicated dilutions: goat anti-YFP (1:400, Abcam) and rabbit anti-SPC (1:1000 ...
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bioRxiv - Molecular Biology 2024Quote: ... Cells were then stained with an antibody against enhancer of mRNA decapping 4 (EDC4) (1:200, Abcam) at room temperature for 1 h before washing three times with PBS ...
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bioRxiv - Neuroscience 2023Quote: ... Equal aliquots of sonicated chromatin were used per immunoprecipitation reaction with 4 μl H3K27ac antibody (Abcam; 4729) preconjugated to Protein G Dynabeads (Life Technologies) ...
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bioRxiv - Cancer Biology 2022Quote: ... 0.5 mM EGTA and protease inhibitor cocktail) and then incubated with 4 μg H3k27ac antibody (Abcam, ab4729) overnight at 4 C ...
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bioRxiv - Developmental Biology 2022Quote: ... The cells were incubated at 4 overnight with anti-O-GlcNAc antibody RL2 (1:400 ab2739, Abcam) or Alexa Fluor 660 labeled HaloTag-CpOGACD (10 μg/mL) ...
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bioRxiv - Neuroscience 2022Quote: ... and then incubated overnight at 4℃ with primary antibodies: rabbit anti-GFP (1:3000, Abcam, cat# ab6556), chicken anti-GFP (1:2000 ...
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bioRxiv - Immunology 2023Quote: ... Samples (4µg) were resolved on a premade 4–20% Mini- PROTEAN® TGX™ Precast Gel (Abcam).
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bioRxiv - Microbiology 2024Quote: ... (4%-12% Nupage Bis-Tris gel, Thermo Fisher Scientific, Waltham MA)(Antibody: ab207169, ab174293, 1:2000, Abcam, Cambridge MA ...
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bioRxiv - Genomics 2024Quote: ... 4-μm sections were stained with hematoxylin and eosin (H&E) using H&E Stain Kit (Abcam) per the manufacturer’s protocol or using the appropriate antibodies following antigen retrieval as previously described143 ...
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bioRxiv - Evolutionary Biology 2020Quote: ... then washed the 3 times in 1XPBS 5min at RT and incubated with the secondary antibody (1:200, Abcam ab175471) for 2-3h at RT ...
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bioRxiv - Molecular Biology 2021Quote: ... the 5’ adapter (containing four random nucleotides at the 3’ end) was ligated to the small RNAs using T4 RNA ligase (Abcam) for 1h ...
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bioRxiv - Cell Biology 2022Quote: ... 3 μL of antibody per IP (Cas9 – Diagenode C15310258; MRE11 – Novus NB100-142; γH2AX – Abcam ab81299; 53BP1 – Novus NB100-305) was added and placed on rotator for 1-2 h ...
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bioRxiv - Bioengineering 2020Quote: ... Cells were blocked with 1% BSA-0.3 M glycine in PBST and incubated with mouse anti-human integrin αvβ3 IgG (1:200, monoclonal, LM609, Abcam, ab190147) in 1% BSA-PBST overnight at 4°C ...
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bioRxiv - Bioengineering 2020Quote: ... Immunostaining was performed with specific primary antibodies (anti-TH, anti-GFAP, anti-NG2, anti-TMEM119, anti-pSer129-αSyn, anti-Cleaved Caspase-3, anti-CD68; Abcam), (anti-MAP2 ...
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bioRxiv - Biochemistry 2020Quote: ... 0.05% Triton-X 100) and subsequently incubated O/N with primary antibodies (3-pHis: rabbit, SC44-1 and F4/80: rat, ab6640; abcam) diluted in blocking buffer ...
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bioRxiv - Biochemistry 2020Quote: ... and M2 activated (n = 3) was reported as fold-change from control using a commercial intracellular ROS fluorescence assay kit (Abcam, used according to manufacturer’s instructions) ...
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bioRxiv - Cancer Biology 2021Quote: ... cells were fixed and permeabilized before incubation with the FITC-conjugated caspase-3 antibody following manufacturer’s instructions for subsequent flow cytometry analysis (Abcam, #65613). To assess membrane lipid perodixation ...
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bioRxiv - Cancer Biology 2020Quote: ... cells were treated with 10 μM TG100-115 for 3 days and conditioned medium was collected as described above followed by ELISA assay using human IL11 ELISA kit (Abcam).
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bioRxiv - Systems Biology 2021Quote: ... cells were incubated with 3% BSA and 0.1% Triton X-100 for 30 min at RT and stained with anti-gamma H2AX (Abcam 2893), anti CENP B (Santa Cruz ...
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bioRxiv - Biochemistry 2022Quote: ... Then membranes were washed (3×) with 1 × PBST for 10 min each wash and incubated with anti-Rabbit (diluted 1:5000, ab6721, Abcam) or anti-Mouse (1:3000 ...
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bioRxiv - Cell Biology 2020Quote: ... for 6 or 20 hours before inducing degradation of GRASP55 and/or GRASP65 by addition of 500 μM indole-3-acetic acid (IAA, Abcam). The Golgi ribbon was disassembled into single stacks by treatment with 3 μg/ml nocodazole (EMD Millipore ...