Labshake search
Citations for abcam :
2051 - 2100 of 3578 citations for 2H Pyrazolo 4 3 c pyridine 3 chloro 4 5 6 7 tetrahydro since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2022Quote: ... We used 3nM DRAQ-7 for live/dead stain (Abcam). Flow cytometric analysis was performed on a BD FACSAria II with special order 445nm laser for CFP detection when using Zebrabow (BD Biosciences) ...
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bioRxiv - Cancer Biology 2023Quote: ... 2’,7’-dichlorodihydrofluorescein diacetate (DCFDA/H2DCFDA) Cellular ROS Assay (abcam) and MitoSOX Red Mitochondrial Superoxide Indicator (invitrogen ...
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bioRxiv - Molecular Biology 2024Quote: ... anti-RDE-1(7) and anti-ACTIN (catalog #AB49900, Abcam). Secondary antibodies directed against the primary antibody Sheep anti-mouse IgG HRP (Catalog #515-035-062 ...
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bioRxiv - Molecular Biology 2021Quote: ... The membrane was blocked in 5% milk for 1h followed by incubation with primary antibodies: c-Myc (Abcam ab185656; 1:1000), Fos (Cell Signaling 2250S ...
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bioRxiv - Neuroscience 2022Quote: ... in a heating chamber (37°C) in which the choroid plexus was loaded with Fluo-8 AM (5 μM, 30 min, Abcam, ab142773) with subsequent continuous wash with HEPES-aCSF (~1 ml/min ...
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bioRxiv - Microbiology 2024Quote: ... The cells were then treated with 5% BSA in PBS for 90 minutes at 25°C followed by treatment with anti-Listeria antibody (Abcam: ab35132) at 25°C for 2 hours ...
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bioRxiv - Molecular Biology 2023Quote: ... mouse monoclonal Abs (mMAbs) against lamin A/C C-terminus residues aa 319-566 (LA/C-C, [131C3], ab18984, Abcam, Cambridge, UK) and lamin A/C N-terminus residues aa 2-29 (LA/C-N ...
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bioRxiv - Genomics 2020Quote: ... and immunoprecipitation was performed using 4 μg of the appropriate antibody (anti-CTCF: 61311, Active Motif; anti-RAD21: ab88572, Abcam; anti-GFP: ab290, Abcam). Paired-end sequencing was performed on a NovaSeq6000 platform (Illumina ...
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bioRxiv - Neuroscience 2020Quote: ... and incubated overnight in 4% NGS containing an antibody targeting αsyn phosphorylated at serine 129 (anti-pSer129, rabbit monoclonal, 1:600, Abcam #51253). Subsequently ...
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bioRxiv - Neuroscience 2020Quote: ... Samples were resolved by SDS-PAGE using a 4-15% gradient gel and analyzed by Western blot using the following primary antibodies: rabbit anti-GFP (Abcam Ab290), rabbit anti-Cx34.1 3A4-conjugated-680LT ...
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bioRxiv - Cell Biology 2021Quote: ... cut based on the molecular weights indicated by the prestained marker and then incubated overnight on a shaker at 4 oC with rabbit anti-human STEAP3 (1:1000) (abcam, 151566), mouse anti-human p53 (1:1000 ...
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bioRxiv - Immunology 2022Quote: ... and CTLA-4 Ig (Abatacept) were generated using the APC Conjugation Kit - Lightning-Link® according to manufacturer’s instructions (Abcam, ab201807). APC conjugated anti-PD-L1 antibodies were procured from Biolegend (clones MIH1 ...
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bioRxiv - Microbiology 2020Quote: ... endogenous human NPC1 and HSP90 were purified using immobilized Recombinant Protein G Resin (Generon) and 4 µg of specific antibodies against NPC1 (Abcam, ab108921) or HSP90 (Enzo Life Sciences ...
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bioRxiv - Neuroscience 2022Quote: ... Samples were resolved by SDS-PAGE using a 4–15% gradient gel and analyzed by Western blot using the following primary antibodies: chick anti-GFP (Abcam Ab13970), rabbit anti-Cx34.1 3A4-conjugated-680LT ...
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bioRxiv - Physiology 2020Quote: ... or guinea pig anti-glucagon (PCA2/4, 1:200; raised in-house) with rabbit anti-Ki-67 (1:200; Abcam ab15580) or TUNEL reaction mixture (Roche Diagnostics Ltd ...
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bioRxiv - Biophysics 2020Quote: ... A negative control was performed on a subset of the wells by treating cells with a spike of 10 µM Carbonyl cyanide 4-(trifluoromethoxy)phenylhydrazone (FCCP, Abcam ab120081) 10 minutes prior to the application of TMRE ...
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bioRxiv - Physiology 2023Quote: ... Frozen sections were fixed with fresh 4% paraformaldehyde and neutrophil elastase protein was detected using anti-neutrophil elastase antibody (Abcam #68672).
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... The membrane was blocked for nonspecific binding with 5% milk in 1x TBST (Tris-buffered saline, 0.1% Tween 20) and incubated with rabbit polyclonal anti-4-HNE (ab46545, Abcam, 1:1500) and HIF-1α (141795 ...
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bioRxiv - Biochemistry 2023Quote: ... Fractions were analyzed by separation using sodium dodecyl sulfate polyacrylamide gel electrophoresis (4–12% polyacrylamide) and staining with InstantBlue Coomassie Protein Stain (Abcam, UK). Fractions containing chalcone synthase were combined and loaded onto a HiLoad 16/600 Superdex 200 pg column ...
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bioRxiv - Cancer Biology 2024Quote: ... 20 μL of GBO medium containing 1 mM ACh with or without a combination of the M3 muscarinic receptor antagonist 4-DAMP (100 μM) and pan-nicotinic receptor antagonist mecamylamine (100 μM, Abcam, ab120459) was added to each GBO at the ALI surface 30 minutes prior to imaging with the same conditions ...
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bioRxiv - Genetics 2022Quote: ... 500 ug lysate was incubated at 4□ overnight with 2 ug of appropriate antibodies (anti-H3K4me3 (Abcam, Cat# ab8580, RRID: AB_306649), anti-H3K36me3 (Abcam ...
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bioRxiv - Cell Biology 2022Quote: Grown BEC-organoids were treated with the FA-mix for 4 days and triglyceride levels were measured with a Triglyceride kit (Abcam, ab65336) following the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2022Quote: ... After three washes with 1 x PBS the samples were incubated for 4 hours with anti-mouse secondary antibodies conjugated with Alexa Fluor 488 (ab150077, Abcam, UK) diluted 1:400 in PBS ...
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bioRxiv - Neuroscience 2022Quote: ... Anti-phospho Syn S129 (1:500, ab-59264), and anti-4-HNE (1:2000, ab-46545) were from Abcam (Cambridge, UK). Anti-α-Syn (1:1000 ...
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bioRxiv - Biochemistry 2023Quote: ... Pellets were washed and resuspended in PBS and subsequently incubated with 4 μg/ml of PE-conjugated GABARAP/GABARAPL1/GABARAPL2 antibody (Abcam, ab223948) for 1 h ...
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bioRxiv - Biochemistry 2023Quote: ... Denatured samples were resolved in 4-12% Bis-Tris SDS-PAGE gradient gels and stained with InstantBlue® Coomassie protein stain (Cat#ab119211, abcam). Protein bands were excised from gel ...
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bioRxiv - Neuroscience 2024Quote: ... Equal aliquots of sonicated chromatin were used per immunoprecipitation reaction with 4 ul H3K27ac antibody (Abcam, cat #4729; lot # GR323132-1) preconjugated to Protein G Dynabeads (Life Technologies) ...
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bioRxiv - Genomics 2024Quote: ... blocked with blocking solution (PBS + BSA 4% + 0.025% Tween 20) and incubated with an anti-p65 antibody (1:200 Abcam Cat #ab16502) overnight at 4°C ...
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bioRxiv - Bioengineering 2024Quote: ... to deionized water and fixed in 4% PFA for 15 min before staining with picrosirius red according to the manufacturer’s instructions (Abcam, cat#: ab150681). Immunostaining ...
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bioRxiv - Biochemistry 2024Quote: ... Cells were transduced with the Sh2 or Scr control constructs on 4 DIV and cellular ATP measurements were performed on 18 DIV using a Luminescent ATP Detection Assay Kit (Abcam, ab113849). To block luminescence signal contamination from adjacent wells ...
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bioRxiv - Biochemistry 2024Quote: ... Eluted proteins were resolved on NuPAGE™ 4–12% Bis–Tris gel followed by Western blotting using anti-IFNAR1 antibody (ab124764, Abcam).
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bioRxiv - Cancer Biology 2024Quote: ... 4 µm-thick TMA slides were incubated with the primary CD14 monoclonal antibody (clone 1H5D8, Abcam, Cat# ab181470, Camdridge, MA, USA) at a dilution of 1:3,000 for 15 minutes at room temperature ...
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bioRxiv - Cell Biology 2024Quote: ... The 2 or 4 µm section was used for H&E staining and IHC of anti-human CD11b mAb (abcam #ab307523). The samples were observed by Nikon Eclipse Ti2 microscope carrying NIS-Elements imaging software.
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bioRxiv - Developmental Biology 2021Quote: Apoptosis and proliferation levels were measured by immunostaining in dewaxed and rehydrated midline placental sections with cleaved caspase-3 (Asp175) (Cell Signalling, US, 9661; 1:200) and Ki67 (Abcam, UK, ab264429; 1:500). Sections were incubated with goat-anti-rabbit secondary antibody (Abcam ...
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bioRxiv - Immunology 2021Quote: ... Slides were then washed 3×10 minutes in PBS at RT and again blocked with 10% normal donkey serum (Abcam, ab7475, Lot GR3234297-32) for 20 minutes at room temperature before being incubated with PBS containing both WGA-555 (Thermo Fisher ...
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bioRxiv - Immunology 2021Quote: ... Slides were then washed 3×10 minutes in PBS at RT and again blocked with 10% normal donkey serum (Abcam, ab7475, Lot GR3234297-32) for 20 minutes at room temperature before being incubated with PBS containing WGA-555 (Thermo Fisher ...
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bioRxiv - Neuroscience 2020Quote: ... Sections were washed 3 times with 1x PBS and placed in secondary (1:500 AF488 conjugated Goat anti-Rabbit (Abcam, Cambridge, UK, Cat# AB150077), 1:500 Cy3 conjugated Donkey anti-mouse (Jackson Immuno Research Labs ...
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bioRxiv - Cancer Biology 2023Quote: ... The lower part of the membrane was blocked with 3% BSA in phosphate-buffered saline (PBS) solution followed by incubation with rabbit polyclonal anti-GFP (Abcam, UK; #ab290, 1:1,000) primary antibody at RT for 1 h for eGFP detection (30 kDa) ...
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bioRxiv - Neuroscience 2024Quote: Western blot analysis was performed on hCMEC/D3 cell lysates using antibodies against full-length and cleaved caspase-3 (Abcam, ab32351, 1:500 dilution), VE-Cadherin (Cell Signalling ...
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bioRxiv - Neuroscience 2024Quote: ... except that brains were incubated overnight in apoptosis-related primary antibodies (H2A.X, 1:500, EMD Millipore, 05-636, made in mouse; and caspase 3, 1:500, abCam, AB13847, made in rabbit) overnight in blocking buffer at 4°C with gentle rotation ...
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bioRxiv - Plant Biology 2024Quote: ... Membranes were washed in TBS-T for 3 × 10 minutes incubating with secondary anti-rabbit (Cell Signalling Technology catalogue # 7074S; 1:2,000) and anti-mouse (Abcam Ltd. Catalogue # ab6728; 1:5,000) in TBS-T with 5% milk for one hour at RT ...
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bioRxiv - Cell Biology 2020Quote: ... Col 6 (Abcam ab6588, 1:500), Hoescht 33342 (ThermoFisher 62249 ...
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bioRxiv - Physiology 2022Quote: ... IL-6 (ab119548; Abcam, United Kingdom), TNF-alfa (ab46070 ...
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bioRxiv - Genomics 2020Quote: ... 6 μg anti-H3K36me2 (Abcam, ab9049), 6 μg anti-H3K4me3 (Abcam ...
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bioRxiv - Genomics 2020Quote: ... 6 μg anti-H3K4me3 (Abcam, ab8580), 10 μg anti-Ser5-P (Active Motif ...
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bioRxiv - Genomics 2020Quote: ... 6 μg anti-H3K36me3 (Abcam, ab9050), 6 μg anti-H3K36me2 (Abcam ...
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bioRxiv - Genomics 2020Quote: ... OCT4 (Abcam, ab109183, lot gr120970-6), and H3K4me2 (Upstate 07–030 ...
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bioRxiv - Cell Biology 2021Quote: ... Anti-CXCL5/6 antibody (ab198505; Abcam), Mouse anti-β-actin (sc-47778 ...
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bioRxiv - Developmental Biology 2021Quote: ... mouse anti-cytokeratin 6 (Abcam, ab18586), donkey anti-rabbit–Cy3 (Jackson Immuno Research ...
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bioRxiv - Physiology 2024Quote: ... interleukin-6 (ELISA Kit, ab222503, Abcam) and TNFα (ELISA Kit ...