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Citations for abcam :
1701 - 1750 of 2348 citations for Porcine Circovirus 2 PCV2 Protein since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2024Quote: ... containing a 1 base pair insertion and a selection cassette in exon 2 were obtained from Abcam and were cultured in DMEM (Gibco ...
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bioRxiv - Neuroscience 2024Quote: ... followed by a 2-hr incubation in secondary antibodies: goat anti-chicken AF555 (AB150170, Abcam, 1:2000) and donkey anti-rabbit AF488 (A21206 ...
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bioRxiv - Neuroscience 2024Quote: ... 150 µg of synaptosome extracts were mixed with 2 µg of either rabbit anti-GluN2B (Abcam, ab65783) or rabbit IgG isotype control antibody (Thermo Fisher 10500C ...
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bioRxiv - Molecular Biology 2024Quote: ... Antibodies were sourced for GABARAPL1 (#1: Proteintech, #11010-1-AP; #2 Abcam, #ab86497; #3: Cell Signaling, #26632), LC3B (Cell Signaling #2775) ...
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Decreased Astrocytic CCL5 by MiR-324-5p Ameliorates Ischemic Stroke Injury via CCR5/ERK/CREB PathwaybioRxiv - Neuroscience 2024Quote: ... The PVDF membranes were blocked with 5% BSA and probed with primary antibodies (phospho-ERK1/2, Abcam, ab76299 ...
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bioRxiv - Immunology 2024Quote: ... incubated at RT for 2 hours with HRP Anti-6X His tag® antibody (1:1000) (Abcam, Cambridge ...
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bioRxiv - Cancer Biology 2024Quote: Cells/Organoids were treated with the following compounds as indicated: 5FU ( Abcam, ab142387-5g, 2-5 µM), Metformin (Cayman Chemicals ...
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bioRxiv - Cancer Biology 2024Quote: ... Cells were then selected with puromycin (Abcam; 2 μg/ml for Huh7, 7 μg/ml for HepG2) until the control cell population exhibited complete lethality ...
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bioRxiv - Cell Biology 2024Quote: ... 2) Rabbit polyclonal IgG anti-lamin B1 (ab16048; 1 mg/mL; dilution 1:800; Abcam, Cambridge, UK) for immunostaining of lamin B1 ...
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bioRxiv - Biophysics 2020Quote: ... All sections were permeabilized with 0.2% Triton X-100 in PBS for 15 minutes and non-specific protein binding was then blocked by incubation in a commercial blocking agent (ab156024, Abcam) for 1 hour at room temperature ...
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bioRxiv - Cancer Biology 2021Quote: Antibodies against phospho-Heat Shock Protein 27 (S82) and phospho-c-Jun (S73) were obtained from Abcam (Cambridge, United Kingdom), while phospho-JNK (T183/Y185 ...
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bioRxiv - Cell Biology 2020Quote: ... Eluted proteins were analyzed on a 6% SDS-PAGE gel followed by a western blot using monoclonal anti-GST (Abcam) or anti-MBP (New England Biolabs ...
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bioRxiv - Genomics 2021Quote: ... one piece corresponding to the higher protein ladder size for the incubation of RAD21 and CTCF primary antibodies (RAD21: anti-RAD21 antibody, rabbit polyclonal, Abcam, ab154769 ...
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bioRxiv - Microbiology 2022Quote: ... α-surfactant protein D (primary: polyclonal, Abcam catalog no. ab203309; secondary: Donkey anti-Rabbit IgG, AlexaFluor 405, Abcam catalog no. ab175651), α-mannose binding lectin A (primary ...
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bioRxiv - Molecular Biology 2021Quote: Expression of GLIS1-βGAL fusion protein was examined by staining tissue sections with chicken anti-βGAL (1:500, ab9361, Abcam) and Alexa Fluor@ 488 donkey anti-chicken IgG (1:2000 ...
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bioRxiv - Molecular Biology 2021Quote: ... Immunoprecipitations for H2A.Z and HA-tag were performed similarly on benzonase-treated yeast cell extract using 36µl Protein-A sepharose beads coupled with 6µl of appropriate antibody (Anti-H2A.Z, Active Motif 39647; Anti-HA tag, Abcam ab9110). Control immunoprecipitations (mock ...
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bioRxiv - Microbiology 2020Quote: ... Membranes containing proteins were blocked in 5% BSA and incubated with rabbit anti-HMGB1 polyclonal antibody (1:1,000, Abcam, U.S.A), followed by incubation with horseradish peroxidase-linked secondary antibodies (1:5,000 ...
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bioRxiv - Microbiology 2020Quote: ... Primary antibodies were applied over night at a dilution of 1:100 for SARS-CoV S protein (monoclonal mouse, ab272420, Abcam; microwave ...
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bioRxiv - Bioengineering 2021Quote: ... and stained for SARS-CoV2 N protein using Alexa488-labelled-CR3009 antibody produced custom (see section for Recombinant mAb production) and cellular DNA using DRAQ7 (ABCAM). Whole-well imaging at 5x was carried out using an Opera Phenix (Perkin Elmer ...
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bioRxiv - Cancer Biology 2021Quote: ... for 6 h and then fixed with methanol for immunostaining with an antibody against nuclear pore complex proteins (clone mAb414, Abcam) following the regular immunofluorescence protocol described below ...
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bioRxiv - Biochemistry 2021Quote: ... The immunoprecipitation of HA-tag proteins was performed overnight in a total volume of 600 μl with 4 μg of HA-antibody (ab9110, Abcam) except the 5% of samples taken as input ...
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bioRxiv - Cell Biology 2021Quote: ... ovalbumin and CD4-Fc fusion proteins were labelled with allophycocyanin (APC) with the Lightning-Link Labelling Kit (Abcam, Cambridge, UK).
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bioRxiv - Cell Biology 2021Quote: ... Non-specific binding was blocked by incubating the membranes with 5% nonfat milk for 2 h at room temperature and the identity of proteins evaluated by incubation at 4 ℃ overnight with the following primary antibodies (Abcam): HSP70 (1:1000 ...
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bioRxiv - Cell Biology 2021Quote: ... 500 µg of total protein extract were used to IP with either 2.5 µg V5 rabbit polyclonal antibody or VPS35 mouse monoclonal over night at 4°C and recovered after incubating for 6h at 4°C in a rotator with equilibrated Agarose Protein-G beads (Abcam). Immunoprecipitation followed by MS analysis was performed as described but using magnetic Protein G beads (Thermo Scientific) ...
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bioRxiv - Neuroscience 2020Quote: ... mouse anti-mouse postsynaptic density protein 95 (PSD-95, 1:1000 for cells, 1:200 for tissues, Abcam, catalog ab99009), rabbit anti-mouse vesicular gamma aminobutyric acid (GABA ...
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bioRxiv - Cell Biology 2021Quote: Iron saturation of transferrin was measured on 6% TBE-urea gels as previously described.32 Gels were stained with InstantBlue Coomassie protein stain (abcam).
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bioRxiv - Cell Biology 2021Quote: ... The overexpression of WT and mutant c-Myc protein in cells were confirmed by immunofluorescence staining of transfected cells with anti-c-Myc antibody (Abcam). The sequences of cloning primers were listed in Table S4.
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bioRxiv - Immunology 2021Quote: ... the HIV Env 1086.c K160N gp120 protein was conjugated to biotin using the Fast Type A Biotin Conjugation kit (Abcam) and then captured on avidin-labeled fluorescent beads (NeutrAvidin™ ...
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bioRxiv - Physiology 2022Quote: 10 µg of protein from 4-week-old adrenals were used for measurement with PKA kinase activity kit (ab139435, Abcam).
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bioRxiv - Biochemistry 2022Quote: ... The supernatants were incubated with an anti-TIN1 antibody followed by protein-A/G-agarose beads (Abmart) or with anti-GFP Sepharose beads (Abcam) at 4 °C for 6 h ...
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bioRxiv - Physiology 2022Quote: ... blocked in 5% goat serum for 1 hour and stained using myelin protein zero (MPZ) (1 in 50 in 5% goat serum in PBS containing 1% triton) (EPR20383, Abcam) overnight ...
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bioRxiv - Biochemistry 2022Quote: ... The molecular masses of the protein bands were assessed by comparing with the mass of the bands in the lane containing the standards (Abcam). The experiment was performed in triplicate and the bands corresponding to the heterodimer and heterotetramer were visualized in all the three experiments.
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bioRxiv - Biochemistry 2022Quote: ... Separated proteins were transferred to polyvinylidene difluoride (PVDF) membranes and immunoblotted with antibodies specific for SF3B1 (Abcam, catalog no. ab170854), SF1 (Bethyl Laboratories ...
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bioRxiv - Biophysics 2022Quote: ... plus antibodies against respective caveolin accessory proteins as indicated in figures (rabbit anti-cavin1, 1:200, Abcam 76919; goat anti-EHD2, 1:200, Abcam 23935 ...
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bioRxiv - Biophysics 2022Quote: ... 1:1000 dilution in blocking buffer) plus antibodies against respective caveolin accessory proteins as indicated in figures (rabbit anti-cavin1, 1:200, Abcam 76919 ...
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bioRxiv - Cell Biology 2022Quote: ... The following commercial polyclonal and monoclonal antisera were used (m, mouse; h, human and r, rat proteins): anti-LIMPII (ab165222) was from Abcam; anti-Calnexin (2679) ...
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bioRxiv - Developmental Biology 2022Quote: ... 100µg of cleared chromatin (protein) was incubated with 2µg of the following antibodies overnight at 4°C: H3K27ac (Abcam, ab4729), H3K4me1 (Abcam ...
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bioRxiv - Immunology 2020Quote: The iNOS recombinant protein (full length) was procured from sigma and Nitric Oxide Synthase activity assay kit was from Abcam. The NOSIP (full length ...
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bioRxiv - Immunology 2021Quote: ... AD-01 (NH2-QIRQQPRDPPTETLELEVSPDPAS-OH) (Almac Group, United Kingdom), ALM201 (NH2-IRQQPRDPPTETLELEVSPDPAS-OH) (Almac group, United Kingdom) and recombinant FKBPL protein (ab98117; Abcam). All experiments were carried out at 37°C in a humidified atmosphere of 95% O2/5% CO2.
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bioRxiv - Microbiology 2020Quote: ... Co-IP for NPC1 was performed using 50μl of the Immobilized Recombinant Protein G Resin (Generon) and specific antibodies against NPC1 (Abcam, ab108921). The cell pellets were incubated for 30 minutes on ice with 200μl lysis buffer ...
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bioRxiv - Microbiology 2020Quote: ... Samples were then mixed with either anti-HA magnetic beads (Thermoscientific) or protein G magnetic beads (Thermoscientific) coupled with anti-pADPr (Abcam) (beads were pre-blocked with 1% BSA) ...
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bioRxiv - Molecular Biology 2021Quote: ... the cells were permeabilized with 0.1% Triton X 100 in 1X PBS for 5 min and blocked with Protein Block (Abcam, USA) for 20 min at room temperature ...
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bioRxiv - Neuroscience 2022Quote: PPGYFP NTS tissue was processed free-floating and incubated with chicken anti-green fluorescent protein (GFP; AbCam, ab13970, 1:1000) and/or rabbit anti-c-FOS (Calbiochem ...
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bioRxiv - Immunology 2022Quote: ... were washed with lysis buffer (containing protein inhibitors) and incubated with 5 µg of 4C4 antibody or IgG1k as a control isotype (ab18443 Abcam) at 4°C overnight under rotation ...
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bioRxiv - Immunology 2022Quote: ... permeabilized with 0.1% Triton X-100 in PBS and stained overnight at 4°C with ACE2 protein-specific antibody (Abcam Ab15348). Cells were then incubated with anti-rabbit secondary antibody (Alexa Fluor 536 anti-rabbit ...
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bioRxiv - Neuroscience 2020Quote: ... 2019) using antibodies against cAMP response element-binding protein (CREB; clone LB9, produced in mouse, #ab178322, AbCam, Cambridge, UK; RRID:AB_2827810), phospho-CREB (Ser133 ...
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bioRxiv - Physiology 2021Quote: ... Blots were incubated overnight in rabbit anti-mouse primary antibody for proteins of interest (Supplemental Table S4) and with horseradish peroxidase-conjugated goat anti-rabbit IgG secondary antibodies (ab6721, Abcam). Proteins of interest were detected by incubating blots in ClarityTM Western ECL Blotting Substrate (1705061 ...
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bioRxiv - Neuroscience 2020Quote: ... Dilutions of the antibodies were as follows: chicken anti glial-fibrillary acidic protein (GFAP2; 1:1,1000; Abcam, Cambridge, United Kingdom); mouse anti-microtubule associated protein (MAP2 ...
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bioRxiv - Neuroscience 2020Quote: ... Sections used for non-vascular associated protein visualization were incubated overnight in primary antibodies: rabbit anti-MBP (1:200, Abcam), or rat anti-MBP (1:200 ...
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bioRxiv - Bioengineering 2021Quote: ... the corresponding protein bands were probed with a goat anti-rabbit horseradish peroxidase-conjugated antibody (1:5000 dilution, Abcam, ab205718) for 30 min ...