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Citations for abcam :
1451 - 1500 of 10000+ citations for Flap endonuclease 1 FEN 1 Human since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2021Quote: ... sc84663 [santa-cruz], 1/1000 dilution), anti-Pol2tot (rabbit, A300-653A [Béthyl] 1/400 dilution, anti-Pol2Ser2P (rabbit, ab5095 [abcam] 1/100 dilution) and anti-Pol2Ser5P (rabbit ...
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bioRxiv - Neuroscience 2021Quote: ... were subjected to western blotting using the indicated primary (mouse anti-GABAA receptor alpha 1 #ab3299, Abcam, 1:200; rabbit antibeta actin #ab8227, Abcam, 1:1000) and secondary antibodies ...
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bioRxiv - Developmental Biology 2022Quote: Primary antibodies used included those against α-tubulin (T5168, Sigma, ascites fluid, 1 in 5000 and ab4074, Abcam, 1 μg ml-1) polyglutamylated tubulin B3 (T9822 ...
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bioRxiv - Genetics 2022Quote: ... 1:1000; Anti-GAPDH mouse, Abcam ab8245, 1:10,000; Anti-nitrotyrosine, Merck 05-233, 1:100; Anti-GAPDH rabbit, Abcam ab9485, 1:1000), 3x 5-min washes with PBS-T ...
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bioRxiv - Cell Biology 2024Quote: The primary antibodies are as follows: anti-NOX2/gp91phox (ab129068, dilution 1:400), anti-ACTB (ab8227, 1:2000), anti-OCN (ab93876, 1:500) from Abcam (Cambridge, UK); anti-SOD2 (ADI-SOD-111 ...
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bioRxiv - Immunology 2024Quote: ... anti-beta Actin HRP [AC-15] (#ab49900, diluted at 1:10000), anti-IL-1 beta (#ab2105, diluted at 1:1000) antibodies from Abcam (Cambridge, UK), anti-AMPKα2 (#AF2850 ...
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bioRxiv - Cancer Biology 2023Quote: ... was performed for the HLA class I heavy chain (HCA2, 1:1000, Nordic Bio, MUB2036P; HC10, 1:3200, Nordic Bio, MUB2037P) and β-2m (EPR21752-214, 1:4000, Abcam, ab218230) on all generated TMAs as described previously42 ...
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bioRxiv - Synthetic Biology 2023Quote: ... The membrane was washed with TBS-T and incubated for 1 hour at room temperature with a 1:10,000 dilution of HRP-conjugated goat anti-mouse secondary antibodies (Abcam ab6789-1 MG) in 5% milk-TBS-T ...
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bioRxiv - Molecular Biology 2022Quote: ... Ectopic huLiver tissue was processed and stained using the methods described above with polyclonal rabbit anti-MSP1 (1:100) and monoclonal mouse anti-EBA-175 (1:100) followed with goat anti-rabbit IgG (1:100) (Abcam, Cambridge, UK), and goat α-mouse IgG1 (1:100 ...
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bioRxiv - Genomics 2023Quote: ... cells were blocked for 1 h in 1×PBS + 2% w/v BSA and then incubated with mouse anti-E-cadherin (1:1000) (Abcam, cat#231303) and chicken anti-vimentin (1:200 ...
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bioRxiv - Cancer Biology 2023Quote: ... Antibodies used were (H3K27me3 Cell Signaling C26B11 1:500, EZH2 Cell Signaling 5246S 1:200, B2M Millipore MABF1968 1:2000, HLA-DR,DQ,DP AbCAM ab7856 1:500 ...
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bioRxiv - Bioengineering 2023Quote: ... Cryosections were blocked with 1% bovine serum albumin in PBS for 1 h at room temperature and incubated with primary antibodies (anti-perilipin 1, PA5-72921, 1:200, ThermoFisher, Waltham, MA, USA; anti-laminin, ab11575, 1:200, Abcam, Cambridge, UK) diluted in 1% BSA in PBS ...
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bioRxiv - Cancer Biology 2023Quote: ... and 0.05% Tween 20 in PBS for 1 hr followed by incubation in primary antibodies ((MYC 1:100, Abcam Cat# 32072, γH2AX 1:100 Abcam Cat# 303656) for overnight at 4°C in a humid chamber ...
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bioRxiv - Cancer Biology 2024Quote: ... blocked in 5% normal serum in 1× PBS Triton 0.3% for 1 hr at room temperature and then incubated with the primary antibody (anti-S100b diluted 1:1000, Abcam, ab5264 or anti-RPE65 diluted 1:250, Thermo Fisher Scientific, MA1-16578; anti-CD34 diluted 1:200, abcam, ab8158) diluted in blocking solution for 1.5 hours at room temperature ...
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bioRxiv - Cell Biology 2024Quote: ... The levels of human PEDF and VEGF were determined using Human VEGF ELISA Kit (Abcam) and Human PEDF ELISA Kit (Abcam) ...
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bioRxiv - Biochemistry 2023Quote: ... Human amylase was detected in CVLs using an ELISA for human pancreatic amylase (Abcam ab137969) according to manufacturer’s instructions ...
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bioRxiv - Biophysics 2021Quote: ... the samples were incubated with the primary antibody (monoclonal mouse anti-vinculin, 1:100, Sigma V9264, St. Louis, USA; polyclonal rabbit anti-talin 1, 1:100, Abcam ab71333, Cambridge, UK) diluted in blocking solution for 1.5 h at room temperature or overnight at 4 °C.
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bioRxiv - Cell Biology 2021Quote: ... CD63 staining was performed by incubating with mouse anti-CD63 (TS63 Diaclone 857.770.000 1/200) in PBS-BSA 1% for 30 min and CD9 staining was performed incubating with rabbit anti-CD9 (Abcam ab236630 1/80) for 30 min ...
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bioRxiv - Cell Biology 2021Quote: ... CSN2 and beta-actin proteins using anti-BMAL1 (ab3350, dilution 1:1,000), anti-CSN2 (LS-C373659, Life Spam, dilution 1:200) and Anti-beta-actin (AbCam ab8227, dilution 1:10,000) antibodies ...
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bioRxiv - Cancer Biology 2022Quote: ... 1:400 dilution), rb anti-CD68 (#76437, MultiMab, CST, 1:200 dilution) and rb anti-CD16 (ab24622, clone EPR14336, Abcam, 1:400 dilution) antibodies were used for staining ...
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bioRxiv - Neuroscience 2022Quote: ... every sixth section throughout the entire striatum was stained with a cocktail of primary antibodies: TH (Pel Freeze, P40101, 1:1000), Iba1 (Wako, 019-19741, 1:1000) and GFAP (abcam, ab4674, 1:2000). As fluorescently labelled secondary antibodies ...
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bioRxiv - Neuroscience 2022Quote: ... rabbit anti-CaVβ3 (Alomone Labs #AAC-008, 1:800) or mouse anti-CaVβ4 (Neuromab #75-054, 1:1000) and rabbit anti-β-tubulin (Abcam #ab6046, 1:600,000). Membranes were then incubated in LiCor Blocking Buffer as described above and LiCor IRDye secondary antibodies ...
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bioRxiv - Neuroscience 2022Quote: ... To confirm that the Iba-1 antibody labeled resident microglia and not infiltrated macrophages we co-labeled the Iba-1 positive cells using goat anti Iba-1 antibody (Abcam ab5076, 1:125) with rabbit polyclonal antibody for rat TMEM119 that recognizes microglia-specific transmembrane proteins (Synaptic Systems GmbH ...
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bioRxiv - Neuroscience 2022Quote: ... FoxP2 (mouse, Merck Millipore: MABE415, RRID: AB_2721039; 1:2000; Invitrogen: MA5-31419, RRID: AB_2787055; 1:2000; rabbit, Abcam: ab16046, RRID: AB_2107107; 1:500), Calb1 (rabbit ...
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bioRxiv - Neuroscience 2022Quote: ... donkey anti chicken (Jackson 703-166-155, 1:1000), goat anti rabbit (Jackson 111-096-114, 1:1000) and donkey anti rabbit (Abcam AF555, 1:1000). Moreover ...
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bioRxiv - Microbiology 2020Quote: Paraffin embedded tissue sections were stained for SARS-CoV-2 nucleocapsid protein (1:500) and ionized calcium binding adaptor molecule 1 (Abcam; ab5076; 1:50) using a Leica Bond RXm automated staining instrument following permeabilization using 0.01% Triton X diluted in Trisbuffered saline (TBS) ...
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bioRxiv - Developmental Biology 2022Quote: ... the sections were blocked with 1 % BSA in PBS for 1 h at room temperature and incubated with rabbit anti-PDGFR-β (1:200, Abcam, Cambridge, England) overnight ...
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bioRxiv - Neuroscience 2022Quote: ... followed by overnight incubation with primary antibodies (CDKL5- 1:1000, #HPA002847, Atlas Antibodies-Sigma Aldrich; NMDAR1-1:1000, #700685, Thermo Fisher; NMDAR2A-1: 1000, #ab169873, Abcam; NMDAR2B- 1:1000 ...
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bioRxiv - Neuroscience 2022Quote: ... The brain sections of the rats with Gi-DREADDs were incubated first in a blocking solution (10% fetal horse serum and 0.15% Triton X-100 in PBS) for 1 h at 24 ± 1°C and then with primary rabbit anti-mCherry antibody (1:1000, Abcam, ab167453, Cambridge, UK) overnight at room temperature ...
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bioRxiv - Physiology 2022Quote: ... Rat anti-histone H3K9ac (active motif, #61663, 1:500), Rabbit anti-LaminB1(CST, #13435S, 1:2000) Rabbit anti-Beta-Tubulin (abcam, ab179513, 1:500), rabbit anti-pS6K(T398 ...
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bioRxiv - Biophysics 2023Quote: ... Blots were probed with antibodies for XRCC4 (CBMAB-X0197-YC, 1:2000), XLF (Antibodies ABIN2857060, 1:2000) and MLH1 (Abcam ab92312, 1:1000). Agilent NovoExpress Version 1.5.0 for the Quanteon was used to capture and analyze the flow cytometry data ...
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bioRxiv - Cancer Biology 2023Quote: ... Antibodies used for immunohistochemistry were anti-GFAP (Cell Signalling Technology; #3670, 1:1000), anti-STAT3 pY705 (Cell Signalling Technology; #3760, 1:400) and anti-GFP (abcam; #ab290, 1:400). Antibodies used for reverse phase protein array (RPPA ...
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bioRxiv - Immunology 2023Quote: ... slides were probed with primary antibodies (CD31 1:200, Biolegend, MEC13.3; mSPARCL1 1:500, R&D systems, #AF2836-SP; hSPARCL1 1:500, R&D systems, #AF2728-SP; ERG 1:2000, Abcam, #ab92513 ...
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bioRxiv - Cell Biology 2022Quote: ... including monoclonal anti-FAM76B (monoclonal, 1:1000, homemade (Zheng et al. 2016)) or IBA-1 (goat polyclonal, 1:1000, Abcam, Boston, MA, USA) antibodies ...
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bioRxiv - Cancer Biology 2024Quote: ... followed by blocking in 1% BSA for 1 hour at room temperature and then incubation with rabbit polyclonal anti-Fibronectin (Abcam ab2413, 1:100) diluted in antibody diluent (Life technologies ...
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bioRxiv - Cell Biology 2020Quote: ... and anti-α-Tubulin (Abcam, Cat#ab7291, 1/500; Abcam, Cat#ab18251, 1/1000). Coverslips were incubated with primary antibodies diluted in blocking buffer overnight at 4℃ and wash buffer used after antibody incubation was PBS containing 0.1% (v/v ...
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bioRxiv - Cell Biology 2020Quote: ... anti-HA (Santa Cruz, Cat#sc-7392, 1/100; Abcam, Cat#ab9134, 1/1000) and anti-α-Tubulin (Abcam ...
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bioRxiv - Neuroscience 2021Quote: ... incubated with antibody (Drosha, Cell Signaling, 1:50 or Safb1, Abcam 1:50 resp.) on a rotating wheel overnight at 4°C (where 2.5% of each sample had been saved as input) ...
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bioRxiv - Developmental Biology 2022Quote: Antibodies for immunofluorescence: Endomucin (1:400, R&D AF4666 or 1:300, Abcam ab106100), TER119 (1:300 ...
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bioRxiv - Cell Biology 2022Quote: ... rat anti-ATE1 (homemade, 1:1000) or mouse anti-GAPDH (Ab8245 Abcam, 1:5000).
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bioRxiv - Genomics 2020Quote: ... Primary antibodies used were mouse anti-FOXA1 (1:100 or 1:1000 dilution, Abcam); goat anti-FOXA2 (1:300 dilution ...
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bioRxiv - Developmental Biology 2019Quote: ... mouse anti-Dlg (1:250, DSHB) and FITC-conjugated anti-GFP (1:400, Abcam).
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bioRxiv - Genetics 2019Quote: ... Primary antibodies include rat anti-Deadpan (Abcam Cat# ab195172, 1:250 or 1:500), mouse anti-Prospero MR1A (Developmental Studies Hybridoma Bank ...
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bioRxiv - Neuroscience 2020Quote: ... GFP (1:2000, goat directly conjugated to FITC, Abcam; 1:10000, chicken, Aves Labs), PDGFRβ (1:200 ...
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bioRxiv - Neuroscience 2020Quote: ... ionized calcium-binding adapter molecule 1 - Iba1 (1: 1000; goat polyclonal; ab178846, Abcam, France); G alpha s/olf - Golf (1:500 ...
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bioRxiv - Pathology 2021Quote: ... anti-Catalase (R&D, AF3398, 1:50) and Ki-67 (abcam, ab16667, 1:200). Secondary antibodies were anti-rabbit IgG-HRP (Santa Cruz Biotechnology ...
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bioRxiv - Neuroscience 2020Quote: ... rat anti-DAT (1:5000; Chemicon) and guinea-pig anti-VGluT1 (1:4000, Abcam), mouse anti-HA (1:500 ...
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bioRxiv - Molecular Biology 2020Quote: ... RanBP2 (mouse monoclonal mAb414 1:5000, Cederlane, or rabbit polyclonal, 1:1000 dilution, Abcam), Ubc9 (rabbit polyclonal ...
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bioRxiv - Microbiology 2021Quote: ... dehydrated and incubated with an anti-GLP-1 antibody (1:800; Abcam, Cambridge, UK) overnight at 4°C after antigen retrieval and blocking for 1 hour ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were stained with antibodies against -Tubulin (Abcam; ab6160; 1:800 or 1:500), Cyclin B1 (Abcam ...