Labshake search
Citations for abcam :
1351 - 1400 of 10000+ citations for 1 2 4 4 4 Pentachloropentafluorobutane since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Plant Biology 2022Quote: ... After three washes with 1 x PBS the samples were incubated for 4 hours with anti-mouse secondary antibodies conjugated with Alexa Fluor 488 (ab150077, Abcam, UK) diluted 1:400 in PBS ...
-
bioRxiv - Genetics 2022Quote: ... Around 20 μg or 10 μg of chromatin were incubated overnight at 4°C with 10 μg H3.X/Y or 2.5 μg H3K9me3 antibody respectively (Active Motif-61161, Abcam-ab8898). Chromatinantibody extracted with incubated with protein G Dynal beads (Thermo Fisher Scientific ...
-
bioRxiv - Biochemistry 2023Quote: ... Pellets were washed and resuspended in PBS and subsequently incubated with 4 μg/ml of PE-conjugated GABARAP/GABARAPL1/GABARAPL2 antibody (Abcam, ab223948) for 1 h ...
-
bioRxiv - Cancer Biology 2023Quote: ... The resulting chromatin was incubated overnight at 4°C with histone modification antibodies (H3K27ac, Abcam ab4729; H3K27me3, Diagenode C15410195 and H3, Abcam ab1791) pre-conjugated with Dynabeads Protein G ...
-
bioRxiv - Biochemistry 2023Quote: ... Denatured samples were resolved in 4-12% Bis-Tris SDS-PAGE gradient gels and stained with InstantBlue® Coomassie protein stain (Cat#ab119211, abcam). Protein bands were excised from gel ...
-
bioRxiv - Cell Biology 2023Quote: ... Primary antibodies were diluted in blocking reagent and incubated overnight at 4 °C (Collagen I (# ab34710, Abcam, Collagen IV (#ab6586, Abcam), MUC-1 (MA1-06503 ...
-
bioRxiv - Cancer Biology 2023Quote: ... Membranes were incubated overnight at 4°C with primary antibody (VRK3 #HPA056489 Sigma, B-actine #51255 Cell signaling, Lamin # ab16048 Abcam, H3S10P #05-806 Millipore ...
-
bioRxiv - Immunology 2022Quote: ... For the intracellular S.Tm-LPS staining using α-S.Tm LPS (O-antigen group B factor 4-5, Difco) and α-rabbit-AlexaFluor647 (Abcam Biochemicals), cells were incubated with IC fixation buffer (eBioscience ...
-
bioRxiv - Molecular Biology 2023Quote: ... The membrane was incubated overnight at 4°C with primary antibodies phospho-STAT3-Tyr705 (Cell Signalling, 9145S), STAT3 (Cell Signalling, 4904S) and Twist1 (Abcam, ab50887) raised in rabbit ...
-
bioRxiv - Cell Biology 2024Quote: ... Primary antibodies were diluted in 5% BSA and incubated at 4°C overnight at given concentrations: α-smooth muscle actin (αSMA, mouse, Cat. No. ab7817, Abcam) at 1:300 dilution ...
-
bioRxiv - Biophysics 2024Quote: ... proteins were transferred to a nitrocellulose membrane (Li-Cor, 926-31092) and incubated overnight at 4°C with primary antibodies: Mic60/mitofilin (Abcam, ab110329). The membrane was incubated for 1 hour at room temperature with secondary antibodies ...
-
bioRxiv - Bioengineering 2024Quote: ... to deionized water and fixed in 4% PFA for 15 min before staining with picrosirius red according to the manufacturer’s instructions (Abcam, cat#: ab150681). Immunostaining ...
-
bioRxiv - Physiology 2024Quote: ... Protein was then transferred to a nitrocellulose membrane (Li-Cor 926-31092) that was incubated with primary antibodies overnight at 4°C: Mic60/mitofilin (Abcam ab110329), SAM50 (Proteintech 20824-1-AP) ...
-
bioRxiv - Biochemistry 2024Quote: ... Membranes were incubated for 2h (at RT) or 24h (at 4°C) with anti-6X His-tag antibody (Abcam, Cat#ab18184) or anti-CLN5 antibody (Abcam ...
-
bioRxiv - Biochemistry 2024Quote: ... Cells were transduced with the Sh2 or Scr control constructs on 4 DIV and cellular ATP measurements were performed on 18 DIV using a Luminescent ATP Detection Assay Kit (Abcam, ab113849). To block luminescence signal contamination from adjacent wells ...
-
bioRxiv - Genomics 2024Quote: ... 16.7 mM Tris pH 8 and 167 mM NaCl) and incubated overnight with an H3K27ac antibody with rotation at 4 °C (Abcam, ab4729). Protein G Dynabeads (Thermo Scientific ...
-
bioRxiv - Biophysics 2024Quote: ... Nuclear pore complex protein Nup133 or β-tubulin were labeled overnight at 4°C with rabbit anti-Nup133 antibody (Abcam, #ab155990) and mouse anti-human β-tubulin mAb (Sigma-Aldrich ...
-
bioRxiv - Cell Biology 2024Quote: ... followed by an overnight incubation at 4°C with Rabbit recombinant monoclonal anti-mouse Collagen type I antibody [EPR24331-53] (Abcam, ab270993), dissolved in the blocking solution (1:1000 dilution) ...
-
bioRxiv - Biochemistry 2024Quote: ... Eluted proteins were resolved on NuPAGE™ 4–12% Bis–Tris gel followed by Western blotting using anti-IFNAR1 antibody (ab124764, Abcam).
-
bioRxiv - Cancer Biology 2024Quote: ... 4 µm-thick TMA slides were incubated with the primary CD14 monoclonal antibody (clone 1H5D8, Abcam, Cat# ab181470, Camdridge, MA, USA) at a dilution of 1:3,000 for 15 minutes at room temperature ...
-
bioRxiv - Developmental Biology 2024Quote: ... in PBS for 1 h at RT and incubated with primary antibodies for at least 1 h at RT or overnight at 4°C (p300, Abcam, ab275388; CBP, Abcam, 253202; FLAG, Sigma, F1804; H3K27ac, Active Motif, 39133; H3K18ac, Abcam, ab40888 ...
-
bioRxiv - Developmental Biology 2024Quote: ... in PBS for 1 h at RT and incubated with primary antibodies for at least 1 h at RT or overnight at 4°C (p300, Abcam, ab275388; CBP, Abcam, 253202; FLAG, Sigma, F1804; H3K27ac, Active Motif, 39133; H3K18ac, Abcam, ab40888; H2BK5ac, Abcam, ab40886 ...
-
bioRxiv - Immunology 2021Quote: ... then were fixed with 4% paraformaldehyde (PFA) for 15 min at room temperature and stained using the Senescence-Associated β-Galactosidase Staining Kit (Abcam ab65351), following the manufacturer’s instructions ...
-
bioRxiv - Biochemistry 2020Quote: ... After blocking with 5% defatted milk, membranes were incubated (overnight, 4°C) with primary antibodies (anti-NNT cat. Abcam: ab110352; β-actin cat. Abcam ab8226). Membranes were then incubated with secondary antibodies conjugated to fluorescent (Licor® ...
-
bioRxiv - Neuroscience 2021Quote: ... Sections were incubated overnight at 4 °C with primary antibodies prepared in blocking buffer against phospho-MLKL (Ser345) (Abcam, Cat. No. ab196436), MLKL (Millipore ...
-
bioRxiv - Physiology 2020Quote: ... Cells were washed 3 × in TBS and incubated overnight (4°C) in 300 μl of anti-desmin (Abcam Cat# ab15200, RRID: AB_301744) primary antibody made up in TBS ...
-
bioRxiv - Molecular Biology 2022Quote: ... Protein was adjusted to 400 µg and incubated O/N at 4℃ with 5 µg of IP-antibody (Table S1) or rabbit IgG control (Abcam, Cambridge, UK) in a final volume of 500 µl of NT2 buffer (150 mM NaCl ...
-
bioRxiv - Immunology 2020Quote: ... The sonicated nuclear fractions were divided for input control and for overnight incubation at 4℃ with 10 μg of either anti-C/EBPβ (Abcam cat. 32358) or the negative control IgG ...
-
bioRxiv - Physiology 2022Quote: ... 4 µm serial sections of paraffin-embedded slides were incubated with rat anti-mouse CD34 (MEC 14.7, monoclonal, Abcam, Cambridge, United Kingdom) at a 1:200 dilution overnight at 4° ...
-
bioRxiv - Genetics 2022Quote: ... After 6 h the cells were washed in 1XPBS and fixed in 4% PFA followed by staining with rabbit polyclonal beta COP antibody (ab2899, Abcam, Cambridge, UK) after permeabilising with 0.5% Triton-X 100 ...
-
bioRxiv - Immunology 2022Quote: Binding of TIM-4 to infected cells was tested using a TIM-4 Fc chimera composed of the extracellular domain of TIM-4 fused to the N-terminus of the Fc region of human IgG (Abcam, Waltham, MA). One μg of TIM-4 in PBS containing 1% BSA was incubated on ice for 30 minutes with 105 washed CD4 T cells infected as indicated above with SIVGAGGFP ...
-
bioRxiv - Immunology 2022Quote: ... for 30 minutes at 4°C and then incubated with the titrated primary anti-MPO-PE and primary citrullinated histone H3 (citH3) antibody (Abcam, Waltham, MA) for 30 minutes at 4° C ...
-
bioRxiv - Physiology 2022Quote: ... Membranes were incubated in the following primary antibodies overnight at 4°C: polyclonal rabbit anti-beta actin IgG (Abcam Cat# ab8227, RRID:AB_2305186) at a dilution of 1:1,000 ...
-
bioRxiv - Cell Biology 2021Quote: Cell supernatant was collected 24h after changing cell medium then immediately filtered by centrifugation for 15 minutes at 4°C on a 10KDa column (Abcam or Millipore). This step eliminates proteins ...
-
bioRxiv - Physiology 2022Quote: ... washed with PBS-T 3 times for 10min and incubated O/N at 4°C with primary antibody (Rabbit anti-Calnexin, Abcam, Cat# ab133615) at a dilution of 1:1000 in PBS-T ...
-
bioRxiv - Cell Biology 2022Quote: ... Sections were then incubated in the following primary antisera overnight at 4°C in PBT: guinea pig antiinsulin (Abcam; Waltham, MA, USA), rabbit anti-glucagon (Santa Cruz ...
-
bioRxiv - Pathology 2024Quote: ... Membranes were then blocked for 1 hour with 5% dry milk in PBST (PBS supplemented with 0.1% Tween-20) buffer and incubated overnight at 4°C with primary antibody (anti-ACE2, Abcam; anti-Actin, Abcam) diluted in PBST containing 1% BSA (Sigma) ...
-
bioRxiv - Molecular Biology 2023Quote: ... Cells were fixed in 4% paraformaldehyde for 15 min and stained with anti-γ-H2AX (CST,9718) and chicken anti-GFP (Abcam, #ab13970) primary antibodies in 1:400 and 1:600 dilution ...
-
bioRxiv - Microbiology 2023Quote: ... bound complexes were isolated by overnight precipitation of the pre-cleared chromatin with 100 µL of blocked beads in combination with 3-4 µg of antibodies (anti-Histone H3 (Abcam ab17913, µg), Histone H3Ac (Millipore 17-615 ...
-
Molecular Insights into the Recognition of Acetylated Histone Modifications by the BRPF2 BromodomainbioRxiv - Biochemistry 2022Quote: Pull-down experiments were performed by following the method previously described.36 15-20 μg of acid extracted histones or nucleosomes isolated from HeLa cells were mixed with 50 μM of recombinant His6-tagged-BRPF2 bromodomain incubated at 4°C on a nutator for 60 min in the presence or absence of 10 μM BAY- 299 (abcam cat# ab230368) inhibitor ...
-
bioRxiv - Cancer Biology 2022Quote: ... at room temperature for 1 hour and then incubated overnight at 4°C with the following primary antibodies: KLF4 (R&D, AF3640) and cyclophilin B (abcam, catalog ab16045). Chemiluminescent detection was performed with HRP-conjugated secondary antibodies (Santa Cruz Biotechnology Inc. ...
-
bioRxiv - Neuroscience 2022Quote: ... Cells were incubated overnight at 4 °C with primary antibodies — targeting α-syn (Mouse anti α-syn, AA 124-134, ab1903, Abcam; Mouse anti α-syn 4B12 ...
-
bioRxiv - Microbiology 2022Quote: ... Mitochondrial membrane potential was measured using a tetramethylrhodamine ethyl ester (TMRE) and carbonyl cyanide 4-(trifluoromethoxy) phenylhydrazone (FCCP) as a control (Abcam, Cambridge, UK). Prior to incubation and acquisition ...
-
bioRxiv - Cell Biology 2024Quote: ... in PBST for two hours followed by overnight incubation (4 °C) with anti-DNA/RNA damage antibody [15A3] (Abcam, cat. No. ab62623) at 1:2500 in blocking solution ...
-
bioRxiv - Biophysics 2024Quote: ... and then were blocked with the 10% normal goat serum for 45 minutes at room temperature before incubating with the primary antibodies (anti-γH2AX: Beyotime, no. C2035S-4; anti-pSTING: Cell Signaling Technology, no. 40818S; anti-cytochrome c and anti-complex V: Abcam, no ...
-
bioRxiv - Biochemistry 2024Quote: ... Immunoprecipitation of mGluR2 was performed overnight at 4°C using a mouse monoclonal anti-mGluR2 N-terminal antibody (Abcam, Catalog no. ab15672), followed by RNA isolation ...
-
bioRxiv - Neuroscience 2024Quote: ... and 4 μg of the specific antibody (rabbit anti-Rpb1, Cell Signaling, Cat.14958; rabbit anti-RNA Pol II phospho S2, Abcam, ab5095) was incubated in IP buffer (0,1% SDS ...
-
bioRxiv - Bioengineering 2024Quote: ... Extracellular glutamate was measured in effluents collected at the end of TNFα administration (day 4) using the same kit used for the glutamate measurements in the bicuculline experiment (Abcam, Cat.# ab83389). Chips were then fixed for immunostaining and confocal microscopy ...
-
bioRxiv - Cell Biology 2024Quote: ... The membrane was blocked with 5% skim milk in Tris-buffered saline containing 0.1% Tween 20 (TBS-T) and incubated overnight at 4℃ with primary antibodies against NDUFA9 (Abcam, Cat. No. ab14713), MTCO2 (ThermoFisher ...
-
bioRxiv - Cancer Biology 2024Quote: ... were diluted in blocking buffer and incubated on tissue sections at 4°C for 16h (Abcam, Cambridge, UK; Bioss USA, Woburn, MA). Tissue sections were washed with 0.05% Tween 20 in PBS ...