Labshake search
Citations for abcam :
1301 - 1350 of 10000+ citations for 1 2 4 4 4 Pentachloropentafluorobutane since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2024Quote: ... Pooled chromatin was incubated at 4°C for 12−14 h with rotation with 2.5 µg anti-H3K27ac (Abcam, ab4729) or 2.5 µg anti-H3K4me3 (Millipore ...
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bioRxiv - Neuroscience 2024Quote: ... Brain sections were post-fixed on slides with 4% paraformaldehyde before being permeabilized with 3% TritonX-100 and immunostained with IBA1 (AbCam), NeuN (ThermoFischer) ...
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bioRxiv - Neuroscience 2024Quote: ... Slices were incubated overnight at 4 °C in the blocking solution with primary antibody (Iba1: rabbit anti-Iba1, ab178846 Abcam, 1:200 dilution ...
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bioRxiv - Physiology 2024Quote: ... Colonic sections were then incubated with primary antibodies overnight at 4°C followed by secondary antibodies (mouse IgG ab150113 Abcam, rabbit IgG ab96891 Abcam) for 1 hour at room temperature ...
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bioRxiv - Molecular Biology 2024Quote: ... Paraffin-embedded tissue sections of 4 µm thick from the distal colon were processed following the manufacturer’s protocol for the TUNEL assay kit (Abcam, #ab206386). Images of 10 randomly selected fields per slide were captured at 400X magnification ...
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bioRxiv - Bioengineering 2024Quote: Levels of glucose and glutamine were determined via analysis of spent culture medium (n=4/group) using a Glucose Assay Kit and a Glutamine Assay Kit (Abcam), respectively ...
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bioRxiv - Cell Biology 2024Quote: ... followed by an overnight incubation at 4°C with primary antibodies specific for Osx and Cx43 both anti-rabbit (Abcam, Cambridge ...
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bioRxiv - Cell Biology 2024Quote: ... The following primary antibodies were used for cell staining overnight at 4°C: anti-alpha smooth muscle actin [1A4] antibody (Abcam) and isotype control mouse IgG2a (Sigma ...
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bioRxiv - Developmental Biology 2020Quote: ... Embryos were incubated in primary antibody overnight at 4° C (chick α-GFP, Abcam, Cat# AB13970, Lot# GF305729-1; mouse α- γ-tubulin, Sigma-Aldrich, Cat# T6557, Lot# 066M4858V; rabbit α- α- tubulin, Abcam, Cat# AB233661) and in secondary antibody for two hours at room temperature (Alexa goat α-chick 488 ...
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bioRxiv - Cancer Biology 2021Quote: ... carried out overnight on a rotating wheel at 4 °C in the presence of 20 μg of anti-V5 antibody (Abcam, ab9116). 2.5% of the input sample was collected just before addition of the antibody and stored at −20 °C ...
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bioRxiv - Cell Biology 2020Quote: ... the cells were incubated at 4°C with one of the following mouse monoclonal primary antibodies: anti-heavy chain cardiac myosin (Abcam, #ab50967), anti-α-actinin (Sigma-Aldrich ...
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bioRxiv - Developmental Biology 2020Quote: ... at 4 °C overnight followed by staining with a secondary goat-anti-rabbit antibody coupled with Alexa 568 (ab175471, Abcam, US).
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bioRxiv - Genomics 2020Quote: ... and immunoprecipitation was performed using 4 μg of the appropriate antibody (anti-CTCF: 61311, Active Motif; anti-RAD21: ab88572, Abcam; anti-GFP: ab290, Abcam). Paired-end sequencing was performed on a NovaSeq6000 platform (Illumina ...
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bioRxiv - Neuroscience 2020Quote: ... Sections from animals not injected with FITC-Dextran were next incubated under stirring for 48 hours at 4°C in PB containing rabbit polyclonal anti-collagen IV antibody (Abcam, Ab6586) at a 1:500 dilution ...
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bioRxiv - Neuroscience 2020Quote: ... Samples were resolved by SDS-PAGE using a 4-15% gradient gel and analyzed by Western blot using the following primary antibodies: rabbit anti-GFP (Abcam Ab290), rabbit anti-Cx34.1 3A4-conjugated-680LT ...
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bioRxiv - Microbiology 2021Quote: ... then incubated overnight at 4°C with the primary antibody mouse anti-SA Hla [8B7] -N-terminal (Abcam, Cambridge, United Kingdom) diluted 1:2500 in the blocking buffer ...
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bioRxiv - Cancer Biology 2020Quote: ... 1mL of Chromatin was incubated 12-16 hours at 4°C with 10μg of antibody (α-RNA Pol2 [sera from Gardini lab], α-Twist [Abcam ab50887] ...
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bioRxiv - Cell Biology 2021Quote: ... Afterwards membranes were blocked for 30 min with 5% Nutrilon (Nutricia) in PBS and incubated overnight at 4°C with primary antibodies H3 (Abcam 1791), H4 (Merck Millipore 04-858) ...
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bioRxiv - Bioengineering 2021Quote: ... cells were incubated overnight at 4 °C with recombinant anti-vimentin antibody conjugated with Alexa Fluor® 568 (ab202504, Abcam®) at a 1/1000 dilution ...
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bioRxiv - Cancer Biology 2021Quote: ... Blots were blocked for 1 hour at room temperature with 5% fat-free milk or milk-free blocking buffer (for analysis of phosphorylated proteins) and incubated overnight at 4°C with primary antibodies for p-GCN2 (Abcam, ab75836), p-eIF2α (Cell Signaling ...
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bioRxiv - Cell Biology 2021Quote: ... slides were thoroughly washed using PBS 1X/Triton- X 0.1% and incubated overnight at 4°C with the respective antibodies anti-mouse Alexa-Fluor® 594 (abcam, ab150108), anti-sheep Alexa-Fluor® 488 (Jackson Immunoresearch ...
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bioRxiv - Immunology 2022Quote: ... and CTLA-4 Ig (Abatacept) were generated using the APC Conjugation Kit - Lightning-Link® according to manufacturer’s instructions (Abcam, ab201807). APC conjugated anti-PD-L1 antibodies were procured from Biolegend (clones MIH1 ...
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bioRxiv - Microbiology 2020Quote: ... endogenous human NPC1 and HSP90 were purified using immobilized Recombinant Protein G Resin (Generon) and 4 µg of specific antibodies against NPC1 (Abcam, ab108921) or HSP90 (Enzo Life Sciences ...
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bioRxiv - Neuroscience 2021Quote: ... 0.1% SDS) and incubated overnight at 4°C with 3 μL specific mouse monoclonal antibody recognizing Bcl11a (Abcam Cat# ab19487, RRID:AB_444947) or unspecific IgG1 antibody (Cell Signaling Technology Cat# 5415 ...
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bioRxiv - Neuroscience 2022Quote: ... Samples were resolved by SDS-PAGE using a 4–15% gradient gel and analyzed by Western blot using the following primary antibodies: chick anti-GFP (Abcam Ab13970), rabbit anti-Cx34.1 3A4-conjugated-680LT ...
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bioRxiv - Genetics 2020Quote: ... the membranes were then probed at 4°C overnight with various primary antibodies: anti-foxa2 (Seven Hills) or LXR-α (Abcam), washed with TBST (20 mM Tris ...
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bioRxiv - Physiology 2020Quote: ... Sections were incubated overnight in a humidity chamber at 4°C in a mixture of rabbit anti-androgen receptor (Spring Bioscience m4070) and chicken anti-tyrosine hydroxylase (Abcam ab76442) in NGS/TBS/BSA ...
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bioRxiv - Biophysics 2020Quote: ... A negative control was performed on a subset of the wells by treating cells with a spike of 10 µM Carbonyl cyanide 4-(trifluoromethoxy)phenylhydrazone (FCCP, Abcam ab120081) 10 minutes prior to the application of TMRE ...
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bioRxiv - Microbiology 2023Quote: ... Lysates were incubated with antibodies for one hour at 4°C in an overhead tumbler before immune complexes were retrieved by protein G (GE Healthcare or abcam) or A Sepharose (GE Healthcare) ...
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bioRxiv - Neuroscience 2023Quote: ... After SDS-PAGE and proteins transferred on PVDF membranes were incubated overnight at 4°C with Total OXPHOS Human WB Antibody Cocktail (Abcam, UK) and subsequently detected with HRP-conjugated goat anti-rabbit IgG secondary antibody (1:4000 ...
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bioRxiv - Neuroscience 2023Quote: ... The sections were then incubated overnight at 4°C in primary antibody diluted in blocking solution with 5% normal goat serum (Abcam, ab7481). Antibodies were directed against GFP (Abcam ...
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bioRxiv - Biochemistry 2023Quote: Rat sera were incubated at 4°C for 16 h in the presence of anti-adiponectin antibodies from Abcam (ab 56416) and Cell Signaling (2789s) ...
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bioRxiv - Cancer Biology 2023Quote: ... Samples were pre-cleared using the beads provided in the kit and incubated overnight at 4°C with 5 µg of commercially available antibodies against lamin A/C (Abcam, #ab108595), phospho-Ser301 lamin A/C (Affinity ...
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bioRxiv - Cancer Biology 2023Quote: ... Beads were removed and nuclear extracts were incubated overnight at 4°C with either anti-lamin A/C antibody (Abcam, #ab108595) or non-immune IgG ...
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bioRxiv - Cancer Biology 2023Quote: ... and sheared chromatin was incubated at 4°C overnight with 4µg of primary β-catenin (CST, D10A8) or SOX2 (Abcam, ab97959), or isotype control antibodies (rabbit IgG ...
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bioRxiv - Physiology 2023Quote: ... Frozen sections were fixed with fresh 4% paraformaldehyde and neutrophil elastase protein was detected using anti-neutrophil elastase antibody (Abcam #68672).
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bioRxiv - Molecular Biology 2023Quote: ... which was performed overnight at 4°C by incubating the shared DNA-protein complex with 10 µg of anti-ADNP (Abcam Ab231950) antibody ...
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bioRxiv - Systems Biology 2023Quote: ... Primary antibodies were diluted in 5% milk TBST and incubated with blots overnight at 4 °C (FGF1 – Abcam, ab207321; β-ACTIN – Abcam, ab8226 ...
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bioRxiv - Molecular Biology 2023Quote: ... in methanol and non-specific binding blocked with 10% serum from the source species for the secondary antibody followed by a primary antibody incubation overnight at 4 °C (Sox9, abcam, ab185966,; p16, (abcam, ab241543), α-SMA (Sigma-Aldrich’ A5691) ...
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bioRxiv - Cell Biology 2022Quote: ... the membrane was blocked using the Odyssey blocking buffer (LiCor) and incubated overnight at 4 °C with the primary antibody (SHIP2 antibody (Abcam #ab70267) 1/2000 ...
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bioRxiv - Genetics 2023Quote: ... An aliquot of total fragmented chromatin was saved as an input control and the remaining chromatin was split into two tubes (representing 5×107 cells each) for antibody pulldown and incubated overnight at 4°C with 15 ug of NRF1 (Abcam #ab55744), or MYC (Abcam #ab56 ...
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bioRxiv - Biochemistry 2023Quote: ... Fractions were analyzed by separation using sodium dodecyl sulfate polyacrylamide gel electrophoresis (4–12% polyacrylamide) and staining with InstantBlue Coomassie Protein Stain (Abcam, UK). Fractions containing chalcone synthase were combined and loaded onto a HiLoad 16/600 Superdex 200 pg column ...
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bioRxiv - Microbiology 2024Quote: ... which was then washed in PBS with 0.1% Tween-20 (PBS-T) and incubated overnight at 4°C shaking in PBS-T with rabbit anti-GAC antibodies (Abcam ab21034) at a 1:5,000 dilution and mouse anti-His monoclonal antibody (Thermo Fisher Scientific or Abcam ab216773 ...
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bioRxiv - Cancer Biology 2024Quote: ... 20 μL of GBO medium containing 1 mM ACh with or without a combination of the M3 muscarinic receptor antagonist 4-DAMP (100 μM) and pan-nicotinic receptor antagonist mecamylamine (100 μM, Abcam, ab120459) was added to each GBO at the ALI surface 30 minutes prior to imaging with the same conditions ...
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bioRxiv - Cancer Biology 2023Quote: ... Then cells were washed three times with IF buffer and incubated overnight at 4°C with the following antibodies : 53BP1 (abcam, ab36823) or Rabbit IgG Isotype Control (CellSignaling ...
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bioRxiv - Neuroscience 2023Quote: ... Tissue was then incubated overnight at 4 °C on a shaker in primary antibodies against PGP9.5 (from rabbit; Abcam, catalog # EPR4118) and tdTomato (from goat ...
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bioRxiv - Cell Biology 2023Quote: ... This viral supernatant was then concentrated by centrifugation at 3200 x g for 30 minutes at 4°C before being resuspended in 100 µl virus suspension solution (Abcam, ab102538), aliquoted and stored at -70°C ...
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bioRxiv - Developmental Biology 2022Quote: ... Membrane was blocked for one hour in 5% of milk or TBS-T and incubated overnight at 4°C with anti-vinculin (Abcam 73412), anti-CREB (Santa Cruz SC-186) ...
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bioRxiv - Cell Biology 2022Quote: Grown BEC-organoids were treated with the FA-mix for 4 days and triglyceride levels were measured with a Triglyceride kit (Abcam, ab65336) following the manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: ... The slides were incubated with primary rabbit anti-SARS nucleocapsid protein antibody(NB100-56576, Novus, USA) overnight at 4°C and then with goat anti-rabbit IgG HRP (Abcam, UK) for 1 hr ...