Labshake search
Citations for abcam :
1251 - 1300 of 10000+ citations for 1 2 4 4 4 Pentachloropentafluorobutane since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: ... 500 µg of total protein extract were used to IP with either 2.5 µg V5 rabbit polyclonal antibody or VPS35 mouse monoclonal over night at 4°C and recovered after incubating for 6h at 4°C in a rotator with equilibrated Agarose Protein-G beads (Abcam). Immunoprecipitation followed by MS analysis was performed as described but using magnetic Protein G beads (Thermo Scientific) ...
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bioRxiv - Neuroscience 2021Quote: ... The following day the slices were washed three times for 10 minutes in PBS and incubated with secondary antibodies overnight at 4°C (Goat-anti-Guinea-pig-Alexa Fluor 488, Abcam ab150185 ...
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bioRxiv - Genomics 2020Quote: ... The sonicated chromatin had a size in the range of 100–500 bp and was incubated overnight at 4°C with the anti-H3K27ac antibody (#ab4729, Abcam). Samples were incubated for 1h at 4°C with Dynabeads Protein G for Immunoprecipitation (#10003D ...
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bioRxiv - Cell Biology 2021Quote: ... The sections were blocked in normal goat serum for 30 minutes and incubated overnight at 4°C with the primary antibodies against TCF1 (Ab96777, Abcam; 10 µg/ml for knee ...
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bioRxiv - Physiology 2022Quote: 10 µg of protein from 4-week-old adrenals were used for measurement with PKA kinase activity kit (ab139435, Abcam).
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bioRxiv - Cell Biology 2022Quote: ... paraffin slices were blocked for 1 hour at 37°C with 5% BSA before being treated overnight at 4°C with various primary antibodies as follows: anti-GFAP (Abcam), anti-CD68 (Abcam) ...
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bioRxiv - Molecular Biology 2022Quote: ... Chromatin fractions of 400 μl were taken for each immunoprecipitation reaction and incubated with 4 μl of anti-histone H3 antibodies (ab1791, Abcam) at 4°C overnight ...
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bioRxiv - Cancer Biology 2022Quote: ... The remaining soluble chromatin was incubated overnight at 4°C with 3μg of either c-Myc antibody (anti-c-Myc, Abcam, ab56) or mouse IgG antibody (normal mouse IgG ...
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bioRxiv - Developmental Biology 2022Quote: ... 100µg of cleared chromatin (protein) was incubated with 2µg of the following antibodies overnight at 4°C: H3K27ac (Abcam, ab4729), H3K4me1 (Abcam ...
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bioRxiv - Biophysics 2020Quote: ... then incubated to 37°C for 30 min in the dark with 4 µg/ml of Pyronin Y (abcam, ab146350), diluted in FACS buffer ...
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bioRxiv - Cancer Biology 2021Quote: ... Sections were incubated overnight at 4°C with isotype controls and primary antibodies against human CXCL5 and human CXCL8 (Abcam), then detected with biotinylated secondary antibodies (Vector ...
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bioRxiv - Molecular Biology 2020Quote: ... Primary antibody incubation was overnight at 4°C (anti-RIF1, Bethyl Laboratories A300-568A; anti-MCM4, Abcam ab4459; anti-GFP, Abcam ab290 ...
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bioRxiv - Microbiology 2021Quote: ... Cells were permeabilized with 0.1% Triton X-100 in PBS for 10 min at RT and stained overnight at 4°C with the appropriate primary antibodies (rabbit anti-ZMPSTE24, Abcam ab38450 ...
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bioRxiv - Immunology 2022Quote: ... permeabilized with 0.1% Triton X-100 in PBS and stained overnight at 4°C with ACE2 protein-specific antibody (Abcam Ab15348). Cells were then incubated with anti-rabbit secondary antibody (Alexa Fluor 536 anti-rabbit ...
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bioRxiv - Cell Biology 2022Quote: ... and incubated overnight at 4°C with primary antibodies diluted in Intercept 0.05% Tween-20 as follows: anti-ATP5A1 (Abcam; ab14748), anti-Citrate synthase (Abcam ...
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bioRxiv - Biochemistry 2020Quote: ... Subsequently they were incubated overnight at 4 °C with the primary antibodies against pT72 (MJF-R20, abcam, Cat. No. ab231706) and the His-tag of Rab8a (anti-His-Antibody ...
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bioRxiv - Cancer Biology 2020Quote: ... 50 μL of sheared chromatin was reserved as input and ChIP performed overnight at 4°C with rotation with 7.5 μg of antibody per IP: H3K27Ac (Abcam ab4729), BRD4 (Bethyl Laboratories A301-985A100).
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bioRxiv - Molecular Biology 2021Quote: ... Sample buffer was added to the reaction and incubated at 37° for 20 minutes before SDS-PAGE and Western blot analysis using antibody MM13-4 (AbCAM). For each transfected construct ...
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bioRxiv - Cell Biology 2020Quote: ... The samples were washed with PBS and then incubated with biotinylated anti-mouse IgG secondary antibody (4 µg/ml, abcam) and Alexa Fluor 488 conjugated anti-rabbit IgG secondary antibody (1:250 ...
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bioRxiv - Cancer Biology 2021Quote: ... Lysates were cleared by centrifugation (14,800 g, 20 min, 4°C) and subsequently incubated with anti-HOXA5 antibody (Abcam, #ab82645), anti-DDK (FLAG ...
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bioRxiv - Physiology 2021Quote: ... Sections were blocked with 5% donkey serum in the presence of 0.1% Triton followed by overnight incubation with primary antisera at 4 degrees (pCREB, Cell Signaling; Glucagon, Abcam). After extensive washing ...
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bioRxiv - Cancer Biology 2021Quote: ... were incubated at 4°C for at least 18 h with either anti-RBM3 monoclonal antibody (Abcam, Cambridge, United Kingdom) or anti-rabbit-IgG-antibody (Cell Signaling ...
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bioRxiv - Neuroscience 2021Quote: ... peptide C-AGQERFH(pT)ITTSYYR (conjugated with either –KLH or -OVA) was used to inject 4 rabbits (New Zealand White rabbits) by Abcam. Rabbit bleeds/antisera were collected and screened by peptide ELISA assay and western blot ...
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bioRxiv - Immunology 2020Quote: ... Immunoprecipitation was performed overnight at 4°C with 2μg of primary antibody (p65: Santa Cruz sc-372, H3K27ac: Abcam ab4729), or corresponding species-appropriate IgG control antibody (GenScript) ...
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bioRxiv - Developmental Biology 2022Quote: ... peak incident power 105 W) and incubated overnight at 4° with magnetic beads conjugated to the H3K27ac antibody (Abcam, ab4729). The next day ...
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bioRxiv - Neuroscience 2022Quote: ... for 30 min followed by overnight incubation at 4° in primary antibodies, Aβ (Novus, NBP11 -97929) and NRN1 (Abcam, ab64186). Membranes were washed with 1×Tris-buffered saline containing 0.1% Tween 20 (TBS-T ...
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bioRxiv - Physiology 2022Quote: ... permeabilized and saturated for 30 minutes at room temperature in PBS with 0.01% Triton-X PBS and 4% donkey serum and incubated with chicken anti-GFAP (Abcam, ab4674) and rabbit anti-S100β (Dako ...
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bioRxiv - Microbiology 2024Quote: ... Sections were then washed in deionized water and blocked with a solution of 5% normal goat serum (NGS) in PBS for 15 min at 37 °C and then stained overnight at 4 °C with 4G2 antibody (Abcam) at 1:200 ...
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bioRxiv - Molecular Biology 2024Quote: Cells were stained 3-4 days after editing with anti-Cytokeratin 5 antibody [EP1601Y] (Alexa Fluor® 647) (Abcam, ab193895), Rabbit IgG ...
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bioRxiv - Molecular Biology 2024Quote: ... Debris were removed using centrifugation and the chromatin was incubated overnight at 4°C with antibody against H3K9me3 (Abcam, Ab8898). Antibody-bound chromatin was captured using magnetic beads (Themo Fisher ...
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bioRxiv - Neuroscience 2024Quote: ... 0.1% BSA pH 7.4 in phosphate buffered saline (PBS) or Tris-HCl buffered saline (TBS) and incubated at 4°C overnight with primary antibodies (anti-Nr4a2, Abcam, ab41917 ...
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bioRxiv - Cancer Biology 2023Quote: ... were sonicated and incubated overnight under rotation at 4 °C with 5 ug/ml of anti-RNAPII-Ser2p (Abcam, ab193468) or anti-IgG (Cell Signaling ...
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bioRxiv - Immunology 2023Quote: ... Tissues stained with CD163 were then subject to antigen retrieval using sodium citrate pH6 and incubated overnight at 4°C with rabbit anti-IBA1 (ab178846, abcam), followed by anti-rabbit AF488 secondary antibody or with rabbit anti-IBA1 AF647 (ab225261 ...
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bioRxiv - Physiology 2023Quote: ... Tissue sections were incubated overnight at 4°C with a cocktail diluted in Buffer W (Nanostring; Seattle WA) containing morphology markers Desmin (Abcam, U.K ...
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bioRxiv - Cell Biology 2023Quote: ... were incubated for 6 hours on a rotating wheel at 4°C with primary antibodies MTHFD2 (Abcam; #ab151447; 5 μg) or negative control IgG (Sigma-Aldrich ...
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bioRxiv - Developmental Biology 2023Quote: ... the samples were washed with several changes of PBS over minimum of 4 h at room temperature and mounted on microscopy slides with Anti-Fade Fluorescense Mounting Medium (Abcam).
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bioRxiv - Molecular Biology 2023Quote: ... The membrane was blocked in 5% non-fat milk powder in PBS-T followed by overnight incubation at 4°C with primary antibodies (anti-GFP; Abcam ab290 1:1000 ...
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bioRxiv - Developmental Biology 2023Quote: ... recovered to new tubes using a magnet and incubated overnight rotating at 4°C with 6.7 µg (20 µg total) of anti-H3K4me3 (Abcam ab8580) antibody per sample ...
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bioRxiv - Neuroscience 2023Quote: ... 2% goat serum) for 1h and then incubated overnight at 4°C in blocking buffer containing anti-PSER129 antibody EP1536Y (Abcam) diluted 1:50,000 ...
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bioRxiv - Immunology 2022Quote: ... Cells were then stained in blocking buffer overnight at 4°C with the following primary antibodies at the indicated dilutions: anti-Gal-9 (Abcam ab275877 ...
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bioRxiv - Immunology 2023Quote: The following primary antibodies were used for these experiments: rabbit anti-4 hydroxynonenal (HNE) antibody (10 µg/ml; ab46545, Abcam), rat anti-cd11b antibody (0.5 µg/ml ...
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bioRxiv - Cell Biology 2023Quote: ... and rabbit anti-titin M-line (Myomedix) primary antibodies overnight at 4°C then incubated in goat anti-mouse AF488 (Abcam) and goat anti-rabbit AF647 (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2023Quote: ... Primary antibodies were diluted in blocking reagent and incubated overnight at 4 °C (Collagen I (# ab34710, Abcam, Collagen IV (#ab6586, Abcam), MUC-1 (MA1-06503 ...
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bioRxiv - Cancer Biology 2023Quote: ... Where antibody staining was required coverslips were permeabilized with 0.2% Triton X-100 for 10 min and blocked in 4% BSA in PBS for 20 min followed by incubation with relevant antibodies (DHRS7 abcam at 1:1,000 dilution or rabbit lamin A antibodies [17] at 1:50 dilution for 1 hr at RT ...
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bioRxiv - Cancer Biology 2023Quote: ... The tissue sections were then stained overnight at 4°C with primary antibodies against anti-CD3 (ab52959, Abcam, CA, USA), anti-CD4 (AF-379-NA ...
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bioRxiv - Immunology 2023Quote: ... the specimens were incubated overnight at 4 °C with 5µg/mL rabbit anti-GFP antibody (ab290, Abcam, Cambridge, MA, USA). Subsequently ...
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bioRxiv - Cancer Biology 2023Quote: ... Following imaging for loading control the membrane was blocked in 5% BSA in TBS-T and incubated overnight at 4 °C using N6-methyladenosine antibody (ab151230, Abcam). The next day ...
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bioRxiv - Cancer Biology 2023Quote: ... Pyruvate concentration in the centrifuged extracellular medium samples (5 min 18000 g, 4°C) was measured in microplates using Pyruvate Assay kit (Abcam) or as described in 36.
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bioRxiv - Developmental Biology 2023Quote: ... Cells were then incubated overnight at 4°C on a shaker (65 rpm) with primary antibodies as follows: anti-CD206 (Abcam) for macrophages and anti-CD31 (Dako ...
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bioRxiv - Neuroscience 2024Quote: ... Brain sections were post-fixed on slides with 4% paraformaldehyde before being permeabilized with 3% TritonX-100 and immunostained with IBA1 (AbCam), NeuN (ThermoFischer) ...