Labshake search
Citations for abcam :
1301 - 1350 of 1481 citations for 5 tert Butyl 3 isocyanatoisoxazole since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cell Biology 2023Quote: ... were incubated for 6 hours on a rotating wheel at 4°C with primary antibodies MTHFD2 (Abcam; #ab151447; 5 μg) or negative control IgG (Sigma-Aldrich ...
-
bioRxiv - Molecular Biology 2023Quote: ... The membrane was blocked using TBS-T with 5% BSA for 1 hour at room temperature and incubated using p-SMAD3 (ab52903, Abcam), type I collagen (1310-30 ...
-
bioRxiv - Neuroscience 2023Quote: ... washed three times with PBS for 5 min each and incubated with secondary antibody Alexa Fluor® 647 (Abcam, ab150067) for an hour at room temperature ...
-
bioRxiv - Cancer Biology 2022Quote: ... Membranes were blocked with 5% milk/TBST at room temperature for 1 hr before being incubated overnight at 4°C with paxillin (Abcam) and GAPDH (Cell Signaling Technology ...
-
bioRxiv - Pharmacology and Toxicology 2023Quote: ... 5 μL of Annexin V-FITC and 5 μL of propidium iodide were added into each tube (Annexin V-FITC Apoptosis Staining/ Detection Kit, Abcam). The tubes were incubated at RT for 5 min in the dark ...
-
bioRxiv - Cell Biology 2023Quote: ... and incubated at RT for 1hr in secondary antibody diluted in TBST-5% milk (1:4000 ab6721 Goat Anti-Rabbit IgG H&L HRP (Abcam), 1:4000 ab205719 Goat Anti-Mouse IgG H&L HRP (Abcam)) ...
-
bioRxiv - Cancer Biology 2023Quote: The 5-ethynyl-2’-deoxyuridine (EdU) incorporation assay was performed using the EdU Proliferation Assay Kit (iFluor 488) (ab219801, Abcam). Cells were seeded onto coverslips and incubated with 10 μM EdU for 4 h at 37°C in a humidified atmosphere with 5% CO2 ...
-
bioRxiv - Immunology 2023Quote: Paraffin sections (5 μm) from lung tissues were processed using standard protocols for immunohistochemistry (IHC) staining according to manufacturer’s instructions (Abcam: ab236468). Tissues were permeabilized by incubation in 0.4% Triton-X in TBS for 20 min ...
-
bioRxiv - Bioengineering 2023Quote: ... was used to separately conjugate an anti-tau antibody (Tau-5, AB_2721194) and anti-α-synuclein antibody (MJFR1, Abcam, ab138501) to dibenzocyclooctyne (DIBO ...
-
bioRxiv - Molecular Biology 2023Quote: ... for 1 h prior to incubation in primary antibody in TBST with 5% skim-milk (rabbit anti-GFP 1:10,000; Abcam - ab290) overnight ...
-
bioRxiv - Pathology 2023Quote: ... Sections were washed 3 times for 5-10 min in PBT and incubated for 2 h at RT in secondary antibody and Hoechst (Abcam) in 3% NDS in PBT ...
-
bioRxiv - Cancer Biology 2023Quote: ... Following imaging for loading control the membrane was blocked in 5% BSA in TBS-T and incubated overnight at 4 °C using N6-methyladenosine antibody (ab151230, Abcam). The next day ...
-
bioRxiv - Cancer Biology 2023Quote: ... Antigen retrieval was accomplished by a 5-minute pressure cook of the slide submerged in 1X HIER buffer (abcam ab208572). Deionized water was slowly added to the pressure-cooked slide in HIER buffer ...
-
bioRxiv - Cell Biology 2023Quote: ... sections were blocked with 10% goat serum and then stained with Keratin 5 or Keratin 8 together with GFP antibody (Abcam) in 10% goat serum for overnight at 4□ ...
-
bioRxiv - Cancer Biology 2023Quote: ... Pyruvate concentration in the centrifuged extracellular medium samples (5 min 18000 g, 4°C) was measured in microplates using Pyruvate Assay kit (Abcam) or as described in 36.
-
bioRxiv - Neuroscience 2023Quote: ... The aCSF included 2,3-Dihydroxy-6-nitro-7-sulfamoyl-benzo(f)quinoxaline (NBQX, Alomone Labs) 20 μM and 2-amino-5-phosphonopentanoate (D-AP5, Abcam) 50 μM to block glutamatergic AMPA and NMDA currents ...
-
bioRxiv - Neuroscience 2023Quote: ... Sections were blocked with 5% donkey serum in 1x PBS for 1 hour and incubated with UFM1 (Abcam 1:100) and MC1 (1:1000 ...
-
bioRxiv - Molecular Biology 2024Quote: ... Wells were again washed three times for 5 minutes with dPBS before incubating with secondary antibodies (goat anti-rabbit IgG Alexa Fluor 488 [Abcam] ...
-
bioRxiv - Cell Biology 2024Quote: ... The membranes were blocked with 5% milk followed by incubation with primary antibodies overnight using the following antibodies: anti-VEGF-A (Abcam), anti-BCL2 (Cell Signaling Technology) ...
-
bioRxiv - Cancer Biology 2024Quote: ... or with recombinant TGF-β at 10 ng/mL (stock concentration 5 ng/µL) or Batimastat at 30 µM (stock concentration 1 mM, BB-94/Abcam). After 48 hours (or 1 hour for SMAD activity tests) ...
-
bioRxiv - Cell Biology 2024Quote: ... was detected in A549 cells fixed with 1% paraformaldehyde for 5 min using an anti-MT1-MMP rabbit monoclonal antibody (1:100 dilution, EP1264Y, Abcam). F-actin was stained with Alexa Fluor 350- or 568-conjugated phalloidin (Molecular Probes ...
-
bioRxiv - Microbiology 2024Quote: ... Serine racemase treatment of human serum was performed with the addition of 5 µl of proprietary serine racemase solution from a DL-serine assay kit (Abcam) to 1 ml of serum for 3 h ...
-
bioRxiv - Neuroscience 2024Quote: ... The membrane was blocked with 5% skimmed milk and probed with MJFR-14-6-4-2 (Abcam, 1:3000 dilution) and EP1536Y (Abcam ...
-
bioRxiv - Physiology 2024Quote: ... Sections were placed in 100% propylene glycol for 5 minutes then incubated in pre-heated Oil Red O (Abcam #150678) for 6 minutes at 60°C ...
-
bioRxiv - Microbiology 2024Quote: ... The membrane was incubated with the primary antibody (RPS3, abcam, Cat#ab128995; eIF4E, abcam, Cat#ab33768; ORF3a, abcam, Cat#280953; 5-mC, Cat#ab214727; IFN-beta, abcam, Cat#ab275580 ...
-
bioRxiv - Microbiology 2024Quote: ... unskimmed milk powder, and incubated with mouse monoclonal anti-HA (clone 6E2, Cell Signalling Technology) and anti-p24 (clone 5, Abcam) overnight at 4°C ...
-
bioRxiv - Microbiology 2024Quote: ... To test for O-x-AlkTDM presence we used in-gel fluorescence (to detect TAMRA) and/or immunoblotted with anti-biotin antibodies (1:1000; 5% nonfat Milk in TBST; Abcam).
-
bioRxiv - Molecular Biology 2024Quote: ... 3 µl of 1 mM DCFDA dye in 1X buffer and 5 µl of 10 µM DiOC2 was added for ROS evaluation (ab113851, Abcam) and membrane depolarization (B34950 ...
-
bioRxiv - Microbiology 2024Quote: Anti-HIV-1 Pr55Gag + p24 + p17 antibody (ab63917) and anti-tubulin antibodies (B-5-1-2) were purchased from Abcam and Sigma-Aldrich ...
-
bioRxiv - Neuroscience 2024Quote: ... cells were incubated with 20 μM NE for 5 min and an aliquot of saline was used to measure adenosine using an adenosine assay kit (Abcam) and a microplate reader (Perkin Elmer Enspire ...
-
bioRxiv - Molecular Biology 2024Quote: ... Blots were subsequently washed 3 times for 5 minutes each in PBST at room temperature before incubation in 5µg/mL of HNRNPA2B1 polyclonal primary antibody (abcam 31645) in PBST overnight at 4°C with agitation ...
-
Decreased Astrocytic CCL5 by MiR-324-5p Ameliorates Ischemic Stroke Injury via CCR5/ERK/CREB PathwaybioRxiv - Neuroscience 2024Quote: ... The PVDF membranes were blocked with 5% BSA and probed with primary antibodies (phospho-ERK1/2, Abcam, ab76299; ERK1/2, Abcam, ab184699 ...
-
Decreased Astrocytic CCL5 by MiR-324-5p Ameliorates Ischemic Stroke Injury via CCR5/ERK/CREB PathwaybioRxiv - Neuroscience 2024Quote: ... The PVDF membranes were blocked with 5% BSA and probed with primary antibodies (phospho-ERK1/2, Abcam, ab76299; ERK1/2, Abcam, ab184699; phospho-CREB, Abcam, ab32096 ...
-
bioRxiv - Immunology 2024Quote: ... Cells were permeabilized with 0.1% Triton X-100 / 2% BSA in 1x PBS for 5 min and F-actin was stained by Phalloidin-iFluor488 (1:1,000, Abcam #ab176753) for 1h room temperature prior to slides mounting (Fluoromount/DAPI).
-
bioRxiv - Cancer Biology 2024Quote: ... All of supernatant containing digested nuclear extract was transferred to a new tube and 500µl of Low IP buffer without additional NaCl or detergent was added together with 5-10µg of KAT7 antibody (Abcam #190908) or rabbit IgG (R&D Systems ...
-
bioRxiv - Physiology 2024Quote: ... The membranes were blocked (5% milk (PanReac AppliChem, A0830)) and probed with the following antibodies: anti-β-Tubulin (Abcam, ab15568), anti-GAPDH (Proteintech ...
-
bioRxiv - Immunology 2024Quote: ... ∼50 µg of lysate material was treated with the reducing agent TCEP at a final concentration of 5 mM and heated to 60 °C for 30 minutes before being loaded onto a FASP protein digestion column (Abcam)75 ...
-
bioRxiv - Bioengineering 2024Quote: ... the blots were washed with PBS supplemented with 0.1% Tween 20 four times for 5 min before staining with the secondary antibody (7076, Cell Signaling; ab216773, Abcam) in blocking buffer with 0.1% Tween 20 for 1h at room temperature ...
-
bioRxiv - Neuroscience 2024Quote: ... Sections were then washed for 5 mins in PBS and mounted with mounting media containing DAPI (catalog number ab104139, Abcam) before imaging.
-
bioRxiv - Bioengineering 2024Quote: ... for 5 minutes and then incubated for 1 hour at room temperature (RT) in NM95 primary antibody (ab190710 16 - Abcam) at 1:1500 in TBS ...
-
bioRxiv - Cancer Biology 2024Quote: ... Membranes were blocked with 5% milk-TBST for 1 hour under agitation and probed overnight for ALIX (1:1000, 186429, Abcam), Calnexin (1:1000 ...
-
bioRxiv - Cell Biology 2024Quote: ... The primary antibody was washed 2x with PBS for 5 min per wash and cells were incubated with secondary antibodies (diluted in 0.5% BSA/PBS) at room temperature in the dark for 1 h at 1:1000 dilution (anti-rabbit Alexa488, Abcam ab150077 ...
-
bioRxiv - Immunology 2020Quote: ... Sections were incubated overnight at 4°C with a rabbit polyclonal anti-mouse blocking antibody (MUC2, ab90007, Abcam Ltd; Occludin, ab168986, Abcam Ltd; caspase 3, ab44976, Abcam Ltd). The sections were washed with PBS and incubated with a secondary antibody for 30 min ...
-
bioRxiv - Molecular Biology 2021Quote: ... The membrane was blocked in 5% milk for 1h followed by incubation with primary antibodies: c-Myc (Abcam ab185656; 1:1000), Fos (Cell Signaling 2250S ...
-
bioRxiv - Developmental Biology 2021Quote: ... Sections were blocked with 5% donkey serum and incubated overnight at 4°C against MMP13 (1:100, Abcam ab39012, Cambridge, UK) and for 2 hours at room temperature with a Cy3-conjugated secondary (1:500 ...
-
bioRxiv - Neuroscience 2022Quote: ... then incubated for 2 d at 4°C in primary rabbit anti-TH antibody (Abcam, 1:200, in TBSMT + 5% DMSO). After 6 washes x 1 h with TBST ...
-
bioRxiv - Neuroscience 2021Quote: ... injected with vehicle (VEH = 0.9% aqueous solution of NaCl and 5% ethanol) or MTZ (10 mg/kg, ab120068, Abcam, Cambridge, UK). The 10 mg/kg daily dosage in mice is equivalent to 50 mg/day in humans ...
-
bioRxiv - Biochemistry 2020Quote: ... Membranes were treated with antibodies diluted 1:1000 in 0.5% BSA and washed 3 times for 5 min before applying secondary antibiotic (anti-mouse or anti-goat from Santa Cruz Biotechnology and Anti-Rabbit from Abcam at a concentration of 1:1000 ...
-
bioRxiv - Immunology 2021Quote: ... The membrane was then washed 3×15 minutes in PBST and then incubated in secondary antibody solution (PBST + 5% BSA + Goat a-Rabbit-HRP 1:5000) (Abcam, ab205718) for one hour at room temperature ...
-
bioRxiv - Neuroscience 2020Quote: ... The blots were blocked in TBST buffer containing 5% nonfat milk and then incubated overnight at 4°C with the primary rabbit polyclonal anti-Tip60 (1:1000, abcam, #ab23886), rabbit polyclonal anti-Rpd3 (1:1000 ...